Fungi play significant roles in the geochemical processes of heavy metals in the environment. However, the interaction between heavy metals and fungi, especially at the cellular level, is quite complicated and remains unknown. This study explored the mutual interaction mechanism between Pb²⁺ and Trichoderma viride by combining batch experiments, spectroscopy, and in vitro approaches. Batch experiments revealed that Pb²⁺ had toxic effect on T. viride, originally causing the biomass of T. viride decreased from 1.3 g in the control group to 0 g in the presence of 200 mg/L Pb²⁺. The difference in biomass further led to varied pH, even decreasing from 5.7 at the outset to 3.4 due to the acid-production properties of T. viride. Moreover, structural deformation and damage of T. viride mycelium appeared when exposed to Pb²⁺, and were more evident at a higher dose of Pb²⁺ exposure. The growth curve exhibited that T. viride gradually adapted to Pb²⁺ exposure, which related to Pb²⁺ exposure concentration. Further, intracellular and extracellular secretions of T. viride changed with varying exposure concentrations of Pb²⁺, indicating that T. viride adapted differently to different concentrations of Pb²⁺, and MT participated in the detoxification of T. viride. SEM-EDX showed that T. viride could bio-adsorb and bioaccumulate more Pb²⁺ when exposed to more Pb²⁺, which was closely related to the content of P. And carbonyl, phosphate, and amino groups of T. viride participated in the Pb²⁺ biosorption onto T. viride, as evidenced by FT-IR and XPS. Meanwhile, the biomineralization and reduction of Pb²⁺ by T. viride were observed by XRD and XPS, which might be a possible factor for Pb²⁺ biosorption and bioaccumulation. CLSM showed that the bio-adsorbed and bioaccumulated Pb²⁺ were mainly distributed in the membrane of T. viride mycelium.

The biological and environmental toxicity of graphene and graphene derivatives have attracted great research interest due to their increasing applications. However, the cytotoxic mechanism is poorly understood. Here, we investigated the cytotoxic effect of graphene oxide nanoribbons (GORs) on Escherichia coli (E. coli) in an in vitro method. The fabricated GORs formed long ribbons, 200 nm wide. Based on the results of the MTT assay and plate-culture experiments, GORs significantly inhibited the growth and reproduction of E. coli in a concentration-dependent manner. We found that GORs stimulated E. coli to secrete reactive oxygen species, which then oxidized and damaged the bacterial cell membrane. Moreover, interaction between GORs and E. coli cytomembrane resulted in polysaccharide adsorption by GORs and the release of lactic dehydrogenase. Furthermore, GORs effectively depleted the metal ions as nutrients in the culture medium by adsorption. Notably, mechanical cutting by GORs was not obvious, which is quite different from the case of graphene oxide sheets to E. coli.