(1) Background: Weed control decreases the competition for nutrients, but also the potential of increased phosphorus (P) mobilization in soils caused by higher plant diversity. (2) Methods: Impacts of weed species under maize on mycorrhizal colonization and plant-availability of P were investigated in two pot experiments. Plant traits and P mobilization were tested in weed-free maize and in mixed growth with six annual weed species. (3) Results: Growth of maize decreased in treatments with weeds, while P concentrations in its shoots increased in mixed growth with C. album, E. crus-galli and P. convolvulus. Mycorrhizal colonization of maize without weeds was low (<20% of root length), but increased in mixed growth with C. album, E. crus-galli and V. arvensis up to 40%. The activities of Pmobilizing hydrolytic enzymes (phosphatases, ß-glucosidase) and plant-availability of P were occasionally higher under mixed growth with weeds. The dimension of weed impacts on P cycling under maize differed significantly between both experiments. (4) Conclusions: Weeds potentially promote P mobilization and mycorrhizal colonization under maize, but this impact is not guaranteed. The weed-based improved P supply of maize should be defined under field conditions to allow a controlled weed tolerance in maize cropping systems.

Legume catch crops can enhance soil fertility and promote the N and P supply of the subsequent main crop, especially with low mineral fertilizer use. However, the specific impact of catch crops on arbuscular mycorrhiza formation of the following main crop is unknown. Therefore, the impact of serradella (Ornithopus sativus) vs. bare fallow was tested on mycorrhiza formation, potential soil enzyme activities and plant-available P under subsequently grown barley (Hordeum vulgare) and different fertilization treatments (P-unfertilized—P0; triple superphosphate—TSP; compost—COM; combined—COM + TSP) in a long-term field experiment in northeastern Germany. Catch cropping significantly increased mycorrhiza formation of barley up to 14% compared to bare fallow. The impact of serradella on mycorrhiza formation exceeded that of the fertilization treatment. Serradella led to increased phosphodiesterase activities and decreased ß-glucosidase activities in soil. Plant availability of P was not significantly affected by serradella. These findings provide initial evidence that even serradella as a non-host crop of mycorrhizal fungi can promote the mycorrhiza formation of the subsequent crop and P mobilization in soil. We conclude that the prolonged vegetation cover of arable soils by the use of catch crops can promote P mobilization and transfer from P pools to the following main crops.