Leaf area index (LAI) estimation is very important, and not only for canopy structure analysis and yield prediction. The unmanned aerial vehicle (UAV) serves as a promising solution for LAI estimation due to its great applicability and flexibility. At present, vegetation index (VI) is still the most widely used method in LAI estimation because of its fast speed and simple calculation. However, VI only reflects the spectral information and ignores the texture information of images, so it is difficult to adapt to the unique and complex morphological changes of rice in different growth stages. In this study we put forward a novel method by combining the texture information derived from the local binary pattern and variance features (LBP and VAR) with the spectral information based on VI to improve the estimation accuracy of rice LAI throughout the entire growing season. The multitemporal images of two study areas located in Hainan and Hubei were acquired by a 12-band camera, and the main typical bands for constituting VIs such as green, red, red edge, and near-infrared were selected to analyze their changes in spectrum and texture during the entire growing season. After the mathematical combination of plot-level spectrum and texture values, new indices were constructed to estimate rice LAI. Comparing the corresponding VI, the new indices were all less sensitive to the appearance of panicles and slightly weakened the saturation issue. The coefficient of determination (R²) can be improved for all tested VIs throughout the entire growing season. The results showed that the combination of spectral and texture features exhibited a better predictive ability than VI for estimating rice LAI. This method only utilized the texture and spectral information of the UAV image itself, which is fast, easy to operate, does not need manual intervention, and can be a low-cost method for monitoring crop growth.

The accurate estimation of rice yield using remote sensing (RS) technology is crucially important for agricultural decision-making. The rice yield estimation model based on the vegetation index (VI) is commonly used when working with RS methods, however, it is affected by irrelevant organs and background especially at heading stage. The spectral mixture analysis (SMA) can quantitatively obtain the abundance information and mitigate the impacts. Furthermore, according to the spectral variability and information complexity caused by the rice cropping system and canopy characteristics of reflection and scattering, in this study, the multi-endmember extraction by the pure pixel index (PPI) and the nonlinear unmixing method based on the bandwise generalized bilinear mixing model (NU-BGBM) were applied for SMA, and the VI_E (VIs recalculated from endmember spectra) was integrated with abundance data to establish the yield estimation model at heading stage. In two paddy fields of different cultivation settings, multispectral images were collected by an unmanned aerial vehicle (UAV) at booting and heading stage. The correlation of several widely-used VIs and rice yield was tested and weaker at heading stage. In order to improve the yield estimation accuracy of rice at heading stage, the VI_E and foreground abundances from SMA were combined to develop a linear yield estimation model. The results showed that VI_E incorporated with abundances exhibited a better estimation ability than VI alone or the product of VI and abundances. In addition, when the structural difference of plants was obvious, the addition of the product of VI_F (VIs recalculated from bilinear endmember spectra) and the corresponding bilinear abundances to the original product of VI_E and abundances, enhanced model reliability. VIs using the near-infrared bands improved more significantly with the estimation error below 8.1%. This study verified the validation of the targeted SMA strategy while estimating crop yield by remotely sensed VI, especially for objects with obvious different spectra and complex structures.

The pigment content of rice leaves plays an important role in the growth and development of rice. The accurate and rapid assessment of the pigment content of leaves is of great significance for monitoring the growth status of rice. This study used the Analytical Spectra Device (ASD) FieldSpec 4 spectrometer to measure the leaf reflectance spectra of 4 rice varieties during the entire growth period under 4 nitrogen application rates and simultaneously measured the leaf pigment content. The leaf’s absorption spectra were calculated based on the physical process of spectral transmission. An examination was conducted on the variations in pigment composition among distinct rice cultivars, alongside a thorough dissection of the interrelations and distinctions between leaf reflectance spectra and absorption spectra. Based on the vegetation index proposed by previous researchers in order to invert pigment content, the absorption spectrum was used to replace the original reflectance data to optimize the vegetation index. The results showed that the chlorophyll and carotenoid contents of different rice varieties showed regular changes during the whole growth period, and that the leaf absorption spectra of different rice varieties showed more obvious differences than reflectance spectra. After replacing the reflectance of pigment absorptivity-sensitive bands (400 nm, 550 nm, 680 nm, and red-edge bands) with absorptivities that would optimize the vegetation index, the correlation between the vegetation index, which combines absorptivity and reflectivity, and the chlorophyll and carotenoid contents of 4 rice varieties during the whole growth period was significantly improved. The model’s validation results indicate that the pigment inversion model, based on the improved vegetation index using absorption spectra, outperforms the traditional vegetation index-based pigment inversion model. The results of this study demonstrate the potential application of absorption spectroscopy in the quantitative inversion of crop phenotypes.