Guar is a commercially important legume crop known for guar gum. Guar is tolerant to various abiotic stresses, but the mechanisms involved in its salinity tolerance are not well established. This study aimed to understand molecular mechanisms of salinity tolerance in guar. RNA sequencing (RNA-Seq) was employed to study the leaf and root transcriptomes of salt-tolerant (Matador) and salt-sensitive (PI 340261) guar genotypes under control and salinity. Our analyses identified a total of 296,114 unigenes assembled from 527 million clean reads. Transcriptome analysis revealed that the gene expression differences were more pronounced between salinity treatments than between genotypes. Differentially expressed genes associated with stress-signaling pathways, transporters, chromatin remodeling, microRNA biogenesis, and translational machinery play critical roles in guar salinity tolerance. Genes associated with several transporter families that were differentially expressed during salinity included ABC, MFS, GPH, and P-ATPase. Furthermore, genes encoding transcription factors/regulators belonging to several families, including SNF2, C₂H₂, bHLH, C3H, and MYB were differentially expressed in response to salinity. This study revealed the importance of various biological pathways during salinity stress and identified several candidate genes that may be used to develop salt-tolerant guar genotypes that might be suitable for cultivation in marginal soils with moderate to high salinity or using degraded water.

Guar (Cyamopsis tetragonoloba (L.) Taub.) is a legume crop, and gum derived from its seeds has various industrial applications. Due to its tolerance to various abiotic stresses, guar can be grown under water-deficit or high-salinity conditions. In this investigation, four diverse guar genotypes that performed at a similar level in field conditions were evaluated in a salinity experiment in the greenhouse lysimeter system. Based on the salt tolerance index (STI) for shoot biomass, root biomass, shoot length, and root length, Matador and PI 268229 were classified as salt-tolerant, and PI 340261 and PI 537281 as salt-sensitive. Leaf Na concentrations were 4- to 5.5-fold higher, and leaf Cl concentrations were 1.6- to 1.9-fold higher in salt-sensitive lines than salt-tolerant lines under salinity. The strong associations between the leaf K concentrations under salinity compared to the control (K-salinity/K-control) ratio and STI for stem and root length advocate higher importance of K-salinity/K-control than total leaf K concentrations. The expression analyses of genes involved in Na⁺ and Cl⁻ transport revealed the importance of different component traits of salinity tolerance mechanisms, such as the exclusion of Na⁺/Cl⁻ from the root, sequestration of Cl⁻ in root vacuoles, retrieval of Na⁺/Cl⁻ from xylem during salinity stress, root-to-shoot Na⁺/Cl⁻ translocation, and K⁺-Na⁺ homeostasis.

Guar is a potential crop that can be grown as a forage or as a seed crop in arid to semi-arid regions due to its low water requirements and tolerance to heat. Optimizing irrigation water use is important for making alternative crops such as guar a sustainable option. Amendments such as biogenic silica, a sedimentary rock from a biogenic source such as fossils, may help plants tolerate water stress due to reduced irrigation. The objective of the current study was to evaluate seed yield and attribute components and agronomic and physiological parameters for four guar genotypes (Matador, Kinman, Lewis, and NMSU 15-G1) under five drip irrigation regimes (I₁-normal irrigation, I₂-no irrigation at 75% pod formation, I₃-no irrigation at 50% and 75% pod formation, I₄-terminate irrigation at flowering, and I₅-terminate irrigation at flowering + biogenic silica amendment) at Las Cruces in southern New Mexico, USA, from 2016 to 2018. On average, the I₁ irrigation regime produced the highest guar seed yield (2715 kg ha⁻¹) followed by I₅ (2469 kg ha⁻¹) from 2016 to 2018. As compared to the I₁ regime, the I₂ and I₃ regimes resulted in a 20.8% and 23.4% decline in guar seed yield, respectively, on average from 2016 to 2018. The results suggest that the addition of biogenic silica might help to improve guar seed yield under reduced irrigation conditions and can produce comparable yields with an average of 300 mm of irrigation during the growing season in the southern New Mexico region of the Southwest US.