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Diagnostic methods based on SARS-CoV-2 antigens detection are a promising alternative to SARS-CoV-2 RNA amplification. We evaluated the automated chemiluminescence-based Lumipulse® G SARS-CoV-2 Ag assay on saliva samples, using Simplexa™ COVID-19 Direct assay as a reference test. Analytical performance was established on a pool of healthy donors’ saliva samples spiked with the 2019-nCoV/Italy-INMI1 isolate, whereas clinical performance was assessed on fresh saliva specimens collected from hospitalized patients with suspect or confirmed COVID-19 diagnosis. The limit of detection (LOD) was 0.65 Log TCID50/mL, corresponding to 18,197 copies/mL of SARS-CoV-2 RNA. Antigen concentrations and SARS-CoV-2 RNA were highly correlated (r = 0.99; p < 0.0001). Substantial agreement (80.3%) and significant correlation (r = −0.675; p = 0.0006) were observed between Lumipulse® G assay results and Ct values on clinical samples, with 52.4% sensitivity and specificity 94.1%. Sensitivity exceeded 90.0% when calculated on samples with Ct < 25, and specificity was 100% when excluding samples from recovered patients with previous COVID-19 diagnosis. Overall, chemiluminescence-based antigen assay may be reliably applied to saliva samples to identify individuals with high viral loads, more likely to transmit the virus. However, the low positive predictive value in a context of low SARS-CoV-2 prevalence underscores the need for confirmatory testing in SARS-CoV-2 antigen-positive cases.
Alessandra Amendola; Giuseppe Sberna; Eleonora Lalle; Francesca Colavita; Concetta Castilletti; Giulia Menchinelli; Brunella Posteraro; Maurizio Sanguinetti; Giuseppe Ippolito; Licia Bordi; Maria Capobianchi; on behalf of INMI COVID-19 Study Group. Saliva Is a Valid Alternative to Nasopharyngeal Swab in Chemiluminescence-Based Assay for Detection of SARS-CoV-2 Antigen. Journal of Clinical Medicine 2021, 10, 1471 .
AMA StyleAlessandra Amendola, Giuseppe Sberna, Eleonora Lalle, Francesca Colavita, Concetta Castilletti, Giulia Menchinelli, Brunella Posteraro, Maurizio Sanguinetti, Giuseppe Ippolito, Licia Bordi, Maria Capobianchi, on behalf of INMI COVID-19 Study Group. Saliva Is a Valid Alternative to Nasopharyngeal Swab in Chemiluminescence-Based Assay for Detection of SARS-CoV-2 Antigen. Journal of Clinical Medicine. 2021; 10 (7):1471.
Chicago/Turabian StyleAlessandra Amendola; Giuseppe Sberna; Eleonora Lalle; Francesca Colavita; Concetta Castilletti; Giulia Menchinelli; Brunella Posteraro; Maurizio Sanguinetti; Giuseppe Ippolito; Licia Bordi; Maria Capobianchi; on behalf of INMI COVID-19 Study Group. 2021. "Saliva Is a Valid Alternative to Nasopharyngeal Swab in Chemiluminescence-Based Assay for Detection of SARS-CoV-2 Antigen." Journal of Clinical Medicine 10, no. 7: 1471.
COVID-19 pandemic is a dramatic health, social and economic global challenge. There is urgent need to maximize testing capacity. Rapid Antigen Tests (RAT) represent good candidates for point-of-care and mass surveillance testing to rapidly identify SARS-CoV-2-infected people, counterbalancing lower sensitivity vs. gold standard molecular tests with fast results and possible recurrent testing. We describe the results obtained with the testing algorithm implemented at points of entry (airports and ports) in the Lazio Region (Italy), using the STANDARD F COVID-19 Antigen Fluorescence ImmunoAssay (FIA), followed by molecular confirmation of FIA-positive samples. From mid-August to mid-October 2020, 73,643 RAT were reported to the Regional Surveillance Information System for travelers at points of entry in Lazio Region. Of these, 1176 (1.6%) were FIA-positive, and the proportion of RT-PCR-confirmed samples was 40.5%. Our data show that the probability of confirmation was directly dependent from the semi-quantitative FIA results. In addition, the molecularly confirmed samples were those with high levels of virus and that were actually harboring infectious virus. These results support public health strategies based on early mass screening campaigns by RAT in settings where molecular testing is not feasible or easily accessible, such as points of entry. This approach would contribute to promptly controlling viral spread through travel, which is now of particular concern due to the spread of SARS-CoV-2 variants.
Francesca Colavita; Francesco Vairo; Silvia Meschi; Maria Beatrice Valli; Eleonora Lalle; Concetta Castilletti; Danilo Fusco; Giuseppe Spiga; Pierluigi Bartoletti; Simona Ursino; Maurizio Sanguinetti; Antonino Di Caro; Francesco Vaia; Giuseppe Ippolito; Maria Rosaria Capobianchi. COVID-19 Rapid Antigen Test as Screening Strategy at Points of Entry: Experience in Lazio Region, Central Italy, August–October 2020. Biomolecules 2021, 11, 425 .
AMA StyleFrancesca Colavita, Francesco Vairo, Silvia Meschi, Maria Beatrice Valli, Eleonora Lalle, Concetta Castilletti, Danilo Fusco, Giuseppe Spiga, Pierluigi Bartoletti, Simona Ursino, Maurizio Sanguinetti, Antonino Di Caro, Francesco Vaia, Giuseppe Ippolito, Maria Rosaria Capobianchi. COVID-19 Rapid Antigen Test as Screening Strategy at Points of Entry: Experience in Lazio Region, Central Italy, August–October 2020. Biomolecules. 2021; 11 (3):425.
Chicago/Turabian StyleFrancesca Colavita; Francesco Vairo; Silvia Meschi; Maria Beatrice Valli; Eleonora Lalle; Concetta Castilletti; Danilo Fusco; Giuseppe Spiga; Pierluigi Bartoletti; Simona Ursino; Maurizio Sanguinetti; Antonino Di Caro; Francesco Vaia; Giuseppe Ippolito; Maria Rosaria Capobianchi. 2021. "COVID-19 Rapid Antigen Test as Screening Strategy at Points of Entry: Experience in Lazio Region, Central Italy, August–October 2020." Biomolecules 11, no. 3: 425.
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Franca del Nonno; Andrea Frustaci; Romina Verardo; Cristina Chimenti; Emanuele Nicastri; Andrea Antinori; Nicola Petrosillo; Eleonora Lalle; Chiara Agrati; Giuseppe Ippolito; and on behalf of the INMI COVID study group. Virus-Negative Myopericarditis in Human Coronavirus Infection. Circulation: Heart Failure 2020, 13, e007636 -e007636.
AMA StyleFranca del Nonno, Andrea Frustaci, Romina Verardo, Cristina Chimenti, Emanuele Nicastri, Andrea Antinori, Nicola Petrosillo, Eleonora Lalle, Chiara Agrati, Giuseppe Ippolito, and on behalf of the INMI COVID study group. Virus-Negative Myopericarditis in Human Coronavirus Infection. Circulation: Heart Failure. 2020; 13 (11):e007636-e007636.
Chicago/Turabian StyleFranca del Nonno; Andrea Frustaci; Romina Verardo; Cristina Chimenti; Emanuele Nicastri; Andrea Antinori; Nicola Petrosillo; Eleonora Lalle; Chiara Agrati; Giuseppe Ippolito; and on behalf of the INMI COVID study group. 2020. "Virus-Negative Myopericarditis in Human Coronavirus Infection." Circulation: Heart Failure 13, no. 11: e007636-e007636.
Background: RT-PCR on nasopharyngeal (NPS)/oropharyngeal swabs is the gold standard for diagnosis of SARS-CoV-2 infection and viral load monitoring. Oral fluid (OF) is an alternate clinical sample, easy and safer to collect and could be useful for COVID-19 diagnosis, monitoring viral load and shedding. Methods: Optimal assay conditions and analytical sensitivity were established for the commercial Simplexa™ COVID-19 Direct assay adapted to OF matrix. The assay was used to test 337 OF and NPS specimens collected in parallel from 164 hospitalized patients; 50 bronchoalveolar lavage (BAL) specimens from a subgroup of severe COVID-19 cases were also analysed. Results: Using Simplexa™ COVID-19 Direct on OF matrix, 100% analytical detection down to 1 TCID50/mL (corresponding to 4 × 103 copies (cp)/mL) was observed. No crossreaction with other viruses transmitted through the respiratory toute was observed. Parallel testing of 337 OF and NPS samples showed highly concordant results (κ = 0.831; 95 % CI = 0.771–0.891), and high correlation of Ct values (r = 0.921; p < 0.0001). High concordance and elevated correlation was observed also between OF and BAL. Prolonged viral RNA shedding was observed up to 100 days from symptoms onset (DSO), with 32% and 29% positivity observed in OF and NPS samples, respectively, collected between 60 and 100 DSO. Conclusions: Simplexa™ COVID-19 Direct assays on OF have high sensitivity and specificity to detect SARS-CoV-2 RNA and provide an alternative to NPS for diagnosis and monitoring SARS-CoV-2 shedding.
Licia Bordi; Giuseppe Sberna; Eleonora Lalle; Pierluca Piselli; Francesca Colavita; Emanuele Nicastri; Andrea Antinori; Evangelo Boumis; Nicola Petrosillo; Luisa Marchioni; Giulia Minnucci; Elena D’Agostini; Concetta Castilletti; Franco Locatelli; Alimuddin Zumla; Giuseppe Ippolito; Maria Capobianchi; on behalf of INMI ReCOVeRI Study Group. Frequency and Duration of SARS-CoV-2 Shedding in Oral Fluid Samples Assessed by a Modified Commercial Rapid Molecular Assay. Viruses 2020, 12, 1184 .
AMA StyleLicia Bordi, Giuseppe Sberna, Eleonora Lalle, Pierluca Piselli, Francesca Colavita, Emanuele Nicastri, Andrea Antinori, Evangelo Boumis, Nicola Petrosillo, Luisa Marchioni, Giulia Minnucci, Elena D’Agostini, Concetta Castilletti, Franco Locatelli, Alimuddin Zumla, Giuseppe Ippolito, Maria Capobianchi, on behalf of INMI ReCOVeRI Study Group. Frequency and Duration of SARS-CoV-2 Shedding in Oral Fluid Samples Assessed by a Modified Commercial Rapid Molecular Assay. Viruses. 2020; 12 (10):1184.
Chicago/Turabian StyleLicia Bordi; Giuseppe Sberna; Eleonora Lalle; Pierluca Piselli; Francesca Colavita; Emanuele Nicastri; Andrea Antinori; Evangelo Boumis; Nicola Petrosillo; Luisa Marchioni; Giulia Minnucci; Elena D’Agostini; Concetta Castilletti; Franco Locatelli; Alimuddin Zumla; Giuseppe Ippolito; Maria Capobianchi; on behalf of INMI ReCOVeRI Study Group. 2020. "Frequency and Duration of SARS-CoV-2 Shedding in Oral Fluid Samples Assessed by a Modified Commercial Rapid Molecular Assay." Viruses 12, no. 10: 1184.
Filoviruses have become a worldwide public health concern, especially during the 2013-2016 Western Africa Ebola virus disease (EVD) outbreak-the largest outbreak, both by number of cases and geographical extension, recorded so far in medical history. EVD is associated with pathologies in several organs, including the liver, kidney, and lung. During the 2013-2016 Western Africa outbreak, Ebola virus (EBOV) was detected in the lung of infected patients suggesting a role in lung pathogenesis. However, little is known about lung pathogenesis and the controversial issue of aerosol transmission in EVD. This review highlights the pulmonary involvement in EVD, with a special focus on the new data emerging from the 2013-2016 Ebola outbreak.
Eleonora Lalle; Mirella Biava; Emanuele Nicastri; Francesca Colavita; Antonino Di Caro; Francesco Vairo; Simone Lanini; Concetta Castilletti; Martin Langer; Alimuddin Zumla; Gary Kobinger; Maria R. Capobianchi; Giuseppe Ippolito. Pulmonary Involvement during the Ebola Virus Disease. Viruses 2019, 11, 780 .
AMA StyleEleonora Lalle, Mirella Biava, Emanuele Nicastri, Francesca Colavita, Antonino Di Caro, Francesco Vairo, Simone Lanini, Concetta Castilletti, Martin Langer, Alimuddin Zumla, Gary Kobinger, Maria R. Capobianchi, Giuseppe Ippolito. Pulmonary Involvement during the Ebola Virus Disease. Viruses. 2019; 11 (9):780.
Chicago/Turabian StyleEleonora Lalle; Mirella Biava; Emanuele Nicastri; Francesca Colavita; Antonino Di Caro; Francesco Vairo; Simone Lanini; Concetta Castilletti; Martin Langer; Alimuddin Zumla; Gary Kobinger; Maria R. Capobianchi; Giuseppe Ippolito. 2019. "Pulmonary Involvement during the Ebola Virus Disease." Viruses 11, no. 9: 780.
Ebola virus (EBOV) infection is characterized by an excessive inflammatory response, a loss of lymphocytes and a general paralysis of the immune system, however pathophysiological mechanisms are not fully understood. In a cohort of 23 fatal and 21 survivors of ebola virus disease (EVD) cases admitted to the Emergency Ebola-Treatment-Center in Goderich (Freetown, Sierra Leone) during the 2014 to 2016 EBOV epidemic in Western Africa, we analyzed the pathway-focused gene expression profile of secreted proteins involved in the immune response and the levels of specific anti-EBOV IgM and IgG from the time of admission till discharge or death. We observed a dysregulated inflammatory response in fatal patients as compared to survivors, mainly consisting of the upregulation of inflammatory mediators, whose extent directly correlated with viremia levels. The upregulation persisted and intensified during the late phase of infection. Relevant differences were also found in humoral immunity, as an earlier and more robust EBOV antibody response was observed in survivor patients.
Francesca Colavita; Mirella Biava; Concetta Castilletti; Simone Lanini; Rossella Miccio; Gina Portella; Francesco Vairo; Giuseppe Ippolito; Maria Rosaria Capobianchi; Antonino Di Caro; Eleonora Lalle. Inflammatory and Humoral Immune Response during Ebola Virus Infection in Survivor and Fatal Cases Occurred in Sierra Leone during the 2014–2016 Outbreak in West Africa. Viruses 2019, 11, 373 .
AMA StyleFrancesca Colavita, Mirella Biava, Concetta Castilletti, Simone Lanini, Rossella Miccio, Gina Portella, Francesco Vairo, Giuseppe Ippolito, Maria Rosaria Capobianchi, Antonino Di Caro, Eleonora Lalle. Inflammatory and Humoral Immune Response during Ebola Virus Infection in Survivor and Fatal Cases Occurred in Sierra Leone during the 2014–2016 Outbreak in West Africa. Viruses. 2019; 11 (4):373.
Chicago/Turabian StyleFrancesca Colavita; Mirella Biava; Concetta Castilletti; Simone Lanini; Rossella Miccio; Gina Portella; Francesco Vairo; Giuseppe Ippolito; Maria Rosaria Capobianchi; Antonino Di Caro; Eleonora Lalle. 2019. "Inflammatory and Humoral Immune Response during Ebola Virus Infection in Survivor and Fatal Cases Occurred in Sierra Leone during the 2014–2016 Outbreak in West Africa." Viruses 11, no. 4: 373.
Chikungunya virus (CHIKV) is a re-emerging mosquito-borne virus that displays a large cell and organ tropism, and causes a broad range of clinical symptoms in humans. It is maintained in nature through both urban and sylvatic cycles, involving mosquito vectors and human or vertebrate animal hosts. Although CHIKV was first isolated in 1953, its pathogenesis was only more extensively studied after its re-emergence in 2004. The unexpected spread of CHIKV to novel tropical and non-tropical areas, in some instances driven by newly competent vectors, evidenced the vulnerability of new territories to this infectious agent and its associated diseases. The comprehension of the exact CHIKV target cells and organs, mechanisms of pathogenesis, and spectrum of both competitive vectors and animal hosts is pivotal for the design of effective therapeutic strategies, vector control measures, and eradication actions.
Giulia Matusali; Francesca Colavita; Licia Bordi; Eleonora Lalle; Giuseppe Ippolito; Maria R. Capobianchi; Concetta Castilletti. Tropism of the Chikungunya Virus. Viruses 2019, 11, 175 .
AMA StyleGiulia Matusali, Francesca Colavita, Licia Bordi, Eleonora Lalle, Giuseppe Ippolito, Maria R. Capobianchi, Concetta Castilletti. Tropism of the Chikungunya Virus. Viruses. 2019; 11 (2):175.
Chicago/Turabian StyleGiulia Matusali; Francesca Colavita; Licia Bordi; Eleonora Lalle; Giuseppe Ippolito; Maria R. Capobianchi; Concetta Castilletti. 2019. "Tropism of the Chikungunya Virus." Viruses 11, no. 2: 175.
Blood donation screening for West Nile virus (WNV) was mandatory in the Lazio region in 2017 and 2018 (June-November) according to the national surveillance plan. In these years, all five donations reactive in WNV nucleic acid amplification tests harboured instead Usutu virus (USUV). Clade ‘Europe 2’ was identified in four blood donations and a 2018 mosquito pool. The cocirculation of WNV and USUV in Lazio warrants increased laboratory support and awareness of possible virus misidentification.
Fabrizio Carletti; Francesca Colavita; Francesca Rovida; Elena Percivalle; Fausto Baldanti; Ida Ricci; Claudio De Liberato; Francesca Rosone; Francesco Messina; Eleonora Lalle; Licia Bordi; Francesco Vairo; Maria Rosaria Capobianchi; Giuseppe Ippolito; Giuseppina Cappiello; Alberto Spanò; Silvia Meschi; Concetta Castilletti. Expanding Usutu virus circulation in Italy: detection in the Lazio region, central Italy, 2017 to 2018. Eurosurveillance 2019, 24, 1800649 .
AMA StyleFabrizio Carletti, Francesca Colavita, Francesca Rovida, Elena Percivalle, Fausto Baldanti, Ida Ricci, Claudio De Liberato, Francesca Rosone, Francesco Messina, Eleonora Lalle, Licia Bordi, Francesco Vairo, Maria Rosaria Capobianchi, Giuseppe Ippolito, Giuseppina Cappiello, Alberto Spanò, Silvia Meschi, Concetta Castilletti. Expanding Usutu virus circulation in Italy: detection in the Lazio region, central Italy, 2017 to 2018. Eurosurveillance. 2019; 24 (3):1800649.
Chicago/Turabian StyleFabrizio Carletti; Francesca Colavita; Francesca Rovida; Elena Percivalle; Fausto Baldanti; Ida Ricci; Claudio De Liberato; Francesca Rosone; Francesco Messina; Eleonora Lalle; Licia Bordi; Francesco Vairo; Maria Rosaria Capobianchi; Giuseppe Ippolito; Giuseppina Cappiello; Alberto Spanò; Silvia Meschi; Concetta Castilletti. 2019. "Expanding Usutu virus circulation in Italy: detection in the Lazio region, central Italy, 2017 to 2018." Eurosurveillance 24, no. 3: 1800649.
Chikungunya fever is caused by Chikungunya virus (CHIKV) and is generally considered a self-limiting disease. However, severe clinical presentations with a high mortality rate have been reported in association with underlying medical conditions. This study reports the molecular characterization of the virus and an abnormal pattern of circulating cytokines in a unique lethal CHIKV case during the 2017 outbreak in Italy, which involved an elderly patient with underlying cardiac disease. Analysis of inflammatory cytokines revealed a strong increase of interferon (IFN)-α and IFN-β, as well as interleukin-6, suggesting a possible role of type-I IFN in the cytokine storm, which may be correlated with unfavorable prognosis of CHIKV infection.
Francesca Colavita; Serena Vita; Eleonora Lalle; Fabrizio Carletti; Licia Bordi; Donatella Vincenti; Irene Pozzetto; Massimo Aiuti; Francesco Vairo; Maria Rosaria Capobianchi; Miriam Lichtner; Concetta Castilletti. Overproduction of IL-6 and Type-I IFN in a Lethal Case of Chikungunya Virus Infection in an Elderly Man During the 2017 Italian Outbreak. Open Forum Infectious Diseases 2018, 5, 1 .
AMA StyleFrancesca Colavita, Serena Vita, Eleonora Lalle, Fabrizio Carletti, Licia Bordi, Donatella Vincenti, Irene Pozzetto, Massimo Aiuti, Francesco Vairo, Maria Rosaria Capobianchi, Miriam Lichtner, Concetta Castilletti. Overproduction of IL-6 and Type-I IFN in a Lethal Case of Chikungunya Virus Infection in an Elderly Man During the 2017 Italian Outbreak. Open Forum Infectious Diseases. 2018; 5 (11):1.
Chicago/Turabian StyleFrancesca Colavita; Serena Vita; Eleonora Lalle; Fabrizio Carletti; Licia Bordi; Donatella Vincenti; Irene Pozzetto; Massimo Aiuti; Francesco Vairo; Maria Rosaria Capobianchi; Miriam Lichtner; Concetta Castilletti. 2018. "Overproduction of IL-6 and Type-I IFN in a Lethal Case of Chikungunya Virus Infection in an Elderly Man During the 2017 Italian Outbreak." Open Forum Infectious Diseases 5, no. 11: 1.
The recent epidemic in the Americas caused by Zika virus (ZIKV), Asian lineage, spurred the research towards a better understanding of how ZIKV infection affects the host immune response. The aim of this study was to evaluate the effects of Asian and East African ZIKV strain infection on the induction of IFN and proinflammatory and Th2 cytokines in human PBMC. We reported a slight modulation of type II IFN in PBMC exposed to Asian strain, but not to African strain, and a complete lack of type I and III IFN induction by both strains, suggesting the ability of ZIKV to evade the IFN system not only inhibiting the antiviral IFN response but also IFN production. Moreover, we highlighted a polyfunctional immune activation only in PBMC exposed to Asian strain, due to the induction of an inflammatory profile (IL-6, IL-8) and of a Th9 (IL-9) response. Overall, our data show a different ability of the ZIKV Asian strain, with respect to the African strain, to activate host immune response that may have pathogenetic implications for virus spread in vivo, including mother-to-child transmission and induction of severe fetal complications, as birth defects and neurological disorders.
Francesca Colavita; Veronica Bordoni; Claudia Caglioti; Mirella Biava; Concetta Castilletti; Licia Bordi; Serena Quartu; Marco Iannetta; Giuseppe Ippolito; Chiara Agrati; Maria Rosaria Capobianchi; Eleonora Lalle. ZIKV Infection Induces an Inflammatory Response but Fails to Activate Types I, II, and III IFN Response in Human PBMC. Mediators of Inflammation 2018, 2018, 1 -6.
AMA StyleFrancesca Colavita, Veronica Bordoni, Claudia Caglioti, Mirella Biava, Concetta Castilletti, Licia Bordi, Serena Quartu, Marco Iannetta, Giuseppe Ippolito, Chiara Agrati, Maria Rosaria Capobianchi, Eleonora Lalle. ZIKV Infection Induces an Inflammatory Response but Fails to Activate Types I, II, and III IFN Response in Human PBMC. Mediators of Inflammation. 2018; 2018 ():1-6.
Chicago/Turabian StyleFrancesca Colavita; Veronica Bordoni; Claudia Caglioti; Mirella Biava; Concetta Castilletti; Licia Bordi; Serena Quartu; Marco Iannetta; Giuseppe Ippolito; Chiara Agrati; Maria Rosaria Capobianchi; Eleonora Lalle. 2018. "ZIKV Infection Induces an Inflammatory Response but Fails to Activate Types I, II, and III IFN Response in Human PBMC." Mediators of Inflammation 2018, no. : 1-6.
This study reports the presence of dengue virus RNA in longitudinally collected semen samples of a previously healthy Caucasian man, returning to Italy from Thailand with primary dengue fever, up to 37 days post-symptom onset, when viraemia and viruria were undetectable. This finding, coupled with the evidence of dengue virus negative-strand RNA, an indirect marker of ongoing viral replication, in the cellular fraction of semen, indicates a need to further investigate possible sexual transmission.
Eleonora Lalle; Francesca Colavita; Marco Iannetta; Saba Gebremeskel Tekle; Fabrizio Carletti; Laura Scorzolini; Licia Bordi; Donatella Vincenti; Concetta Castilletti; Giuseppe Ippolito; Maria Rosaria Capobianchi; Emanuele Nicastri. Prolonged detection of dengue virus RNA in the semen of a man returning from Thailand to Italy, January 2018. Eurosurveillance 2018, 23, 18-00197 .
AMA StyleEleonora Lalle, Francesca Colavita, Marco Iannetta, Saba Gebremeskel Tekle, Fabrizio Carletti, Laura Scorzolini, Licia Bordi, Donatella Vincenti, Concetta Castilletti, Giuseppe Ippolito, Maria Rosaria Capobianchi, Emanuele Nicastri. Prolonged detection of dengue virus RNA in the semen of a man returning from Thailand to Italy, January 2018. Eurosurveillance. 2018; 23 (18):18-00197.
Chicago/Turabian StyleEleonora Lalle; Francesca Colavita; Marco Iannetta; Saba Gebremeskel Tekle; Fabrizio Carletti; Laura Scorzolini; Licia Bordi; Donatella Vincenti; Concetta Castilletti; Giuseppe Ippolito; Maria Rosaria Capobianchi; Emanuele Nicastri. 2018. "Prolonged detection of dengue virus RNA in the semen of a man returning from Thailand to Italy, January 2018." Eurosurveillance 23, no. 18: 18-00197.
We report partial molecular characterization of isolates from an autochthonous chikungunya virus cluster in the Latium Region of Italy. E1 sequences from 3 patients differ substantially from sequences from the 2007 outbreak in Italy and lack the A226V substitution associated with increased viral fitness in the Aedes albopictus mosquito vector.
Licia Bordi; Fabrizio Carletti; Eleonora Lalle; Francesca Colavita; Silvia Meschi; Antonino Di Caro; Emanuele Nicastri; Paola Scognamiglio; Francesco Vairo; Domenico Di Lallo; Vincenzo Panella; Maria R. Capobianchi; Giuseppe Ippolito; Concetta Castilletti. Molecular Characterization of Autochthonous Chikungunya Cluster in Latium Region, Italy. Emerging Infectious Diseases 2018, 24, 178 -180.
AMA StyleLicia Bordi, Fabrizio Carletti, Eleonora Lalle, Francesca Colavita, Silvia Meschi, Antonino Di Caro, Emanuele Nicastri, Paola Scognamiglio, Francesco Vairo, Domenico Di Lallo, Vincenzo Panella, Maria R. Capobianchi, Giuseppe Ippolito, Concetta Castilletti. Molecular Characterization of Autochthonous Chikungunya Cluster in Latium Region, Italy. Emerging Infectious Diseases. 2018; 24 (1):178-180.
Chicago/Turabian StyleLicia Bordi; Fabrizio Carletti; Eleonora Lalle; Francesca Colavita; Silvia Meschi; Antonino Di Caro; Emanuele Nicastri; Paola Scognamiglio; Francesco Vairo; Domenico Di Lallo; Vincenzo Panella; Maria R. Capobianchi; Giuseppe Ippolito; Concetta Castilletti. 2018. "Molecular Characterization of Autochthonous Chikungunya Cluster in Latium Region, Italy." Emerging Infectious Diseases 24, no. 1: 178-180.
Hepatitis C virus (HCV) persistence results from inefficiencies of both innate and adaptive immune responses to eradicate the infection. A functional impairment of circulating Vγ9Vδ2 T-cells was described but few data are available on Vγ9Vδ2 T-cells in the liver that, however, represents the battlefield in the HCV/host interaction. Aim of this work was to compare circulating and intrahepatic Vγ9Vδ2 T-cells in chronic HCV-infected patients (HCV(pos)) and in HCV-negative (HCV(neg)) subjects. Phenotypic and functional analysis was performed by flow cytometry. Anti-HCV activity was analyzed by using an in vitro autologous liver culture system. Independently from HCV infection, the liver was enriched of Vγ9Vδ2 T-cells expressing an effector/activated phenotype. In contrast, an enrichment of PD-1 expressing Vγ9Vδ2 T-cells was observed both in the peripheral blood and in the liver of HCV(pos) patients, probably due to a persistent antigenic stimulation. Moreover, a lower frequency of IFN-γ producing Vγ9Vδ2 T-cells was observed in the liver of HCV(pos) patients, suggesting a functional impairment in the cytokine production in HCV(pos) liver. Despite this hypo-responsiveness, intrahepatic Vγ9Vδ2 T-cells are able to exert an anti-HCV activity after specific stimulation. Altogether, our data show that HCV infection induced a dysregulation of intrahepatic Vγ9Vδ2 T cells that maintain their anti-HCV activity after specific stimulation. A study aimed to evaluate the mechanisms of the antiviral activity may be useful to identify new pathways able to improve Vγ9Vδ2 T-cells intrahepatic function during HCV infection.
E. Cimini; V. Bordoni; A. Sacchi; U. Visco-Comandini; M. Montalbano; C. Taibi; R. Casetti; Eleonora Lalle; G. D’Offizi; M.R. Capobianchi; C. Agrati. Intrahepatic Vγ9Vδ2 T-cells from HCV-infected patients show an exhausted phenotype but can inhibit HCV replication. Virus Research 2018, 243, 31 -35.
AMA StyleE. Cimini, V. Bordoni, A. Sacchi, U. Visco-Comandini, M. Montalbano, C. Taibi, R. Casetti, Eleonora Lalle, G. D’Offizi, M.R. Capobianchi, C. Agrati. Intrahepatic Vγ9Vδ2 T-cells from HCV-infected patients show an exhausted phenotype but can inhibit HCV replication. Virus Research. 2018; 243 ():31-35.
Chicago/Turabian StyleE. Cimini; V. Bordoni; A. Sacchi; U. Visco-Comandini; M. Montalbano; C. Taibi; R. Casetti; Eleonora Lalle; G. D’Offizi; M.R. Capobianchi; C. Agrati. 2018. "Intrahepatic Vγ9Vδ2 T-cells from HCV-infected patients show an exhausted phenotype but can inhibit HCV replication." Virus Research 243, no. : 31-35.
We report here the genome sequence of a human chikungunya virus isolate from the ongoing autochthonous outbreak in central Italy. The sequence (East-Central-South African lineage, Indian Ocean sublineage), which is similar to recent sequences from Pakistan and India, shows E1 and E2 signatures of strains whose main mosquito vector is Aedes aegypti , although Aedes albopictus is the vector in Italy.
Fabrizio Carletti; Patrizia Marsella; Francesca Colavita; Silvia Meschi; Eleonora Lalle; Licia Bordi; Domenico Di Lallo; Vincenzo Panella; Antonino Di Caro; Emanuele Nicastri; Paola Scognamiglio; Simone Lanini; Francesco Vairo; Maria R. Capobianchi; Giuseppe Ippolito; Concetta Castilletti. Full-Length Genome Sequence of a Chikungunya Virus Isolate from the 2017 Autochthonous Outbreak, Lazio Region, Italy. Genome Announcements 2017, 5, e01306-17 .
AMA StyleFabrizio Carletti, Patrizia Marsella, Francesca Colavita, Silvia Meschi, Eleonora Lalle, Licia Bordi, Domenico Di Lallo, Vincenzo Panella, Antonino Di Caro, Emanuele Nicastri, Paola Scognamiglio, Simone Lanini, Francesco Vairo, Maria R. Capobianchi, Giuseppe Ippolito, Concetta Castilletti. Full-Length Genome Sequence of a Chikungunya Virus Isolate from the 2017 Autochthonous Outbreak, Lazio Region, Italy. Genome Announcements. 2017; 5 (49):e01306-17.
Chicago/Turabian StyleFabrizio Carletti; Patrizia Marsella; Francesca Colavita; Silvia Meschi; Eleonora Lalle; Licia Bordi; Domenico Di Lallo; Vincenzo Panella; Antonino Di Caro; Emanuele Nicastri; Paola Scognamiglio; Simone Lanini; Francesco Vairo; Maria R. Capobianchi; Giuseppe Ippolito; Concetta Castilletti. 2017. "Full-Length Genome Sequence of a Chikungunya Virus Isolate from the 2017 Autochthonous Outbreak, Lazio Region, Italy." Genome Announcements 5, no. 49: e01306-17.
Mirella Biava; Claudia Caglioti; Concetta Castilletti; Licia Bordi; Fabrizio Carletti; Francesca Colavita; Serena Quartu; Emanuele Nicastri; Marco Iannetta; Francesco Vairo; Giuseppina Liuzzi; Fabrizio Taglietti; Giuseppe Ippolito; Maria Rosaria Capobianchi; Eleonora Lalle. Persistence of ZIKV-RNA in the cellular fraction of semen is accompanied by a surrogate-marker of viral replication. Diagnostic implications for sexual transmission. The new microbiologica 2017, 40, 1 .
AMA StyleMirella Biava, Claudia Caglioti, Concetta Castilletti, Licia Bordi, Fabrizio Carletti, Francesca Colavita, Serena Quartu, Emanuele Nicastri, Marco Iannetta, Francesco Vairo, Giuseppina Liuzzi, Fabrizio Taglietti, Giuseppe Ippolito, Maria Rosaria Capobianchi, Eleonora Lalle. Persistence of ZIKV-RNA in the cellular fraction of semen is accompanied by a surrogate-marker of viral replication. Diagnostic implications for sexual transmission. The new microbiologica. 2017; 40 (4):1.
Chicago/Turabian StyleMirella Biava; Claudia Caglioti; Concetta Castilletti; Licia Bordi; Fabrizio Carletti; Francesca Colavita; Serena Quartu; Emanuele Nicastri; Marco Iannetta; Francesco Vairo; Giuseppina Liuzzi; Fabrizio Taglietti; Giuseppe Ippolito; Maria Rosaria Capobianchi; Eleonora Lalle. 2017. "Persistence of ZIKV-RNA in the cellular fraction of semen is accompanied by a surrogate-marker of viral replication. Diagnostic implications for sexual transmission." The new microbiologica 40, no. 4: 1.
We describe the dynamics of dengue virus (DENV) infection in a woman in her mid-30s who developed fever after returning from Sri Lanka to Italy in April 2017. Laboratory testing demonstrated detectable DENV-RNA in plasma, urine, saliva, vaginal secretion. Persistent shedding of DENV-RNA was demonstrated in vaginal secretion, and DENV-RNA was detectable in the pelleted fraction up to 18 days from symptom onset. These findings give new insights into DENV vaginal shedding and vertical transmission.
Marco Iannetta; Eleonora Lalle; Maria Musso; Fabrizio Carletti; Laura Scorzolini; Alessandra D'Abramo; Pierangelo Chinello; Concetta Castilletti; Giuseppe Ippolito; Maria R Capobianchi; Emanuele Nicastri. Persistent detection of dengue virus RNA in vaginal secretion of a woman returning from Sri Lanka to Italy, April 2017. Eurosurveillance 2017, 22, 30600 .
AMA StyleMarco Iannetta, Eleonora Lalle, Maria Musso, Fabrizio Carletti, Laura Scorzolini, Alessandra D'Abramo, Pierangelo Chinello, Concetta Castilletti, Giuseppe Ippolito, Maria R Capobianchi, Emanuele Nicastri. Persistent detection of dengue virus RNA in vaginal secretion of a woman returning from Sri Lanka to Italy, April 2017. Eurosurveillance. 2017; 22 (34):30600.
Chicago/Turabian StyleMarco Iannetta; Eleonora Lalle; Maria Musso; Fabrizio Carletti; Laura Scorzolini; Alessandra D'Abramo; Pierangelo Chinello; Concetta Castilletti; Giuseppe Ippolito; Maria R Capobianchi; Emanuele Nicastri. 2017. "Persistent detection of dengue virus RNA in vaginal secretion of a woman returning from Sri Lanka to Italy, April 2017." Eurosurveillance 22, no. 34: 30600.
The recent Ebola outbreak in West Africa caused breakdowns in public health systems, which might have caused outbreaks of vaccine-preventable diseases. We tested 80 patients admitted to an Ebola treatment center in Freetown, Sierra Leone, for measles. These patients were negative for Ebola virus. Measles virus IgM was detected in 13 (16%) of the patients.
Francesca Colavita; Mirella Biava; Concetta Castilletti; Serena Quartu; Francesco Vairo; Claudia Caglioti; Chiara Agrati; Eleonora Lalle; Licia Bordi; Simone Lanini; Michela Delli Guanti; Rossella Miccio; Giuseppe Ippolito; Maria R. Capobianchi; Antonino Di Caro; the Lazzaro Spallanzani Institute for Research and Health Care Ebola Virus Disease Sierra Leone Study Group. Measles Cases during Ebola Outbreak, West Africa, 2013–2106. Emerging Infectious Diseases 2017, 23, 1035 -1037.
AMA StyleFrancesca Colavita, Mirella Biava, Concetta Castilletti, Serena Quartu, Francesco Vairo, Claudia Caglioti, Chiara Agrati, Eleonora Lalle, Licia Bordi, Simone Lanini, Michela Delli Guanti, Rossella Miccio, Giuseppe Ippolito, Maria R. Capobianchi, Antonino Di Caro, the Lazzaro Spallanzani Institute for Research and Health Care Ebola Virus Disease Sierra Leone Study Group. Measles Cases during Ebola Outbreak, West Africa, 2013–2106. Emerging Infectious Diseases. 2017; 23 (6):1035-1037.
Chicago/Turabian StyleFrancesca Colavita; Mirella Biava; Concetta Castilletti; Serena Quartu; Francesco Vairo; Claudia Caglioti; Chiara Agrati; Eleonora Lalle; Licia Bordi; Simone Lanini; Michela Delli Guanti; Rossella Miccio; Giuseppe Ippolito; Maria R. Capobianchi; Antonino Di Caro; the Lazzaro Spallanzani Institute for Research and Health Care Ebola Virus Disease Sierra Leone Study Group. 2017. "Measles Cases during Ebola Outbreak, West Africa, 2013–2106." Emerging Infectious Diseases 23, no. 6: 1035-1037.
Licia Bordi; Tatjana Avsic-Zupanc; Eleonora Lalle; Francesco Vairo; Maria Rosaria Capobianchi; Pedro Fernando Da Costa Vasconcelos. Erratum: Emerging Zika Virus Infection: A Rapidly Evolving Situation. Advances in Experimental Medicine and Biology 2017, 972, 141 -141.
AMA StyleLicia Bordi, Tatjana Avsic-Zupanc, Eleonora Lalle, Francesco Vairo, Maria Rosaria Capobianchi, Pedro Fernando Da Costa Vasconcelos. Erratum: Emerging Zika Virus Infection: A Rapidly Evolving Situation. Advances in Experimental Medicine and Biology. 2017; 972 ():141-141.
Chicago/Turabian StyleLicia Bordi; Tatjana Avsic-Zupanc; Eleonora Lalle; Francesco Vairo; Maria Rosaria Capobianchi; Pedro Fernando Da Costa Vasconcelos. 2017. "Erratum: Emerging Zika Virus Infection: A Rapidly Evolving Situation." Advances in Experimental Medicine and Biology 972, no. : 141-141.
An unprecedented Ebola virus (EBOV) epidemic occurred in 2013–2016 in West Africa. Over this time the epidemic exponentially grew and moved to Europe and North America, with several imported cases and many Health Care Workers (HCW) infected. Better understanding of EBOV infection patterns in different body compartments is mandatory to develop new countermeasures, as well as to fully comprehend the pathways of human-to-human transmission. We have longitudinally explored the persistence of EBOV-specific negative sense genomic RNA (neg-RNA) and the presence of positive sense RNA (pos-RNA), including both replication intermediate (antigenomic-RNA) and messenger RNA (mRNA) molecules, in the upper and lower respiratory tract, as compared to plasma, in a HCW infected with EBOV in Sierra Leone, who was hospitalized in the high isolation facility of the National Institute for Infectious Diseases “Lazzaro Spallanzani” (INMI), Rome, Italy. We observed persistence of pos-RNA and neg-RNAs in longitudinally collected specimens of the lower respiratory tract, even after viral clearance from plasma, suggesting possible local replication. The purpose of the present study is to enhance the knowledge on the biological features of EBOV that can contribute to the human-to-human transmissibility and to develop effective intervention strategies. However, further investigation is needed in order to better understand the clinical meaning of viral replication and shedding in the respiratory tract. An unprecedented Ebola outbreak occurred in 2013–2016 in West Africa. In order to better understand EBOV infection patterns in different body compartments, we have longitudinally explored the presence of already assessed markers of ongoing EBOV replication (negative sense genomic RNA and positive sense RNA) in the upper and lower respiratory tract, as compared to plasma and other body compartments, in a Health Care Worker infected with EBOV in Sierra Leone, who was hospitalized in the high isolation facility of the National Institute for Infectious Diseases “Lazzaro Spallanzani” (INMI), Rome, Italy. The presence of total EBOV RNA and replication markers was observed in specimens of the lower respiratory tract, even after viral clearance from plasma, suggesting possible local replication. Our results contribute to the knowledge on the biological features of EBOV and shed light on the potential role of respiratory compartment in human-to-human transmissibility.
Mirella Biava; Claudia Caglioti; Licia Bordi; Concetta Castilletti; Francesca Colavita; Serena Quartu; Emanuele Nicastri; Francesco Nicola Lauria; Nicola Petrosillo; Simone Lanini; Thomas Hoenen; Gary Kobinger; Professor Sir Alimuddin Zumla; Antonino Di Caro; Giuseppe Ippolito; Maria Rosaria Capobianchi; Eleonora Lalle. Detection of Viral RNA in Tissues following Plasma Clearance from an Ebola Virus Infected Patient. PLOS Pathogens 2017, 13, e1006065 .
AMA StyleMirella Biava, Claudia Caglioti, Licia Bordi, Concetta Castilletti, Francesca Colavita, Serena Quartu, Emanuele Nicastri, Francesco Nicola Lauria, Nicola Petrosillo, Simone Lanini, Thomas Hoenen, Gary Kobinger, Professor Sir Alimuddin Zumla, Antonino Di Caro, Giuseppe Ippolito, Maria Rosaria Capobianchi, Eleonora Lalle. Detection of Viral RNA in Tissues following Plasma Clearance from an Ebola Virus Infected Patient. PLOS Pathogens. 2017; 13 (1):e1006065.
Chicago/Turabian StyleMirella Biava; Claudia Caglioti; Licia Bordi; Concetta Castilletti; Francesca Colavita; Serena Quartu; Emanuele Nicastri; Francesco Nicola Lauria; Nicola Petrosillo; Simone Lanini; Thomas Hoenen; Gary Kobinger; Professor Sir Alimuddin Zumla; Antonino Di Caro; Giuseppe Ippolito; Maria Rosaria Capobianchi; Eleonora Lalle. 2017. "Detection of Viral RNA in Tissues following Plasma Clearance from an Ebola Virus Infected Patient." PLOS Pathogens 13, no. 1: e1006065.
Zika virus is a mosquito-borne flavivirus, firstly identified in Uganda and responsible for sporadic human cases in Africa and Asia until recently, when large outbreak occurred in Pacific Ocean and the Americas. Since the main vectors during its spread outside of Africa have been Ae. albopictus and Ae. aegypti mosquitoes, which are widely distributed all over the world, there is urgent need for a coordinated response for prevention and spread of ZIKV epidemics. Despite clinical manifestation of Zika virus infection are usually mild and self limiting, there are reports suggesting, during the recent epidemic, an association of ZIKV infection with severe consequences, including fetal/newborn microcephaly, due to vertical in utero transmission, autoimmune-neurological presentations including cranial nerve dysfunction, and Guillain-Barré Syndrome in adults. The primary mode of transmission of Zika virus between humans is through the bite of an infected female mosquito of the Aedes genus, but also sexual and blood transfusion transmission may occur. Moreover, a case of non-sexual spread from one person to another has been described, indicating that we still have more to learn about Zika transmission. Biological basis for pathogenetic effects are under investigation. Laboratory diagnosis is challenging since, so far, there are no “gold standard” diagnostic tools, and the low and short viremia in the acute phase, and together with the high cross-reactivity among the members of flavivirus genus are the most challenging aspects to be overcome.
Licia Bordi; Tatjana Avšič Županc; Eleonora Lalle; Francesco Vairo; Maria Rosaria Capobianchi; Pedro Fernando Da Costa Vasconcelos. Emerging Zika Virus Infection: A Rapidly Evolving Situation. Advances in Experimental Medicine and Biology 2016, 972, 61 -86.
AMA StyleLicia Bordi, Tatjana Avšič Županc, Eleonora Lalle, Francesco Vairo, Maria Rosaria Capobianchi, Pedro Fernando Da Costa Vasconcelos. Emerging Zika Virus Infection: A Rapidly Evolving Situation. Advances in Experimental Medicine and Biology. 2016; 972 ():61-86.
Chicago/Turabian StyleLicia Bordi; Tatjana Avšič Županc; Eleonora Lalle; Francesco Vairo; Maria Rosaria Capobianchi; Pedro Fernando Da Costa Vasconcelos. 2016. "Emerging Zika Virus Infection: A Rapidly Evolving Situation." Advances in Experimental Medicine and Biology 972, no. : 61-86.