Search and rescue dogs are an important link in the search for missing persons. The aim of the study was to assess exam stress in search and rescue dogs and their handlers. The study included 41 rescue teams taking exams of field and rubble specialties. The level of cortisol, which is the main glucocorticosteroid modulating stress reactions in humans and dogs, was analyzed. The biological material used to assess the hormone concentration was saliva collected in a non-invasive way. In total, 164 test samples were collected: two from the dog and two from the handler before and immediately after the exam. Rescue exams were shown to significantly increase salivary cortisol in both dogs and their handlers. Strong interactions between cortisol levels in human–dog teams were also found with a more pronounced effect in female dog–female handler dyads.

Objectives: The aim of the study was to analyse hair cortisol levels in cats whose owners had reported behavioural problems and undesirable behaviour. In addition, feline hair cortisol levels were compared between sexes, for indoor and outdoor cats and also for cats living in single or multi-cat households. Methods: In total, 55 cats participated in the study, including 31 females and 24 males from various households. The cats belonged to 25 different owners who answered a questionnaire survey. The biological material used for the research were 2–3 cm fragments of hair removed at the level of the skin with scissors by the owners from the cats’ lumbosacral area. The cortisol concentration in the samples was determined with the DRG Salivary Cortisol HS ELISA. Statistical analysis of the results was performed with Statistica 13.3. Results: A tendency toward lower cortisol levels was found in outdoor cats vs indoor cats (U = 251.5, P = 0.066). It was also found that the cats exhibiting behavioural problems (eg, house soiling [eliminating outside the litter box]) or aggressive behaviour towards household members had statistically significantly higher hair cortisol levels (U = 162.0 [ P = 0.027] and U = 9.0 [ P = 0.040], respectively). Conclusions and relevance: High levels of stress in cats can affect their interactions with other cats and with humans. The incidence of undesirable behaviour was observed more frequently in indoor cats. Significantly higher cortisol levels were found in cats that eliminated outside the litter box or that showed aggression towards their owners.

Human–animal interactions and the emotional relationship of the owner with the pet are the subjects of many scientific studies and the constant interest of not only scientists but also pet owners. The aim of this study was to determine and compare the hair cortisol levels of dogs, cats, and their owners living in the same household. The owners were asked to complete a questionnaire concerning the frequency of their interactions with pets and emotional relationship with each of their cats and each of their dogs. The study involved 25 women who owned at least one dog and at least one cat. In total, 45 dogs and 55 cats from 25 households participated in the study. The average level of hair cortisol of the owners was 4.62 ng/mL, of the dogs 0.26 ng/mL, and in the hair of cats 0.45 ng/mL. There was no significant correlation between the hair cortisol level of the owner and dog or the owner and the cat and between dogs and cats living together. A significant positive correlation was observed between the hair cortisol level in the owner and the pet, for dogs in which the owner performs grooming treatments once a week and for cats which are never kissed. Although our study did not find many significant correlations, studies using other stress markers might have yielded different results.

Enriching cats’ living environment in shelters is crucial in reducing their stress. Easier access to resources allows cats to display natural behavior. This study aimed to assess whether cats staying in an enriched environment would be less stressed than cats staying in a standard environment. The first group consisted of cats living in an environment with fewer resources (standard environment)—103 cats. The second group consisted of cats living in an enriched environment—76 cats. The research material consisted of hair collected to determine the cortisol level. The results indicate that cats from a more enriched environment have almost half the level of cortisol in hair than cats from an environment with fewer resources (0.059 ng/mg vs. 0.101 ng/mg; p = 0.000001).