Medical article медицинская статья

A major problem in psychiatric research is a deficit of relevant cell material of neuronal origin, especially in large quantities from living individuals. One of the promising options is cells from the olfactory neuroepithelium, which contains neuronal progenitors that ensure the regeneration of olfactory receptors. These cells are easy to obtain with nasal biopsies and it is possible to grow and cultivate them in vitro. In this work, we used RNAseq expression profiling and immunofluorescence microscopy to characterise neurospheres-derived cells (NDC), that simply and reliably grow from neurospheres (NS) obtained from nasal biopsies. We utilized differential expression analysis to explore the molecular changes that occur during transition from NS to NDC. We found that processes associated with neuronal and vascular cells are downregulated in NDC. A comparison with public transcriptomes revealed a depletion of neuronal and glial components in NDC. We also discovered that NDC have several metabolic features specific to neuronal progenitors treated with the fungicide maneb. Thus, while NDC retain some neuronal/glial identity, additional protocol alterations are needed to use NDC for mass sample collection in psychiatric research.

Medical article медицинская статья

Medical article медицинская статья

Epistaxis is a common condition among the world’s population that in 6-10% of cases requires medical care. The development of local impact methods to stop epistaxis by specialists of ambulance and emergency medical teams determines the medical advisability and economic efficacy of this care. Objective. To analyze the existing methods of epistaxis stopping and the prospects of their optimization for the medical care provision at the prehospital stage by ambulance and emergency medical teams. Material and methods. Domestic and foreign scientific sources presented in PubMed and eLibrary for the last 20 years were analyzed by keywords: gauze tamponade, prehospital stage, nasal tamponade, ambulance medical care, emergency medical care. Results. The preferred characteristics of anterior nasal tamponade which should be modified have been determined to improve the efficacy of medical care provision for patients with epistaxis at the prehospital stage. Conclusion. The analysis of existing methods for epistaxis stopping allowed to determine the preferred characteristics of anterior nasal tamponade modification to optimize the provision of ambulance and emergency medical care at the prehospital stage. These features are rapid and atraumatic intranasal introduction of a hydrotampon, providing a combined effect of controlled compression on vessels of the nasal cavity mucosa, which does not require special skills and tools for its installation.