In this study, the cytotoxicity and toxic mechanism of carbon quantum dots (CQDs) to E. coli were evaluated in vitro. The synthetic CQDs were extremely small in size (~2.08 nm) and displayed strong fluorescence. The results demonstrated that CQDs showed good biocompatibility with E. coli within a short culture time. However, when the exposure time exceeded 24 h, the toxicity of CQDs became apparent, and the contents of reactive oxygen species, lactate dehydrogenase, and the crystal violet absorption rate increased significantly. To further explore the cytotoxic mechanism, approaches including confocal laser scanning microscopy, scanning electron microscopy, and biological transmission electron microscopy combined with zeta potential tests, osmotic pressure measurement, and comet assays were performed. On the one hand, the CQDs altered the surface charges of cells and induced lipid peroxidation by adhesion on the surface of E. coli, leading to an increase in the permeability of the cell wall. On the other hand, when the concentration of CQDs reached 200 µg/mL, the osmotic pressure of the extracellular environment was significantly reduced. These are the main factors that lead to cell edema and death. Finally, the comet assays confirmed that CQDs could induce DNA damage, which could inhibit the proliferation of E. coli.

Fungi play significant roles in the geochemical processes of heavy metals in the environment. However, the interaction between heavy metals and fungi, especially at the cellular level, is quite complicated and remains unknown. This study explored the mutual interaction mechanism between Pb²⁺ and Trichoderma viride by combining batch experiments, spectroscopy, and in vitro approaches. Batch experiments revealed that Pb²⁺ had toxic effect on T. viride, originally causing the biomass of T. viride decreased from 1.3 g in the control group to 0 g in the presence of 200 mg/L Pb²⁺. The difference in biomass further led to varied pH, even decreasing from 5.7 at the outset to 3.4 due to the acid-production properties of T. viride. Moreover, structural deformation and damage of T. viride mycelium appeared when exposed to Pb²⁺, and were more evident at a higher dose of Pb²⁺ exposure. The growth curve exhibited that T. viride gradually adapted to Pb²⁺ exposure, which related to Pb²⁺ exposure concentration. Further, intracellular and extracellular secretions of T. viride changed with varying exposure concentrations of Pb²⁺, indicating that T. viride adapted differently to different concentrations of Pb²⁺, and MT participated in the detoxification of T. viride. SEM-EDX showed that T. viride could bio-adsorb and bioaccumulate more Pb²⁺ when exposed to more Pb²⁺, which was closely related to the content of P. And carbonyl, phosphate, and amino groups of T. viride participated in the Pb²⁺ biosorption onto T. viride, as evidenced by FT-IR and XPS. Meanwhile, the biomineralization and reduction of Pb²⁺ by T. viride were observed by XRD and XPS, which might be a possible factor for Pb²⁺ biosorption and bioaccumulation. CLSM showed that the bio-adsorbed and bioaccumulated Pb²⁺ were mainly distributed in the membrane of T. viride mycelium.