Search and rescue dogs are an important link in the search for missing persons. The aim of the study was to assess exam stress in search and rescue dogs and their handlers. The study included 41 rescue teams taking exams of field and rubble specialties. The level of cortisol, which is the main glucocorticosteroid modulating stress reactions in humans and dogs, was analyzed. The biological material used to assess the hormone concentration was saliva collected in a non-invasive way. In total, 164 test samples were collected: two from the dog and two from the handler before and immediately after the exam. Rescue exams were shown to significantly increase salivary cortisol in both dogs and their handlers. Strong interactions between cortisol levels in human–dog teams were also found with a more pronounced effect in female dog–female handler dyads.

Enriching cats’ living environment in shelters is crucial in reducing their stress. Easier access to resources allows cats to display natural behavior. This study aimed to assess whether cats staying in an enriched environment would be less stressed than cats staying in a standard environment. The first group consisted of cats living in an environment with fewer resources (standard environment)—103 cats. The second group consisted of cats living in an enriched environment—76 cats. The research material consisted of hair collected to determine the cortisol level. The results indicate that cats from a more enriched environment have almost half the level of cortisol in hair than cats from an environment with fewer resources (0.059 ng/mg vs. 0.101 ng/mg; p = 0.000001).

It has been assumed that stroking relieves stress responses in dogs, and dogs with the activation of the left-brain hemisphere (right-pawed) may show better adaptation to stress conditions. The aim of the study was to determine whether the stroking stimulus induced changes in the level of selected neuroregulators in dogs’ blood and whether these changes depended on the sex and the predominance of the activity of one of the brain hemispheres. The study involved 40 dogs of various breeds and both sexes. The experimental animals were subjected to a behavioral tests (Kong test), and the levels of noradrenaline, serotonin, and cortisol were determined in their blood plasma. The results of the behavioral test revealed that most dogs exhibited increased activity of the left hemisphere. Furthermore, irrespective of the sex and paw preference, stroking the animal was found to alleviate the stress response, which was reflected in reduced cortisol levels and increased serotonin levels. It was found that the plasma noradrenaline, cortisol, and serotonin levels were lower in the female dogs than in the males. Additionally, the plasma noradrenaline and serotonin levels were higher in the right-pawed dogs than in the left-pawed dogs. The present results confirm the assumption that right-pawed dogs adapt to stressful conditions more readily.

We developed an algorithm to classify brown hares into two age classes, juveniles (up to 1 year old) and adults (over 1 year old), based on body weight, which can be determined by both the examination of live animals and postmortem analysis. Considering the strong correlation between lens weight and carcass weight, we assumed that hares could be classified into one of the two age groups based only on carcass weight, using a logistic regression model. Using logistic regression, a model was constructed to assess the age of hares based on their body weight. For comparison with the current age-assessment method based on the dry lens weight, a logistic regression classifying the hares based on the dry lens weight was performed as well. The results of the study facilitated the development of a method to classify hares into age groups based on body weight. The proposed approach is innovative, as it allows for the determination of the age of not only culled (postmortem) but also live hares. The method is easy and does not require laboratory tests; hence, the results can be used immediately following evaluation. This method allows hares to be categorized into two age groups (juveniles and adults). With an accuracy of 97.52% and 95.45% in the case of juvenile and adult hares, respectively, the proposed approach can be widely used both in population management and scientific research.

This study presents the hematological and serum biochemical values of physically captured roe deer (Capreolus capreolus). The study material was blood sampled from roe deer captured with the use of a specialist trap mesh and no anesthesia. Blood samples were collected from 122 roe deer from January to April in 2016, 2017, and 2018 in the Lublin region of Poland. The hematological and biochemical variables were determined with results showing statistically significant differences between the mean values of RBC, HCT, and HB according to sex. Reference intervals should be more specific to the broad physical versus chemical capture categories.

The aim of this study was to assess salivary cortisol level in dogs during admission and a three-day stay in a pet hotel. The study involved 20 dogs admitted to a pet hotel for a stay lasting for at least three consecutive days. The study group consisted of 10 small dogs and 10 medium-sized dogs, eight females and 12 males. Saliva was collected each day to assess cortisol levels. A stay in a hotel is a stressful situation for dogs, as evidenced by the increase in the salivary cortisol level. The highest salivary cortisol level was observed on the day of admission to the hotel. The median salivary cortisol level in the dogs on the consecutive days was 2.7 ng/ml on admission, 2.1 ng/ml after 24 hours, and 1.9 ng/ml after 48 hours. Depending on the size of the dog, the median salivary cortisol level was 2.8 ng/ml, 2.9 ng/ml and 2.4 ng/ml in the small dogs and and 2.2 ng/ml, 1.7 ng/ml and 1.2 ng/ml in the medium sized dogs on the consecutive days.