Asyiah IN, Mudakir I, Hoesain M, Pradana AP, Djunaidy A, Sari RF. 2020. Consortium of endophytic bacteria and rhizobacteria effectively suppresses the population of Pratylenchus coffeae and promotes the growth of Robusta coffee. Biodiversitas 21: 4702-4708. Pratylenchus coffeae as the coffee-parasitic nematode can cause a yield loss from 28.7% to 78.4% on Robusta coffee (Coffea canephora A. Froehner). Environmentally friendly and sustainable efforts can be made to control this nematode by applying the antagonistic agents from the bacterial group. The application of antagonistic agents in a consortium has a better effectivity than a single application. This study was aimed to identify the effectivity of the consortium of endophytic bacteria and rhizobacteria formulated on a cost-effective molasse medium against the P. coffeae population and growth of Robusta coffee in a greenhouse. A total of nine bacterial isolates of genus Bacillus and Pseudomonas were used during experiment. The formulation was prepared by mixing the bacterial suspension and 2% molasses with a ratio of 1:1 (v/v). The greenhouse assay was performed following the completely randomized block design pattern with five treatments and five replications. Robusta coffee plant clone BP 936 was treated with a 10 mL (109 CFU mL-1) bacterial consortium mixture while control was treated with 10 mL sterilized water. Each plant was inoculated with P. coffeae 50 J2 after a week of bacterial consortium application. The result showed that all bacterial consortiums were effective in suppressing the population of P. coffeae in soil and root of Robusta coffee. All consortiums gave a better result than control in suppressing the population of P. coffeae. The best suppression of nematodes was shown by the Cst 02 consortium with the suppression value of 62.5% in the root and 76.1% in the coffee plant rhizosphere. The application of bacterial consortium increases the plant height (28.02-44.51%), number of leaves (127.27-168.18%), and leaf area (278.8-425.57%). In this study, suppression of P. coffeae population by bacterial consortium showed a positive correlation with plant growth. This study presents the information of endophytic bacteria and rhizobacteria consortium from Bacillus and Pseudomonas group that is effective in suppressing the population P. coffeae and improves the growth of Robusta coffee.

Bawean Island is located in the middle of the Java Sea, about 150 km north of Gresik. The name Bawean was taken from the Sanskrit which means there is sunlight. The Bawean people are acculturation from several ethnic groups of Java, Madura, Kalimantan, Sulawesi and Sumatera including their culture and language. The majority of Bawean residents work as fishermen. Bawean Island fishermen utilize marine resources to meet the needs of their family by using knowledge that has been obtained from previous generations. The purpose of this study was to determine the use of traditional fishing gear and fishing strategies used by fishing communities in Bawean Island. The research method used was direct observation, and sampling using Purposive Sampling and Snowball Sampling. The results showed that the fishing gears used by Bawean Island fishermen were kareket (waring), pajeng (payang), jhering cursor (ring net), rompon (bubu), jhering pemhir (drift gill net), payimbek rentengan (drag fishing), gerany Panyimbek (drag fishing line), panceng (fishing line). These fishing gears are very environmentally friendly because the usage does not damage the seabed ecosystem. Each fishing gear has its own characteristics, so that different fishing gear will result in different fishing catch

The damage caused by the southern root-knot nematode (Meloidogyne incognita) in coffee plants has been reported in various countries, including Indonesia. The measures to control the nematodes on coffee seedlings and fields depend on synthetic nematicides. Addressing this issue requires not only a more environmentally friendly and cheaper technology but also equal effectiveness comparable to synthetic nematicides. This study aimed to test the effectiveness of fortified bacterial consortium (FBC) involving a combination of liquid organic fertilizer (LOF), botanical pesticide, and a bacterial consortium to control M. incognita infection in Coffea arabica seedlings. The treatments in this study consisted of control, synthetic nematicide with the active ingredient fluopyram, and various FBC concentrations ranging from 20%, 40%, 60%, 80%, and 100%. The results demonstrated that the application of 60% to 100% FBC increased plant growth. The most effective and efficient treatment for increasing plant growth was the application of 60% FBC. When compared with control plants, 60% FBC treatment resulted in 6.8% longer root, 9.5% higher plant, 5.3% heavier plant fresh weight, and 4.8% heavier root fresh weight. We also found that the application of 60% to 100% FBC increased the amount of chlorophyll in leaves. FBC application also reduced the number of nematodes in the soil up to 60.6%, the number of galls up to 286.4%, and the severity of root damage up to 118.2%. This study indicates that the application of 60% FBC is the most effective and efficient in controlling M. incognita and stimulating the growth of C. arabica seedlings. Key words: Bacillus; pseudomonas; endophyte; rhizobacteria; fluopyram.

Objective: The present study aimed to isolate and examine the characteristics of endophytic bacteria from kenikir (Cosmos caudatus Kunth.) leaves, to determine the endophytic bacteria capable of producing flavonoids, and to test their potency as anticancer and antimicrobial.Methods: The isolation of endophytic bacteria from C. caudatus Kunth. leaf was conducted by technical surface sterilization. Each of these isolates was produced from the insulation and then cultured on a liquid medium of 0.1% soluble starch, 0.5% peptone, and 0.15% yeast extract with a pH of 7 which have been incubated in room temperature for 5 days with agitation of 120 rpm and extracted with ethyl acetate solvent. The extract was then tested for flavonoid content using thin-layer chromatography method. The anticancer ability of flavonoids was tested by brine shrimp lethality test (BSLT) method, while that for antimicrobial, the test used paper disc method.Results: A total of 15 isolates of endophytic bacteria were successfully isolated from the kenikir leaves, but only 4 isolates produced flavonoids, among others PKM 1 (Serratia sp.), PKM 2 (Neisseria sp.), PKM 17 (Acinetobacter sp.), and PKM 19 (Yersinia sp.). BSLT test results showed that the flavonoids cause mortality in Artemia salina leach with LC50 as much as 16.736 in PKM 17, 17.267 in PKM 2, 18.672 in PKM 1, and 23.411 in PKM 19. The flavonoids also inhibited the growth of pathogens in human-based antimicrobial test results.Conclusion: Flavonoids produced by four endophytic bacterial isolates from kenikir leaves have great potential as anticancer and antimicrobial.

Arbuscular mycorrhizal fungi (AMF) play a role in suppressing the nematode Pratylenchus coffeae. Mycorrhizal helper bacteria (MHB) can increase the effectiveness of AMF to control the diseases. The experimental purpose was to increase the spore population of AMF Glomus spp. in zeolite-based formulation inoculated with liquid consortia of Pseudomonas diminuta and Bacillus subtilis as MHB. The experimental design was a completely random design with six treatments consisted of 10⁶, 10⁷, 10⁸, and 10⁹ CFU/mL MHB liquid inoculants. The control treatments were water and 2% molasses. All treatments were replicated four times. A total of 300 mL/pot Liquid inoculant of MHB have been inoculated a three day before transplanting the maize seedling to the Zeolite inoculated with Glomus spp. in the pot. One month after MHB inoculation, Glomus formulation in Zeolite with different levels of MHB increased the degree of infection. Three months after MHB inoculation, spore content in Zeolite increased. The density of P. diminuta and B. subtilis in zeolite-based mycorrhizal inoculant increased at the end of the experiment. Liquid inoculant MHB contained 10⁸ CFU/mL enhanced spora number fourth times compared to the control. This experiment suggests that P. diminuta and B. subtilis were effective to increase the spore density of AMF inoculant.