Grassland species diversity monitoring is essential to grassland resource protection and utilization. “Spectral variation hypothesis” (SVH) provides a remote sensing method for monitoring grassland species diversity at pixel scale by calculating spectral heterogeneity. However, the pixel spectrum is easily affected by soil and other background factors in natural grassland. Unmanned aerial vehicle (UAV)-based imaging spectroscopy provides the possibility of soil information removal by virtue of its high spatial and spectral resolution. In this study, UAV-imaging spectroscopy data with a spatial resolution of 0.2 m obtained in two sites of typical alpine steppe within the Sanjiangyuan National Nature Reserve were used to analyze the relationships between four spectral diversity metrics (coefficient of variation based on NDVI (CV_NDVI), coefficient of variation based on multiple bands (CV_Multi), minimum convex hull volume (CHV) and minimum convex hull area (CHA)) and two species diversity indices (species richness and the Shannon–Wiener index). Meanwhile, two soil removal methods (based on NDVI threshold and the linear spectral unmixing model) were used to investigate the impact of soil on species diversity estimation. The results showed that the Shannon–Wiener index had a better response to spectral diversity than species richness, and CV_Multi showed the best correlation with the Shannon–Wiener index between the four spectral diversity metrics after removing soil information using the linear spectral unmixing model. It indicated that the estimation ability of spectral diversity to species diversity was significantly improved after removing the soil information. Our findings demonstrated the applicability of the spectral variation hypothesis in natural grassland, and illustrated the impact of soil on species diversity estimation.

The interactions between human activities and land cover have a significant impact on ecosystems. Therefore, studying human activity intensity based on land use or land cover is crucial for understanding the sustainable development of ecosystems. In this study, we selected Anhui Province as the study area and estimated the human activity intensity of land surface (HAILS) in 2015 and 2020 based on the ChinaCover datasets. We further analyzed the spatial, slope, and hydrological distribution characteristics of HAILS and explored the drivers of HAILS changes. The results show that the areas with higher HAILS were mainly located in the central part of Hefei, as well as along the Yangtze and the Huaihe rivers. The largest changes from 2015 to 2020 happened in the gentle slopes with the HAILS of 20–30%, and the percentage of HAILS > 20% decreased over the slope of 15°. In the riparian zone, the HAILS showed a clear decreasing trend after 2 km, while the HAILS in 2020 were higher than in 2015 in each flow-path distance belt, except for the Huaihe river. The HAILS index was strongly correlated with population density, rural population density, urban population density, average land GDP of primary industry, and nighttime light data. The rapid growth of the urban population and economy, as well as ecological protection policies, were identified as drivers of the above changes. Therefore, the HAILS in 2015 and 2020 of Anhui Province provide effective data support to address regional ecological conservation issues.

Forest aboveground biomass (AGB) is a key indicator for evaluating carbon sequestration capacity and forest productivity. Accurate regional-scale AGB estimation is crucial for advancing research on global climate change, ecosystem carbon cycles, and ecological conservation. Traditional methods, whether based on LiDAR or optical remote sensing, estimate AGB using planar density (t/ha) multiplied by pixel area, which fails to account for vertical forest structure variability. This study proposes a novel “stereoscopic (stereo) density × volume” approach, upgrading planar density to stereo density (t/ha/m) by integrating canopy height information, thereby improving estimation accuracy and exploring the feasibility of this new method. In the Daxing’anling region, plot-scale AGB estimation models were developed using stepwise linear regression (SLR) for both “planar density × area” and “stereo density × volume” methods. Results indicated that the stereo model using arithmetic mean height (H_AM) achieved comparable accuracy (R² = 0.83, RMSE = 2.77 t) with the planar model (R² = 0.83, RMSE = 2.52 t). At the regional scale, high-precision AGB estimates derived from airborne LiDAR were combined with vegetation indices from the Landsat Thematic Mapper (TM), and topographic factors from DEM to develop regional-scale AGB estimation models, using SLR and random forest (RF) algorithms. The results of 10-fold cross-validation demonstrated the superiority of the stereo method over the planar method, with RF outperforming SLR. The optimal RF-based stereo model of H_AM (R² = 0.65, rRMSE = 26.05%) significantly improved AGB estimation compared to the planar model (R² = 0.59, rRMSE = 30.41%). Independent accuracy validation using 75 field plots demonstrated that the stereo model achieved a higher validation R² of 0.45 compared to the planar model’s R² of 0.35. These findings suggest that the stereo approach mitigates the underestimation of AGB caused by forest height variability in planar methods, with no significant differences observed across forest types. In conclusion, the use of the stereo method to estimate forest AGB is superior to the planar method in optical remote sensing. This approach offers a scalable solution for forest AGB estimation and carbon stock assessment.

Accurate estimation of plantation aboveground biomass (AGB) is critical for quantifying carbon cycles and informing sustainable forest resource management, but enhancing estimation accuracy remains a key challenge. Although tree height and stand age are recognized as critical predictors for enhancing AGB models in addition to spectral vegetation indices, their individual and combined contributions in regional plantation forests remain insufficiently quantified, especially concerning the potential for leveraging the distinct characteristics of fast-growing plantations to facilitate AGB estimation. This study developed multi-source remote sensing-based Eucalyptus AGB estimation models for Nanning, Guangxi, integrating stand age and tree height to assess their impacts. Stand age was mapped from Landsat time-series imagery, and tree height was derived from UAV-LiDAR data. Plot-level reference AGB was obtained using fused UAV and terrestrial LiDAR point clouds. A random forest model, incorporating these variables with Sentinel-2 spectral information and topography, then achieved regional AGB estimation. The findings demonstrate that (1) tree height serves as the most influential predictor for AGB estimation at the regional scale, yielding a robust model performance (R² = 0.84). (2) Tree height captures the majority of the explanatory power associated with stand age. Once tree height was included as a predictor, the subsequent addition of stand age offered no significant improvement in model accuracy (R² = 0.85). (3) Given the challenges in obtaining precise tree height data and the robust correlation between stand age and tree height in fast-growing plantations, the integration of stand age substantially improved the accuracy of AGB estimations (from the spectral model of R² = 0.54 to R² = 0.74), with performance approaching that of tree height-based models (ΔR² = 0.10). Consequently, in fast-growing plantations, which are often characterized by high stand homogeneity, a hybrid model incorporating stand age can offer a reliable and cost-effective solution for AGB estimation.

Grassland aboveground biomass (AGB) is a key indicator of grassland ecosystem structure and function, and its accurate monitoring is of great importance for assessing grassland ecological conditions and supporting sustainable grassland management. Traditional biomass estimation methods based on vegetation indices (VIs) often suffer from saturation due to canopy shading. However, comparative studies on VI saturation and the saturation height of AGB detectable by different indices remain limited. In this study, we evaluated 12 commonly used VIs based on field-measured AGB and hyperspectral data in the Hulunbuir meadow steppe. Relationships between vertically accumulated biomass and VIs were analyzed to identify optimal AGB fitting models and to determine the saturation height of each index. Results showed that vertical distribution of AGB followed a unimodal pattern, with biomass peaking at approximately 36 cm in this region. This study employed four models (namely the Linear model, the Logarithmic model, the Power Function model and the Gompertz model) to fit the relationship between the vegetation index and AGB. Among them, Gompertz models consistently outperformed other models, indicating saturation across all indices. Based on saturation height, the 12 VIs were classified into two groups: ARVI, GNDVI, NDRE, OSAVI, and SAVI saturated at 40 cm, whereas DVI, EVI, MSAVI, NDPI, NDVI, RVI, and VARI maintained sensitivity up to 50 cm, demonstrating a stronger anti-saturation capacity. NDVI and NDPI exhibited the highest fitting accuracy and resistance to saturation. These findings validate the saturation limitations of VIs and provide guidance for selecting appropriate indices to improve the accuracy of grassland biomass retrieval.