Raman spectroscopy is a useful method in biological, biomedical, food, and agricultural studies, allowing the simultaneous examination of various chemical compounds and evaluation of molecular changes occurring in tested objects. The purpose of our research was to explain how the elimination of ω-fractions from the wheat gliadin complex influences the secondary structures of the remaining αβγ-gliadins. To this aim, we analyzed the endosperm of wheat kernels as well as gliadin proteins extracted from two winter wheat genotypes: wasko.gl+ (control genotype containing the full set of gliadins) and wasko.gl− (modified genotype lacking all ω-gliadins). Based on the decomposition of the amide I band, we observed a moderate increase in β-forms (sheets and turns) at the expense of α-helical and random coil structures for gliadins isolated from the flour of the wasko.gl− line. Since ω-gliadins contain no cysteine residues, they do not participate in the formation of the disulfide bridges that stabilize the protein structure. However, they can interact with other proteins via weak, low-energetic hydrogen bonds. We conclude that the elimination of ω-fractions from the gliadin complex causes minor modifications in secondary structures of the remaining gliadin proteins. In our opinion, these small, structural changes of proteins may lead to alterations in gliadin allergenicity.

Remote sensing methods based on UAV and hand-held devices as well have been used to assess the response to nitrogen and sulfur fertilization of hypoallergenic genotypes of winter wheat. The field experiment was conducted using the split-split-plot design with three repetitions. The first factor was the two genotypes of winter wheat specified as V1 (without allergic protein) and V2 (with allergic protein), and the second factor was three doses of sulfur fertilization: 0, 20 and 40 kg S per ha. The third factor consisted of six doses of nitrogen fertilization: 0, 40, 60, 80, 100 and 120 kg N·ha⁻¹. Monitoring the values of the indicators depending on the level of nitrogen and sulfur fertilization allowed the results to be used in yield forecasting, assessment of plant condition, LAI value, nutritional status in the cultivation of wheat. The maximum yield should be expected at doses of 94 and 101 kg N ha⁻¹ for genotypes V1 and V2, respectively, giving yields of 5.39 and 4.71 Mg ha⁻¹. On the basis of the tested vegetation indices, the highest doses of N should be applied using the normalized difference RedEdge (NDRE), and the lowest ones based on the enhanced vegetation index (EVI), and, in the latter case, a reduction in yield of more than 200 kg ha⁻¹ in the V2 genotype should be taken into account.