In the last few decades, the search for metal nanoparticles as an alternative to cancer treatments and antibiotics has increased. In this article, the spectroscopic (ultraviolet–visible (UV-vis), electron-dispersing X-ray (EDX), and Fourier transform infrared (FT-IR)), microscopic (field emission scanning electron microscope (FE-SEM), transmission electron microscope (TEM), and atomic force microscope (AFM)), structural (X-ray diffractometer (XRD) and zetasizer), and analytic (thermogravimetric/differential thermal analyzer (TGA-DTA)) characterization of the silver nanoparticles (AgNPs) produced from Papaver rhoeas (PR) L. leaf extract are presented. PR-AgNPs are generally spherical and have a maximum surface plasmon resonance of 464.03 nm. The dimensions of the manufactured nanomaterial are in the range of 1.47–7.31 nm. PR-AgNPs have high thermal stability and a zeta potential of −36.1 mV. The minimum inhibitory concentration (MIC) values (mg L⁻¹) of PR-AgNPs on Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans are 1.50, 0.75, 3.00, 6.00, and 0.37, respectively. In the study, the cytotoxic and proliferative effects of PR-AgNPs using the MTT (3-(4,5-dimethylthiazol-2-yl)-diphenyltetrazolium bromide) method on various cancer cell lines (CACO-2 (human colon adenocarcinoma cell), MCF-7 (human breast cancer cell), T98-G (glioblastoma multiforme cell), and healthy HUVEC (human umbilical vein endothelial cell)) cell lines are presented. After 24 and 48 h of the application, the half-maximum inhibitory concentration (IC₅₀) values (μg mL⁻¹) of PR-AgNPs on HUVEC, CACO-2, MCF-7, and T98-G lines are 2.365 and 2.380; 2.526 and 2.521; 3.274 and 3.318; 3.472 and 3.526, respectively. Comprehensive in vivo research of PR-AgNPs is proposed to reveal their potential for usage in sectors such as nanomedicine and nanochemistry.

Bu çalışmada, gümüş nanopartiküller (AgNP’ler) biyolojik yöntemle kolay, düşük maliyetli ve çevre dostu bir şekilde elde edilmiştir. Sentezi yapılan AgNP’lerin karakterizasyonu UV-visible Spektrofotometre (UV-Vis.), Alan Emisyon Taramalı Elektron Mikroskobu (FE-SEM), Transmisyon Elektron Mikroskobu (TEM), Enerji Dağılımlı X-Işını Spektroskopisi (EDX), X- Işını Kırınımı Difraktrometresi (XRD), Fourier Dönüşümü Kızılötesi Spektroskopisi (FT-IR) ve Zeta boyut ve potansiyel cihazı kullanılarak yapılmıştır. AgNP’lerin 440-450 nm absorbansta maksimum pik vermiştir. TEM ve FE-SEM sonuçlarına göre nanopartiküllerin morfolojik yapılarının küresel ve ortalama 38 nm; XRD sonuçlarına göre nanopartiküllerin toz kristal yapılarının kübik ve 21.94 nm boyutunda; zeta boyutunun ise ortalama 158.2 nm, zeta potansiyelinin -23.4 mV olduğu görülmüştür. Sentezlenen AgNP'lerin Staphylococcus aureus ATCC 29213, Bacillus subtilis ATCC 11774 Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 ve Candida albicans üzerinde Minimum İnhibisyon Konsantrasyonları (MİK) (mg/mL) sırasıyla 1.25, 0.625, 2.50, 1.25 ve 0.312 olarak tespit edilmiştir. Söz konusu mikroorganizmaların AgNO3 ve antibiyotik MİK değerleri (mg/mL) sırasıyla 2.65;2, 1.32;1, 0.66;2, 1.32;4, 0.66;2 olarak tespit edilmiştir.

Bu çalışmada, Abelmoschus esculentus (AE) yaprağı özütü kullanılarak düşük maliyetli, çevre dostu ve kolay bir şekilde gümüş nanopartiküller (AgNP’ler) sentezlendi. Ultraviyole-görünür (UV-vis.) Spektrofotometre analiz verilerine göre, nanokristaller 431 nm'de karakteristik bir tepe noktası gösterdi. Taramalı Elektron Mikroskobu (SEM), Transmisyon Elektron Mikroskobu (TEM) ve enerji dağılımlı X-Işını Spektroskopisi (EDX) analizleri, biyolojik yolla sentezlenen AgNP'lerin morfolojik yapılarının küresel olduğunu ortaya koydu. XRD analizi sonuçlarına göre AgNP'lerin kristal yapılarının kübik olduğu görüldü. Nanopartiküllerin boyutu, Debye-Scherrer denklemi ile 19.05 nm olarak hesaplandı. Sentezlenen nanomalzemenin zeta potansiyeli -9.83 mV ve zeta boyutu da 142.2 nm olarak ölçüldü. AgNP'lerin, gıda patojenlerinden Staphylococcus aureus ATCC 29213, Escherichia coli ATCC25922, Bacillus subtilis ATCC11774, Pseudomonas aeruginosa ATCC27833 ve Candida albicans üzerindeki Minimum İnhibitör Konsantrasyonu (MİK) değerlerinin (mg/mL) sırasıyla 0.125, 0.50, 0.0312, 0.125 ve 0.125 düzeyinde olduğu tespit edildi.

In this study, the contamination levels of Aflatoxin M1 of 248 sterilized/UHT milk, Beyaz Cheese, Cecil Cheese, Cokelek, Cream Cheese, Lor Cheese, Van Otlu (herbed) Cheese, Van Otlu Lavash Cheese and butter samples from randomly selected market and supermarkets from Diyarbakir province center were determined by ELISA method. The average AFM1 contamination value of the cheese and butter samples investigated was 133.51 ng/kg; while the minimum and maximum values were 50 and 595.31 ng/kg, respectively. On the other hand, the average AFM1 value of 13 sterilized milk samples was 52.59 ng/kg; minimum and maximum values were determined as 25.97 and 80.0 ng/kg, respectively. In the study, the AFM1 results of 34 samples (13.7%) of sterilized milk, Cecil Cheese, Cokelek, Cream Cheese, Lor Cheese, Van Otlu Cheese, Van Otlu Lavash Cheese and butter samples were exceeded the limits set by the European Commission for AFM1 (50 and 250 ng/kg). The results of the analysis show that the milk and dairy products those were offered for consumption in Diyarbakir had a limited potential for consumers in terms of AFM1.

This study was carried out to synthesize silver nanoparticles (AgNPs) from Arum dioscoridis (AD) leaf extract and to investigate the cytotoxic and antipathogenic effects of them. The plant material had a reducing and stabilizing effect on the synthesized nanomaterial. During the plant-mediated synthesis of nanomaterials, no substances that would cause environmental pollution were used. For the structural characterization of AD-AgNPs, Ultraviolet-visible (UV-vis) Spectroscopy, Field Emission Scanning Electron Microscopy (FE-SEM), Electron Dispersive X-ray (EDX) Spectroscopy, Fourier Transform Infrared (FT-IR) Spectroscopy, Transmission Electron Microscopy (TEM), X-ray Diffractometer (XRD), Atomic Force Microscopy (AFM) and Zetasizer analyses were performed. The produced AgNPs showed maximum surface plasmon resonance at 431.67 nm and had mostly spherical morphology. The zeta potential value of the nanomaterial was -9.76 mV and the average powder crystal size was 31.48 nm. The minimum inhibitory concentration (MIC) values (mg/L) of AD-AgNPs on Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans were 0.25, 2.00, 0.125, 4.00, and 1.00, respectively. After 24 and 48 hours of application by MTT [3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromid] assay, the half-maximal inhibitory concentrations (IC50: μg/mL) of AD-AgNPs on human colon adenocarcinoma cell (CACO-2), human breast cancer cell (MCF-7), glioblastoma multiforme cell (T98-G), and healthy human umbilical vein endothelial cell (HUVEC) lines were determined as 2.977, 2.801, 5.694, 4.392; 2.115, 2.300, 2.612, 4.091, respectively.