The Apiaceae family, known for aromatic plants producing bioactive essential oils (EOs), holds significance across sectors, including agrochemicals. This study evaluated the insecticidal potential of four Apiaceae EOs from Crithmum maritimum L., Trachyspermum ammi (L.) Sprague ex Turrill, Smyrnium olusatrum L., and Elwendia persica (Boiss.) Pimenov and Kljuykov against various significant storage pests (Sitophilus oryzae (L.), Trogoderma granarium Everts, Rhyzopertha dominica (F.), Tribolium castaneum (Herbst), T. confusum Jacquelin du Val, Oryzaephilus surinamensis (L.), Alphitobius diaperinus (Panzer), Acarus siro L., and Tenebrio molitor L.) on wheat. Insect mortality rates were monitored at intervals of 1, 2, 3, 4, 5, 6, and 7 days. Smyrnium olusatrum EO exhibited the highest efficacy, followed by T. ammi, C. maritimum, and E. persica EOs, although efficacy varied by species, developmental stage, and concentration. Notably, complete mortality occurred for several pests at 1000 ppm of S. olusatrum and T. ammi EOs. Gas chromatography–mass spectrometry (GC–MS) analysis revealed key compounds in these EOs, including myrcene, germacrone, and curzerene in S. olusatrum EO, and thymol, γ-terpinene, and p-cymene in T. ammi EO. These findings emphasize their potential as botanical insecticides. Smyrnium olusatrum and T. ammi EOs emerge as promising eco-friendly pest management options due to their efficacy, highlighted compound composition, and availability of biomass from both wild and cultivated sources.

Tribolium castaneum, Trogoderma granarium, Oryzaephilus surinamensis, Sitophilus oryzae, Rhyzopertha dominica, and Cryptolestes ferrugineus are all major pests of stored grains. In this study, the efficiency of single and joint applications of the entomopathogenic nematode (EPN) Steinernema carpocapsae at two different doses (50 and 100 IJs cm⁻²) and the entomopathogenic fungus (EPF) Beauveria bassiana for the management of the aforementioned pests was estimated. At single treatments, both doses of S. carpocapsae caused higher mortality rates to all six pest species compared to B. bassiana. The combined treatment of EPF and EPN resulted in higher mortality compared to single treatments. Mortality was strongly influenced by the exposure interval and the application dose of the EPN at both single and combined treatments. Maximum mortality was observed for the application of the combined treatment at the high dose of S. carpocapsae and B. bassiana. Among the different insect species tested, the maximum mortality rate was observed for R. dominica (96.62%), followed by S. oryzae (90.48%), T. castaneum (87.23%), C. ferrugineus (76.05%), O. surinamensis (70.74%), and T. granarium (57.71%). The outcomes of this study demonstrate the potential of utilizing specific combinations of EPF and EPN as effective natural enemies against stored-grain pests.

Sitophilus oryzae, Tribolium castaneum, Tribolium confusum, Oryzaephilus surinamensis, Rhyzopertha dominica, Tenebrio molitor, Trogoderma granarium, Acarus siro, and Alphitobius diaperinus represent significant arthropod stored-product pests worldwide. To combat these noxious arthropods, the current study examines the pesticidal effect of essential oils (EOs) derived from four aromatic plants, i.e., Illicium verum Hook. F., Citrus reticulata Blanco, Monodora myristica (Gaertn.) Dunal, and Xylopia aethiopica (Dunal) A. Rich. Considering the challenge of pesticide resistance, the current study focuses on assessing the efficacy of these EOs as an eco-friendly alternative to traditional synthetic insecticides. Two EO concentrations (500 and 1000 µL/kg wheat) were applied to different life stages of these pests in the bioassays. Mortality rates were monitored over several days under controlled environmental conditions. The findings demonstrated that C. reticulata and I. verum EOs had elevated insecticidal effects, especially against larval stages, resulting in 100% mortality in several species. On the contrary, M. myristica and X. aethiopica EOs showed less overall efficacy despite their potency against some pests. Both I. verum and C. reticulata EOs outperformed the positive control, pirimiphos-methyl, in several assays. The results of the current study highlight the potential of several EOs as effective alternatives in reducing synthetic pesticide use for integrated pest control management.

This study determined the efficacy of Beauveria bassiana (Bals. -Criv.) Vuill., Metarhizium anisopliae (Metchnikoff) Sorokin, and diatomaceous earth mixed with abamectin (DEA) alone and in their combinations for the integrated management of larvae and adults of Tribolium castaneum (Herbst) from three field populations of Pakistan (Multan, Rawalpindi, and Rahim Yar Khan) and one laboratory population (Faisalabad). Treatments were applied on three surfaces, namely, viz. steel, concrete, and jute bags, implementing two application methods, dusting and spraying. The combined treatments were more effective in comparison with single treatments for both larvae and adults. Overall, the highest mortality rates were recorded in the Faisalabad population, followed by the Rehaim Yar Khan, Rawalpindi, and Multan populations. Progeny production was suspended 21 days after exposure to the combined treatment of DEA and both fungi in all populations except Rawalpindi. Larvae were found to be more susceptible than adults in all treatments and intervals. Dusting was more efficient than spraying for both larvae and adults and for all the populations studied. The present study provides a wholistic understanding of the impact of different factors on the success of the combined treatments using DEA and entomopathogenic fungi, supporting their use as surface treatments.

Chlorantraniliprole, an anthranilic diamide insecticide, has emerged as a promising solution for controlling agricultural pests because of its low mammalian toxicity and selectivity towards non-target organisms. This study investigated the sublethal effects of chlorantraniliprole on the mobility behavior of two significant stored-product pests, Sitophilus oryzae (L.) and Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae). Contact toxicity assays revealed varying susceptibility levels between the two species, with S. zeamais showing higher sensitivity. Subsequent analysis of mobility behavior, both in the presence and absence of food, indicated significant differences between chlorantraniliprole-exposed and control groups. While S. oryzae exhibited altered locomotion patterns and a decreased number of food approaches at sublethal concentrations, S. zeamais displayed increased walking time and reduced immobility periods. These findings highlight the importance of considering sublethal effects in understanding the overall impact of chlorantraniliprole on stored-product pests. Further research into the long-term consequences of sublethal exposure is warranted to inform more effective pest management strategies in storage.