Chronic Bee Paralysis Virus (CBPV), a widespread honey bee RNA virus, causes massive worker bee losses, mostly in strong colonies. Two different syndromes, with paralysis, ataxia and flight incapacity on one hand and black hairless individuals with shortened abdomens on the other, can affect a colony simultaneously. This case report presents two Apis mellifera carnica colonies with symptoms of paralysis and hairless black syndrome in 2019. Via RT-PCR, a highly positive result for CBPV was detected in both samples. Further problems, such as a Nosema infection and Varroa infestation, were present in these colonies. Therapy methods were applied to colony 1 comprising queen replacement, shook swarm method and Varroa control, whereas colony 2 was asphyxiated after queen loss and colony weakening. After therapy, colony 1 was wintered without symptoms. Beekeeping and sanitary measures can save a CBPV-infected colony, while further complications result in total colony loss.

For the first time, we analyzed the direct kinetic effects of concrete and rubber flooring on the soles of live dairy cows' claws while standing and walking. Ten adult dairy cows were equipped with foil-based pressure sensors (HoofSystem, Tekscan Inc., Boston, MA) under their left hind leg using a leather claw shoe. These sensors captured parameters of pressure distribution and vertical ground reaction forces while the cows walked on the 2 tested flooring types. The mean pressure was 15.1 to 21.1% lower on rubber flooring compared with concrete; mean pressure values (± standard deviation) were 36.32 ± 7.77 N/cm2 for static measures and 57.33 ± 11.77 N/cm2 for dynamic measures. We observed an even more obvious relief on rubber flooring in the maximum pressure loads, which were 30.1 to 32.7% lower on rubber flooring compared with concrete; mean pressure values (± standard deviation) were 98.79 ± 14.49 N/cm2 for static measures and 150.45 ± 20.87 N/cm2 for dynamic measures). The force-time curves of the dynamic measures essentially showed biphasic curve progression, with local peaks at 29 and 79% of the stance phase. However, we found considerable differences in curve progression between individuals and between the lateral and medial claws, which may be verified in further investigations with more animals. The study showed a noticeable reduction in mechanical load during standing and walking on rubber flooring compared with concrete.

Veterinarians are educated in prevention, diagnosis and treatment of diseases in various vertebrate species. As they are familiar with multifactorial health problems in single animals as well as in herd health management, their knowledge and skills can be beneficial for the beekeepers and honeybee health. However, in education and in practice, honeybees are not a common species for most veterinarians and the typical veterinary diagnostic methods such as blood sampling or auscultation are not applicable to the superorganism honeybee. Honeybee colonies may be affected by various biotic and abiotic factors. Among the infectious agents, RNA-viruses build the largest group, causing covert and overt infections in honeybee colonies which may lead to colony losses. Veterinarians could and should play a more substantial role in managing honeybee health—not limited to cases of notifiable diseases and official hygiene controls. This review discusses the veterinary diagnostic approach to adult bee examination with a special focus on diagnosis and differential diagnosis of the common virus diseases Acute Bee Paralysis Virus (ABPV)-Kashmir Bee Virus (KBV)-Israeli Acute Paralysis Virus (IAPV)-Complex, Chronic Bee Paralysis Virus (CBPV) and Deformed Wing Virus (DWV), as well as coinfections like Varroa spp. and Nosema spp.

Chalkbrood is a mycological brood disease of the Western honey bee (Apis mellifera), caused by the fungus Ascosphaera apis. The aim of this study was the investigation of the pathology of artificially reared Apis mellifera larvae, experimentally infected with A. apis spores (1.0 × 10³ spores/larva). Non-infected larvae served as control. Five living larvae and every dead larva were collected daily (day 1–7 p.i.). All larvae were macroscopically measured, photographed, formalin-fixed, and histologically processed (hematoxylin-eosin stain, Grocott silvering). Histological sections were digitized, and the size of the larvae was measured (mouth-after length, area) and statistically analyzed. Twenty-six larvae from the collected larvae (n = 64; 23 dead, 3 alive) showed histological signs of infection from 3 d p.i. onwards. The dead larvae showed macroscopically white/brown deposits, indistinct segmentation, and a lack of body elongation. Infected larvae were significantly smaller than the controls on days 3 p.i. (p < 0.05), 4 p.i. (p < 0.001), and 6 p.i. (p < 0.05). The early time of death, the low number of transitional stages, and the strong penetration of the larval carcass with fungal mycelium indicate a rapid and fulminant infection process, which is probably relevant for spreading the disease within the colony.

As part of the spine, the vertebral canal represents a central structure protecting the spinal cord running within it. Since alterations to the spinal canal and adjacent structures can have a significant impact on the spinal cord, knowledge of the physiological vertebral canal dimensions is essential. Compression of spinal nerves at the lumbosacral junction is the primary cause of cauda equina syndrome (CES). Although CES is common in dogs, it is rarely documented in cats. Given the lack of information on normal vertebral canal dimensions in cats, it is necessary to collect data and verify currently used measurements, to determine if and to what extent comparisons with dogs are valid. In 50 cats, interpedicular (ID) and midsagittal (SD) diameters were examined from the first thoracic (Th1) to the first sacral vertebra (S1). In 28 of these animals, the intervertebral disc width (IVDW) was measured. All data were gathered through gross anatomical dissection of the cats. Significant lumen reduction was evident in all cats from L6 to S1 with the narrowest point at S1. Narrowings were also found in the thoracic spine. The widest points coincide with the spinal cord enlargements. IVDW shows relatively constant values up to Th10–Th11 and peaks at L7–S1 in 95.65% of cats. While distinct similarities to dogs were observed, differences exist. The findings allow conclusions as to whether relations between the parameters and resulting predispositions to pathological changes can be derived. This could help the understanding of the pathogenesis of feline spinal diseases, particularly compressive myelopathies. Further studies are necessary to investigate the impact of age, sex and breed.

Mechanical overburdening is a major risk factor that provokes non-infectious claw diseases. Moreover, lameness-causing lesions often remain undetected and untreated. Therefore, prevention of claw tissue overburdening is of interest, especially by analyzing harmful effects within dairy cows’ housing environment. However, objective “on-cow” methods for bovine gait analysis are underdeveloped. The purpose of the study was to apply an innovative mobile pressure sensor system attached at the claws to perform pedobarometric gait analysis. A further goal was the supplementation with accelerative data, generated simultaneously by use of two inertial measurement units (IMUs), attached at metatarsal level. IMU data were analyzed with an automatic step detection algorithm. Gait analysis was performed in ten dairy cows, walking and trotting on concrete flooring and rubber mats. In addition to the basic applicability of the sensor systems and with the aid of the automatic step detection algorithm for gait analysis in cows, we were able to determine the impact of the gait and flooring type on kinematic and kinetic parameters. For pressure sensor output, concrete was associated with significantly (p < 0.001) higher maximum and average pressure values and a significantly smaller contact area, compared to rubber mats. In contrast to walking, trotting led to a significantly higher force, especially under the medial claw. Further, IMU-derived parameters were significantly influenced by the gait. The described sensor systems are useful tools for detailed gait analysis in dairy cows. They allow the investigation of factors which may affect claw health negatively.