Grassland species diversity monitoring is essential to grassland resource protection and utilization. “Spectral variation hypothesis” (SVH) provides a remote sensing method for monitoring grassland species diversity at pixel scale by calculating spectral heterogeneity. However, the pixel spectrum is easily affected by soil and other background factors in natural grassland. Unmanned aerial vehicle (UAV)-based imaging spectroscopy provides the possibility of soil information removal by virtue of its high spatial and spectral resolution. In this study, UAV-imaging spectroscopy data with a spatial resolution of 0.2 m obtained in two sites of typical alpine steppe within the Sanjiangyuan National Nature Reserve were used to analyze the relationships between four spectral diversity metrics (coefficient of variation based on NDVI (CV_NDVI), coefficient of variation based on multiple bands (CV_Multi), minimum convex hull volume (CHV) and minimum convex hull area (CHA)) and two species diversity indices (species richness and the Shannon–Wiener index). Meanwhile, two soil removal methods (based on NDVI threshold and the linear spectral unmixing model) were used to investigate the impact of soil on species diversity estimation. The results showed that the Shannon–Wiener index had a better response to spectral diversity than species richness, and CV_Multi showed the best correlation with the Shannon–Wiener index between the four spectral diversity metrics after removing soil information using the linear spectral unmixing model. It indicated that the estimation ability of spectral diversity to species diversity was significantly improved after removing the soil information. Our findings demonstrated the applicability of the spectral variation hypothesis in natural grassland, and illustrated the impact of soil on species diversity estimation.

Accurate estimations of forest total carbon storage are essential for understanding ecosystem functioning and improving forest management. This study investigates how multi-source remote sensing data can be used to provide accurate estimations of diameter at breast height (DBH) at the plot level, enhancing biomass estimations across 39.41 × 10⁴ km². The study is focused on Yunnan Province, China, which is characterized by complex terrain and diverse vegetation. Using ground-based survey data from hundreds of plots for model calibration and validation, the methodology combines multi-source remote sensing data, machine learning algorithms, and statistical analysis to develop models for estimating DBH distribution at regional scales. Decision tree showed the best overall performance. The model effectiveness improved when stratified by climatic zones, highlighting the importance of environmental context. Traditional methods based on the kNDVI index had a mean squared error (MSE) of 2575 t/ha and an R² value of 0.69. In contrast, combining model-estimated DBH values with remote sensing data resulted in a substantially lower MSE of 212 t/ha and a significantly improved R² value of 0.97. The results demonstrate that incorporating DBH not only reduced prediction errors but also improved the model’s ability to explain biomass variability. In addition, climatic region classification further increased model accuracy, suggesting that future efforts should consider environmental zoning. Our analyses indicate that water availability during cool and dry periods in this monsoon-influenced region was especially critical in influencing DBH across different subtropical zones. In summary, the study integrates DBH and high-resolution remote sensing data with advanced algorithms for accurate biomass estimation. The findings suggest that this approach can support regional forest management and contribute to research on carbon balance and ecosystem assessment.

Understanding the factors that drive distributional variation in terrestrial vegetation carbon pools (VCPs) is crucial for assessing the potential of carbon sequestration and mitigating climate change. Although previous studies have extensively examined elevational patterns of VCPs and their abiotic drivers, the biotic mechanisms that drive VCPs remain poorly understood, especially in savanna ecosystems. Characterized by a unique “tree-shrub-grass” plant composition, the savannas in the dry-hot valleys have high productivity and carbon storage capacity. In this study, we investigated VCP distribution and the factors that drive these distributions along an elevational gradient (400–1700 m) in the dry-hot valleys of Southwest China. We found no significant elevational pattern in the total VCP, whereas tree and shrub VCPs exhibited divergent distributional patterns. We also found that VCP distribution was more strongly affected by structural diversity than by species diversity, indicating that structural diversity mediates the relationship between species diversity and VCP distribution. These findings suggest that optimized spatial resource use enhances carbon storage. Notably, the total VCP was more influenced by tree structural diversity than by shrub structural diversity, highlighting the dominant role of trees in savanna carbon sequestration. Furthermore, elevation shaped the VCP patterns by regulating both soil and biotic factors. These findings provide valuable insights for carbon-oriented conservation and restoration practices in dryland ecosystems.

Ecotones, i.e., transition zones between habitats, are important landscape features, yet they are often ignored in landscape monitoring. This study addresses the challenge of delineating ecotones at multiple scales by integrating multisource remote sensing data, including ultra-high-resolution RGB images, LiDAR data from UAVs, and satellite data. We first developed a fine-resolution landcover map of three plots in Yunnan, China, with accurate delineation of ecotones using orthoimages and canopy height data derived from UAV-LiDAR. These maps were subsequently used as the training set for four machine learning models, from which the most effective model was selected as an upscaling model. The satellite data, encompassing Synthetic Aperture Radar (SAR; Sentinel-1), multispectral imagery (Sentinel-2), and topographic data, functioned as explanatory variables. The Random Forest model performed the best among the four models (kappa coefficient = 0.78), with the red band, shortwave infrared band, and vegetation red edge band as the most significant spectral variables. Using this RF model, we compared landscape patterns between 2017 and 2023 to test the model’s ability to quantify ecotone dynamics. We found an increase in ecotone over this period that can be attributed to an expansion of 0.287 km² (1.1%). In sum, this study demonstrates the effectiveness of combining UAV and satellite data for precise, large-scale ecotone detection. This can enhance our understanding of the dynamic relationship between ecological processes and landscape pattern evolution.