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Prof. Mohamed Mohamed Soliman
Clinical Laboratory Scineces, Turabah University College, Taif University, Saudi Arabia

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0 molecular biomarkers
0 Molecular Toxicology
0 Antioxidant and anti-inflammatory compounds
0 Molecular and Cellular Pharmacology
0 Herbal medicinal product

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Molecular and Cellular Pharmacology
Antioxidant and anti-inflammatory compounds

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Original article
Published: 30 August 2021 in Veterinary Research Communications
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Male broiler chicks (135 Indian River chicks (IR) and 135 Cobb chicks; n = 270) were weighed, wing banded, and distributed randomly into three iso-energetic and iso-nitrogenous diet groups for each breed (triplicate design, 45 bird/group, 15 bird/replicate). The chicks were fed the diets with levels of 0, 4, or 6% sun-dried tomato pomace (SDTP), respectively, for 42 consecutive days to determine the effect of consuming different levels of SDTP on growth performance, economic efficiency, meat quality, and gene expression in IR and Cobb broiler chickens. The inclusion of up to 6% SDTP in the diet of IR or Cobb chickens had no negative impact on growth performance parameters. Chickens from both the IR and Cobb breeds fed a diet containing 4% or 6% SDTP consumed more feed than those fed a diet containing 0% SDTP. Concomitantly, the groups fed a 6% SDTP diet of IR breed incurred a significantly higher feed cost, total variable cost (TVC), and total cost (TC). The inclusion of up to 6% SDTP in the feed of both breeds resulted in a non-significant increase in return parameters. The ultimate pH decreased as the SDTP concentration increased, with no significant differences in water holding capacity (WHC) or drip loss (48 h). No alteration in the mRNA expression of hepatic growth hormone receptor gene (GHR) or insulin like growth factor-1 (IGF-1) was found among the treatments for either the IR or Cobb breeds. Thus, up to 6% SDTP can be added to the diet of IR and Cobb broiler chickens without any adverse effects on the examined parameters.

ACS Style

Liza S. Mohammed; Eman A. Sallam; Shimaa N. Edris; Olla A. Khalifa; Mohamed Mohamed Soliman; Seham F. Shehata. Growth performance, economic efficiency, meat quality, and gene expression in two broiler breeds fed different levels of tomato pomace. Veterinary Research Communications 2021, 1 -17.

AMA Style

Liza S. Mohammed, Eman A. Sallam, Shimaa N. Edris, Olla A. Khalifa, Mohamed Mohamed Soliman, Seham F. Shehata. Growth performance, economic efficiency, meat quality, and gene expression in two broiler breeds fed different levels of tomato pomace. Veterinary Research Communications. 2021; ():1-17.

Chicago/Turabian Style

Liza S. Mohammed; Eman A. Sallam; Shimaa N. Edris; Olla A. Khalifa; Mohamed Mohamed Soliman; Seham F. Shehata. 2021. "Growth performance, economic efficiency, meat quality, and gene expression in two broiler breeds fed different levels of tomato pomace." Veterinary Research Communications , no. : 1-17.

Journal article
Published: 19 August 2021 in Animals
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Healthy, weaned, coccidial-free male rabbits from two breeds (New Zealand white (NZ) and V-line (VL)) were divided into 10 equal groups (5 groups each for NZ and VL) (3 replicates/group, 6 rabbits/replicate, 18 rabbits/group). All rabbits were inoculated with 5 × 104Eimeria spp. oocysts (E. intestinalis (67%), E. magna (22%), and E. media (11%)) except for the rabbits in the first group (G1), which were inoculated with a sterile solution and served as a negative control. The remaining four groups were treated as follows: G2, no treatment/positive control, G3, treated with neem leaf extract, G4, treated with pomegranate peel extract (PPE), and G5, treated with a combination of neem leaf extract and PPE. For both breeds, our results showed that the use of neem leaf and/or pomegranate peel extract resulted in improved growth performance, with a significant improvement in relative feed conversion ratio (FCR) compared to the positive control groups, which recorded the worst values, as well as a significant (p ≤ 0.05) reduction in mean oocyst count compared to the positive control groups. We also observed downregulation of mRNA levels of IL-1βα, IL6, and TNF-α in the herbal treatment groups compared with the mRNA levels of these genes in the positive control groups. Herbal treatment with neem leaf and/or pomegranate peel extracts had positive effects on the NZ and VL rabbits experimentally infected with mixed Eimeria species, as evidenced by their healthy appearance, good appetite, no mortalities, an anticoccidial index > 120, and a significantly higher total return and net profit when compared to the positive control groups of both breeds. In NZ rabbits, the treatment with neem leaf extract alone (G3) or in combination with PPE (G5) recorded the most efficient economic anticoccidial activity.

ACS Style

Liza S. Mohammed; Eman A. Sallam; Sawsan S. El Basuni; Amany S. Eldiarby; Mohamed Mohamed Soliman; Salama Mostafa Aboelenin; Seham F. Shehata. Ameliorative Effect of Neem Leaf and Pomegranate Peel Extracts in Coccidial Infections in New Zealand and V-Line Rabbits: Performance, Intestinal Health, Oocyst Shedding, Carcass Traits, and Effect on Economic Measures. Animals 2021, 11, 2441 .

AMA Style

Liza S. Mohammed, Eman A. Sallam, Sawsan S. El Basuni, Amany S. Eldiarby, Mohamed Mohamed Soliman, Salama Mostafa Aboelenin, Seham F. Shehata. Ameliorative Effect of Neem Leaf and Pomegranate Peel Extracts in Coccidial Infections in New Zealand and V-Line Rabbits: Performance, Intestinal Health, Oocyst Shedding, Carcass Traits, and Effect on Economic Measures. Animals. 2021; 11 (8):2441.

Chicago/Turabian Style

Liza S. Mohammed; Eman A. Sallam; Sawsan S. El Basuni; Amany S. Eldiarby; Mohamed Mohamed Soliman; Salama Mostafa Aboelenin; Seham F. Shehata. 2021. "Ameliorative Effect of Neem Leaf and Pomegranate Peel Extracts in Coccidial Infections in New Zealand and V-Line Rabbits: Performance, Intestinal Health, Oocyst Shedding, Carcass Traits, and Effect on Economic Measures." Animals 11, no. 8: 2441.

Journal article
Published: 12 August 2021 in Pharmaceutics
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The present study was conducted to evaluate the analgesic potential of the new triamilide macrolide antibiotic, tulathromycin, at 20 and 40 mg/kg of body weight (BW), subcutaneously against acute pain in mice. Acute pain was induced either chemically (using acetic acid-induced writhing and formalin-induced pain tests) or thermally (using hot-plate, and tail-flick tests). In the acetic acid-induced writhing test, tulathromycin induced a dose-dependent and significant decrease in the number of writhes compared with the control group. In the late phase of the formalin test, a significant decline in hind paw licking time compared with the control group was observed. In the hot-plate and tail-flick tests, tulathromycin caused a dose-dependent and significant prolongation of latency of nociceptive response to heat stimuli, compared with the control group. These findings may indicate that tulathromycin possesses significant peripheral and central analgesic potentials that may be valuable in symptomatic relief of pain, in addition to its well-established antibacterial effect.

ACS Style

Mohamed Elbadawy; Amira Abugomaa; Hussein M. El-Husseiny; Ahmed S. Mandour; Mohamed M. Abdel-Daim; Salama Mostafa Aboelenin; Mohamed Mohamed Soliman; Amany El-Mleeh. The Anti-Nociceptive Potential of Tulathromycin against Chemically and Thermally Induced Pain in Mice. Pharmaceutics 2021, 13, 1247 .

AMA Style

Mohamed Elbadawy, Amira Abugomaa, Hussein M. El-Husseiny, Ahmed S. Mandour, Mohamed M. Abdel-Daim, Salama Mostafa Aboelenin, Mohamed Mohamed Soliman, Amany El-Mleeh. The Anti-Nociceptive Potential of Tulathromycin against Chemically and Thermally Induced Pain in Mice. Pharmaceutics. 2021; 13 (8):1247.

Chicago/Turabian Style

Mohamed Elbadawy; Amira Abugomaa; Hussein M. El-Husseiny; Ahmed S. Mandour; Mohamed M. Abdel-Daim; Salama Mostafa Aboelenin; Mohamed Mohamed Soliman; Amany El-Mleeh. 2021. "The Anti-Nociceptive Potential of Tulathromycin against Chemically and Thermally Induced Pain in Mice." Pharmaceutics 13, no. 8: 1247.

Journal article
Published: 03 August 2021 in Molecules
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A new preservation approach is presented in this article to prolong the lifetime of raw chicken meat and enhance its quality at 4 °C via coating with highly soluble kidney bean protein hydrolysate. The hydrolysates of the black, red, and white kidney protein (BKH, RKH, and WKH) were obtained after 30 min enzymatic hydrolysis with Alcalase (E/S ratio of 1:100, hydrolysis degree 25–29%). The different phaseolin subunits (8S) appeared in SDS-PAGE in 35–45 kD molecular weight range while vicilin appeared in the molecular weight range of 55–75 kD. The kidney bean protein hydrolysates have considerable antioxidant activity as evidenced by the DPPH-scavenging activity and β-carotine-linolenic assay, as well as antimicrobial activity evaluated by disc diffusion assay. BKH followed by RKH (800 µg/mL) significantly (p ≤ 0.05) scavenged 95, 91% of DPPH and inhibited 82–88% of linoleic oxidation. The three studied hydrolysates significantly inhibited the growth of bacteria, yeast, and fungi, where BKH was the most performing. Kidney bean protein hydrolysates could shield the chicken meat because of their amphoteric nature and many functional properties (water and oil-absorbing capacity and foaming stability). The quality of chicken meat was assessed by tracing the fluctuations in the chemical parameters (pH, met-myoglobin, lipid oxidation, and TVBN), bacterial load (total bacterial count, and psychrophilic count), color parameters and sensorial traits during cold preservation (4 °C). The hydrolysates (800 µg/g) significantly p ≤ 0.05 reduced the increment in meat pH and TVBN values, inhibited 59–70% of lipid oxidation as compared to control during 30 days of cold storage via eliminating 50% of bacterial load and maintained secured storage for 30 days. RKH and WKH significantly (p ≤ 0.05) enhanced L*, a* values, thus augmented the meat whiteness and redness, while, BKH increased b* values, declining all color parameters during meat storage. RKH and WKH (800 µg/g) (p ≤ 0.05) maintained 50–71% and 69–75% of meat color and odor, respectively, increased the meat juiciness after 30 days of cold storage. BKH, RKH and WKH can be safely incorporated into novel foods.

ACS Style

Ahmed Saad; Mahmoud Sitohy; Alshaymaa Ahmed; Nourhan Rabie; Shimaa Amin; Salama Aboelenin; Mohamed Soliman; Mohamed El-Saadony. Biochemical and Functional Characterization of Kidney Bean Protein Alcalase-Hydrolysates and Their Preservative Action on Stored Chicken Meat. Molecules 2021, 26, 4690 .

AMA Style

Ahmed Saad, Mahmoud Sitohy, Alshaymaa Ahmed, Nourhan Rabie, Shimaa Amin, Salama Aboelenin, Mohamed Soliman, Mohamed El-Saadony. Biochemical and Functional Characterization of Kidney Bean Protein Alcalase-Hydrolysates and Their Preservative Action on Stored Chicken Meat. Molecules. 2021; 26 (15):4690.

Chicago/Turabian Style

Ahmed Saad; Mahmoud Sitohy; Alshaymaa Ahmed; Nourhan Rabie; Shimaa Amin; Salama Aboelenin; Mohamed Soliman; Mohamed El-Saadony. 2021. "Biochemical and Functional Characterization of Kidney Bean Protein Alcalase-Hydrolysates and Their Preservative Action on Stored Chicken Meat." Molecules 26, no. 15: 4690.

Journal article
Published: 20 July 2021 in Veterinary Research Communications
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The aim of this study was to assess the impact of using rapeseed meal as a partial replacement for fish meal in the diet of farmed tilapia. We evaluated the effect of this replacement on growth performance, profitability, serum biomarkers, antioxidant status, gut morphology, and water quality. A total of 960 apparently healthy Oreochromis niloticus (O. niloticus) and Sarotherodon galilaeus (S. galilaeus) fingerlings were randomly distributed into four dietary treatment groups for each tilapia species (triplicate design, 120 fish/group, and 40 fish/replicate). The diets consumed by these groups were formulated to replace fish meal (FM) with rapeseed meal (RM) at 0%, 10%, 20%, and 30%, respectively, for 12 consecutive weeks. Results indicated that replacing RM in the diet of S. galilaeus (up to 20%) and O. niloticus (up to 10%) resulted in increased growth performance parameters, including final weight, weight gain, length, length gain, weight gain rate, and specific growth rate (SGR), and return parameters such as a total return and relative return compared to the control group. Moreover, an increase in RM up to 30% improved net profit and increased the mucosal length, intestinal villi length, and the number of goblet cells compared with results in its relative control groups. Additionally, we observed a significant increase in serum and liver AST and ALT with increased RM replacement. With respect to water parameters, we observed a significant difference in the ammonia levels, turbidity, and conductivity with the changes to the percentage of RM in the diets. As for the effect on each species, O. niloticus showed a more significant increase in all examined parameters compared to results in S. galilaeus. In summary, up to 10% RM can be used to replace FM without any adverse effects on the growth performance, profitability measures, intestinal morphometric analysis, or water quality.

ACS Style

Eman A Sallam; Aya F Matter; Liza S Mohammed; Aya E Azam; Ahmed Shehab; Mohamed Mohamed Soliman. Replacing fish meal with rapeseed meal: potential impact on the growth performance, profitability measures, serum biomarkers, antioxidant status, intestinal morphometric analysis, and water quality of Oreochromis niloticus and Sarotherodon galilaeus fingerlings. Veterinary Research Communications 2021, 1 .

AMA Style

Eman A Sallam, Aya F Matter, Liza S Mohammed, Aya E Azam, Ahmed Shehab, Mohamed Mohamed Soliman. Replacing fish meal with rapeseed meal: potential impact on the growth performance, profitability measures, serum biomarkers, antioxidant status, intestinal morphometric analysis, and water quality of Oreochromis niloticus and Sarotherodon galilaeus fingerlings. Veterinary Research Communications. 2021; ():1.

Chicago/Turabian Style

Eman A Sallam; Aya F Matter; Liza S Mohammed; Aya E Azam; Ahmed Shehab; Mohamed Mohamed Soliman. 2021. "Replacing fish meal with rapeseed meal: potential impact on the growth performance, profitability measures, serum biomarkers, antioxidant status, intestinal morphometric analysis, and water quality of Oreochromis niloticus and Sarotherodon galilaeus fingerlings." Veterinary Research Communications , no. : 1.

Journal article
Published: 10 June 2021 in Animals
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Dietary egg lysozyme has beneficial roles in the growth performance and health conditions of animals. The study was performed using 90 multicolored rabbits in three groups (each replicate with thirty rabbits). In the control group, rabbits were fed a diet without zinc bacitracin (ZnB) or egg lysozyme, while the second and third groups were treated with ZnB and lysozyme additive at 100 mg/kg, respectively. After eight weeks, the final weight and body weight gain (BWG) of rabbits fed dietary egg lysozyme and ZnB additives were meaningfully increased (p< 0.05). Nevertheless, the feed conversion ratio (FCR) was markedly decreased by dietary egg lysozyme and ZnB (p< 0.05). Interestingly, dietary egg lysozyme resulted in higher final weight and BWG and lower FCR than rabbits treated with ZnB (p< 0.05). Rabbits treated with egg lysozyme and ZnB additives had markedly lower populations of Clostridium spp. and Escherichia coli (p< 0.05) compared with the control. However, the counts of Lactobacillus and total bacteria were meaningfully increased in the the intestines of rabbits treated with egg lysozyme and ZnB (p< 0.05). The blood total protein and globulin of rabbits fed dietary egg lysozyme and ZnB additives were meaningfully increased (p< 0.05). Blood creatinine was significantly lowered by dietary egg lysozyme compared with the control and ZnB-treated rabbits (p< 0.05). The levels of blood urea, ALT, and AST were markedly lowered (p< 0.05) by dietary egg lysozyme and ZnB. The gene expressions of superoxide dismutase 1 (SOD1) and glutathione peroxidase (GPX) in the liver of rabbits fed dietary egg lysozyme and ZnB additives were markedly upregulated (p< 0.05) compared with the control. Dietary egg lysozyme resulted in higher expression of SOD1 and GPX genes than rabbits treated with ZnB (p< 0.05). In conclusion, the inclusion of egg lysozyme could replace the inclusion of ZnB in the diets of rabbits.

ACS Style

Mahmoud El-Deep; Khairy Amber; Yahia Eid; Salama Aboelenin; Mohamed Soliman; Mohamed Sakr; Mahmoud Dawood. The Influence of Chicken Egg Lysozyme or Zinc-Bacitracin Antibiotic on the Growth Performance, Antibacterial Capacity, Blood Profiles, and Antioxidative Status of Rabbits: A Comparative Study. Animals 2021, 11, 1731 .

AMA Style

Mahmoud El-Deep, Khairy Amber, Yahia Eid, Salama Aboelenin, Mohamed Soliman, Mohamed Sakr, Mahmoud Dawood. The Influence of Chicken Egg Lysozyme or Zinc-Bacitracin Antibiotic on the Growth Performance, Antibacterial Capacity, Blood Profiles, and Antioxidative Status of Rabbits: A Comparative Study. Animals. 2021; 11 (6):1731.

Chicago/Turabian Style

Mahmoud El-Deep; Khairy Amber; Yahia Eid; Salama Aboelenin; Mohamed Soliman; Mohamed Sakr; Mahmoud Dawood. 2021. "The Influence of Chicken Egg Lysozyme or Zinc-Bacitracin Antibiotic on the Growth Performance, Antibacterial Capacity, Blood Profiles, and Antioxidative Status of Rabbits: A Comparative Study." Animals 11, no. 6: 1731.

Journal article
Published: 05 June 2021 in Animals
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The effect of dietary orange peel (OPE) and tomato pomace extract (TPE) supplementations on growth performance, plasma biochemicals, carcass characteristics and antioxidant status of growing male rabbits were investigated. A total of 96 rabbits (5 weeks old) were distributed into four groups. The first group received untreated pelleted diet (control). The second group was fed a diet containing ascorbic acid (AA; 1.0 g/kg diet), while the third and fourth groups consumed diets supplemented with 200 gm of OPE or (TPE, respectively. Our results indicated that OPE and TPE contained 59, 14.03 mg ascorbic acid/100 g DM, respectively. Growth performance, except feed conversion ratio, and carcass weight were improved by dietary supplementations. Dietary supplementations decreased kidneys, abdominal, back fats and ether extract of meat. Plasma protein and globulin levels were high in rabbits fed AA and TPE-supplemented diets. Low plasma total cholesterol and LDL-cholesterol concentrations were observed in rabbits fed the supplemented diets. Plasma AA was increased in rabbits fed AA and OPE-supplemented diets. Rabbits fed OPE and TPE-supplemented diets had great SOD activity. The best economic efficiency was recorded by rabbits fed the supplemented diets. Dietary supplementations of OPE and TPE could effectively improve growth performance, antioxidative status, modulate AA level in plasma and meat and lower plasma total cholesterol and LDL.

ACS Style

Fawzia Amer; Nabila Elkassas; Ibrahim Salim; Shawky El-Medany; Salama Mostafa Aboelenin; Mustafa Shukry; Ayman E. Taha; Soliman Peris; Mohamed Soliman; Khalid Mahrose. Impacts of Dietary Supplementations of Orange Peel and Tomato Pomace Extracts as Natural Sources for Ascorbic Acid on Growth Performance, Carcass Characteristics, Plasma Biochemicals and Antioxidant Status of Growing Rabbits. Animals 2021, 11, 1688 .

AMA Style

Fawzia Amer, Nabila Elkassas, Ibrahim Salim, Shawky El-Medany, Salama Mostafa Aboelenin, Mustafa Shukry, Ayman E. Taha, Soliman Peris, Mohamed Soliman, Khalid Mahrose. Impacts of Dietary Supplementations of Orange Peel and Tomato Pomace Extracts as Natural Sources for Ascorbic Acid on Growth Performance, Carcass Characteristics, Plasma Biochemicals and Antioxidant Status of Growing Rabbits. Animals. 2021; 11 (6):1688.

Chicago/Turabian Style

Fawzia Amer; Nabila Elkassas; Ibrahim Salim; Shawky El-Medany; Salama Mostafa Aboelenin; Mustafa Shukry; Ayman E. Taha; Soliman Peris; Mohamed Soliman; Khalid Mahrose. 2021. "Impacts of Dietary Supplementations of Orange Peel and Tomato Pomace Extracts as Natural Sources for Ascorbic Acid on Growth Performance, Carcass Characteristics, Plasma Biochemicals and Antioxidant Status of Growing Rabbits." Animals 11, no. 6: 1688.

Journal article
Published: 31 May 2021 in Animals
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Nile tilapia Juveniles (19.50 ± 0.5 g) were fed on a basal diet (control group (CTR)) and a diet supplemented with 1 g Aspergillus oryzae (ASP) per kg diet for 12 weeks. Fish were then subjected to different salinity levels (0, 10, 15, and 20 practical salinity units (psu)) for another 15 days. Two-way ANOVA analysis revealed that the individual effects of ASP in Nile tilapia exposed to salinity levels presented a significant decrease (p< 0.05) in values of haemato-biochemical indices (such as glucose, cortisol, alanine transaminase, aspartate transaminase, and malondialdehyde) compared to those in the CTR group exposed to the same salinity levels. Moreover, significant increases (p< 0.05) of blood protein profile (albumin, globulin, and total protein), non-specific immune responses (lysozyme activity, phagocytic activity, and phagocytic index), and antioxidant enzymes activities (glutathione peroxidase, catalase, and superoxide dismutase) were observed in ASP-supplemented groups. Interestingly, there was significant (p< 0.05) downregulation of the mRNA expression values of heat shock protein 70 and interferon-gamma genes, alongside upregulation of the mRNA expression values of interleukin 1 beta and interleukin 8 genes, in the hepatic tissues of Nile tilapia in ASP-supplemented groups exposed to different salinities compared to those in the CTR group exposed to the same salinity levels. Taken together, these findings supported the potential efficacy of dietary supplementation with ASP in alleviating salinity stress-induced haemato-biochemical alterations, immune suppression, and oxidative stress in the exposed Nile tilapia.

ACS Style

Mustafa Shukry; Marwa Abd El-Kader; Basma Hendam; Mahmoud Dawood; Foad Farrag; Salama Aboelenin; Mohamed Soliman; Hany Abdel-Latif. Dietary Aspergillus oryzae Modulates Serum Biochemical Indices, Immune Responses, Oxidative Stress, and Transcription of HSP70 and Cytokine Genes in Nile Tilapia Exposed to Salinity Stress. Animals 2021, 11, 1621 .

AMA Style

Mustafa Shukry, Marwa Abd El-Kader, Basma Hendam, Mahmoud Dawood, Foad Farrag, Salama Aboelenin, Mohamed Soliman, Hany Abdel-Latif. Dietary Aspergillus oryzae Modulates Serum Biochemical Indices, Immune Responses, Oxidative Stress, and Transcription of HSP70 and Cytokine Genes in Nile Tilapia Exposed to Salinity Stress. Animals. 2021; 11 (6):1621.

Chicago/Turabian Style

Mustafa Shukry; Marwa Abd El-Kader; Basma Hendam; Mahmoud Dawood; Foad Farrag; Salama Aboelenin; Mohamed Soliman; Hany Abdel-Latif. 2021. "Dietary Aspergillus oryzae Modulates Serum Biochemical Indices, Immune Responses, Oxidative Stress, and Transcription of HSP70 and Cytokine Genes in Nile Tilapia Exposed to Salinity Stress." Animals 11, no. 6: 1621.

Journal article
Published: 24 May 2021 in Saudi Journal of Biological Sciences
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Avian influenza (AI) is a respiratory disease complex syndrome recently recorded in vaccinated flocks causing high economic losses. This study aimed to prepare inactivated vaccine from recently isolated field strains [highly pathogenic avian influenza (HPAI) (H5N8) and low pathogenic avian influenza (LPAI) (H9N2)] and compare the efficiency of the two experimental avian influenza vaccines and some commercial avian influenza H5 and H9N2 vaccines in laying hens. The obtained results indicated that the identified experimental vaccines (H5N8 and H9N2) were protected the flocks from AI as compared to commercial H5N1, H5N3, and H9N2 vaccines, which showed a protection level of 80, 70, and 90%, respectively, indicating a high efficacy for the developed vaccines. In addition, it significantly improved the virus shedding, especially when used in booster dose. The experimental vaccines were given high antibody titer higher than commercial vaccine which was reached to 9.3 log2, 9.7log2 for experimental H5N8 vaccine which was significantly higher than and groups 3 and 4 especially at 2nd WPV, while at the 3rd WPV, the significant difference was with group 4 only. The HI titer was 9.3 log2 at 2nd WPV for the experimental H9N2 vaccine that was significantly higher than group 9. In conclusion, the booster dose of the experimental vaccines could elicit strong immunity than single-dose and commercial vaccines.

ACS Style

Ahmed M.E. Hegazy; Nahed Yehia; Abeer F.I. Hassan; Mohamed. T. El-Saadony; Salama Mostafa Aboelenin; Mohamed M. Soliman; Hala M.N. Tolba. The potency of newly development H5N8 and H9N2 avian influenza vaccines against the isolated strains in laying hens from Egypt during 2019. Saudi Journal of Biological Sciences 2021, 28, 5310 -5316.

AMA Style

Ahmed M.E. Hegazy, Nahed Yehia, Abeer F.I. Hassan, Mohamed. T. El-Saadony, Salama Mostafa Aboelenin, Mohamed M. Soliman, Hala M.N. Tolba. The potency of newly development H5N8 and H9N2 avian influenza vaccines against the isolated strains in laying hens from Egypt during 2019. Saudi Journal of Biological Sciences. 2021; 28 (9):5310-5316.

Chicago/Turabian Style

Ahmed M.E. Hegazy; Nahed Yehia; Abeer F.I. Hassan; Mohamed. T. El-Saadony; Salama Mostafa Aboelenin; Mohamed M. Soliman; Hala M.N. Tolba. 2021. "The potency of newly development H5N8 and H9N2 avian influenza vaccines against the isolated strains in laying hens from Egypt during 2019." Saudi Journal of Biological Sciences 28, no. 9: 5310-5316.

Original research
Published: 22 May 2021 in Food Science & Nutrition
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Gentamicin is an effective antibiotic that has been used worldwide for many years. While considered an essential medicine by the WHO, gentamicin can also lead to severe kidney damage. This study explored the ameliorative effects of Glycyrrhiza glabra root extract on gentamicin-induced renal injury in mice. Four groups of n = 7 mice were used: (a) control; (b) G. glabra-only; (c) gentamicin-only; and (d) gentamicin plus G. glabra. Kidney samples were tested for: antioxidant enzyme activity (superoxide dismutase [SOD] and glutathione peroxidase [Gpx]); expression of HO-1 and nuclear factor erythroid 2-related factor 2 genes; expression of Cox-2 and Bax; cytokine levels (IL-1β, and IL-6); histopathological anomalies; and standard renal functional component levels (creatinine, urea, and blood urea nitrogen). The effects of gentamicin were generally reversed or normalized following treatment with G. glabra root extract. Gentamicin decreased Gpx and SOD parameters and increased IL-1 β and IL-6 levels, but these returned to normal in the G. glabra-treated group. Gentamicin upregulated tissue levels of Cox-2 and Bax, and downregulated HO-1 and Nrf-2 expression but again, and these levels returned to normal in the group treated with G. glabra. Mice that had received gentamicin exhibited acute renal blood vessel congestion, focal interstitial round cell aggregation, and hydropic degeneration of renal tubular epithelium. However, those that had also received G. glabra showed a normal histopathology. Findings from this study indicate that in mouse models, gentamicin-induced kidney damage can be reversed or ameliorated by administering G. glabra, so it can be considered as an effective complimentary therapy.

ACS Style

Mohamed A. Nassan; Mohamed M. Soliman; Adil Aldhahrani; Fayez Althobaiti; Adel Q. Alkhedaide. Ameliorative impacts of Glycyrrhiza glabra root extract against nephrotoxicity induced by gentamicin in mice. Food Science & Nutrition 2021, 9, 3405 -3413.

AMA Style

Mohamed A. Nassan, Mohamed M. Soliman, Adil Aldhahrani, Fayez Althobaiti, Adel Q. Alkhedaide. Ameliorative impacts of Glycyrrhiza glabra root extract against nephrotoxicity induced by gentamicin in mice. Food Science & Nutrition. 2021; 9 (7):3405-3413.

Chicago/Turabian Style

Mohamed A. Nassan; Mohamed M. Soliman; Adil Aldhahrani; Fayez Althobaiti; Adel Q. Alkhedaide. 2021. "Ameliorative impacts of Glycyrrhiza glabra root extract against nephrotoxicity induced by gentamicin in mice." Food Science & Nutrition 9, no. 7: 3405-3413.

Journal article
Published: 21 May 2021 in Antioxidants
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This study examined the effect of sodium salicylates (SS), alone and in combination with curcumin (CUR), on kidney function and architecture in rats. Five rat groups were given 1 mL physiological saline/rat orally, 1 mL olive oil/rat orally, 50 mg CUR/kg bwt orally, 300 mg SS/kg bwt intraperitoneally, or CUR+SS for 15 days. The hematological indices, serum protein profile, serum electrolytes balance, oxidative stress, and lipid peroxidation of kidney tissues were assessed. The histopathological examination and immune expression of Caspase-3 and nuclear factor kappa (NF-κB) were conducted. The findings showed that SS injection induced nephrotoxic activity, including increased serum urea, creatinine, and uric acid levels. It also caused apparent pathological alterations with increased Caspase-3 and NF-κB immuno-expression. In addition, thrombocytopenia, leukocytosis, neutrophilia, hyponatremia, hypochloremia, hypocalcemia, and hypomagnesemia but not hyperkalemia and hyperphosphatemia were evident in SS-injected rats. Moreover, SS exposure increased serum α1 globulin, renal tissue malondialdehyde, and Caspase-3 levels but superoxide dismutase, glutathione peroxidase, and Bcl-2 levels declined. Meanwhile, CUR significantly counteracted the SS harmful impacts on kidneys but SS+CUR co-administration induced an anemic condition. Overall, CUR has an evident protective role against SS-induced renal damage, but the disturbed hematological alterations should be carefully taken into consideration in their combined use.

ACS Style

Yasmina Abd-Elhakim; Attia Moselhy; Adil Aldhahrani; Rasha Beheiry; Wafaa Mohamed; Mohamed Soliman; Bayan Saffaf; Maha M. El Deib. Protective Effect of Curcumin against Sodium Salicylate-Induced Oxidative Kidney Damage, Nuclear Factor-Kappa Dysregulation, and Apoptotic Consequences in Rats. Antioxidants 2021, 10, 826 .

AMA Style

Yasmina Abd-Elhakim, Attia Moselhy, Adil Aldhahrani, Rasha Beheiry, Wafaa Mohamed, Mohamed Soliman, Bayan Saffaf, Maha M. El Deib. Protective Effect of Curcumin against Sodium Salicylate-Induced Oxidative Kidney Damage, Nuclear Factor-Kappa Dysregulation, and Apoptotic Consequences in Rats. Antioxidants. 2021; 10 (6):826.

Chicago/Turabian Style

Yasmina Abd-Elhakim; Attia Moselhy; Adil Aldhahrani; Rasha Beheiry; Wafaa Mohamed; Mohamed Soliman; Bayan Saffaf; Maha M. El Deib. 2021. "Protective Effect of Curcumin against Sodium Salicylate-Induced Oxidative Kidney Damage, Nuclear Factor-Kappa Dysregulation, and Apoptotic Consequences in Rats." Antioxidants 10, no. 6: 826.

Journal article
Published: 11 May 2021 in Animals
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The existing study was designed to assess the influences of dietary activated charcoal (AC) on the growth performance, immune responses, antioxidative status, and its mitigating roles against the physiological responses of Nile tilapia exposed a sub-lethal dose of a neonicotinoid agriculture pesticide, namely, as imidacloprid (IMID). Nile tilapia juveniles were fed on diets supplemented with graded AC levels as 0 (control), 5, 10, 15, and 20 g/kg diet for eight weeks. Growth, hemato-biochemical indices, and antioxidant and immune responses of fish in all groups were evaluated at the end of the feeding experiment. Afterward, fish in all experimental groups were subjected to a sub-lethal dose of IMID (0.0109 μg/L) for two weeks. Then, fish mortalities, stress indicators, and IMID residual levels in liver and flesh were examined. Results of the feeding experiment showed that total feed intake, weight gain, final body weights, and feed efficiency ratio were significantly increased in all AC groups compared with the control group. The survival rate was 100% in all experimental groups. No statistical differences were observed in the hematological picture of all experimental groups except the lymphocyte count, which was significantly increased in all AC groups compared to the control group. Total protein, albumin, globulin, nitric oxide levels, lysozyme, and respiratory burst activities were significantly increased in all AC groups. Serum alanine transaminase, aspartate transaminase, alkaline phosphatase activities, and malondialdehyde (MDA) levels were significantly decreased in all AC groups compared with the AC0 group. After exposure to a sub-lethal dose of IMID, survival rates were significantly elevated, and IMID residual levels in liver and flesh were significantly decreased in all AC groups than in the control group. Moreover, second-order polynomial regression showed that dietary supplementation with 14.30 g AC/kg diet resulted in the lowest blood glucose and serum MDA levels. Conclusively, we suggest dietary supplementation with 14.30 g AC/kg diet to modulate physiological responses of Nile tilapia to sub-lethal IMID toxicity.

ACS Style

Samah Abd El-Hameed; Samar Negm; Nahla Ismael; Mohammed Naiel; Mohamed Soliman; Mustafa Shukry; Hany Abdel-Latif. Effects of Activated Charcoal on Growth, Immunity, Oxidative Stress Markers, and Physiological Responses of Nile Tilapia Exposed to Sub-Lethal Imidacloprid Toxicity. Animals 2021, 11, 1357 .

AMA Style

Samah Abd El-Hameed, Samar Negm, Nahla Ismael, Mohammed Naiel, Mohamed Soliman, Mustafa Shukry, Hany Abdel-Latif. Effects of Activated Charcoal on Growth, Immunity, Oxidative Stress Markers, and Physiological Responses of Nile Tilapia Exposed to Sub-Lethal Imidacloprid Toxicity. Animals. 2021; 11 (5):1357.

Chicago/Turabian Style

Samah Abd El-Hameed; Samar Negm; Nahla Ismael; Mohammed Naiel; Mohamed Soliman; Mustafa Shukry; Hany Abdel-Latif. 2021. "Effects of Activated Charcoal on Growth, Immunity, Oxidative Stress Markers, and Physiological Responses of Nile Tilapia Exposed to Sub-Lethal Imidacloprid Toxicity." Animals 11, no. 5: 1357.

Journal article
Published: 19 April 2021 in Animals
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The objective of this study was to examine the effects of diets supplemented with corn silk meal (CSM) and non-starch polysaccharide (NSP) enzyme on growth performance, blood biochemistry, immunological response, and growth-related gene expression in broiler chickens. A total of 270 broiler chickens were divided into six experimental groups: (1) basal diets (BD) as control; (2) BD supplemented with 0.5 g/kg feed NSP enzyme; the other four groups are CSM diets as following; (3) and (4) fed diet contain 40 and 80 kg/ton of CSM; (5) and (6) fed diet contain 40 and 80 kg/ton CSM and supplemented with 0.5 g/kg NSP enzyme. Body weight gain (BWG), feed conversion ratio (FCR), protein retention and fiber digestibility were synergistically improved (p < 0.05) when fed CSM supplemented with NSP enzyme. Moreover, a synergistic decrease (p < 0.05) in the serum glucose and total cholesterol were found. Immune organ weights and Newcastle disease virus titers were increased with CSM diets. Interestingly, the relative mRNAs of the growth hormone receptor (GHR) and insulin growth factor (IGF) were increased (p < 0.05) with the CSM and NSP enzyme: the relative mRNA expressions of cholecystokinin (CCK) and leptin were decreased by feeding CSM diets with the NSP enzyme. It could be concluded that the dietary inclusion of CSM with the NSP enzyme might improve growth performance, modify plasma lipids, and enhance immune response in broilers.

ACS Style

Abeer Kirrella; Safaa Abdo; Karima El-Naggar; Mohamed Soliman; Salama Aboelenin; Mahmoud Dawood; Ahmed Saleh. Use of Corn Silk Meal in Broiler Diet: Effect on Growth Performance, Blood Biochemistry, Immunological Responses, and Growth-Related Gene Expression. Animals 2021, 11, 1170 .

AMA Style

Abeer Kirrella, Safaa Abdo, Karima El-Naggar, Mohamed Soliman, Salama Aboelenin, Mahmoud Dawood, Ahmed Saleh. Use of Corn Silk Meal in Broiler Diet: Effect on Growth Performance, Blood Biochemistry, Immunological Responses, and Growth-Related Gene Expression. Animals. 2021; 11 (4):1170.

Chicago/Turabian Style

Abeer Kirrella; Safaa Abdo; Karima El-Naggar; Mohamed Soliman; Salama Aboelenin; Mahmoud Dawood; Ahmed Saleh. 2021. "Use of Corn Silk Meal in Broiler Diet: Effect on Growth Performance, Blood Biochemistry, Immunological Responses, and Growth-Related Gene Expression." Animals 11, no. 4: 1170.

Journal article
Published: 13 April 2021
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Mabrouk Attia Abd Eldaim; Ehab Tousson; Mohamed Mohamed Soliman; Ibrahim El Tantawy El Sayed; Abdel Aleem H Abdel Aleem; Hebatalla Nashaat Elsharkawy. Grape seed extract ameliorated Ehrlich solid tumor-induced hepatic tissue and DNA damage with reduction of PCNA and P53 protein expression in mice. 2021, 1 .

AMA Style

Mabrouk Attia Abd Eldaim, Ehab Tousson, Mohamed Mohamed Soliman, Ibrahim El Tantawy El Sayed, Abdel Aleem H Abdel Aleem, Hebatalla Nashaat Elsharkawy. Grape seed extract ameliorated Ehrlich solid tumor-induced hepatic tissue and DNA damage with reduction of PCNA and P53 protein expression in mice. . 2021; ():1.

Chicago/Turabian Style

Mabrouk Attia Abd Eldaim; Ehab Tousson; Mohamed Mohamed Soliman; Ibrahim El Tantawy El Sayed; Abdel Aleem H Abdel Aleem; Hebatalla Nashaat Elsharkawy. 2021. "Grape seed extract ameliorated Ehrlich solid tumor-induced hepatic tissue and DNA damage with reduction of PCNA and P53 protein expression in mice." , no. : 1.

Research article
Published: 13 April 2021 in Environmental Science and Pollution Research
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This study evaluated the ameliorative potential of grape seed extract (GSE) against Ehrlich solid tumor (EST)–induced hepatic tissue alterations in mice. The control group was infused with physiological saline. The second group received GSE (50 mg/kg day by day orally) for 2 weeks. The third group was subcutaneously injected with 2.5 million of EST cells. The fourth group was injected with EST cells and treated with GSE extract simultaneously. The fifth group was injected with EST cells and kept for 2 weeks until the appearance of a solid tumor, then treated with GSE for 2 weeks. The phytochemical analysis of GSE revealed the presence of total phenols (17.442 mg GAE/g) and total flavonoid (6.687 mg CE/g) with antioxidant activity of 81.506 mg TE/g DPPH. The Ehrlich solid tumor significantly raised the activities of ALT, AST, and ALP; the level of alpha fetoprotein (AFP) in serum; and the protein expressions of hepatic proliferating cell nuclear antigen (PCNA) and tumor suppressor protein (P53), as well as induced DNA damage and pathological alterations in liver tissue. However, it significantly reduced serum albumin and total protein levels. In contrast, the co- or post-treatment of EST-bearing mice with GSE reduced the activities of ALT, AST, and ALP; the level AFP in serum; and hepatic P53 and PCNA protein expressions. In addition, it reduced EST-induced hepatic DNA damage and pathological alterations, while it increased serum albumin and total protein levels. This study suggested that GSE is a potent hepatoprotective agent and both co- and post-treatment of EST-bearing mice with GSE almost had the same effects.

ACS Style

Mabrouk Attia Abd Eldaim; Ehab Tousson; Mohamed Mohamed Soliman; Ibrahim El Tantawy El Sayed; Abdel Aleem H. Abdel Aleem; Hebatalla Nashaat Elsharkawy. Grape seed extract ameliorated Ehrlich solid tumor-induced hepatic tissue and DNA damage with reduction of PCNA and P53 protein expression in mice. Environmental Science and Pollution Research 2021, 28, 44226 -44238.

AMA Style

Mabrouk Attia Abd Eldaim, Ehab Tousson, Mohamed Mohamed Soliman, Ibrahim El Tantawy El Sayed, Abdel Aleem H. Abdel Aleem, Hebatalla Nashaat Elsharkawy. Grape seed extract ameliorated Ehrlich solid tumor-induced hepatic tissue and DNA damage with reduction of PCNA and P53 protein expression in mice. Environmental Science and Pollution Research. 2021; 28 (32):44226-44238.

Chicago/Turabian Style

Mabrouk Attia Abd Eldaim; Ehab Tousson; Mohamed Mohamed Soliman; Ibrahim El Tantawy El Sayed; Abdel Aleem H. Abdel Aleem; Hebatalla Nashaat Elsharkawy. 2021. "Grape seed extract ameliorated Ehrlich solid tumor-induced hepatic tissue and DNA damage with reduction of PCNA and P53 protein expression in mice." Environmental Science and Pollution Research 28, no. 32: 44226-44238.

Full article
Published: 26 March 2021 in Journal of Food Biochemistry
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Thyme (Thymus vulgaris) is an herbal plant with pleiotropic medicinal properties. In this study, we examined the possible protective effect of an ethanolic extract of thyme leaves against the renal oxidative stress induced by sodium nitrite (NaNO2). Male Swiss mice received either saline or thyme extract for 15 days (0.5 g/kg body weight, orally). NaNO2 (60 mg/kg) was injected intraperitoneally at Day 14. The protective group received the thyme extract for 15 days and NaNO2 on Day 14. Blood and kidney samples were taken from all groups to measure serum urea, blood urea nitrogen (BUN), creatinine, serum, tissue antioxidant activity, and the inflammatory cytokines IL‐1β and IL‐6. Quantitative real‐time PCR (qRT‐PCR) was used to examine the expression of kidney injury marker‐1 (Kim‐1), TNF‐α, nuclear factor erythroid‐2 related factor 2 (Nrf2), and hemoxygenase‐1 (HO‐1), all of which are associated with kidney redox and oxidative stress. Pretreatment with thyme extract reduced the effects of NaNO2 on urea, BUN, and creatinine, and reversed its effect on tissue and serum antioxidants. NaNO2‐induced nephritis as demonstrated by the upregulation in mRNA expression of Kim‐1 and TNF‐α, which was, however, recovered and protected by pretreatment with thyme extract. Expression of Nrf2 and HO‐1 was upregulated by treatment with thyme extract and downregulated by NaNO2 intoxication. NaNO2‐induced congestion in glomeruli and dilatation of the renal tubules, conditions that were restored in the group pretreated with thyme extract. NaNO2 upregulated Bax immunoreactivity and caused apoptosis in renal structures. Thus, thyme extract is effective in managing the renal toxicity associated with oxidative stress and renal redox. Practical applications The results from this study have shown that use of thyme extract may promote better health due to its high antioxidant activity. For instance, it could be ingested to alleviate the symptoms of renal inflammation and oxidative stress associated with nitrite toxicity. Thyme extract regulated renal redox, oxidative stress, antioxidant levels, and inflammation‐associated genes at the molecular, biochemical, and cellular immunohistochemical levels.

ACS Style

Mohamed Mohamed Soliman; Adel Aldhahrani; Yousef Saeed Alghamdi; Alshaimaa Mohammed Said. Impact of Thymus vulgaris extract on sodium nitrite‐induced alteration of renal redox and oxidative stress: Biochemical, molecular, and immunohistochemical study. Journal of Food Biochemistry 2021, e13630 .

AMA Style

Mohamed Mohamed Soliman, Adel Aldhahrani, Yousef Saeed Alghamdi, Alshaimaa Mohammed Said. Impact of Thymus vulgaris extract on sodium nitrite‐induced alteration of renal redox and oxidative stress: Biochemical, molecular, and immunohistochemical study. Journal of Food Biochemistry. 2021; ():e13630.

Chicago/Turabian Style

Mohamed Mohamed Soliman; Adel Aldhahrani; Yousef Saeed Alghamdi; Alshaimaa Mohammed Said. 2021. "Impact of Thymus vulgaris extract on sodium nitrite‐induced alteration of renal redox and oxidative stress: Biochemical, molecular, and immunohistochemical study." Journal of Food Biochemistry , no. : e13630.

Journal article
Published: 23 March 2021 in Sustainability
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The practical usage of untraditional feedstuffs such as sunflower meal (SFM) in laying hens nutrition in developing countries has received considerable attention. SFM is a by-product of the sunflower oil industry and has been progressively added to bird’s diets. Sunflower meal (SFM) is gaining great interest as a feed ingredient due to its eminent crude protein content, low anti-nutritional compounds, and low price. The current experiment was aimed to assess the production efficiency, egg quality, yolk fatty acids composition, and nutrient digestibility of laying hens fed SFM. A total of 162 Bovans Brown laying hens aged 60 weeks old were randomly allocated using a completely randomized design into three experimental groups of nine replicates each (n = six/replicate) for eight weeks. The dietary treatments involved a control (basal diet) and two levels of SFM, 50 and 100 g/kg feed. The dietary treatments did not influence live weight gain, feed intake, and egg mass. On one hand, the laying rate was increased; on the other hand, the feed conversion ratio and broken eggs rate of laying hens were decreased (p < 0.05) by the dietary inclusion of SFM. Dietary treatments had no effect on the egg’s quality characteristics except the yolk color and yolk height were larger (p = 0.01) for laying hens fed SFM compared with those fed the control. Dietary inclusion of SFM decreased (p < 0.05) the content of cholesterol in the egg yolk. Still, it increased the yolk contents of vitamin E, calcium, linoleic acid, linolenic acid, and oleic acid (p < 0.05). Furthermore, the dietary inclusion of SFM increased crude protein and calcium digestibility, but decreased the ether extract digestibility. In conclusion, our results suggested that the dietary inclusion of SFM, up to 100 g/kg at a late phase of laying, could improve the production performance, some of the egg quality traits, and nutrient digestibility while decreasing egg yolk cholesterol.

ACS Style

Ahmed Saleh; Ahmed El-Awady; Khairy Amber; Yahya Eid; Mohammed Alzawqari; Shaimaa Selim; Mohamed Soliman; Mustafa Shukry. Effects of Sunflower Meal Supplementation as a Complementary Protein Source in the Laying Hen’s Diet on Productive Performance, Egg Quality, and Nutrient Digestibility. Sustainability 2021, 13, 3557 .

AMA Style

Ahmed Saleh, Ahmed El-Awady, Khairy Amber, Yahya Eid, Mohammed Alzawqari, Shaimaa Selim, Mohamed Soliman, Mustafa Shukry. Effects of Sunflower Meal Supplementation as a Complementary Protein Source in the Laying Hen’s Diet on Productive Performance, Egg Quality, and Nutrient Digestibility. Sustainability. 2021; 13 (6):3557.

Chicago/Turabian Style

Ahmed Saleh; Ahmed El-Awady; Khairy Amber; Yahya Eid; Mohammed Alzawqari; Shaimaa Selim; Mohamed Soliman; Mustafa Shukry. 2021. "Effects of Sunflower Meal Supplementation as a Complementary Protein Source in the Laying Hen’s Diet on Productive Performance, Egg Quality, and Nutrient Digestibility." Sustainability 13, no. 6: 3557.

Full article
Published: 22 March 2021 in Journal of Food Biochemistry
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The extensive usage of gibberellic acid (GA3) in agriculture and plant growth is generally associated with enormous human and public health hazards. The present research assesses the impact of n‐acetyl cysteine (NAC) on the hepatorenal injury persuaded by GA3 for this purpose, After two weeks of adaptation twenty‐four rats allocated into four groups (6 rats/group) as follows: control group, supplied with saline only; n‐acetyl cysteine (NAC) group, provided with 150 mg/kg/bw by stomach tube (orally) dissolved in saline; Positive GA3 group, received GA3 (55 mg/kg/bw) orally; Protective group received NAC (150 mg/kg/bw) and GA3 (55 mg/kg/bw) as in NAC and GA3 groups. Rats received their treatments for consecutive 3 weeks. On day 22, rats were anesthetized, then euthanized. Blood and tissue samples were obtained for biochemical, antioxidants markers analysis, gene expression, and histopathological examination. Our results revealed significant changes in serum AST, ALT, urea, uric acid, total protein, and albumin levels with a substantial rise of MDA and NO concentration in GA3 treated rats along with a considerable decrease of the GSH and overexpression of the inflammatory hepatic and renal cytokines (IL‐10, TNF‐α, NOS) and fibrotic gene expression TGF‐β1, and α‐SMA, with boost expression of nuclear factor‐kappa (NFkB). NAC co‐administered with GA3 significantly normalized the kidney and liver function and the antioxidant state, besides normal histological structure of both liver and kidney tissue and downregulated expression of the pro‐inflammatory cytokines as well as, fibrogenic gene expression. Practical applications The current study confirmed that GA3 induced hepto‐renal dysfunction that was ameliorated by NAC administration. Moreover, our findings confirmed the antioxidant capability of n‐acetyl cysteine and afford robust evidence about the ameliorative effect of the n‐acetyl cysteine to attenuate the hepatorenal injury induced by gibberellic acid through modulation of the antioxidant defense system fibrogenic, and pro‐inflammatory cytokines expression.

ACS Style

Mohamed Mohamed Soliman; Adil Aldhahrani; Ahmed Gaber; Walaa F. Alsanie; Mustafa Shukry; Wafaa Abdou Mohamed; Mohamed M. M. Metwally. Impacts of n‐acetyl cysteine on gibberellic acid‐induced hepatorenal dysfunction through modulation of pro‐inflammatory cytokines, antifibrotic and antioxidant activity. Journal of Food Biochemistry 2021, 45, e13706 .

AMA Style

Mohamed Mohamed Soliman, Adil Aldhahrani, Ahmed Gaber, Walaa F. Alsanie, Mustafa Shukry, Wafaa Abdou Mohamed, Mohamed M. M. Metwally. Impacts of n‐acetyl cysteine on gibberellic acid‐induced hepatorenal dysfunction through modulation of pro‐inflammatory cytokines, antifibrotic and antioxidant activity. Journal of Food Biochemistry. 2021; 45 (4):e13706.

Chicago/Turabian Style

Mohamed Mohamed Soliman; Adil Aldhahrani; Ahmed Gaber; Walaa F. Alsanie; Mustafa Shukry; Wafaa Abdou Mohamed; Mohamed M. M. Metwally. 2021. "Impacts of n‐acetyl cysteine on gibberellic acid‐induced hepatorenal dysfunction through modulation of pro‐inflammatory cytokines, antifibrotic and antioxidant activity." Journal of Food Biochemistry 45, no. 4: e13706.

Journal article
Published: 20 March 2021 in Animals
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Doxorubicin (DOX) has a potent antineoplastic efficacy and is considered a cornerstone of chemotherapy. However, it causes several dose-dependent cardiotoxic results, which has substantially restricted its clinical application. This study was intended to explore the potential ameliorative effect of date palm pollen ethanolic extract (DPPE) against DOX-induced cardiotoxicity and the mechanisms underlying it. Forty male Wistar albino rats were equally allocated into Control (CTR), DPPE (500 mg/kg bw for 4 weeks), DOX (2.5 mg/kg bw, intraperitoneally six times over 2 weeks), and DPPE + DOX-treated groups. Pre-coadministration of DPPE with DOX partially ameliorated DOX-induced cardiotoxicity as DPPE improved DOX-induced body and heart weight changes and mitigated the elevated cardiac injury markers activities of serum aminotransferases, lactate dehydrogenase, creatine kinase, and creatine kinase-cardiac type isoenzyme. Additionally, the concentration of serum cardiac troponin I (cTnI), troponin T (cTnT), N-terminal pro-brain natriuretic peptide (NT-pro BNP), and cytosolic calcium (Ca+2) were amplified. DPPE also alleviated nitrosative status (nitric oxide) in DOX-treated animals, lipid peroxidation and antioxidant molecules as glutathione content, and glutathione peroxidase, catalase, and superoxide dismutase activities and inflammatory markers levels; NF-κB p65, TNF-α, IL-1β, and IL-6. As well, it ameliorated the severity of histopathological lesions, histomorphometric alteration and improved the immune-staining of the pro-fibrotic (TGF-β1), pro-apoptotic (caspase-3 and Bax), and anti-apoptotic (Bcl-2) proteins in cardiac tissues. Collectively, pre-coadministration of DPPE partially mitigated DOX-induced cardiac injuries via its antioxidant, anti-inflammatory, anti-fibrotic, and anti-apoptotic potential.

ACS Style

Samar Elblehi; Yasser El-Sayed; Mohamed Soliman; Mustafa Shukry. Date Palm Pollen Extract Avert Doxorubicin-Induced Cardiomyopathy Fibrosis and Associated Oxidative/Nitrosative Stress, Inflammatory Cascade, and Apoptosis-Targeting Bax/Bcl-2 and Caspase-3 Signaling Pathways. Animals 2021, 11, 886 .

AMA Style

Samar Elblehi, Yasser El-Sayed, Mohamed Soliman, Mustafa Shukry. Date Palm Pollen Extract Avert Doxorubicin-Induced Cardiomyopathy Fibrosis and Associated Oxidative/Nitrosative Stress, Inflammatory Cascade, and Apoptosis-Targeting Bax/Bcl-2 and Caspase-3 Signaling Pathways. Animals. 2021; 11 (3):886.

Chicago/Turabian Style

Samar Elblehi; Yasser El-Sayed; Mohamed Soliman; Mustafa Shukry. 2021. "Date Palm Pollen Extract Avert Doxorubicin-Induced Cardiomyopathy Fibrosis and Associated Oxidative/Nitrosative Stress, Inflammatory Cascade, and Apoptosis-Targeting Bax/Bcl-2 and Caspase-3 Signaling Pathways." Animals 11, no. 3: 886.

Original article
Published: 16 March 2021 in Andrologia
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In agriculture, gibberellic acid (GA3) is commonly used with extreme dangers for public health. The current research evaluates the improving effects of n‐acetyl cysteine (NAC, 150 mg/kg bw) co‐administered with GA3 (55 mg/kg bw) mediated testicular injury. Twenty‐four male albino rats were split into 4 groups: Negative control (CNT), NAC group, positive GA3 group and protective group, co‐administered NAC plus GA3. On day 21, rats were anesthetised then euthanised by decapitation. Blood samples were collected; testicular samples were taken for semen analysis, serum chemistry, RNA extraction, histological and antioxidants markers examination. Our results revealed a significant decline p < .05 of catalase level and total antioxidant capacity. There was a substantial rise of MDA concentration in GA3‐treated rats along with a considerable decrease of the antioxidant markers (SOD, GSH) and serum male reproductive hormones. In GA3‐treated rats, an overexpression of the inflammatory cytokines (TNF‐α, IL‐1β) and anti‐inflammatory cytokine IL‐10 with boost mRNA expression of nuclear factor‐kappa (NFkB) were confirmed. There was downregulation of steroidogenesis genes and decrease in sperm quality and concentration with an increase in sperm abnormalities, all were reported in GA3‐treated rats. NAC treatment significantly increased the antioxidant state, testicular function beside structural germ cell and seminiferous tubules histology accompanied by upsurge of steroidogenic mRNA expressions (P450scc and 3β‐HSD) and downregulated the pro‐inflammatory cytokines mRNA expression (TNF‐α, IL‐1β). These results confirm the antioxidant capability of NAC and afford robust evidence about the ameliorative effect of the NAC to attenuate the testicular injury induced by GA3 through modulation of the antioxidant defence system, steroidogenic and pro‐inflammatory cytokines mRNA expression.

ACS Style

Mohamed Mohamed Soliman; Adil Aldhahrani; Ahmed Gaber; Walaa F. Alsanie; Mustafa Shukry; Wafaa Abdou Mohamed; Mohamed M. M. Metwally; Alaa Ahmed Mohamed. Impacts of n‐acetyl cysteine on gibberellic acid‐induced testicular dysfunction through regulation of inflammatory cytokines, steroid and antioxidant activity. Andrologia 2021, 53, e14036 .

AMA Style

Mohamed Mohamed Soliman, Adil Aldhahrani, Ahmed Gaber, Walaa F. Alsanie, Mustafa Shukry, Wafaa Abdou Mohamed, Mohamed M. M. Metwally, Alaa Ahmed Mohamed. Impacts of n‐acetyl cysteine on gibberellic acid‐induced testicular dysfunction through regulation of inflammatory cytokines, steroid and antioxidant activity. Andrologia. 2021; 53 (5):e14036.

Chicago/Turabian Style

Mohamed Mohamed Soliman; Adil Aldhahrani; Ahmed Gaber; Walaa F. Alsanie; Mustafa Shukry; Wafaa Abdou Mohamed; Mohamed M. M. Metwally; Alaa Ahmed Mohamed. 2021. "Impacts of n‐acetyl cysteine on gibberellic acid‐induced testicular dysfunction through regulation of inflammatory cytokines, steroid and antioxidant activity." Andrologia 53, no. 5: e14036.