The biggest threat to beekeeping is varroosis caused by the mite Varroa destructor. Chemicals available to treat this fatal disease may present problems of resistance or inconsistent efficacy. Recently, lithium chloride has appeared as a potential alternative. To date, the amount of residue lithium treatments may leave in honeybee products is poorly understood. Honeybees were fed with 25 mM lithiated sugar syrup, which was used in earlier studies. The accumulation and elimination of the lithium were monitored in bees and their products for 22 days. Lithium concentration increased in the entire body of the bees to day 4 post-treatment and then recovered rapidly to the control level. Lithium exposure was found to affect uncapped honey in the short term (<16 days), but ripe (capped) honey measured at the end of the trial remained affected. On the other hand, lithium treatment left beeswax lithium-free. Based on these data, we propose that comprehensive research on harvested honey is needed to decide on the veterinary use of lithium.

Electrospinning has recently been recognized as a potential method for use in biomedical applications such as nanofiber-based drug delivery or tissue engineering scaffolds. The present study aimed to demonstrate the electrospinning preparation and suitability of β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration under in vitro and in vivo conditions. The mesh physicochemical properties included a 147 ± 50 nm fibrous structure, in aqueous media the contact angles were 64.1 ± 1.7°, and it released Ca, P, and Si. The viability of dental pulp stem cells on the BTCP-AE-FM was proven by an alamarBlue assay and with a scanning electron microscope. Critical-size calvarial defects in rats were performed as in vivo experiments to investigate the influence of meshes on bone regeneration. PET imaging using ¹⁸F-sodium fluoride standardized uptake values (SUVs) detected 7.40 ± 1.03 using polyvinyl alcohol/chitosan fibrous meshes (FMs) while 10.72 ± 1.11 with BTCP-AE-FMs after 6 months. New bone formations were confirmed by histological analysis. Despite a slight change in the morphology of the mesh because of cross-linking, the BTCP-AE-FM basically retained its fibrous, porous structure and hydrophilic and biocompatible character. Our experiments proved that hybrid nanospun scaffold composite mesh could be a new experimental bone substitute bioactive material in future medical practice.

In this research, contamination levels and the spatial pattern identification, as well as human and environmental health risk assessments of the heavy metals in the phreatic groundwater aquifer of the Oued Souf Valley were investigated for the first time. The applied methodology comprised a combination of heavy-metal pollution indices, inverse distance weighting, and human health risk assessment through water ingestion on samples collected from (14) monitoring wells. The contamination trend in the phreatic aquifer showed Al > B > Sr > Mn > Fe > Pb > Ni > Cr > Ba > Cu > Zn. Similarly, the enrichment trend was Al > B > Sr > Mn > Ni > Pb > Cr > Ba > Cu > Zn. Ecologically, most of the analyzed metals reflected a low potential ecological risk, except for two wells, S13 and S14, which represented a considerable and high ecological risk in terms of Pb. According to the applied grouping method, the samples in the first group indicated a lower risk of contamination in terms of heavy metals due to their lower concentration compared to the second group. This makes the area containing the second group’s samples more vulnerable in terms of heavy metals, which could affect urban, preurban, and even agricultural areas. All of the samples (100%) indicated the possibility of potential health risks in the case of children. While six samples showed that the non-cancer toxicity risk is considered low, the rest of the samples had high Hazard Index (HI) values, indicating the possibility of health risks occurring in the case of adults. The constructed vertical drainage system is acting as a supporter and accelerator of the pollution levels in the shallow groundwater aquifer. This is due to its contribution to the penetration of different pollutants into this aquifer system, depending on the residence time of the water, which appears to be long within the drainage system.

Disposal sites without adequate engineering controls pose a significant risk to the environment. In the present study, the environmental hazards of an abandoned and unrecultivated liquid waste disposal are investigated with a special focus on soil and shallow groundwater contamination. After a period of operation from 1994 to 2010, when the wastewater collection of the municipality was regulated, the disposal site was subsequently decommissioned without further action. Eight monitoring wells have been established in the disposal basins and in the surrounding area to determine the contamination of the site. Sampling took place in the summers of 2020 and 2021. The results of the analysis of the soil and water samples collected showed a high level of contamination in the area. In the borehole profile of the infiltration basin, a well-developed leachate nitrate profile was observed, with a concentration above 3000 mg/kg NO₃⁻. The soil phosphate content was also significant, with a value of over 1900 mg/kg in the upper 40 cm layer. Extremely high concentrations of ammonium (>45 mg/L) and organic matter (>90 mg/L) were detected in the groundwater of the basins, indicating that contaminated soil remains a major source of pollutants more than 10 years after closure. For all micro- and macroelements present in detectable concentrations, a significant increase was observed in the infiltration basin. Our results have revealed that the surroundings are also heavily contaminated. NO₃⁻ concentrations above the contamination limit were measured outside the basins. Recultivation of liquid waste disposal sites of similar characteristics is therefore strongly recommended.

Septic tanks without proper construction and insulation entail a significant risk to the environment. In this study, the environmental impacts of a permeably designed septic tank on shallow groundwater contamination are investigated, and changes in water quality in the period after its elimination in 2014 are assessed. For the purpose of evaluating the pollution level of the site, 10 monitoring wells were installed around the septic tank in 2012 and long-term monitoring was carried out. Analytical measurements revealed a significant level of groundwater contamination in the operational period of the septic tank. Extremely high concentrations of NH₄⁺ (>90 mg/L) were observed in the closest monitoring wells, and in most of the wells, concentrations exceeded the relevant contamination limit. δD and δ¹⁸O isotopic ratios of monitoring wells within 1 m from the septic tank indicate continuous recharge of sewage water originating from deeper aquifers. The groundwater dome resulting from the wastewater discharge exceeded 1.1 m, within a distance of 25 m. Statistical analyses also revealed significant changes in water quality depending on the monitoring well location from the septic tank. In the period after the septic tank elimination, considerable changes have been detected. Following the cessation of the wastewater discharge, the groundwater dome around the septic tank disappeared; therefore, differences in groundwater levels have decreased from more than 1 m to a few cm. Significant positive changes were detected in the water quality parameters investigated after the dismantling of the septic tank. Five years after the cessation of the pollutant supply, concentrations still exceeded the contamination limit in most of the monitoring wells, indicating slow decontamination processes with a permanently high level of pollution.

The applicability of capillary electrophoresis (CE) with light-emitting diode-induced fluorescence detection (LEDIF) for the separation of sugars in honey samples was studied. An amount of 25 mM ammonium acetate (pH 4.5) with 0.3% polyethylene oxide (PEO) was found to be optimal for the efficient separation of carbohydrates. 8-aminopyrene-1,3,6-trisulfonic acid (APTS) was used for the labeling of the carbohydrate standards and honey sugars for fluorescence detection. The optimized method was applied in the quantitative analysis of fructose and glucose by direct injection of honey samples. Apart from the labeling reaction, no other sample preparation was performed. The mean values of the fructose/glucose ratio for phacelia honey, acacia honey and honeydew honey were 0.86, 1.61 and 1.42, respectively. The proposed method provides high separation efficiency and sensitive detection within a short analysis time. Apart from the labeling reaction, it enables the injection of honeys without sample pretreatment. This is the first time that fluorescence detection has been applied for the CE analysis of sugars in honeys.