In this study, the Cutaneotrichosporon dermatis strain M503 was isolated and could efficiently degrade tetracycline, doxycycline, and chlorotetracyline. The characteristics of tetracycline degradation were investigated under a broad range of cultural conditions. Response surface methodology (RSM) predicted that the highest degradation rate of tetracycline could be obtained under the following conditions: 39.69 °C, pH of 8.79, and inoculum dose of 4.0% (v/v, ~3.5 × 10⁶ cells/mL in the medium). In accordance with the five identified degradation products of tetracycline, two putative degradation pathways, which included the shedding of methyl and amino groups, were proposed. Moreover, the well diffusion method showed that the strain of M503 decreases the antibacterial potency of tetracycline, doxycycline, and chlorotetracycline. These findings proposed a putative mechanism of tetracycline degradation by a fungus strain and contributed to the estimation of the fate of tetracycline in the aquatic environment.

A non-motile, Gram-staining-negative, orange-pigmented bacterium called herbae pc1-10^T was discovered in Tibet in the soil around Pyrola calliantha H. Andres’ roots. The isolate thrived in the temperature range of 10–30 °C (optimal, 25 °C), pH range of 5.0–9.0 (optimum, pH = 6.0), and the NaCl concentration range of 0–1.8% (optimal, 0%). The DNA G+C content of the novel strain was 37.94 mol%. It showed the function of dissolving organophosphorus, acquiring iron from the environment by siderophore and producing indole acetic acid. Moreover, the genome of strain herbae pc1-10^T harbors two antibiotic resistance genes (IND-4 and AdeF) encoding a β-lactamase, and the membrane fusion protein of the multidrug efflux complex AdeFGH; antibiotic-resistance-related proteins were detected using the Shotgun proteomics technology. The OrthoANIu values between strains Chryseobacterium herbae pc1-10^T; Chryseobacterium oleae CT348^T; Chryseobacterium kwangjuense KJ1R5^T; and Chryseobacterium vrystaatense R-23566^T were 90.94%, 82.96%, and 85.19%, respectively. The in silico DDH values between strains herbae pc1-10^T; C. oleae CT348^T; C. kwangjuense KJ1R5^T; and C. vrystaatense R-23566^T were 41.7%, 26.6%, and 29.7%, respectively. Chryseobacterium oleae, Chryseobacterium vrystaatense, and Chryseobacterium kwangjuense, which had 16S rRNA gene sequence similarity scores of 97.80%, 97.52%, and 96.75%, respectively, were its closest phylogenetic relatives. Chryseobacterium herbae sp. nov. is proposed as the designation for the strain herbae pc1-10^T (=GDMCC 1.3255 = JCM 35711), which represented a type species based on genotypic and morphological characteristics. This study provides deep knowledge of a Chryseobacterium herbae characteristic description and urges the need for further genomic studies on microorganisms living in alpine ecosystems, especially around medicinal plants.

A Gram-stain positive, aerobic, rod-shaped bacterium, designated BYT-33-1 ^T , was isolated from a typical sandy soil in Xinjiang, PR China. This isolate grew well at 20–40 °C, pH 6.0–10.0 and 0–3.0% (w/v) NaCl, with optimal growth at 30 °C, pH 7.0 and 0.5% NaCl, respectively. Phylogenetic analysis of 16S rRNA gene sequences indicated that BYT-33-1 ^T shared the highest sequence similarities with Nocardioides nitrophenolicus NSP 41 ^T (98.4%) and Nocardioides kongjuensis A2-4 ^T (98.3%). The draft genome was 5,327,936 bp, consisting of 24 contigs, with a G+C content of 72.4 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain BYT-33-1 ^T and its closely related type strains of genus Nocardioides were 88.1–80.5% and 35.0–23.5%, respectively. The predominant menaquinone was MK-8(H ₄ ), and C _18:0 10-methyl and iso-C _16:0 constituted the major cellular fatty acids. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, one unidentified aminolipid (AL) and three unidentified phospholipids. The ll -diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. In addition, strain BYT-33-1 ^T exhibits hexadecane degradation ability, suggesting its potential application value in alkane bioremediation of contaminated environments. Based on chemotaxonomic, phylogenetic, phenotypic and genomic analyses, we propose a novel species, named Nocardioides segetis sp. nov., with the type strain BYT-33-1 ^T (=CCTCC AA 2024001 ^T =KCTC 59242 ^T ).