Eugenol essential oil (EEO) is the major component in aromatic extracts of Syzygium aromaticum (clove) and has several biological properties, such as antibacterial, antioxidant, and anti-inflammatory activities, as well as controlling vomiting, coughing, nausea, flatulence, diarrhea, dyspepsia, stomach distension, and gastrointestinal spasm pain. It also stimulates the nerves. Therefore, the aim of this study was to extract and purify EEO from clove buds and assess its ability to combat resistant Helicobacter pylori. Additionally, EEO’s anti-inflammatory activity and its ability to suppress H. pylori biofilm formation, which is responsible for antibiotic resistance, was also investigated. Syzygium aromaticum buds were purchased from a local market, ground, and the EEO was extracted by using hydro-distillation and then purified and chemically characterized using gas chromatography–mass spectrometry (GC–MS). A disk-diffusion assay showed that Helicobacter pylori is sensitive to EEO, with an inhibition zone ranging from 10 ± 06 to 22 ± 04 mm. The minimum inhibition concentration (MIC) of EEO ranged from 23.0 to 51.0 μg/mL against both Helicobacter pylori clinical isolates and standard strains. In addition, EEO showed antibiofilm activity at 25 µg/mL and 50 µg/mL against various Helicobacter pylori strains, with suppression percentages of 49.32% and 73.21%, respectively. The results obtained from the anti-inflammatory assay revealed that EEO possesses strong anti-inflammatory activity, with human erythrocyte hemolysis inhibition percentages of 53.04, 58.74, 61.07, and 63.64% at concentrations of 4, 8, 16, and 32 μg/L, respectively. GC–MS analysis revealed that EEO is a major component of Syzygium aromaticum when extracted with a hydro-distillation technique, which was confirmed by its purification using a chemical separation process. EEO exhibited antibacterial action against resistant Helicobacter pylori strains, as well as antibiofilm and anti-inflammatory activities, and is a promising natural alternative in clinical therapy.

Zinc oxide nanoparticles (ZnO-NPs) are regarded as one of the most promising kinds of materials in a variety of fields, including agriculture. Therefore, this study aimed to biosynthesize and characterize ZnO-NPs and evaluate their different biological activities. Seven isolates of actinomycetes were obtained and screened for ZnO-NPs synthesis. The isolate MK-104 was chosen and identified as the Streptomyces plicatus MK-104 strain. The biosynthesized ZnO-NPs exhibited an absorbance peak at 350 nm and were spherical in shape with an average size of 21.72 ± 4.27 nm under TEM. XRD and DLS methods confirmed these results. The biosynthesized ZnO-NPs demonstrated activity against plant pathogenic microbes such as Erwinia amylovora, Aspergillus flavus, Aspergillus niger, Fusarium oxysporum, Fusarium moniliform and Alternaria alternata, with MIC values ranging from 15.6 to 500 µg/mL. Furthermore, ZnO-NPs had a significant effect on Meloidogyne incognita, with death percentages of 88.2, 93.4 and 96.72% after 24, 48 and 72 h of exposure, respectively. Vicia faba seeds were treated with five concentrations of ZnO-NPs (12.5, 25, 50, 100 and 200 µg/mL). Low-moderate ZnO-NP concentrations (12.5–50 µg/mL) were shown to promote seed germination and seedling development, while the mitotic index (MI) decreased as the dosage of ZnO-NPs increased. Micronuclei (MNs) and the chromosomal abnormality index increased as well.

The extensive use of chemical fertilizers is responsible for numerous environmental problems including low food quality, soil degradation, and toxicity to beneficial living organisms in the soil. Nano-fertilizers (NFs) application may be a promising solution for combat these challenges. The current study focused on the efficiency of applying small amounts of NFs incorporated with conventional nitrogen, phosphorus, and potassium (NPK) fertilizers to reduce the quantities of conventional fertilizers (CFs) in lettuce cultivated in sandy soil. This study evaluated the effect of these incorporations on plant growth, yield, phytochemical accumulation, leaf nutrient, and leaf nitrate. A pot experiment was conducted during the winter seasons of 2020/2021 and 2021/2022 using the following treatments: CF100: 100% CFs, CF75NF25: 75% CFs + 25% NFs, CF50NF50: 50% CFs + 50% NFs, CF25NF75: 25% CFs + 75% NFs, and NF100: 100% NFs (=10% of CFs). Our findings displayed that the CF75NF25 and CF50NF50 treatments recorded the highest plant growth parameter values (plant length, root length, number of leaves, and fresh and dry biomass). The maximum of chlorophyll fluorescence measurements (photosystem II efficiency) were obtained in plants fertilized with CF75NF25, followed by CF50NF50 and CF100. The improvement ratios of photosynthetic pigments (Chlorophyll (Chl) a, b, and total) for CF75NF25 were 23.77, 50, and 23.72% in the first season and 10.10, 51.0, and 24.90% in the second season for Chl a, b, and total, respectively, as compared with the CF100 treatment. A similar tendency was observed for the CF50NF50 treatment. Generally, CF75NF25 significantly raised the content of total phenolic compounds (TPC), total flavonoid content (TFC), and antioxidant activity (AOA) in lettuce plants by 36.09, 47.82, and 40.16% in the first season and by 30.39, 37.53, and 32.43% in the second season, respectively, compared with CF100. In addition, the levels of leaf nutrient content and uptake of N, P, and K were significantly higher in plants fertilized with CF75NF25 compared to the other treatments, whereas CF25NF75 had the lowest values among the different treatments across both seasons for most of the tested traits. The nitrate content in lettuce leaves (NO₃⁻) for both seasons was lower than the acceptable level for human consumption. These results indicate that incorporating a low concentration of NFs into CFs could be a promising strategy to reduce the amount of CFs to 75% or 50% of lettuce NPK requirements without significant adverse effects on the growth and productivity of lettuce plants cultivated in sandy soil.

Due to the misuse of antibiotics, the multidrug-resistant Staphylococcus aureus (MDRSA) has caused serious infections and become more difficult to deal with. Here we propose to synthesise copper oxide nanoparticles (CuO-NPs) using a cell-free filter of Streptomyces rochei to enhance antibiotics activity against (MDRSA) and kill them. Characterisation of CuO-NPs using ultraviolet, dynamic light scattering, zeta potential, transmission electron microscopic (TEM), and X-ray diffraction, were investigated. The antibacterial action of the CuO-NPs was tested against standard strain and clinical isolates using the agar well diffusion method and the microdilution assay. The results showed the monodispersed spherical shape CuO-NPs with a mean diameter of 10.7 nm and were found to be active against (MDRSA). By a combination of CuO-NPs with different antibiotics, the highest synergistic effect was observed with cefoxitin, the minimum inhibitory concentration (MIC) was reduced to 6.5 for CuO-NPs, and 19.5 for cefoxitin. Time-kill assay showed the highest reduction in log10 colony-forming unit (CFU)/ml of initial inoculum of MRSA after 24 h. The HFB-4 cells cultured in the presence of CuO-NPs showed normal morphology with 100% viability at 8 µg/ml. TEM showed that combination (1/4 MIC cefoxitin +1/16 MIC CuO-NPs) highly damages bacterial cells’ shape. The biosynthesis CuO-NPs showed antibacterial activity against S. aureus suggesting a promising alternative in clinical.

The increased emergence of multidrug-resistant Helicobacter pylori is related to many health issues. Zingiber officinale (Z. officinale) is a plant usually used in folk medicine to treat a variety of diseases. This study was conducted to evaluate the ability of Z. officinale extract to combat resistant H. pylori. The disc diffusion, microdilution, and microplate assays were performed to evaluate the susceptibility to antibiotics and the antibacterial and antibiofilm activities of the Z. officinale extracts. Using the checkerboard method, the combined effects of gentamicin and Z. officinale extract were investigated. In addition, anti-inflammatory activity and GC-MS analysis were performed according to a modified protocol. According to the findings, H. pylori isolates exhibited resistance rates of 56.33, 50.0, and 45.85 against metronidazole, gentamicin, and tetracycline, respectively. The methanolic extract of Z. officinale showed the strongest effectiveness against resistant H. pylori isolates with MICs of 20.0 to 50.0 µg/mL, including both H. pylori isolates and the standard strain NCTC 11637. Z. officinale extract suppresses the biofilm formed by H. pylori isolates with a percentage of 92.96% at 50.0 µg/mL, compared with 97.19% for gentamicin at the same concentration. According to FICI values, the combination of methanolic Z. officinale extract with gentamicin increases bacterial sensitivity to such drugs. Moreover, the Z. officinale extract exhibits strong anti-inflammatory activity, with inhibition of red blood cell membrane stabilization increasing from 49.83% to 61.47% at a concentration of 4 to 32 µg/mL. The GC-MS analysis of Z. officinale extract exhibits 17 different chemical compounds. Besides showing antibacterial properties, the extract also contains the anti-inflammatory compound gingerol as the main constituent, which inhibits the growth of H. pylori and its biofilm and is a promising natural therapeutic alternative or enhances antibiotic activity.

Colistin (polymyxin E) is a bactericidal antibiotic used to treat severe infections caused by multidrug-resistant Gram-negative bacteria. The product of the mcr1 gene generates transferrable plasmi...