Abiotic stresses, such as heat, salt, waterlogging, and multiple-stress environments have significantly reduced wheat production in recent decades. There is a need to use effective strategies for overcoming crop losses due to these abiotic stresses. Fertilizer-based approaches are readily available and can be managed in all farming communities. This research revealed the effects of sulfur-coated urea (SCU, 130 kg ha⁻¹, release time of 120 days) on wheat crops under heat, salt, waterlogging, and combined-stress climatic conditions. The research was done using a completely randomized design with three replicates. The results revealed that SCU at a rate of 130 kg of N ha⁻¹ showed a significantly (p ≤ 0.05) high SPAD value (55) in the case of waterlogging stress, while it was the lowest (31) in the case of heat stress; the control had a SPAD value of 58. Stress application significantly (p ≤ 0.05) reduced the leaf area and was the highest in control (1898 cm²), followed by salt stress (1509 cm²), waterlogging (1478 cm²), and heat stress (1298 cm²). A significantly (p ≤ 0.05) lowest crop yield was observed in the case of heat stress (3623.47 kg ha⁻¹) among all stresses, while it was 10,270 kg ha⁻¹ in control and was reduced up to 35% after the application of heat stress. Among all stresses, the salt stress showed the highest crop yield of 5473.16 kg ha⁻¹. A significant correlation was observed among growth rate, spike length, yield, and physiological constraints with N content in the soil. The SCU fertilizer was the least effective against heat stress but could tolerate salt stress in wheat plants. The findings suggested the feasibility of adding SCU as an alternative to normal urea to alleviate salt stresses and improve wheat crop growth and yield traits. For heat stress tolerance, the applicability of SCU with a longer release period of ~180 days is recommended as a future prospect for study.

Nitrogen (N) is an essential nutrient element for crop productivity. Unfortunately, the nitrogen use efficiency (NUE) of crop plants gradually decreases with the increase of the N application rate. Nevertheless, little has been known about the molecular mechanisms of differences in NUE among genotypes of wheat. In this study, we used RNA-Sequencing (RNA-Seq) to compare the transcriptome profiling of flag leaves at the stage of anthesis in wheat NILs (1Y, high-NUE, and 1W, low-NUE) under normal nitrogen conditions (300 kg N ha⁻¹, corresponding to 1.6 g N pot⁻¹). We identified 7023 DEGs (4738 upregulated and 2285 downregulated) in the comparison between lines 1Y and 1W. The responses of 1Y and 1W to normal N differed in the transcriptional regulatory mechanisms. Several genes belonging to the GS and GOGAT gene families were upregulated in 1Y compared with 1W, and the enhanced carbon metabolism might lead 1Y to produce more C skeletons, metabolic energy, and reductants for nitrogen metabolism. A subset of transcription factors (TFs) family members, such as ERF, WRKY, NAC, and MYB, were also identified. Collectively, these identified candidate genes provided new information for a further understanding of the genotypic difference in NUE.

Environmental conditions (precipitation, temperature and soil properties) differ greatly in different regions and have dual effects on the wheat growth and nutrient release of slow-release fertilizer (SRF). Conventional fertilization methods such as the multiple-split application of urea and the one-time application of SRF may have difficulty achieving a stable and high wheat yield and nitrogen (N) efficiency in various environments. Therefore, the exploration of a rational application strategy of SRF is needed for improving wheat yield and its stability in different regions. A two-year field experiment was conducted in different regions (eight test sites per year) with five patterns: 100% N (270 kg ha⁻¹) SRF applied pre-sowing (M1); 60% N SRF applied pre-sowing and 40% N urea applied at jointing (M2); 60% N SRF applied pre-sowing and 40% N SRF applied at re-greening (M3); M2 reducing the N rate by 15% (M4); M3 reducing the N rate by 15% (M5). The fourth-split application of urea was taken as the control (CK, 270 kg N ha⁻¹). The results suggested that the average yield in M1 decreased by 3.65% of the CK, and the yield stability was poor. Both M2 and M3 significantly increased N efficiency, grain yield and benefit, but the stability of M3 was higher than that of M2 in different environments. Considering further improvements in wheat yield, N efficiency and profit, our results suggested that the twice-split application of SRF, which also improved the adaptability of wheat in different environments, could be recommended for wheat cultivation.

The development of crop cultivars with high nitrogen use efficiency (NUE) under low-N fertilizer inputs is imperative for sustainable agriculture. However, there has been little research on the molecular mechanisms underlying enhanced resilience to low N in high-NUE plants. The comparison of the transcriptional responses of genotypes contrasting for NUE will facilitate an understanding of the key molecular mechanism of wheat resilience to low-N stress. In the current study, the RNA sequencing (RNA-seq) technique was employed to investigate the genotypic difference in response to N deficiency between two wheat NILs (1Y, high-NUE, and 1W, low-NUE). In our research, high- and low-NUE wheat NILs showed different patterns of gene expression under N-deficient conditions, and these N-responsive genes were classified into two major classes, including “frontloaded genes” and “relatively upregulated genes”. In total, 103 and 45 genes were identified as frontloaded genes in high-NUE and low-NUE wheat, respectively. In summary, our study might provide potential directions for further understanding the molecular mechanism of high-NUE genotypes adapting to low-N stress.

Optimizing soil properties to match ecological conditions can alleviate stress damage and promote crop growth. However, the suitable soil conditions for wheat growth in an integrated rice–wheat breeding scheme under high rainfall and the mechanisms that affect yield production are not well known. Field experiments were carried out at two sites, which were all located in Jiangsu Province, China, a subtropical monsoon climate zone during two wheat growing seasons, to assess the effects of plow tillage followed by rotary tillage (PR) and no-tillage (NT) on soil physical and chemical properties, wheat seedling growth, grain yield, and spike amounts and quality. The finding indicates that with the reduction in soil mixing, soil bulk density was higher in NT than in PR, which helped to maintain moisture in dry soil. In soils with high water content, in NT, when the wheat field was subjected to waterlogging stress, the drainage decreased to deeper soil possibly due to reduced infiltration and a higher evaporation of surface water. The diurnal variation in soil temperature decreased in NT, and when the soil was cold, NT helped to insulate soils at 0–25 cm. Compared with PR, the contents of available nitrogen and phosphorus increased at 0–20 cm in NT. Root biomass and root activity of wheat seedlings at 0–20 cm were also greater in NT than in PR. Compared with PR, wheat also had more culms at the beginning of the overwintering stage, more spikes, and higher grain yield in NT, but the differences were not significant under excessive soil moisture. Therefore, the soil hydrothermal environment and spatial distributions of nutrients in NT promoted shallow root growth and tiller development in the early phase of wheat growth, which led to higher amounts of spikes per plant that resulted in high-yielding wheat crops.