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Plant endophytes are known to alter the profile of secondary metabolites in plant hosts. In this study, we identified the main bacterial and fungal representatives of the wild grape Vitis amurensis Rupr. microbiome and investigated a cocultivation effect of the 14 endophytes and the V. amurensis cell suspension on biomass accumulation and stilbene biosynthesis. The cocultivation of the V. amurensis cell culture with the bacteria Agrobacterium sp., Bacillus sp., and Curtobacterium sp. for 2 weeks did not significantly affect the accumulation of cell culture fresh biomass. However, it was significantly inhibited by the bacteria Erwinia sp., Pantoea sp., Pseudomonas sp., and Xanthomonas sp. and fungi Alternaria sp., Biscogniauxia sp., Cladosporium sp., Didymella sp. 2, and Fusarium sp. Cocultivation of the grapevine cell suspension with the fungi Didymella sp. 1 and Trichoderma sp. resulted in cell death. The addition of endophytic bacteria increased the total stilbene content by 2.2–5.3 times, while the addition of endophytic fungi was more effective in inducing stilbene accumulation by 2.6–16.3 times. The highest content of stilbenes in the grapevine cells cocultured with endophytic fungi was 13.63 and 13.76 mg/g of the cell dry weight (DW) after cultivation with Biscogniauxia sp. and Didymella sp. 2, respectively. The highest content of stilbenes in the grapevine cells cocultured with endophytic bacteria was 4.49 mg/g DW after cultivation with Xanthomonas sp. The increase in stilbene production was due to a significant activation of phenylalanine ammonia lyase (PAL) and stilbene synthase (STS) gene expression. We also analyzed the sensitivity of the selected endophytes to eight antibiotics, fluconazole, and trans-resveratrol. The endophytic bacteria were sensitive to gentamicin and kanamycin, while all selected fungal strains were resistant to fluconazole with the exception of Cladosporium sp. All endophytes were tolerant of trans-resveratrol. This study showed that grape endophytes stimulate the production of stilbenes in grape cell suspension, which could further contribute to the generation of a new stimulator of stilbene biosynthesis in grapevine or grape cell cultures.
Olga Aleynova; Andrey Suprun; Nikolay Nityagovsky; Alexandra Dubrovina; Konstantin Kiselev. The Influence of the Grapevine Bacterial and Fungal Endophytes on Biomass Accumulation and Stilbene Production by the In Vitro Cultivated Cells of Vitis amurensis Rupr. Plants 2021, 10, 1276 .
AMA StyleOlga Aleynova, Andrey Suprun, Nikolay Nityagovsky, Alexandra Dubrovina, Konstantin Kiselev. The Influence of the Grapevine Bacterial and Fungal Endophytes on Biomass Accumulation and Stilbene Production by the In Vitro Cultivated Cells of Vitis amurensis Rupr. Plants. 2021; 10 (7):1276.
Chicago/Turabian StyleOlga Aleynova; Andrey Suprun; Nikolay Nityagovsky; Alexandra Dubrovina; Konstantin Kiselev. 2021. "The Influence of the Grapevine Bacterial and Fungal Endophytes on Biomass Accumulation and Stilbene Production by the In Vitro Cultivated Cells of Vitis amurensis Rupr." Plants 10, no. 7: 1276.
Exogenous application of double-stranded RNAs (dsRNAs) and small-interfering RNAs (siRNAs) to plant surfaces has emerged as a promising method for regulation of essential genes in plant pathogens and for plant disease protection. Yet, regulation of plant endogenous genes via external RNA treatments has not been sufficiently investigated. In this study, we targeted the genes of chalcone synthase (CHS), the key enzyme in the flavonoid/anthocyanin biosynthesis pathway, and two transcriptional factors, MYBL2 and ANAC032, negatively regulating anthocyanin biosynthesis in Arabidopsis. Direct foliar application of AtCHS-specific dsRNAs and siRNAs resulted in an efficient downregulation of the AtCHS gene and suppressed anthocyanin accumulation in A. thaliana under anthocyanin biosynthesis-modulating conditions. Targeting the AtMYBL2 and AtANAC032 genes by foliar dsRNA treatments markedly reduced their mRNA levels and led to a pronounced upregulation of the AtCHS gene. The content of anthocyanins was increased after treatment with AtMYBL2-dsRNA. Laser scanning microscopy showed a passage of Cy3-labeled AtCHS-dsRNA into the A. thaliana leaf vessels, leaf parenchyma cells, and stomata, indicating the dsRNA uptake and spreading into leaf tissues and plant individual cells. Together, these data show that exogenous dsRNAs were capable of downregulating Arabidopsis genes and induced relevant biochemical changes, which may have applications in plant biotechnology and gene functional studies.
Konstantin Kiselev; Andrey Suprun; Olga Aleynova; Zlata Ogneva; Alexander Kalachev; Alexandra Dubrovina. External dsRNA Downregulates Anthocyanin Biosynthesis-Related Genes and Affects Anthocyanin Accumulation in Arabidopsis thaliana. International Journal of Molecular Sciences 2021, 22, 6749 .
AMA StyleKonstantin Kiselev, Andrey Suprun, Olga Aleynova, Zlata Ogneva, Alexander Kalachev, Alexandra Dubrovina. External dsRNA Downregulates Anthocyanin Biosynthesis-Related Genes and Affects Anthocyanin Accumulation in Arabidopsis thaliana. International Journal of Molecular Sciences. 2021; 22 (13):6749.
Chicago/Turabian StyleKonstantin Kiselev; Andrey Suprun; Olga Aleynova; Zlata Ogneva; Alexander Kalachev; Alexandra Dubrovina. 2021. "External dsRNA Downregulates Anthocyanin Biosynthesis-Related Genes and Affects Anthocyanin Accumulation in Arabidopsis thaliana." International Journal of Molecular Sciences 22, no. 13: 6749.
: Recent studies have revealed that foliar application of double-stranded RNAs (dsRNAs) or small-interfering RNAs (siRNAs) encoding specific genes of plant pathogens triggered RNA interference (RNAi)-mediated silencing of the gene targets. However, a limited number of reports documented silencing of plant endogenes or transgenes after direct foliar RNA application. This study analyzed the importance of physiological conditions (plant age, time of day, soil moisture, high salinity, heat, and cold stresses) and different dsRNA application means (brush spreading, spraying, infiltration, inoculation, needle injection, and pipetting) for suppression of neomycin phosphotransferase II (NPTII) transgene in Arabidopsis thaliana, as transgenes are more prone to silencing. We observed a higher NPTII suppression when dsRNA was applied at late day period, being most efficient at night, which revealed a diurnal variation in dsRNA treatment efficacy. Exogenous NPTII-dsRNA considerably reduced NPTII expression in 4-week-old plants and only limited it in 2- and 6-week-old plants. In addition, a more discernible NPTII downregulation was detected under low soil moisture conditions. Treatment of adaxial and abaxial leaf surfaces by brushes, spraying, and pipetting showed a higher NPTII suppression, while infiltration and inoculation were less efficient. Thus, appropriate plant age, late time of day, low soil moisture, and optimal dsRNA application modes are important for exogenously induced gene silencing.
Konstantin V. Kiselev; Andrey R. Suprun; Olga A. Aleynova; Zlata V. Ogneva; Alexandra S. Dubrovina. Physiological Conditions and dsRNA Application Approaches for Exogenously induced RNA Interference in Arabidopsis thaliana. Plants 2021, 10, 264 .
AMA StyleKonstantin V. Kiselev, Andrey R. Suprun, Olga A. Aleynova, Zlata V. Ogneva, Alexandra S. Dubrovina. Physiological Conditions and dsRNA Application Approaches for Exogenously induced RNA Interference in Arabidopsis thaliana. Plants. 2021; 10 (2):264.
Chicago/Turabian StyleKonstantin V. Kiselev; Andrey R. Suprun; Olga A. Aleynova; Zlata V. Ogneva; Alexandra S. Dubrovina. 2021. "Physiological Conditions and dsRNA Application Approaches for Exogenously induced RNA Interference in Arabidopsis thaliana." Plants 10, no. 2: 264.
A Calothrix-like cyanobacterial strain was isolated from a sample collected from the entrance zone of Shumyaschiy Ponor cave, Chelyabinsk Region, South Urals, Russia. This isolate showed diagnostic features of the recently described genus Roholtiella, i.e. chains of arthrospores and thalli not tapering to a hair. Molecular analyses based on 16S rRNA gene, 16S–23S internal transcribed spacer (ITS) sequences, and spacer secondary structure revealed that our isolate was a distinct lineage within the genus clade that we describe as Roholtiella mixta sp. nov. High-performance liquid chromatography identified β-carotene, zeaxanthin, 3-hydroxyechinenone (4-keto-3-hydroxy-β-carotene), echinenone, caloxanthin, β-cryptoxanthinoleate, and antheraxanthin in R. mixta. Antheraxanthin was recorded for the second time only in cyanobacteria species.
Shamil R. Abdullin; Vyacheslav Yu. Nikulin; Arthur Yu. Nikulin; Artem Yu. Manyakhin; Veronika B. Bagmet; Andrey R. Suprun; Andrey A. Gontcharov. Roholtiella mixta sp. nov. (Nostocales, Cyanobacteria): morphology, molecular phylogeny, and carotenoid content. Phycologia 2021, 60, 73 -82.
AMA StyleShamil R. Abdullin, Vyacheslav Yu. Nikulin, Arthur Yu. Nikulin, Artem Yu. Manyakhin, Veronika B. Bagmet, Andrey R. Suprun, Andrey A. Gontcharov. Roholtiella mixta sp. nov. (Nostocales, Cyanobacteria): morphology, molecular phylogeny, and carotenoid content. Phycologia. 2021; 60 (1):73-82.
Chicago/Turabian StyleShamil R. Abdullin; Vyacheslav Yu. Nikulin; Arthur Yu. Nikulin; Artem Yu. Manyakhin; Veronika B. Bagmet; Andrey R. Suprun; Andrey A. Gontcharov. 2021. "Roholtiella mixta sp. nov. (Nostocales, Cyanobacteria): morphology, molecular phylogeny, and carotenoid content." Phycologia 60, no. 1: 73-82.
Recent research has shown that plants can uptake long dsRNAs and dsRNA-derived siRNAs that target important genes of infecting fungi or viruses when applied on the surface of plant leaves. The external RNAs were capable of local and systemic movement inducing plant resistance against the pathogens. Few studies have been made for plant gene regulation by foliar application of RNAs. In this study, several types of ssRNA and siRNA duplexes targeting the neomycin phosphotransferase II (NPTII) transgene were in vitro-synthesized and externally applied to the leaf surface of 4-week-old transgenic Arabidopsis thaliana plants. External application of the synthetic NPTII-encoding siRNAs down-regulated NPTII transcript levels in transgenic A. thaliana 1 and 7 days post-treatment with a higher and more consistent effect being observed for siRNAs methylated at 3' ends. We also analyzed the effects of external NPTII-encoding dsRNA precursors and a dsRNA-derived heterogenous siRNA mix. Digestion of the NPTII-dsRNA to the heterogeneous siRNAs did not improve efficiency of the transgene suppression effect. Key Points• Foliar application of siRNAs down-regulated a commonly used transgene in Arabidopsis. • A more consistent effect was observed for methylated siRNAs. • The findings are important for development of plant gene regulation approaches.
Alexandra S. Dubrovina; Olga A. Aleynova; Andrey Suprun; Zlata V. Ogneva; Konstantin V. Kiselev. Transgene suppression in plants by foliar application of in vitro-synthesized small interfering RNAs. Applied Microbiology and Biotechnology 2020, 104, 2125 -2135.
AMA StyleAlexandra S. Dubrovina, Olga A. Aleynova, Andrey Suprun, Zlata V. Ogneva, Konstantin V. Kiselev. Transgene suppression in plants by foliar application of in vitro-synthesized small interfering RNAs. Applied Microbiology and Biotechnology. 2020; 104 (5):2125-2135.
Chicago/Turabian StyleAlexandra S. Dubrovina; Olga A. Aleynova; Andrey Suprun; Zlata V. Ogneva; Konstantin V. Kiselev. 2020. "Transgene suppression in plants by foliar application of in vitro-synthesized small interfering RNAs." Applied Microbiology and Biotechnology 104, no. 5: 2125-2135.
Plant calmodulins (CaMs) and calmodulin-like proteins (CMLs) are important plant Ca2+-binding proteins that sense and decode changes in the intracellular Ca2+ concentration arising in response to environmental stimuli. Protein Ca2+ sensors are presented by complex gene families in plants and perform diverse biological functions. In this study, we cloned, sequenced, and characterized three CaM and 54 CML mRNA transcripts of Vitis amurensis Rupr., a wild-growing grapevine with a remarkable stress tolerance. Using real-time quantitative RT-PCR, we analyzed transcript abundance of the identified VaCaMs and VaCMLs in response to water deficit, high salinity, high mannitol, cold and heat stresses. Expression of VaCaMs and 32 VaCMLs actively responded to the abiotic stresses and exhibited both positive and negative regulation patterns. Other VaCML members showed slight transcriptional regulation, remained essentially unresponsive or responded only after one time interval of the treatments. The substantial alterations in the VaCaM and VaCML transcript levels revealed their involvement in the adaptation of wild-growing grapevine to environmental stresses.
Alexandra S. Dubrovina; Olga A. Aleynova; Zlata V. Ogneva; Andrey R. Suprun; Alexey A. Ananev; Konstantin V. Kiselev. The Effect of Abiotic Stress Conditions on Expression of Calmodulin (CaM) and Calmodulin-Like (CML) Genes in Wild-Growing Grapevine Vitis amurensis. Plants 2019, 8, 602 .
AMA StyleAlexandra S. Dubrovina, Olga A. Aleynova, Zlata V. Ogneva, Andrey R. Suprun, Alexey A. Ananev, Konstantin V. Kiselev. The Effect of Abiotic Stress Conditions on Expression of Calmodulin (CaM) and Calmodulin-Like (CML) Genes in Wild-Growing Grapevine Vitis amurensis. Plants. 2019; 8 (12):602.
Chicago/Turabian StyleAlexandra S. Dubrovina; Olga A. Aleynova; Zlata V. Ogneva; Andrey R. Suprun; Alexey A. Ananev; Konstantin V. Kiselev. 2019. "The Effect of Abiotic Stress Conditions on Expression of Calmodulin (CaM) and Calmodulin-Like (CML) Genes in Wild-Growing Grapevine Vitis amurensis." Plants 8, no. 12: 602.
Stilbenes are natural compounds protecting plants against microbial pathogens and known to possess valuable biologically active properties. In the present study, we established transgenic grapevine callus cell cultures overexpressing three stilbene synthase (STS) genes of spruce Picea jezoensis PjSTS1a, PjSTS2, and PjSTS3. Transformation of Vitis amurensis calli with the PjSTS1a, PjSTS2, and PjSTS3 genes significantly increased total content of stilbenes in 3.6-6, 2.5-2.9, and 4.1-16.1 times, respectively, in comparison with the control calli. The most pronounced positive effect on the accumulation of stilbenes was observed for the PjSTS3-overexpressing calli where the total content of stilbenes was increased up to 3.1 mg/g DW, and the stilbene production reached 25.4 mg/L. These values were higher than those achieved for the grapevine callus cell cultures overexpressing three STS genes from V. amurensis. Thus, transformation of grapevine cell cultures with spruce STS genes with a relatively low degree of homology to the endogenous VaSTSs is a more effective strategy for induction of plant secondary metabolite biosynthesis than using the grapevine genes for the overexpression experiments.
Andrey Suprun; Z. V. Ogneva; A. S. Dubrovina; K. V. Kiselev. Effect of spruce PjSTS1a , PjSTS2 , or PjSTS3 gene overexpression on stilbene biosynthesis in callus cultures of Vitis amurensis Rupr. Biotechnology and Applied Biochemistry 2019, 67, 234 -239.
AMA StyleAndrey Suprun, Z. V. Ogneva, A. S. Dubrovina, K. V. Kiselev. Effect of spruce PjSTS1a , PjSTS2 , or PjSTS3 gene overexpression on stilbene biosynthesis in callus cultures of Vitis amurensis Rupr. Biotechnology and Applied Biochemistry. 2019; 67 (2):234-239.
Chicago/Turabian StyleAndrey Suprun; Z. V. Ogneva; A. S. Dubrovina; K. V. Kiselev. 2019. "Effect of spruce PjSTS1a , PjSTS2 , or PjSTS3 gene overexpression on stilbene biosynthesis in callus cultures of Vitis amurensis Rupr." Biotechnology and Applied Biochemistry 67, no. 2: 234-239.
Recent investigations show that exogenously applied small interfering RNAs (siRNA) and long double-stranded RNA (dsRNA) precursors can be taken up and translocated in plants to induce RNA interference (RNAi) in the plant or in its fungal pathogen. The question of whether genes in the plant genome can undergo suppression as a result of exogenous RNA application on plant surface is almost unexplored. This study analyzed whether it is possible to influence transcript levels of transgenes, as more prone sequences to silencing, in Arabidopsis genome by direct exogenous application of target long dsRNAs. The data revealed that in vitro synthesized dsRNAs designed to target the gene coding regions of enhanced green fluorescent protein (EGFP) or neomycin phosphotransferase II (NPTII) suppressed their transcript levels in Arabidopsis. The fact that, simple exogenous application of polynucleotides can affect mRNA levels of plant transgenes, opens new opportunities for the development of new scientific techniques and crop improvement strategies.
Alexandra S. Dubrovina; Olga A. Aleynova; Alexander V. Kalachev; Andrey Suprun; Zlata V. Ogneva; Konstantin V. Kiselev. Induction of Transgene Suppression in Plants via External Application of Synthetic dsRNA. International Journal of Molecular Sciences 2019, 20, 1585 .
AMA StyleAlexandra S. Dubrovina, Olga A. Aleynova, Alexander V. Kalachev, Andrey Suprun, Zlata V. Ogneva, Konstantin V. Kiselev. Induction of Transgene Suppression in Plants via External Application of Synthetic dsRNA. International Journal of Molecular Sciences. 2019; 20 (7):1585.
Chicago/Turabian StyleAlexandra S. Dubrovina; Olga A. Aleynova; Alexander V. Kalachev; Andrey Suprun; Zlata V. Ogneva; Konstantin V. Kiselev. 2019. "Induction of Transgene Suppression in Plants via External Application of Synthetic dsRNA." International Journal of Molecular Sciences 20, no. 7: 1585.
The effect of 5-azacytidine (5A)-induced DNA hypomethylation on the growth and abiotic stress tolerance of Arabidopsis thaliana were analysed. Growth analysis revealed that aqueous solutions of 5A added to the soil did not affect the fresh and dry biomass accumulation but led to a higher percentage of flowering A. thaliana plants after four weeks of cultivation. The 5A treatment considerably lowered survival rates of Arabidopsis plants under high soil salinity, heat stress, and drought, while it did not affect the survival rates after freezing stress. 5A eliminated the stimulatory effect of the heat and drought stresses on the transcriptional levels of a number of stress-inducible genes, such as DREB1, LEA, SOS1, or RD29A. A less clear but similar trend has been detected for the effect of 5A on expression of the stress-inducible genes under salt and cold stresses. The data indicate that DNA methylation is an important mechanism regulating plant abiotic stress resistance.
Zlata V. Ogneva; Andrey Suprun; Alexandra S. Dubrovina; Konstantin V. Kiselev. Effect of 5-azacytidine induced DNA demethylation on abiotic stress tolerance in Arabidopsis thaliana. Plant Protection Science 2019, 55, 73 -80.
AMA StyleZlata V. Ogneva, Andrey Suprun, Alexandra S. Dubrovina, Konstantin V. Kiselev. Effect of 5-azacytidine induced DNA demethylation on abiotic stress tolerance in Arabidopsis thaliana. Plant Protection Science. 2019; 55 (No. 2):73-80.
Chicago/Turabian StyleZlata V. Ogneva; Andrey Suprun; Alexandra S. Dubrovina; Konstantin V. Kiselev. 2019. "Effect of 5-azacytidine induced DNA demethylation on abiotic stress tolerance in Arabidopsis thaliana." Plant Protection Science 55, no. No. 2: 73-80.
This study examines the effects of p-coumaric (CoA) and caffeic (CaA) acids on stilbene biosynthesis in one-year-old needles of Picea jezoensis (Lindl. et Gord.) Fisch ex Carr. under control conditions and after ultraviolet (UV-C) irradiation. HPLC analysis revealed that while UV-C irradiation slightly affected the total stilbene content, CoA and CaA feeding increased the total content of stilbenes by 1.2-1.3-fold. UV-C treatment combined with CoA-feeding of the P. jezoensis cuttings exerted the most pronounced stimulatory effect on stilbene accumulation leading to the total stilbene content of 9.18 mg/g of dry weight or DW. This increase correlated with the elevated transcription of the stilbene synthase PjSTS1a and PjSTS1b genes. UV-C treatment in combination with CaA feeding of P. jezoensis did not considerably influence stilbene content. These results revealed a positive effect of UV-C radiation and phenolic precursors on the content of stilbenes in spruce needles.
K.V. Kiselev; V.P. Grigorchuk; Z.V. Ogneva; Andrey Suprun; A.S. Dubrovina. The effect of ultraviolet-C and precursor feeding on stilbene biosynthesis in spruce Picea jezoensis. Journal of Plant Physiology 2019, 234-235, 133 -137.
AMA StyleK.V. Kiselev, V.P. Grigorchuk, Z.V. Ogneva, Andrey Suprun, A.S. Dubrovina. The effect of ultraviolet-C and precursor feeding on stilbene biosynthesis in spruce Picea jezoensis. Journal of Plant Physiology. 2019; 234-235 ():133-137.
Chicago/Turabian StyleK.V. Kiselev; V.P. Grigorchuk; Z.V. Ogneva; Andrey Suprun; A.S. Dubrovina. 2019. "The effect of ultraviolet-C and precursor feeding on stilbene biosynthesis in spruce Picea jezoensis." Journal of Plant Physiology 234-235, no. : 133-137.
An inhibitor of DNA methylation 5-azacytidine (5A) is a chemical analog of the nucleoside cytidine. This study investigated the influence of 5A-induced DNA hypomethylation on the accumulation of somatic DNA mutations (nucleotide substitutions,...
K.V. Kiselev; Z.V. Ogneva; A.S. Dubrovina; N.N. Nityagovsky; Andrey Suprun. Somatic mutations, DNA methylation, and expression of DNA repair genes in Arabidopsis thaliana treated with 5-azacytidine. Biologia plantarum 2019, 63, 398 -404.
AMA StyleK.V. Kiselev, Z.V. Ogneva, A.S. Dubrovina, N.N. Nityagovsky, Andrey Suprun. Somatic mutations, DNA methylation, and expression of DNA repair genes in Arabidopsis thaliana treated with 5-azacytidine. Biologia plantarum. 2019; 63 (1):398-404.
Chicago/Turabian StyleK.V. Kiselev; Z.V. Ogneva; A.S. Dubrovina; N.N. Nityagovsky; Andrey Suprun. 2019. "Somatic mutations, DNA methylation, and expression of DNA repair genes in Arabidopsis thaliana treated with 5-azacytidine." Biologia plantarum 63, no. 1: 398-404.
This paper investigated the effects of p-coumaric acid (CA) on stilbene biosynthesis in healthy cuttings of grapevine Vitis amurensis Rupr. under control conditions and after ultraviolet (UV-C) irradiation. It has been shown that the content of the detected six stilbenes (cis-piceid, t-piceid, t-ε-viniferin, cis-ε-viniferin, t-resveratrol, and t-δ-viniferin) increased after feeding with CA up to 0.5 mg g−1 of the dry weight (DW), which is 2.4 times higher than that under control conditions. Feeding with CA and UV-C irradiation exerted a combined positive effect on the content of stilbenes increasing it to 0.7 mg g−1 DW. In addition, the CA treatment improved viability of the cuttings after the UV-C treatment. The elevation in stilbene content induced by CA and UV-C correlated with induction of stilbene biosynthetic genes encoding stilbene synthases, resveratrol O-glucosyltransferase, polyphenol oxidase, and cationic peroxidase. The data indicate that feeding with the precursors of phenolic compounds could be an effective strategy for activation of stilbene production.
Konstantin V. Kiselev; Zlata V. Ogneva; Andrey Suprun; Valeria P. Grigorchuk; Alexandra S. Dubrovina. Action of ultraviolet-C radiation and p-coumaric acid on stilbene accumulation and expression of stilbene biosynthesis-related genes in the grapevine Vitis amurensis Rupr. Acta Physiologiae Plantarum 2019, 41, 28 .
AMA StyleKonstantin V. Kiselev, Zlata V. Ogneva, Andrey Suprun, Valeria P. Grigorchuk, Alexandra S. Dubrovina. Action of ultraviolet-C radiation and p-coumaric acid on stilbene accumulation and expression of stilbene biosynthesis-related genes in the grapevine Vitis amurensis Rupr. Acta Physiologiae Plantarum. 2019; 41 (2):28.
Chicago/Turabian StyleKonstantin V. Kiselev; Zlata V. Ogneva; Andrey Suprun; Valeria P. Grigorchuk; Alexandra S. Dubrovina. 2019. "Action of ultraviolet-C radiation and p-coumaric acid on stilbene accumulation and expression of stilbene biosynthesis-related genes in the grapevine Vitis amurensis Rupr." Acta Physiologiae Plantarum 41, no. 2: 28.
Plant cell and tissue cultures are considered as a source of valuable secondary metabolites but usually produce insufficient level of the compounds, which is the limiting factor for their application in biotechnology. We obtained 18 callus cell cultures from different organs of wild grape Vitis amurensis Rupr. collected at different seasons and analyzed stilbene accumulation in combination with calli growth parameters. This analysis showed that temporal and tissue origin of the calli affected the rate of stilbene biosynthesis. Stem-derived calli accumulated higher stilbene levels and exhibited a higher expression of phenylalanine ammonia-lyase (PAL) and stilbene synthase (STS) genes than calli derived from the leaves and petioles. The highest content of stilbenes was detected in the calli initiated from grapevine stems collected in the autumn. In general, all “autumn” cell cultures contained more than 2 mg g− 1 dry wt (up to 11 mg g− 1 dry wt) and exhibited high PAL and STS genes expression in comparison with the calli initiated in the summer. The content of stilbenes in the “autumn” cell cultures were comparable to the highest stilbene contents detected in other plant sources described in the literature. Thus, selecting the most optimal explant source for cell culture establishment could be an effective approach towards developing plant cell cultures producing high stilbene levels.
A. P. Tyunin; Andrey Suprun; N. N. Nityagovsky; A. Y. Manyakhin; Y. A. Karetin; A. S. Dubrovina; K. V. Kiselev. The effect of explant origin and collection season on stilbene biosynthesis in cell cultures of Vitis amurensis Rupr. Plant Cell, Tissue and Organ Culture (PCTOC) 2018, 136, 189 -196.
AMA StyleA. P. Tyunin, Andrey Suprun, N. N. Nityagovsky, A. Y. Manyakhin, Y. A. Karetin, A. S. Dubrovina, K. V. Kiselev. The effect of explant origin and collection season on stilbene biosynthesis in cell cultures of Vitis amurensis Rupr. Plant Cell, Tissue and Organ Culture (PCTOC). 2018; 136 (1):189-196.
Chicago/Turabian StyleA. P. Tyunin; Andrey Suprun; N. N. Nityagovsky; A. Y. Manyakhin; Y. A. Karetin; A. S. Dubrovina; K. V. Kiselev. 2018. "The effect of explant origin and collection season on stilbene biosynthesis in cell cultures of Vitis amurensis Rupr." Plant Cell, Tissue and Organ Culture (PCTOC) 136, no. 1: 189-196.
It is known that somatic mutations arising during animal growth and ageing contribute to the development of neurodegenerative and other animal diseases. For plants, several studies showed that small-scale somatic DNA mutations accumulated during Arabidopsis life cycle. However, there is a lack of data on the influence of environmental stresses on somatic DNA mutagenesis in plants. In this study, we analyzed the effects of ultraviolet C (UV-C) irradiation, high soil salinity, and cadmium (CdI3) stresses on the level of small-scale somatic DNA mutations in Arabidopsis thaliana. The number of DNA mutations was examined in the Actin2 3′UTR (Actin-U1), ITS1-5.8rRNA-ITS2 (ITS), and ribulose-1,5-biphosphate carboxylase/oxygenase (rbcL) DNA regions. We found that somatic mutation levels considerably increased in CdI3-treated Arabidopsis plants, while the mutation levels declined in the UV-C- and NaCl-treated A. thaliana. Cadmium is a mutagen that is known to inhibit DNA repair processes. The detected stress-induced alterations in somatic DNA mutation levels were accompanied by markedly increased expression of base excision repair genes (AtARP, AtDME, AtDML2, AtDML3, AtMBD4, AtROS, AtUNG, and AtZDP), nucleotide excision repair genes (AtDDB1a, AtRad4, and AtRad23a), mismatch repair genes (AtMSH2, AtMSH3, and AtMSH7), and photoreactivation genes (AtUVR2, AtUVR3). Thus, the results demonstrated that UV-C, high soil salinity, and cadmium stresses influence both the level of DNA mutations and expression of DNA repair genes. Salt- and UV-induced activation of DNA repair genes could contribute to the stress-induced decrease in somatic mutation level.
K. V. Kiselev; Z. V. Ogneva; A. S. Dubrovina; Andrey Suprun; A. P. Tyunin. Altered somatic mutation level and DNA repair gene expression in Arabidopsis thaliana exposed to ultraviolet C, salt, and cadmium stresses. Acta Physiologiae Plantarum 2017, 40, 21 .
AMA StyleK. V. Kiselev, Z. V. Ogneva, A. S. Dubrovina, Andrey Suprun, A. P. Tyunin. Altered somatic mutation level and DNA repair gene expression in Arabidopsis thaliana exposed to ultraviolet C, salt, and cadmium stresses. Acta Physiologiae Plantarum. 2017; 40 (1):21.
Chicago/Turabian StyleK. V. Kiselev; Z. V. Ogneva; A. S. Dubrovina; Andrey Suprun; A. P. Tyunin. 2017. "Altered somatic mutation level and DNA repair gene expression in Arabidopsis thaliana exposed to ultraviolet C, salt, and cadmium stresses." Acta Physiologiae Plantarum 40, no. 1: 21.
Stilbenes are valuable plant phytoalexins, the biosynthesis of which is characteristic of different groups of phylogenetically unrelated plants. It is believed that all the stilbenes are the derivatives of resveratrol (3,5,4'-trihydroxy-trans-stilbene) or compounds close to it (pinosylvin or piceatannol). The last stage of the resveratrol biosynthesis takes place with the involvement of stilbene synthase or resveratrol synthase (STS). The family Pinaceae is characterized by the presence of the derivatives of pinosylvin (genus Pinus) and piceatannol (genus Picea), the biosynthetic pathways of which are scarcely examined. Previously, in different species of the genus Picea, only two stilbene synthase genes were described. On the basis of RNA isolated from the needles of spruce Picea jezoensis, the full-length cDNAs of the four stilbene synthase genes, PjSTS1a, PjSTS1b, PjSTS2, and PjSTS3, were obtained. Then, using the clone frequency analysis and real-time PCR, expression of the PjSTS1a, PjSTS1b, PjSTS2, and PjSTS3 genes was examined in the needles of P. jezoensis accessions of different age and sampled in different seasons (spring, summer, autumn, winter). Among the analyzed transcripts, the PjSTS1a and PjSTS1b genes were the most frequent, indicating their higher level of expression compared to other STS genes. The highest level of PjSTS1a and PjSTS1b expression was observed in autumn, while the level of PjSTS2 and PjSTS3 expression was the highest in spring and winter. Moreover, the highest PjSTS expression was detected in the young tissues of P. jezoensis in autumn, which may indicate a higher level of stilbene biosynthesis in these tissues.
K. V. Kiselev; Z. V. Ogneva; Andrey Suprun; Yu. N. Zhuravlev. [Expression of the stilbene synthase genes in the needles of spruce Picea jezoensis]. Генетика 2016, 52, 1279 -86.
AMA StyleK. V. Kiselev, Z. V. Ogneva, Andrey Suprun, Yu. N. Zhuravlev. [Expression of the stilbene synthase genes in the needles of spruce Picea jezoensis]. Генетика. 2016; 52 (11):1279-86.
Chicago/Turabian StyleK. V. Kiselev; Z. V. Ogneva; Andrey Suprun; Yu. N. Zhuravlev. 2016. "[Expression of the stilbene synthase genes in the needles of spruce Picea jezoensis]." Генетика 52, no. 11: 1279-86.
Stilbenes are valuable phenolic compounds that are synthesized in plants via the phenylpropanoid pathway where stilbene synthase (STS) directly catalyzes resveratrol or pinosylvin formation. Currently, there is a lack of information about the stilbene biosynthetic pathway in spruce (Picea). Resveratrol and piceatannol derivatives have been detected in the spruce bark, needles, and roots. We analyzed seasonal variation in stilbene spectrum and content in the needles of different ages of one tree of spruce Picea jezoensis. HPLC analysis revealed the presence of nine stilbenes: t- and cis-astringin, t- and cis-piceid, t- and cis-isorhapontin, and t-piceatannol were present in amounts of 0.01-6.07 mg/g of dry weight (DW), while t-isorhapontigenin and t-resveratrol were present in traces (0.001-0.312 μg/g DW). T-astringin prevailed over other stilbenoid compounds (66-86% of all stilbenes). The highest total stilbene content was detected in one-year-old needles collected in the autumn and spring (5.4-7.77 mg/g DW). We previously cloned and sequenced full-length cDNAs of the four STS transcripts (PjSTS1a, PjSTS1b, PjSTS2, and PjSTS3) of P. jezoensis. This study presents a detailed analysis of seasonal variations in PjSTS1a, 1b, 2, and 3 transcript levels in the needles of P. jezoensis of different ages using qRT-PCR. PjSTS1a and PjSTS1b transcription was higher in the needles collected in the autumn, spring, or summer than in the winter. PjSTS2 was actively transcribed in the needles of all ages collected in the winter, spring, and summer. PjSTS3 expression did not significantly change during the year and did not depend on the age of the needles. Therefore, the data show that high levels of the stilbene glucosides and PjSTS expression are present in the needles of P. jezoensis.
K.V. Kiselev; V.P. Grigorchuk; Z.V. Ogneva; Andrey Suprun; Alexandra Dubrovina. Stilbene biosynthesis in the needles of spruce Picea jezoensis. Phytochemistry 2016, 131, 57 -67.
AMA StyleK.V. Kiselev, V.P. Grigorchuk, Z.V. Ogneva, Andrey Suprun, Alexandra Dubrovina. Stilbene biosynthesis in the needles of spruce Picea jezoensis. Phytochemistry. 2016; 131 ():57-67.
Chicago/Turabian StyleK.V. Kiselev; V.P. Grigorchuk; Z.V. Ogneva; Andrey Suprun; Alexandra Dubrovina. 2016. "Stilbene biosynthesis in the needles of spruce Picea jezoensis." Phytochemistry 131, no. : 57-67.