Background and Aim: Dairy ration formulations should consider the synchronization of the rumen degradable protein (RDP) to rumen undegradable protein (RUP) ratio (RDPR) with non-fiber carbohydrate (NFC) to achieve optimum microbial protein synthesis (MPS), reduce feed costs, and reduce N excretion to the environment. This study aimed to investigate the effect of RDPR and NFC synchronization on in vitro digestibility, fermentability, and MPS. Materials and Methods: The experiment used a 3×3 factorial randomized block design with four replications. The first factor was RDPR (RDPR1=50:50; RDPR2=55:45; RDPR3=60:40) and the second factor was NFC levels (NFC1=30%, NFC2=35%, NFC3=40%). The experimental diets were evaluated using a two-stage in vitro method. The examined parameters included rumen pH, NH3 concentration, total volatile fatty acid (VFA) concentration, the molar proportion of VFAs, rumen microbes (protozoa and total bacteria population), and MPS. Data were analyzed using ANOVA, followed by the Duncan test. Results: The results show that neither RDPR nor NFC affected rumen pH, NH3, total VFA, and the rumen microbe population. The interaction between RDPR and NFC affected the molar proportion of acetate, iso-butyrate, and n-valerate. The combination of RDPR1 and NFC1 produced a lower molar proportion of acetate (49.73%) than the other treatment combinations (>54%). The acetate to propionate ratio was influenced by the NFC levels, in which NFC2 and NFC3 produced the highest ratio (p<0.05). MPS was affected by RDPR and NFC, but not by their interaction. Treatments NFC2 and RDPR3 produced the highest MPS. NFC affected the dry matter and organic matter digestibility (DMD and OMD), with treatment NFC3 resulting in the highest DMD and OMD. Conclusion: The combination of a 60:40 RDPR with 35% NFC resulted in the best synchronization of protein and energy available for MPS and digestion activity in the rumen.

Model Pendugaan Total Digestible Nutrient (TDN) pada Hijauan Pakan Tropis menggunakan Komposisi Nutrien

Supplementation of oil rich in unsaturated fatty acids (FAs) such as canola, soybean, and palm oils improved the quality of milk fatty acids. However, the unprotected unsaturated oil might impair rumen fermentation, feed, and fiber digestibility. A study was conducted to determine the best type of oil supplementation (factor A) including canola (A1), soybean (A2), or palm (A3) and level oil supplementation (factor B) including B0 = 0%, B1 = 1%, B2 = 2% or B3 = 3%) on the in-vitro feed fermentation and digestibility. The study used a 3 x 4 factorial block design. Two-stages were used to measure the pH, ammonia (NH3), volatile fatty acids (VFAs), protozoal number, dry matter (DMD), organic matter (OMD), neutral detergent fiber (NDFD), and acid detergent fiber (ADFD) digestibility. The results showed that oil type did not significantly influence the fermentability (pH, NH3, VFAs, and protozoa) and feed's digestibility (DMD, OMD, NDFD, and ADFD) but oil level influence the fermentability and digestibility significantly. In addition, an increase above 1% in oil levels reduced protein fermentability, protozoal number, DMD, and OMD, but increased VFA. It is concluded that the addition of unprotected canola, soybean, or palm oil in dairy cattle ration could be applied in a concentration not more than 1%.

Geographical position and feed quality of farms in highland and lowland, particularly in the tropical region, cause the differences in milk quality. One of the factors that influenced milk quality is the fatty acid (FA) profile of milk. The objective of this study is to compare the FA profiles of milk produced by farms in highland and lowland regions. Milk sampling was done by random purposive method at two regions which have different altitudes. Milk sampling was conducted with 28 farmers whose cows were fed botanically diverse forages and with commercial mix concentrate of dairy cows. The FA profiles of the milk samples were analyzed using Gas Chromatography (GC-7820A/G4350B, Agilent Technologies). Individual FAME was identified by comparison to standard mixture Supelco 37 Component FAME Mix and standard CLA (O5507, Sigma-Aldrich). The FA profiles were statistically analyzed for normality test and t-test carried out by SPSS ver.22. There were nine significantly different FA profiles consisting of C6:0, C10:0, C12:0, C14:0, C16:0, C18:0, C18:1-trans9, C18:2-trans, C18:2-cis, and C18:2-CLA. The milk from the lowland region has higher PUFA content, especially CLA content, and also has lower SFA content. The conclusion is milk from highland and lowland regions consists of different FA profiles.

The purpose of this study was to compare acid and base catalyst for the extraction of Fatty acids (FA) and conjugated linoleic acid from milk product. Lipid extraction from milk by using acid catalyst were carried out in H₂SO₄/methanol solvents. Methylation was performed for 2 h at 80°C, and FA methyl esters were recovered for chromatographic analysis by the addition of isooctane. On the other hand, the extraction using base catalysts was conducted by AOAC 989.05 (2012) official method, followed by AOAC 969.33 (2012) for methylation. Extraction process by acid catalyst shown better performance compared to base catalyst due to it could be produced higher recoveries. The advantage of extraction method by using acid-catalyst allowed the measurement of high number of samples. Moreover, it can be reduced the sample manipulation and consequently reduce sample loss and contamination. In conclusion, the acid-catalyst method was a simple and rapid method, low cost, and achieves good results for Fatty acids (FA) and conjugated linoleic acid extraction from milk product.

This study aimed to compare calcium oxide (CaO) and calcium chloride (CaCl₂) as calcium sources for coconut fatty acid distillate (CFAD) calcium soap (Ca-soap) production and to evaluate the supplementation ratios of unprotected and protected CFAD in dairy rations to optimize rumen function. This research included two steps: (1) assessing the protection strength of Ca-soap made with CaO and CaCl₂ at mole ratios of Ca to CFAD of 1, 1.5, 2, and 2.5; (2) evaluating CFAD supplementation in an in vitro dairy ration study using a 5 × 4 randomized factorial block design. Factor A compared unprotected and protected CFAD ratios of A1 = 100:0, A2 = 75:25, A3 = 50:50, A4 = 25:75, and A5 = 0:100, and factor B compared supplementation levels of B1 = 0%, B2 = 1%, B3 = 2%, and B4 = 3%. CaCl₂ at a 2.5-mole ratio to CFAD produced the lowest acid value and the carboxylic acid (C=O) chemical bond. Complete protection (0:100) exhibited the highest densities of Bacteroides and nutrient digestibility (p < 0.05) without significantly affecting rumen fermentability (p > 0.05). Higher CFAD levels significantly reduced methanogens and protozoa (p < 0.05) without significantly affecting estimated methane production. In conclusion, CaCl₂ at a 2.5-mole ratio to CFAD provided the best protection, and its complete protection in CFAD supplementation optimized rumen function.