Mung bean, a legume, is sensitive to abiotic stresses at different growth stages, and its yield potential is affected by drought and high-temperature stress at the sensitive stage. Melatonin is a multifunctional hormone that plays a vital role in plant stress defense mechanisms. This study aimed to evaluate the efficiency of melatonin under individual and combined drought and high-temperature stress in mung bean. An experiment was laid out with five treatments, including an exogenous application of 100 µM melatonin as a seed treatment, foliar spray, and a combination of both seed treatment and foliar spray, as well as absolute control (ambient condition) and control (stress without melatonin treatment). Stresses were imposed during the mung bean’s reproductive stage (31–40 DAS) for ten days. Results revealed that drought and high-temperature stress significantly decreased chlorophyll index, Fv/Fm ratio, photosynthetic rate, stomatal conductance, and transpiration rate through increased reactive oxygen species (ROS) production. Foliar application of melatonin at 100 µM concentration enhanced the activity of antioxidant enzymes such as superoxide dismutase, catalase, and ascorbate peroxidase and the concentration of metabolites involved in osmoregulation and ion homeostasis; thereby, it improves physiological and yield-related traits in mung bean under individual and combined stress at the reproductive stage.

Insecticide applications are typically being carried out with traditional manual spraying equipment in redgram, which leads to inadequate control of insects due to higher crop height. The modern deployment of tractor-drawn spray machines causes serious damage to the crop. In this connection, unmanned aerial vehicle (UAV) spray technology has great potential for precise insecticide application in redgram crops. One of the important machine parameters influencing droplet deposition and drift characteristics in UAV sprayers is downwash airflow generated by a multi-rotor propeller. A field experiment was carried out at the redgram research field (N11.01, E76.92), Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, during 2021–2022 to study the spray drift and deposition characteristics of an autonomous UAV sprayer. The Imidacloprid (a.i. 17.8SL) insecticide mixed with water in a ratio of 1 mL per liter was sprayed with a UAV sprayer. Water-sensitive paper samples were kept at upper, middle, and bottom positions on the leaves, and data were analyzed for the spray droplet size, deposition rate, droplet density, and area coverage both in target and non-target areas using Spray Deposit Scanner software. UAV spray droplet deposition rate (2.93 ± 0.17, 2.01 ± 0.08, and 2.21 ± 0.162.38 μL cm⁻²), droplet density (47 ± 4.04, 53 ± 3.61, and 52 ± 8.74 droplets cm⁻²), and area coverage (15.72 ± 0.39, 16.60 ± 0.71, and 14.99 ± 0.39%) were highest in the upper layer as compared to the middle layer (droplet deposition rate: 1.21 ± 0.08, 1.07 ± 0.03, and 0.77 ± 0.02 μL cm⁻²; droplet density: 42 ± 2.52, 43 ± 8.50, and 38 ± 2.52 droplets cm⁻²; area coverage: 10.95 ± 0.81, 11.22 ± 0.56, and 8.57 ± 0.44%) and bottom layer (droplet deposition rate: 0.41 ± 0.06, 0.35 ± 0.03, and 0.33 ± 0.03 μL cm⁻²; droplet density: 22 ± 4.36, 17 ± 3.51, and 19 ± 4.51 droplets cm⁻²; area coverage: 2.78 ± 0.29, 2.95 ± 0.45, and 2.46 ± 0.20%, respectively). In the spray drift test, there was a higher droplet deposition rate (1.63 ± 0.09, 1.93 ± 0.05, and 1.82 ± 0.06 μL cm⁻²), area coverage (14.40 ± 0.07, 17.54 ± 0.36, and 16.42 ± 0.30%), and droplet density (46 ± 3.61, 54 ± 2.08, and 45 ± 3.21 No’s cm⁻²) in the target area as compared to the non-target area (droplet deposition rate: 0.88 ± 0.02, 0.46 ± 0.03, 0.22 ± 0.05, and 0.00 μL cm⁻²; droplet density: 23 ± 1.53, 11 ± 2.08, 6 ± 1.53, and 0.00 droplets cm⁻²; area coverage: 7.58 ± 0.34, 4.41 ± 0.19, 2.16 ± 0.05, and 0.00%, respectively), which may have been due to the downwash airflow produced by the multi-rotor propeller of the UAV sprayer. Finally, the UAV-based spraying technology results showed that the downwash air produced by the six-rotor propeller improved the penetrability of insecticide to crop leaves and led to a higher droplet deposition rate, droplet density, area coverage, and droplet penetrability on the upper layer, middle layer, and bottom layer of the plants.

Biostimulants are innovative organic tools, which promote the growth, plant development, production, and quality of various crops without harming the environment; however, the effects of biostimulants on the production of tomato needed to be explored further under open field conditions. Based on this view, this study’s objective was to assess the impact of Kendal Root, a biostimulant-containing seaweed, Ascophyllum nodosum, and plant extracts on the phytomorpho-physiological, yield, and quality of tomato. Three doses of Kendal Root (2.5, 5.0, and 10 L ha⁻¹) were given as soil drenching, and the results were compared with control. Generally, the Kendal Root treatments positively improved the growth, physiological, yield, and quality attributes of tomato. However, among the three different concentrations, Kendal Root 5.0 L ha⁻¹ significantly improved the plant growth and physiological aspects of tomato, such as plant height, leaf area, shoot and root dry weight, SPAD value, and gas exchange parameters. Considering the yield traits, the Kendal Root 5.0 L ha⁻¹ application significantly improved the tomato fruit number, yield per plant, and yield per hectare. Conversely, flower number per plant and average fruit weight was not remarkably improved by Kendal Root 5.0 L ha⁻¹. Moreover, Kendal Root 5.0 L ha⁻¹ positively improved the quality traits of tomato, including total soluble solids, ascorbic acid content, lycopene, and total sugars than the titratable acidity content of tomato fruits. Hence, the integration of Kendal Root biostimulant in tomato production could be an effective way to boost plant growth, production, and quality of tomato.

In tomato (Lycopersicon esculentum L.), the effects of combined drought (D) and high temperature (HT) stress during the flowering stage had not been studied in detail. Therefore, this study was conducted with an objective of quantifying the effects of foliar spray of melatonin under individual and combined drought and HT stress. At flowering stage, D stress was imposed through withholding irrigation, while HT stress was imposed through exposing the plants to ambient temperature (AT) along with an increase of +5 °C. Under D + HT, plants were first subjected to drought followed by a + 5 °C increase in AT. The duration of individual or combined stress was ten days. At 80% available soil moisture, 100 µM melatonin was sprayed on D, HT, or D + HT treated plants. Among the stresses, D + HT stress increased the thylakoid membrane damage and decreased the photosynthetic rate and fruit yield more than D or HT stress. Foliar spray of 100 µM melatonin produced decreased thylakoid membrane damage [D: 31%, HT: 26%, and D + HT: 18%] and increased antioxidant enzyme, viz., superoxide dismutase, catalase, peroxidase, ascorbate peroxidase, and glutathione reductase, activity over stress-control plants. The photosynthetic rate [D: 24%, HT: 22%, and D + HT: 19%] and fruit yield [D: 32%, HT: 23%, and D + HT: 16%] were increased over stress-control plants. Hence, it is evident that the increased photosynthetic rate and fruit yield in D + HT and 100 µM melatonin-sprayed plants may be associated with an increased antioxidant defense system. Melatonin as a novel biostimulator has a great potential in scavenging free radicals through increased antioxidant activity, which shields the photosynthetic membrane from damage and therefore helps in stress mitigation.

Maize (Zea mays L.) is an important crop grown for food, feed, and energy. In general, maize yield is decreased due to drought stress during the reproductive stages, and, hence, it is critical to improve the grain yield under drought. A field experiment was conducted with a split-plot design. The main factor was the irrigation regime viz. well-irrigated conditions and withholding irrigation from tasseling to grain filling for 21 days. The subplots include six treatments, namely, (i) the control (water spray), (ii) zinc oxide @ 100 ppm, (iii) manganese oxide @ 20 ppm, (iv) nZnO @ 100 ppm + nMnO @ 20 ppm, (v) Tamil Nadu Agricultural University (TNAU) Nano Revive @ 1.0%, and (vi) zinc sulfate 0.25% + manganese sulfate 0.25%. During drought stress, the anthesis–silking interval (ASI), chlorophyll a and b content, proline, starch, and carbohydrate fractions were recorded. At harvest, the grain-filling rate and duration, per cent green leaf area, and yield traits were recorded. Drought stress increased the proline (38.1%) and anthesis–silking interval (0.45 d) over the irrigated condition. However, the foliar application of ZnO (100 ppm) and nMnO (20 ppm) lowered the ASI and increased the green leaf area, leaf chlorophyll index, and proline content over water spray. The seed-filling rate (17%), seed-filling duration (11%), and seed yield (19%) decreased under drought. Nevertheless, the seed-filling rate (90%), seed-filling duration (13%), and seed yield (52%) were increased by the foliar spraying of nZnO (100 ppm) and nMnO (20 ppm) over water spray. These findings suggest that nZnO and nMnO significantly improve the grain yield of maize under drought stress conditions.

Melatonin (MT), a naturally occurring compound, is found in various species worldwide. In 1958, it was first identified in the pineal gland of dairy cows. MT is an “old friend” but a “new compound” for plant biology. It brings experts and research minds from the broad field of plant sciences due to its considerable influence on plant systems. The MT production process in plants and animals is distinct, where it has been expressed explicitly in chloroplasts and mitochondria in plants. Tryptophan acts as the precursor for the formation of phyto-melatonin, along with intermediates including tryptamine, serotonin, N-acetyl serotonin, and 5-methoxy tryptamine. It plays a vital role in growth phases such as the seed germination and seedling growth of crop plants. MT significantly impacts the gas exchange, thereby improving physio-chemical functions in plant systems. During stress, the excessive generation and accumulation of reactive oxygen species (ROS) causes protein oxidation, lipid peroxidation, nucleic acid damage, and enzyme inhibition. Because it directly acts as an antioxidant compound, it awakens the plant antioxidant defense system during stress and reduces the production of ROS, which results in decreasing cellular oxidative damage. MT can enhance plant growth and development in response to various abiotic stresses such as drought, salinity, high temperature, flooding, and heavy metals by regulating the antioxidant mechanism of plants. However, these reactions differ significantly from crop to crop and are based on the level and kind of stress. The role of MT in the physiological functions of plants towards plant growth and development, tolerance towards various abiotic stresses, and approaches for enhancing the endogenous MT in plant systems are broadly reviewed and it is suggested that MT is a steering compound in directing major physiological functions of plants under the changing climate in future.