In this study, the Cutaneotrichosporon dermatis strain M503 was isolated and could efficiently degrade tetracycline, doxycycline, and chlorotetracyline. The characteristics of tetracycline degradation were investigated under a broad range of cultural conditions. Response surface methodology (RSM) predicted that the highest degradation rate of tetracycline could be obtained under the following conditions: 39.69 °C, pH of 8.79, and inoculum dose of 4.0% (v/v, ~3.5 × 10⁶ cells/mL in the medium). In accordance with the five identified degradation products of tetracycline, two putative degradation pathways, which included the shedding of methyl and amino groups, were proposed. Moreover, the well diffusion method showed that the strain of M503 decreases the antibacterial potency of tetracycline, doxycycline, and chlorotetracycline. These findings proposed a putative mechanism of tetracycline degradation by a fungus strain and contributed to the estimation of the fate of tetracycline in the aquatic environment.

Phenolic inhibitors generated during alkaline pretreatment of lignocellulosic biomasses significantly hinder bacterial growth and subsequent biofuel and biochemical production. Water rinsing is an efficient method for removing these compounds. Nevertheless, this method often generates a great amount of wastewater, and leads to the loss of solid fiber particles and fermentable sugars. Kurthia huakuii LAM0618^T, a recently identified microorganism, was herein shown to be able to efficiently transform phenolic compounds (syringaldehyde, hydroxybenzaldehyde, and vanillin) into less toxic acids. Taking advantage of these properties, a biodetoxification method was established by inoculating K. huakuii LAM0618^T into the NH₃/H₂O₂-pretreated unwashed corn stover to degrade phenolic inhibitors and weak acids generated during the pretreatment. Subsequently, 33.47 and 17.91 g/L lactic acid was produced by Bacillus coagulans LA204 at 50 °C through simultaneous saccharification and fermentation (SSF) from 8% (w/w) of NH₃/H₂O₂-pretreated corn stover with or without K. huakuii LAM0618^T-biodetoxification, indicating biodetoxification significantly increased lactic acid titer and yield. Importantly, using 15% (w/w) of the NH₃/H₂O₂-pretreated K. huakuii LAM0618^T-biodetoxified corn stover as a substrate through fed-batch simultaneous saccharification and fermentation, high titer and high yield of lactic acid (84.49 g/L and 0.56 g/g corn stover, respectively, with a productivity of 0.88 g/L/h) were produced by Bacillus coagulans LA204. Therefore, this study reported the first study on biodetoxification of alkaline-pretreated lignocellulosic material, and this biodetoxification method could replace water rinsing for removal of phenolic inhibitors and applied in biofuel and biochemical production using the alkaline-pretreated lignocellulosic bioresources.

Strain Q11^T of an irregular coccoid Gram-positive bacterium, aerobic and non-motile, was isolated from Meconopsis integrifolia seeds. Strain Q11^T grew optimally in 1% (w/v) NaCl, pH 7, at 30 °C. Strain Q11^T is most closely related to Flexivirga, as evidenced by 16S rRNA gene analysis, and shares the highest similarity with Flexivirga aerilata ID2601S^T (99.24%). Based on genome sequence analysis, the average nucleotide identity and digital DNA–DNA hybridization values of strains Q11^T and D2601S^T were 88.82% and 36.20%, respectively. Additionally, strain Q11^T showed the abilities of nitrogen fixation and indole acetic acid production and was shown to promote maize growth under laboratory conditions. Its genome contains antibiotic resistance genes (the vanY gene in the vanB cluster and the vanW gene in the vanI cluster) and extreme environment tolerance genes (ectoine biosynthetic gene cluster). Shotgun proteomics also detected antibiotic resistance proteins (class A beta-lactamases, D-alanine ligase family proteins) and proteins that improve plant cold tolerance (multispecies cold shock proteins). Strain Q11^T was determined to be a novel species of the genus Flexivirga, for which the name Flexivirga meconopsidis sp. nov. is proposed. The strain type is Q11^T (GDMCC 1.3002^T = JCM 36020 ^T).

Continuous cropping is the main form of cultivation in Chinese agriculture. The bacterial community plays an important role in maintaining the healthy growth of plants. However, there are few reports on the composition and dynamics of the bacterial community structure under continuous cropping of Lonicera japonica Thunb. High-throughput sequencing was used to monitor the variation in the soil bacterial community structure of different monocropping years of Lonicera japonica Thunb., as well as the correlation between soil characteristics and bacterial community. Meanwhile, antagonistic bacteria for Fusarium oxysporum pathogens were isolated and functionally verified by culture-dependent techniques and pot experiments. Bacterial community diversity and structure changed significantly with the increase in the years of Lonicera japonica Thunb. succession. However, some beneficial bacteria, such as Bacillus and Nitrosospira, were gradually depleted. The complexity of the bacteria co-occurrence networks decreased with increasing years of cropping. FAPROTAX-based functional prediction showed that the abundance of genes related to carbon, nitrogen, sulfur metabolism and chitinlysis were reduced with the extended crop succession. Furthermore, the three Bacillus strains that were strongly antagonistic toward Fusarium oxysporum and the pot experiment demonstrated they significantly promoted Lonicera japonica Thunb. growth. Our research provides theoretical support for the development of microbial fertilizers that are beneficial to plants.

A Gram-stain positive, aerobic, rod-shaped bacterium, designated BYT-33-1 ^T , was isolated from a typical sandy soil in Xinjiang, PR China. This isolate grew well at 20–40 °C, pH 6.0–10.0 and 0–3.0% (w/v) NaCl, with optimal growth at 30 °C, pH 7.0 and 0.5% NaCl, respectively. Phylogenetic analysis of 16S rRNA gene sequences indicated that BYT-33-1 ^T shared the highest sequence similarities with Nocardioides nitrophenolicus NSP 41 ^T (98.4%) and Nocardioides kongjuensis A2-4 ^T (98.3%). The draft genome was 5,327,936 bp, consisting of 24 contigs, with a G+C content of 72.4 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain BYT-33-1 ^T and its closely related type strains of genus Nocardioides were 88.1–80.5% and 35.0–23.5%, respectively. The predominant menaquinone was MK-8(H ₄ ), and C _18:0 10-methyl and iso-C _16:0 constituted the major cellular fatty acids. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, one unidentified aminolipid (AL) and three unidentified phospholipids. The ll -diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. In addition, strain BYT-33-1 ^T exhibits hexadecane degradation ability, suggesting its potential application value in alkane bioremediation of contaminated environments. Based on chemotaxonomic, phylogenetic, phenotypic and genomic analyses, we propose a novel species, named Nocardioides segetis sp. nov., with the type strain BYT-33-1 ^T (=CCTCC AA 2024001 ^T =KCTC 59242 ^T ).