The development of new high-power biofuel cells has been limited in the past by slow or indirect charge transfer. In this study, enzymatic biofuel cell (EBFC) systems were explored with different materials used to evaluate their applicability as redox mediators. Redox mediators of different natures have been selected for this research. Cytochrome c, Chlorophyll a, and supernatant of ultrasonically disrupted algae Chlorella vulgaris cells were examined as potential redox mediators. The effect of heparin on the EBFC was also evaluated under the same analytical conditions. The measurements of open circuit potential (OCP) and the evaluation of the current response in two modes of measurement were performed (i) during potential cycling in cyclic voltammetry measurements or (ii) at the constant potential value in chronoamperometry, and were applied for the evaluation of EBFC. Cytochrome c, Chlorophyll a, and the supernatant of ultrasonically disrupted algae Chlorella vulgaris cells-based redox mediators were efficient in the glucose oxidase (GOx) based EBFC. Electron transfer from GOx to the electrode was facilitated through the redox mediators adsorbed on the electrode. Electrodes modified with Chlorophyll a- and Cytochrome c-based redox mediators were suitable for the development of glucose biosensors. This was demonstrated by increasing the glucose concentration within 0 mM–100 mM in the system, the current density increased, and the system reached equilibrium rather faster regarding the electrochemical reaction. The power density is an important feature in revealing the action of biofuel cells. The highest power values were generated by the systems based on the application of redox-mediated Chlorophyll a and the supernatant of ultrasonically disrupted Chlorella vulgaris cells. The surface power density was about 2.5–4.0 µW/cm². Control of a study was performed with a polished graphite electrode and the maximum surface power density was 0.02471 µW/cm².

Electrical power generation by means of electrochemical systems utilizing wastewaters is a global energy challenge tackling technique for which a creation of novel eco-friendly electrode materials is in high relevance. For this purpose a Rhodophyta algae derived activated biochar anode bound with a flaxseeds mucilage binder (5, 10, 20, 30 wt.%) was formed and characterized by thermogravimetric, Brunauer-Emmett-Teller (BET) analysis as well as conductivity and mechanical resistance determination. Activation technique with KOH prior to carbonization at 800 °C of algae was employed to obtain biocarbon with a large surface area. The highest specific surface area of 1298.49 m²/g was obtained with the binder-free sample and had a tendency to decrease with the increase of the binder content. It was estimated that biochar anodes are thermally stable at the temperature of up to 200 °C regardless of binder concentration. The concentration of the binder on the other hand had a significant influence in anodes mechanical resistance and electrical conductance: anode with 30 wt.% of the binder had the highest compressive strength equal to 104 bar; however, the highest conductivity was estimated in anode with 5 wt.% of the binder equal to 58 S/m. It is concluded that anode with 10 wt.% mucilage binder has the optimal properties necessary in MFC utilization.

Increasing production rates of the biomethane lead to increased generation of waste biogases. These gases should be utilized on-site to avoid pollutant emissions to the atmosphere. This study presents a flexible swirl burner (~100 kW) with an adiabatic chamber capable of burning unstable composition waste biogases. The main combustion parameters and chemiluminescence emission spectrums were examined by burning waste biogases containing from 5 to 30 vol% of CH₄ in CO₂ under air, O₂-enriched atmosphere, or with the addition of hydrogen. The tested burner ensured stable combustion of waste biogases with CH₄ content not less than 20 vol%. The addition of up to 5 vol% of H₂ expanded flammability limits, and stable combustion of the mixtures with CH₄ content of 15 vol% was achieved. The burner flexibility to work under O₂-enriched air conditions showed more promising results, and the flammability limit was expanded up to 5 vol% of CH₄ in CO₂. However, the combustion under O₂-enriched conditions led to increased NOx emissions (up to 1100 ppm). Besides, based on chemiluminescence emission spectrums, a linear correlation between the spectral intensity ratio of OH* and CH* (I_OH*/I_CH*) and CH₄ content in CO₂ was presented, which predicts blow-off limits burning waste biogases under different H₂ or O₂ enrichments.

Significant shortfalls in meeting the climate mitigation targets and volatile energy markets make evident the need for an urgent transition from fossil fuels to sustainable alternatives. However, the integration of zero-carbon fuels like green hydrogen and ammonia is an immense project and will take time and the construction of new infrastructure. It is during this transitional period that lower-carbon natural gas alternatives are essential. In this study, the industrial sectors of Lithuania are analysed based on their energy consumption. The industrial sectors that are the most energy-intensive are food, chemical, and wood-product manufacturing. Synthetic natural gas (SNG) has become a viable substitute, and biomethane has also become viable given a feedstock price of 21 EUR/MWh in the twelfth year of operation and 24 EUR/MWh in the eighth year, assuming an electricity price of 140 EUR/MWh and a natural gas price of 50 EUR/MWh. Nevertheless, the scale of investment in hydrogen production is comparable to the scale of investment in the production of other chemical elements; however, hydrogen production is constrained by its high electricity demand—about 3.8 to 4.4 kWh/Nm³—which makes it economically viable only at negative electricity prices. This analysis shows the techno-economic viability of biomethane and the SNG as transition pathways towards a low-carbon energy future.