High concentrations of copper (Cu²⁺) pose a great threat to aquatic animals. However, the mechanisms underlying the response of crustaceans to Cu²⁺ exposure have not been well studied. Therefore, we investigated the alterations of physiological and molecular parameters in Chinese mitten crab (Eriocheir sinensis) after Cu²⁺ exposure. The crabs were exposed to 0 (control), 0.04, 0.18, and 0.70 mg/L of Cu²⁺ for 5 days, and the hemolymph, hepatopancreas, gills, and muscle were sampled. The results showed that Cu²⁺ exposure decreased the antioxidative capacity and promoted lipid peroxidation in different tissues. Apoptosis was induced by Cu²⁺ exposure, and this activation was associated with the mitochondrial and ERK pathways in the hepatopancreas. ER stress-related genes were upregulated in the hepatopancreas but downregulated in the gills at higher doses of Cu²⁺. Autophagy was considerably influenced by Cu²⁺ exposure, as evidenced by the upregulation of autophagy-related genes in the hepatopancreas and gills. Cu²⁺ exposure also caused an immune response in different tissues, especially the hepatopancreas, where the TLR2-MyD88-NF-κB pathway was initiated to mediate the inflammatory response. Overall, our results suggest that Cu²⁺ exposure induces oxidative stress, ER stress, apoptosis, autophagy, and immune response in E. sinensis, and the toxicity may be implicated following the activation of the ERK, AMPK, and TLR2-MyD88-NF-κB pathways.

Dietary selenium (Se) is an essential component that supports fish growth and the immune system. This review attempts to provide insight into the biological impacts of dietary Se, including immunological responses, infection defense, and fish species growth, and it also identifies the routes via which it enters the aquatic environment. Dietary Se is important in fish feed due to its additive, antioxidant, and enzyme properties, which aid in various biological processes. However, excessive intake of it may harm aquatic ecosystems and potentially disrupt the food chain. This review explores the diverse natures of dietary Se, their impact on fish species, and the biological methods for eliminating excesses in aquatic environments. Soil has a potential role in the distribution of Se through erosion from agricultural, industrial, and mine sites. The research on dietary Se’s effects on fish immune system and growth can provide knowledge regarding fish health, fish farming strategies, and the health of aquatic ecosystems, promoting the feed industry and sustainable aquaculture. This review provides data and references from various research studies on managing Se levels in aquatic ecosystems, promoting fish conservation, and utilizing Se in farmed fish diets.

Aquaculture, the world’s fastest-growing food production sector, is critical for addressing food security concerns because of its potential to deliver high-quality, nutrient-rich supplies by 2050. This review assesses the effectiveness of CRISPR/Cas9 genome editing technology in enhancing desirable traits in fish species, including growth rates, muscle quality, disease resistance, pigmentation, and more. It also focuses on the potential effectiveness of the technology in allowing precise and targeted modifications of fish DNA to improve desirable characteristics. Many studies have reported successful applications of CRISPR/Cas9, such as knocking out reproductive genes to control reproduction and sex determination, enhancing feed conversion efficiency, and reducing off-target effects. Additionally, this technology has contributed to environmental sustainability by reducing nitrogen-rich waste and improving the nutritional composition of fish. However, the acceptance of CRISPR/Cas9 modified fish by the public and consumers is hindered by concerns regarding public perception, potential ecological impacts, and regulatory frameworks. To gain public approval and consumer confidence, clear communication about the editing process, as well as data on the safety and environmental considerations of genetically modified fish, are essential. This review paper discusses these challenges, provides possible solutions, and recommends future research on the integration of CRISPR/Cas9 into sustainable aquaculture practices, focusing on the responsible management of genetically modified fish to enable the creation of growth and disease-resistant strains. In conclusion, this review highlights the transformative potential of CRISPR/Cas9 technology in improving fish traits, while also considering the challenges and ethical considerations associated with sustainable and responsible practices in aquaculture.

The growing global interest in sustainable aquaculture has led to an increased search for alternatives to synthetic antibiotics and chemical feed additives. Medicinal plants have surfaced as promising ecological solutions, with research showing their potential to improve fish growth, boost immunity, and enhance disease resistance while decreasing the need for antibiotics. Recent studies suggest that supplementing fish diets with medicinal plants may improve survival rates. This supplementation upregulates key immune‐related gene responses, including cytokines such as interleukin (IL)‐1 β and tumor necrosis factor‐alpha (TNF‐ α ), as well as lysozyme (LYZ and LZM genes) and immunoglobulins (IgM, IgT, and IgD). As a result, innate and adaptive immune responses can be enhanced depending on the species and dosage. Furthermore, certain plant‐derived compounds have shown antimicrobial properties similar to traditional antibiotics, reducing pathogen loads by more than 50%. This review examines the role of medicinal plants in aquaculture, highlighting their effects on immunity, antimicrobial activity, and growth promotion. It delves into the mechanisms involved, such as the modulation of cytokine expression, enhancement of antioxidant defenses, and regulation of gut microbiota. However, challenges like variable phytochemical composition, the need for optimal dosages, and regulatory issues limit their broader use. While previous reviews have addressed the use of medicinal plants in aquaculture, few have thoroughly examined their dual role in both enhancing fish health and contributing to ecological functions such as improving water quality and promoting climate resilience. This review aims to fill that critical knowledge gap by integrating these dimensions highlighting how medicinal plants serve not only as bioactive growth promoters and immune enhancers but also as ecological tools for fostering environmental sustainability in aquaculture systems. Incorporating medicinal plants into aquaculture could potentially reduce antibiotic use by up to 50% by 2050, aligning with global efforts toward sustainable and eco‐friendly fish production. Innovations in phytochemical profiling, nanotechnology‐based delivery systems, and next‐generation sequencing are anticipated to further enhance the effectiveness and standardization of plant‐based aquafeeds. This comprehensive framework offers new insights into the development of resilient and environmentally responsible aquaculture practices, particularly in the face of climate change.

In order to identify candidate genes or loci associated with growth performance of the newly established common carp strain, Xinlong, we conducted a genome-wide association analysis using 2b-RAD technology on 123 individuals. We constructed two sets of libraries associated with growth-related parameters (weight, length, width and depth) measured at two different grow-out stages. Among the 413,059 SNPs identified using SOAP SNP calling, 147,131 were tested for GWAS after quality filtering. Finally, 39 overlapping SNPs, assigned to four genomic locations, were associated with growth traits in two stages. These loci were assigned to functional classes related to immune response, response to stress, neurogenesis, cholesterol metabolism and development, and proliferation and differentiation of cells. By overlapping results of Plink and EMMAX analyses, we identified three genes: TOX, PLK2 and CD163 (both methods P < 0.05). Our study results could be used for marker-assisted selection to further improve the growth of the Xinlong strain, and illustrate that largely different sets of genes drive the growth of carp in the early and late grow-out stages.

Hypothermia-exposure-induced oxidative stress dysregulates cell fate and perturbs cellular homeostasis and function, thereby disturbing fish health. To evaluate the impact of hypothermia on the freshwater drum (Aplodinotus grunniens), an 8-day experiment was conducted at 25 °C (control group, Con), 18 °C (LT18), and 10 °C (LT10) for 0 h, 8 h, 1 d, 2 d, and 8 d. Antioxidant and non-specific immune parameters reveal hypothermia induced oxidative stress and immunosuppression. Liver ultrastructure alterations indicate hypothermia induced mitochondrial enlargement, nucleoli aggregation, and lipid droplet accumulation under hypothermia exposure. With the analysis of the transcriptome, differentially expressed genes (DEGs) induced by hypothermia were mainly involved in metabolism, immunity and inflammation, programmed cell death, and disease. Furthermore, the inflammatory response and apoptosis were evoked by hypothermia exposure in different immune organs. Interactively, apoptosis and inflammation in immune organs were correlated with antioxidation and immunity suppression induced by hypothermia exposure. In conclusion, these results suggest hypothermia-induced inflammation and apoptosis, which might be the adaptive mechanism of antioxidation and immunity in the freshwater drum. These findings contribute to helping us better understand how freshwater drum adjust to hypothermia stress.