The extraction process of crude polysaccharides from Glycyrrhiza (GP) by ultrasonic assisted hot water method was established according to the optimized kinetic model based on Fick's second law of diffusion, and thermodynamic action was analyzed. Physicochemical properties of GP including the apparent viscosity, thermostability and antioxidant activities etc. were determined by ultraviolet spectrophotometry and differential scanning calorimetry (DSC). Characteristic function groups and surface structure also were analyzed by Fourier infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and atomic force microscopy (AFM), respectively. The results showed the extraction kinetic curves of GP showed good linear correlation with the linear correlation coefficients (R2) of equal or greater than 0.90 based on Fick's second law of diffusion, and the maximum yield of 3.53% was obtained at 343.15 K and ultrasonic power 600 W with material-liquid ratio of 1:15 for 60 min. Gibbs free energy change (ΔGm > 0) indicated that the extraction process was endergonic and not spontaneous. GP was confirmed a kind of acidic pyran polysaccharide with small bubble-like holes internally. The GP viscosity increased with the increase of concentration and then gradually decreased with the enhancement of shear rate. GP showed good thermal stability along with two stages of mass loss by DSC analysis. The antioxidant activity experiments suggested that the higher the concentration of GP, the stronger its reduction power.

The northern Tibetan Plateau is the most traditional and important semi-nomadic region in Tibet. The alpine vegetation is sensitive and vulnerable to climate change and human activities, and is also important as an ecological security in protecting the headwaters of major rivers in Asia. Therefore, the Tibetan alpine grasslands have fundamental significance to both Mainland China and South Asia. The pasture degradation, however, likely threatens the livelihood of residents and the habitats of wildlife on this plateau. Since 2004, the government has launched a series of ecological restoration projects and economic compensatory payment polices. Many fences were additionally built on degraded pastures to prevent new degradation, to promote functionality recovery, and to balance the stocking rate with forage productivity. The grazed vs. fenced paired pastures across different zonal grassland communities along evident environmental gradients provide us with a natural comparative experiment platform to test the relative contributions of natural and anthropogenic factors. This study critically reviews the background, significance of and debates on short-term grazing exclusion with fences in this region. We also aim to figure out scientific and standardized workflows for assessing the effectiveness of grazing exclusion and compensatory payments in the future.

Quantifying the impact of climate change and vegetation phenology on ecosystem productivity in the alpine grasslands of the Tibetan Plateau (TP) is essential for assessing carbon balance dynamics at regional and global scales. However, the relative contributions of climate change and phenological change to vegetation productivity across various grassland types remain indistinguishable. This study examined the effects of climate change and phenological change on net primary productivity (NPP) in the alpine meadow and alpine steppe ecosystems of the TP from 2001 to 2020. The results revealed that (1) NPP exhibited a positive correlation with vegetation phenology, particularly with an extended growing season length and an earlier start of the growing season. Among the phenological variables studied, changes in the start of the growing season had the strongest influence on NPP variations in both alpine meadows and alpine steppes. (2) NPP displayed a positive correlation with annual precipitation and annual temperature, with changes in annual precipitation playing a dominant role in shaping NPP variations in alpine steppes. (3) NPP showed a negative correlation with annual radiation, and the impact of radiation changes on NPP variations was comparable to that of precipitation or temperature in both alpine meadows and alpine steppes. (4) Climate change exerted a stronger impact on NPP than phenological change in alpine steppes, while NPP was jointly affected by climate change and phenological change in alpine meadows. Our findings indicated that the relative effects of climate change and phenological change on vegetation productivity vary across different grassland types on the TP.

In order to explore responses of soil microbial communities among different alpine meadows under warming and clipping, soil microorganisms of three alpine meadow sites (low altitude: 4313 m, alpine steppe meadow, 30°30′ N, 91°04′ E; mid-altitude: 4513 m, alpine steppe meadow, 30°31′ N, 91°04′ E; and high altitude: 4693, alpine Kobresia meadow, 30°32′ N, 91°03′ E) were measured using the phospholipid fatty acid (PLFA) method. Both warming and clipping significantly reduced PLFA content and changed the community composition of soil microbial taxa, which belong to bacterial and fungal communities in the alpine Kobresia meadow. Warming significantly reduced the soil total PLFA content by 36.1% and the content of soil fungi by 37.0%; the clipping significantly reduced the soil total PLFA content by 57.4%, the content of soil fungi by 49.9%, and the content of soil bacteria by 60.5% in the alpine Kobresia meadow. Only clipping changed the total fungal community composition at a low altitude. Neither clipping nor warming changed the microbial community composition at a moderate altitude. Soil temperature, soil moisture, and pH were the main factors affecting soil microbial communities. Therefore, the effects of warming and clipping on soil microbial communities in alpine meadows were related to grassland types and soil environmental conditions.