In 1998, when the PCR technique was already popular, a Japanese company called Eiken Chemical Co., Ltd. designed a method known as the loop-mediated isothermal amplification of DNA (LAMP). The method can produce up to 10⁹ copies of the amplified DNA within less than an hour. It is also highly specific due to the use of two to three pairs of primers (internal, external, and loop), which recognise up to eight specific locations on the DNA or RNA targets. Furthermore, the Bst DNA polymerase most used in LAMP shows a high strand displacement activity, which eliminates the DNA denaturation stage. One of the most significant advantages of LAMP is that it can be conducted at a stable temperature, for instance, in a dry block heater or an incubator. The products of LAMP can be detected much faster than in standard techniques, sometimes only requiring analysis with the naked eye. The following overview highlights the usefulness of LAMP and its effectiveness in various fields; it also considers the superiority of LAMP over PCR and presents RT-LAMP as a rapid diagnostic tool for SARS-CoV-2.

Bivalves play an important part in freshwater ecosystems and improve water quality; the thick-shelled river musselUnio crassusis classified as a bioindicator. Unfortunately, the species is regarded as endangered and is under species protection in the whole of Europe. The reasons for the drastic decline of its populations are: considerable eutrophication of waters, anthropogenic influence in its broad sense and the presence of invasive species. The life cycle ofU. crassusincludes the stage of larva which is an obligatory parasite of fish. This makes it possible for the species to disperse and populate new territories but it limits the development to places where appropriate host species are available. Intensive measures have been taken in Poland to protectU. crassus, while in France numerous new localities of the species have been bar-coded. In 2010–2014 active protection measures were taken in southern Poland, including inventorying, studies of genetic diversity and reintroductions. The project contributed to the increase in population abundance and in the number of localities of the thick-shelled river mussel, which resulted in a twofold increase in the range of occurrence of the species in the river. The procedures presented here can and should be used in further restitution ofU. crassusnot only in Poland but also elsewhere in Europe.

In Poland, the population of Western Pomerania stands out due to the near-total displacement of its inhabitants following World War II. The German population was displaced (1.6 million people), and these lands were subsequently resettled by Poles arriving from various regions of Poland and Europe. Currently, the population of the West Pomeranian Voivodeship is 1.63 million, with 298 unrelated individuals studied. The research aimed to assess the variability of the entire mitochondrial control region (16024..16569, 1..576 bp in rCRS) among the inhabitants of the West Pomeranian Voivodeship and to compare the data with those from European and Polish populations. The study revealed a high level of genetic diversity in mtDNA among the inhabitants of the West Pomeranian Voivodeship, making it one of the most diverse populations in Poland and Europe. A total of 243 haplotypes were identified, with the most common haplogroups being HV, U, T, J, and R0. Within this population, the probability of random matching was 1:145. The molecular parameters obtained indicate a high degree of heterogeneity in the population of the West Pomeranian Voivodeship compared to the neighbouring German population from Fore Pomerania.