In this review, we discuss microbiological and molecular concepts of Microbially Induced Calcium Carbonate Precipitation (MICP) and their role in bioconcrete. MICP is a widespread biochemical process in soils, caves, freshwater, marine sediments, and hypersaline habitats. MICP is an outcome of metabolic interactions between diverse microbial communities with organic and/or inorganic compounds present in the environment. Some of the major metabolic processes involved in MICP at different levels are urea hydrolysis, denitrification, dissimilatory sulfate reduction, and photosynthesis. Currently, MICP directed by urea hydrolysis, denitrification, and dissimilatory sulfate reduction has been reported to aid in the development of bioconcrete and has demonstrated an improvement in the mechanical and durability properties of concrete. Bioconcrete is a promising sustainable technology which reduces negative environmental impact caused by CO₂ emissions from the construction sector, as well as in terms of economic benefits by way of promoting a self-healing process of concrete structures. Among the metabolic processes mentioned above, urea hydrolysis is the most applied in concrete repair mechanisms. MICP by urea hydrolysis is induced by a series of reactions driven by urease (Ur) and carbonic anhydrase (CA). Catalytic activity of these two enzymes depends on diverse parameters, which are currently being studied under laboratory conditions to better understand the biochemical mechanisms involved and their regulation in microorganisms. It is clearly evident that microbiological and molecular components are essential to improving the process and performance of bioconcrete.

Lignocellulosic biomass is recalcitrant due to its heterogeneous structure, which is one of the major limitations for its use as a feedstock for methane production. Although different pretreatment methods are being used, intermediaries formed are known to show adverse effect on microorganisms involved in methane formation. This review, apart from highlighting the efficiency and limitations of the different pretreatment methods from engineering, chemical, and biochemical point of views, will discuss the strategies to increase the carbon recovery in the form of methane by way of amending pretreatments to lower inhibitory effects on microbial groups and by optimizing process conditions.

Pesticides are chemicals used in agriculture, forestry, and, to some extent, public health. As effective as they can be, due to the limited biodegradability and toxicity of some of them, they can also have negative environmental and health impacts. Pesticide biodegradation is important because it can help mitigate the negative effects of pesticides. Many types of microorganisms, including bacteria, fungi, and algae, can degrade pesticides; microorganisms are able to bioremediate pesticides using diverse metabolic pathways where enzymatic degradation plays a crucial role in achieving chemical transformation of the pesticides. The growing concern about the environmental and health impacts of pesticides is pushing the industry of these products to develop more sustainable alternatives, such as high biodegradable chemicals. The degradative properties of microorganisms could be fully exploited using the advances in genetic engineering and biotechnology, paving the way for more effective bioremediation strategies, new technologies, and novel applications. The purpose of the current review is to discuss the microorganisms that have demonstrated their capacity to degrade pesticides and those categorized by the World Health Organization as important for the impact they may have on human health. A comprehensive list of microorganisms is presented, and some metabolic pathways and enzymes for pesticide degradation and the genetics behind this process are discussed. Due to the high number of microorganisms known to be capable of degrading pesticides and the low number of metabolic pathways that are fully described for this purpose, more research must be conducted in this field, and more enzymes and genes are yet to be discovered with the possibility of finding more efficient metabolic pathways for pesticide biodegradation.