Cell-type specific gene silencing by histone H3 lysine 27 and lysine 9 methyltransferase complexes PRC2 and G9A-GLP is crucial both during development and to maintain cell identity. Although studying their interaction partners has yielded valuable insight into their functions, how these factors are regulated on a network level remains incompletely understood. Here, we present a new approach that combines quantitative interaction proteomics with global chromatin profiling to functionally characterize repressive chromatin modifying protein complexes in embryonic stem cells. We define binding stoichiometries of 9 new and 12 known interaction partners of PRC2 and 10 known and 29 new interaction partners of G9A-GLP, respectively. We demonstrate that PRC2 and G9A-GLP interact physically and share several interaction partners, including the zinc finger proteins ZNF518A and ZNF518B. Using global chromatin profiling by targeted mass spectrometry, we discover that even sub-stoichiometric binding partners such as ZNF518B can positively regulate global H3K9me2 levels. Biochemical analysis reveals that ZNF518B directly interacts with EZH2 and G9A. Our systematic analysis suggests that ZNF518B may mediate the structural association between PRC2 and G9A-GLP histone methyltransferases and additionally regulates the activity of G9A-GLP.

Nowadays, vehicles with alternative drives are playing an increasingly important role in road transport. Among the various types of alternative-drives, the most widespread ones are hybrid and electric ones, thanks for the rapid development of modern batteries, and hybrid and electrical systems. The above fact establishes the importance of research on various types of electric motors. The Faculty of Engineering of the University of Debrecen has more than a decade of experience in developing prototype race cars with pneumatic and electric drive. For the more effective and conscious development and racing a simulation program has been developed in MATLAB/Simulink environment for the computation of the dynamic functions of a vehicle moving in a linear path. The program is characterized by a modular structure, so the different structural units (vehicle body, front and rear wheels, power train, motor) are modelled and simulated separately. In this study we present models and simulation programs for different electric motor types (series-wound DC and brushless DC motors) in MATLAB/Simulink and apply them in our vehicle dynamics simulation program. From the simulation results the performance of a vehicle – driven by an electric motor – can be predicted in a race situation, consequently the most suitable motor with the optimal characteristics can be selected to it.

Previously, a dynamic model and a simulation program for series wound DC motors (SWDCM) were developed in MATLAB/Simulink for modelling them in electric vehicles and mechatronic systems. The electromagnetic characteristics of the motor (electric resistances, dynamic inductances), which serve as input parameters of the program, were also measured. Additionally, locked rotor response measurements were performed to test the accuracy of the measured electromagnetic characteristics. This paper presents the experimental procedure and the results of dynamics test measurements that were performed on the same motor, including the procedure for the determination of the necessary input dynamic parameters for the simulation. While the motor spins up from rest, the intensity of the electric current and the angular speed of the rotor are measured. Finally, the simulation and dynamic test results are compared to check the proper operation of the simulation program.

The Faculty of Engineering of the University of Debrecen has a long-standing tradition of developing electric and pneumatic-driven prototype race cars. These vehicles are regular participants in domestic and international university competitions. For more conscious development, thus, for more successful racing, a vehicle dynamics simulation program was developed by our research group. One of the main parts of the above program is the simulation of the drive system, including the electric motor. The input data for the motor simulation programs are the electromagnetic and dynamic characteristics of the motor. Most of these characteristics are usually not specified in the motor catalogue; thus, they have to be measured. In this paper, a detailed description of a recently developed measurement system (MS), which is capable of measuring all the above-mentioned characteristics, is presented. Additionally, by applying it, test measurements can also be performed on the motors to check the accuracy of the output functions generated by the simulation programs. Several experimental arrangements and procedures for specific experimental tasks are also presented here as examples for the application of the MS.

At the Faculty of Engineering of the University of Debrecen we have dealt with the design and construction of electric prototype race cars for more than a decade. With a focus on more conscious design and racing we developed a vehicle dynamics simulation program, which can be used to generate the dynamics functions of the cars from their technical characteristics and data. In this publication, we deal with the optimization of the technical parameters of the above-mentioned cars for various competition tasks using our simulation program. This is a completely new field of application of the used optimization methods. This concept and idea can effectively help student teams all over the world to prepare for various domestic and international competitions. One of the applied methods is a graphic procedure, the other one is the widely used “adaptive simulated annealing” (ASA). After a brief description of the simulation program, the applied optimization methods and developed MATLAB codes for them are described. Finally, to demonstrate the effectiveness of the methods, we optimize the parameters of a prototype race car for different competition tasks and present the obtained results.