Cold metal transfer (CMT)-based wire and arc additive manufacturing (WAAM) of Ti-6Al-4V alloy has been investigated to manufacture walls with two different building strategies. This study focuses on the influence of the application of thermal treatments on the resulting microstructure and mechanical properties. Deep microstructural analysis revealed different grades of growth of lamellae α phase after several thermal treatments at different temperatures, which lead to different tensile mechanical properties and better strength and ductility balance compared to the as-built condition. Results are compared with equivalent forged and casting standards and the state of the art for WAAM of Ti-6Al-4V alloy. At temperatures of 920 °C, anisotropy was maintained and elongation increased by 70% while yield strength and UTS was slightly decreased by 8%.