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Osteoarthritis the quality and span of life in horses. Previous studies focused on nasal cartilage as a possible source for autologous chondrocyte implantation (ACI) in cartilage defects in humans. “HOX gene-negative” nasal chondrocytes adapted articular HOX patterns after implantation into caprine joint defects and produced cartilage matrix proteins. We compared the HOX gene profile of equine chondrocytes of nasal septum, anterior and posterior fetlock to identify nasal cartilage as a potential source for ACI in horses. Cartilage was harvested from seven horses after death and derived chondrocytes were cultured in a monolayer to fourth subcultivation. HOX A3, D1, D8 and chondrocyte markers COL2 and SOX9 were analyzed with qPCR in chondrocytes of three different locations obtained during passage 0 and passage 2. HOX gene expression showed no significant differences between the locations but varied significantly between the horses. HOX genes and SOX9 remained stable during culturing. Cultured nasal chondrocytes may be a target for future research in cell-based regenerative therapies in equine osteoarthritis. The involvement of HOX genes in the high regenerative and adaptive potential of nasal chondrocytes observed in previous studies could not be confirmed.
Christiane Storch; Herbert Fuhrmann; Axel Schoeniger. HOX Gene Expressions in Cultured Articular and Nasal Equine Chondrocytes. Animals 2021, 11, 2542 .
AMA StyleChristiane Storch, Herbert Fuhrmann, Axel Schoeniger. HOX Gene Expressions in Cultured Articular and Nasal Equine Chondrocytes. Animals. 2021; 11 (9):2542.
Chicago/Turabian StyleChristiane Storch; Herbert Fuhrmann; Axel Schoeniger. 2021. "HOX Gene Expressions in Cultured Articular and Nasal Equine Chondrocytes." Animals 11, no. 9: 2542.
The knowledge of drug metabolising enzymes (DMEs) in cattle is rather limited. The capability of the bovine foetal hepatocyte-derived cell line BFH12 to serve as model for biotransformation was evaluated. Gene expression analysis of DMEs was performed by reverse transcription PCR (RT-PCR). The presence of efflux transporters was visualised by immunocytochemistry, and functional induction of cytochrome P450 (CYP) 1A was assessed by the ethoxyresorufin-O-deethylase (EROD) assay. The production of bile acids was measured by liquid chromatography-tandem mass spectrometry (LC–MS/MS). RT-PCR revealed the expression of cytochromes 1A1, 1A2, 3A4 and phase II enzymes UGT1A1, UGT1A6 and GSTM1. Immunofluorescence demonstrated efflux transporters ABCG2 and ABCC1. The EROD assay revealed a dose-dependent CYP1A induction after treatment with benzo[a]pyrene (BP). LC–MS/MS analysis of cell culture supernatants showed the production of bile acids including taurocholic acid, tauro-chenodeoxycholic acid, taurodeoxycholic acid and taurolithocholic acid. The results strongly suggest the applicability of the cell line BFH12 for subsequent experiments in the emerging field of bovine biotransformation.
Alexander Gleich; Bastian Kaiser; Walther Honscha; Herbert Fuhrmann; Axel Schoeniger. Evaluation of the hepatocyte-derived cell line BFH12 as an in vitro model for bovine biotransformation. Cytotechnology 2019, 71, 231 -244.
AMA StyleAlexander Gleich, Bastian Kaiser, Walther Honscha, Herbert Fuhrmann, Axel Schoeniger. Evaluation of the hepatocyte-derived cell line BFH12 as an in vitro model for bovine biotransformation. Cytotechnology. 2019; 71 (1):231-244.
Chicago/Turabian StyleAlexander Gleich; Bastian Kaiser; Walther Honscha; Herbert Fuhrmann; Axel Schoeniger. 2019. "Evaluation of the hepatocyte-derived cell line BFH12 as an in vitro model for bovine biotransformation." Cytotechnology 71, no. 1: 231-244.