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Ziad Fajloun
Department of Biology, Faculty of Sciences, Section III, Michel Slayman Tripoli Campus, Lebanese University, Ras Maska 1352, Lebanon

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Journal article
Published: 14 July 2021 in Sci
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In this work, we pursued the biological characterization of the venom of Montivipera bornmuelleri, a viper from the Lebanese mountains. In relation to its antibacterial potential, the inhibitory effect of this venom on the F1F0-ATPase enzymes of Gram-positive Staphylocoocus epidermidis and Gram-negative Escherichia coli bacteria was examined. In order to determine the degree of cytotoxicity of the venom on the HCT116 human colon cancer cell lines, the biological MTT proliferation and cell viability test were implemented. After validation of the enzymatic F1F0-ATPase model by the spectrophotometric method, using quercetin as the reference ligand, results revealed that M. bornmuelleri venom is able to inhibit the activity of the enzyme of these two bacteria with a concentration of the order of 100–150 µg/mL. In addition, a venom concentration of 10 µg/mL was sufficient to kill the totality of HCT116 cell lines cultivated in vitro. These data show that M. bornmuelleri venom is a mixture of diverse molecules presenting activities of interest, and is a potential source to explore in order to discover new drug candidates.

ACS Style

Milena Kfoury; Charbel Mouawad; Mariam Rifi; Riyad Sadek; Jean-Marc Sabatier; Hala Nehme; Ziad Fajloun. Montivipera bornmuelleri Venom: Inhibitory Effect on Staphylococcus epidermidis and Escherichia coli F1F0-ATPases and Cytotoxicity on HCT116 Cancer Cell Lines. Sci 2021, 3, 31 .

AMA Style

Milena Kfoury, Charbel Mouawad, Mariam Rifi, Riyad Sadek, Jean-Marc Sabatier, Hala Nehme, Ziad Fajloun. Montivipera bornmuelleri Venom: Inhibitory Effect on Staphylococcus epidermidis and Escherichia coli F1F0-ATPases and Cytotoxicity on HCT116 Cancer Cell Lines. Sci. 2021; 3 (3):31.

Chicago/Turabian Style

Milena Kfoury; Charbel Mouawad; Mariam Rifi; Riyad Sadek; Jean-Marc Sabatier; Hala Nehme; Ziad Fajloun. 2021. "Montivipera bornmuelleri Venom: Inhibitory Effect on Staphylococcus epidermidis and Escherichia coli F1F0-ATPases and Cytotoxicity on HCT116 Cancer Cell Lines." Sci 3, no. 3: 31.

Review
Published: 15 June 2021 in Antibiotics
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Honeybees are one of the most marvelous and economically beneficial insects. As pollinators, they play a vital role in every aspect of the ecosystem. Beehive products have been used for thousands of years in many cultures for the treatment of various diseases. Their healing properties have been documented in many religious texts like the Noble Quran and the Holy Bible. Honey, bee venom, propolis, pollen and royal jelly all demonstrated a richness in their bioactive compounds which make them effective against a variety of bacterial strains. Furthermore, many studies showed that honey and bee venom work as powerful antibacterial agents against a wide range of bacteria including life-threatening bacteria. Several reports documented the biological activities of honeybee products but none of them emphasized on the antibacterial activity of all beehive products. Therefore, this review aims to highlight the antibacterial activity of honey, bee venom, propolis, pollen and royal jelly, that are produced by honeybees.

ACS Style

Rita Nader; Rawan Mackieh; Rim Wehbe; Dany El Obeid; Jean Sabatier; Ziad Fajloun. Beehive Products as Antibacterial Agents: A Review. Antibiotics 2021, 10, 717 .

AMA Style

Rita Nader, Rawan Mackieh, Rim Wehbe, Dany El Obeid, Jean Sabatier, Ziad Fajloun. Beehive Products as Antibacterial Agents: A Review. Antibiotics. 2021; 10 (6):717.

Chicago/Turabian Style

Rita Nader; Rawan Mackieh; Rim Wehbe; Dany El Obeid; Jean Sabatier; Ziad Fajloun. 2021. "Beehive Products as Antibacterial Agents: A Review." Antibiotics 10, no. 6: 717.

Journal article
Published: 14 April 2021 in Molecules
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Colon carcinogenesis is ranked second globally among human diseases after cardiovascular failures. Bee venom (BV) has been shown to possess in vitro anticancer effects against several types of cancer cells. The two main biopeptides of Apis mellifera BV, namely, melittin (MEL) and phospholipase A2 (PLA2), are suspected to be the biomolecules responsible for the anticancer activity. The present work aims to evaluate the cytotoxic effect of the A. mellifera venom on human colon carcinoma cells (HCT116), and to assess the synergistic effect of MEL and PLA2 on these cells. After analyzing, through high-pressure liquid chromatography, the proportions of MEL and PLA2 on BV, we have established a cell viability assay to evaluate the effect of BV, MEL, PLA2, and a mixture of MEL and PLA2 on the HCT116 cells. Results obtained showed a strong cytotoxicity effect induced by the A. mellifera venom and to a lower extent MEL or PLA2 alone. Remarkably, when MEL and PLA2 were added together, their cytotoxic effect was greatly improved, suggesting a synergistic activity on HCT116 cells. These findings confirm the cytotoxic effect of the A. mellifera venom and highlight the presence of synergistic potential activities between MEL and PLA2, possibly inducing membrane disruption of HCT116 cancer cells. Altogether, these results could serve as a basis for the development of new anticancer treatments.

ACS Style

Carole Yaacoub; Mariam Rifi; Dany El-Obeid; Hiba Mawlawi; Jean-Marc Sabatier; Bruno Coutard; Ziad Fajloun. The Cytotoxic Effect of Apis mellifera Venom with a Synergistic Potential of Its Two Main Components—Melittin and PLA2—On Colon Cancer HCT116 Cell Lines. Molecules 2021, 26, 2264 .

AMA Style

Carole Yaacoub, Mariam Rifi, Dany El-Obeid, Hiba Mawlawi, Jean-Marc Sabatier, Bruno Coutard, Ziad Fajloun. The Cytotoxic Effect of Apis mellifera Venom with a Synergistic Potential of Its Two Main Components—Melittin and PLA2—On Colon Cancer HCT116 Cell Lines. Molecules. 2021; 26 (8):2264.

Chicago/Turabian Style

Carole Yaacoub; Mariam Rifi; Dany El-Obeid; Hiba Mawlawi; Jean-Marc Sabatier; Bruno Coutard; Ziad Fajloun. 2021. "The Cytotoxic Effect of Apis mellifera Venom with a Synergistic Potential of Its Two Main Components—Melittin and PLA2—On Colon Cancer HCT116 Cell Lines." Molecules 26, no. 8: 2264.

Review
Published: 12 April 2021 in Molecules
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Cardiovascular diseases (CVDs) are considered as a major cause of death worldwide. Therefore, identifying and developing therapeutic strategies to treat and reduce the prevalence of CVDs is a major medical challenge. Several drugs used for the treatment of CVDs, such as captopril, emerged from natural products, namely snake venoms. These venoms are complex mixtures of bioactive molecules, which, among other physiological networks, target the cardiovascular system, leading to them being considered in the development and design of new drugs. In this review, we describe some snake venom molecules targeting the cardiovascular system such as phospholipase A2 (PLA2), natriuretic peptides (NPs), bradykinin-potentiating peptides (BPPs), cysteine-rich secretory proteins (CRISPs), disintegrins, fibrinolytic enzymes, and three-finger toxins (3FTXs). In addition, their molecular targets, and mechanisms of action—vasorelaxation, inhibition of platelet aggregation, cardioprotective activities—are discussed. The dissection of their biological effects at the molecular scale give insights for the development of future snake venom-derived drugs.

ACS Style

Jacinthe Frangieh; Mohamad Rima; Ziad Fajloun; Daniel Henrion; Jean-Marc Sabatier; Christian Legros; César Mattei. Snake Venom Components: Tools and Cures to Target Cardiovascular Diseases. Molecules 2021, 26, 2223 .

AMA Style

Jacinthe Frangieh, Mohamad Rima, Ziad Fajloun, Daniel Henrion, Jean-Marc Sabatier, Christian Legros, César Mattei. Snake Venom Components: Tools and Cures to Target Cardiovascular Diseases. Molecules. 2021; 26 (8):2223.

Chicago/Turabian Style

Jacinthe Frangieh; Mohamad Rima; Ziad Fajloun; Daniel Henrion; Jean-Marc Sabatier; Christian Legros; César Mattei. 2021. "Snake Venom Components: Tools and Cures to Target Cardiovascular Diseases." Molecules 26, no. 8: 2223.

Journal article
Published: 01 January 2021 in Venoms and Toxins
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Phospholipases A2 enzymes are found in many mammalian tissues and in animal venoms. Those present in bee venom (bvPLA2) and snake venom (svPLA2) have been studied more particularly for their biological activities of interest. Although they belong to the same family of secreted PLA2 (sPLA2), bvPLA2 and svPLA2 differ from a structural and functional point of view. In this review, we describe the morphological characteristics of these two enzymes and the structural determinants that govern their functions. After describing their cytotoxicity, we compared their biological activities, including antimicrobial, anti-tumor, anti-inflammatory, anti-neurodegenerative, and anti-nociceptive effects. In addition, we highlighted their therapeutical applications and deduced bvPLA2 has better potential than svPLA2 in biotechnological and pharmaceutical innovations.

ACS Style

Lara Bitar; Dania Jundi; Mohamad Rima; Jean-Marc Sabatier; Ziad Fajloun. Bee Venom PLA2 Versus Snake Venom PLA2: Evaluation of Structural and Functional Properties. Venoms and Toxins 2021, 1, 1 -12.

AMA Style

Lara Bitar, Dania Jundi, Mohamad Rima, Jean-Marc Sabatier, Ziad Fajloun. Bee Venom PLA2 Versus Snake Venom PLA2: Evaluation of Structural and Functional Properties. Venoms and Toxins. 2021; 1 ():1-12.

Chicago/Turabian Style

Lara Bitar; Dania Jundi; Mohamad Rima; Jean-Marc Sabatier; Ziad Fajloun. 2021. "Bee Venom PLA2 Versus Snake Venom PLA2: Evaluation of Structural and Functional Properties." Venoms and Toxins 1, no. : 1-12.

Journal article
Published: 18 November 2020 in Antibiotics
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Bacterial resistance has become a worrying problem for human health, especially since certain bacterial strains of Escherichia coli (E. coli) can cause very serious infections. Thus, the search for novel natural inhibitors with new bacterial targets would be crucial to overcome resistance to antibiotics. Here, we evaluate the inhibitory effects of Apis mellifera bee venom (BV-Am) and of its two main components -melittin and phospholipase A2 (PLA2)- on E. coli F1F0-ATPase enzyme, a crucial molecular target for the survival of these bacteria. Thus, we optimized a spectrophotometric method to evaluate the enzymatic activity by quantifying the released phosphate from ATP hydrolysis catalyzed by E. coli F1F0-ATPase. The protocol developed for inhibition assays of this enzyme was validated by two reference inhibitors, thymoquinone (IC50 = 57.5 μM) and quercetin (IC50 = 30 μM). Results showed that BV-Am has a dose-dependent inhibitory effect on E. coli F1F0-ATPase with 50% inhibition at 18.43 ± 0.92 μg/mL. Melittin inhibits this enzyme with IC50 = 9.03 ± 0.27 µM, emphasizing a more inhibitory effect than the two previous reference inhibitors adopted. Likewise, PLA2 inhibits E. coli F1F0-ATPase with a dose-dependent effect (50% inhibition at 2.11 ± 0.11 μg/mL) and its combination with melittin enhanced the inhibition extent of this enzyme. Crude venom and mainly melittin and PLA2, inhibit E. coli F1F0-ATPase and could be considered as important candidates for combating resistant bacteria.

ACS Style

Hala Nehme; Helena Ayde; Dany El Obeid; Jean Marc Sabatier; Ziad Fajloun. Potential Inhibitory Effect of Apis mellifera’s Venom and of Its Two Main Components—Melittin and PLA2—on Escherichia coli F1F0-ATPase. Antibiotics 2020, 9, 824 .

AMA Style

Hala Nehme, Helena Ayde, Dany El Obeid, Jean Marc Sabatier, Ziad Fajloun. Potential Inhibitory Effect of Apis mellifera’s Venom and of Its Two Main Components—Melittin and PLA2—on Escherichia coli F1F0-ATPase. Antibiotics. 2020; 9 (11):824.

Chicago/Turabian Style

Hala Nehme; Helena Ayde; Dany El Obeid; Jean Marc Sabatier; Ziad Fajloun. 2020. "Potential Inhibitory Effect of Apis mellifera’s Venom and of Its Two Main Components—Melittin and PLA2—on Escherichia coli F1F0-ATPase." Antibiotics 9, no. 11: 824.

Journal article
Published: 27 October 2020 in PeerJ
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Background Montivipera bornmuelleri’s venom has shown immunomodulation of cytokines release in mice and selective cytotoxicity on cancer cells in a dose-dependent manner, highlighting an anticancer potential. Here, we extend these findings by elucidating the sensitivity of murine B16 skin melanoma and 3-MCA-induced murine fibrosarcoma cell lines to M. bornmuelleri’s venom and its effect on tumor growth in vivo. Methods The toxicity of the venom on B16 and MCA cells was assessed using flow cytometry and xCELLigence assays. For in vivo testing, tumor growth was followed in mice after intratumoral venom injection. Results The venom toxicity showed a dose-dependent cell death on both B16 and MCA cells. Interestingly, overexpression of ovalbumin increased the sensitivity of the cells to the venom. However, the venom was not able to eradicate induced-tumor growth when injected at 100 µg/kg. Our study demonstrates a cytotoxic effect of M. bornmuelleri’s venom in vitro which, however, does not translate to an anticancer action in vivo.

ACS Style

Carol Haddoub; Mohamad Rima; Sandrine Heurtebise; Myriam Lawand; Dania Jundi; Riyad Sadek; Sebastian Amigorena; Ziad Fajloun; Marc C. Karam. Cytotoxic effect of Montivipera bornmuelleri’s venom on cancer cell lines: in vitro and in vivo studies. PeerJ 2020, 8, e9909 .

AMA Style

Carol Haddoub, Mohamad Rima, Sandrine Heurtebise, Myriam Lawand, Dania Jundi, Riyad Sadek, Sebastian Amigorena, Ziad Fajloun, Marc C. Karam. Cytotoxic effect of Montivipera bornmuelleri’s venom on cancer cell lines: in vitro and in vivo studies. PeerJ. 2020; 8 ():e9909.

Chicago/Turabian Style

Carol Haddoub; Mohamad Rima; Sandrine Heurtebise; Myriam Lawand; Dania Jundi; Riyad Sadek; Sebastian Amigorena; Ziad Fajloun; Marc C. Karam. 2020. "Cytotoxic effect of Montivipera bornmuelleri’s venom on cancer cell lines: in vitro and in vivo studies." PeerJ 8, no. : e9909.

Review
Published: 21 May 2020 in Molecules
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The inappropriate or excessive use of antimicrobial agents caused an emerging public health problem due to the resulting resistance developed by microbes. Therefore, there is an urgent need to develop effective antimicrobial strategies relying on natural agents with different mechanisms of action. Nature has been known to offer many bioactive compounds, in the form of animal venoms, algae, and plant extracts that were used for decades in traditional medicine. Animal venoms and secretions have been deeply studied for their wealth in pharmaceutically promising molecules. As such, they were reported to exhibit many biological activities of interest, such as antibacterial, antiviral, anticancer, and anti-inflammatory activities. In this review, we summarize recent findings on the antimicrobial activities of crude animal venoms/secretions, and describe the peptides that are responsible of these activities.

ACS Style

Tania Yacoub; Mohamad Rima; Marc Karam; Jean-Marc Sabatier; Jean-Marc Sabatier And Ziad Fajloun. Antimicrobials from Venomous Animals: An Overview. Molecules 2020, 25, 2402 .

AMA Style

Tania Yacoub, Mohamad Rima, Marc Karam, Jean-Marc Sabatier, Jean-Marc Sabatier And Ziad Fajloun. Antimicrobials from Venomous Animals: An Overview. Molecules. 2020; 25 (10):2402.

Chicago/Turabian Style

Tania Yacoub; Mohamad Rima; Marc Karam; Jean-Marc Sabatier; Jean-Marc Sabatier And Ziad Fajloun. 2020. "Antimicrobials from Venomous Animals: An Overview." Molecules 25, no. 10: 2402.

Review
Published: 19 August 2019 in Molecules
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Apitherapy is an alternate therapy that relies on the usage of honeybee products, most importantly bee venom for the treatment of many human diseases. The venom can be introduced into the human body by manual injection or by direct bee stings. Bee venom contains several active molecules such as peptides and enzymes that have advantageous potential in treating inflammation and central nervous system diseases, such as Parkinson’s disease, Alzheimer’s disease, and amyotrophic lateral sclerosis. Moreover, bee venom has shown promising benefits against different types of cancer as well as anti-viral activity, even against the challenging human immunodeficiency virus (HIV). Many studies described biological activities of bee venom components and launched preclinical trials to improve the potential use of apitoxin and its constituents as the next generation of drugs. The aim of this review is to summarize the main compounds of bee venom, their primary biological properties, mechanisms of action, and their therapeutic values in alternative therapy strategies.

ACS Style

Rim Wehbe; Jacinthe Frangieh; Mohamad Rima; Dany El Obeid; Jean-Marc Sabatier; Ziad Fajloun. Bee Venom: Overview of Main Compounds and Bioactivities for Therapeutic Interests. Molecules 2019, 24, 2997 .

AMA Style

Rim Wehbe, Jacinthe Frangieh, Mohamad Rima, Dany El Obeid, Jean-Marc Sabatier, Ziad Fajloun. Bee Venom: Overview of Main Compounds and Bioactivities for Therapeutic Interests. Molecules. 2019; 24 (16):2997.

Chicago/Turabian Style

Rim Wehbe; Jacinthe Frangieh; Mohamad Rima; Dany El Obeid; Jean-Marc Sabatier; Ziad Fajloun. 2019. "Bee Venom: Overview of Main Compounds and Bioactivities for Therapeutic Interests." Molecules 24, no. 16: 2997.

Journal article
Published: 30 March 2019 in Toxins
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Bee venom is a mixture of several components with proven therapeutic benefits, among which are anti-inflammatory, analgesic, and various cardiovascular conditions. In this work, we analyzed for the first time the proteomic content and biological properties of the crude venom from Apis mellifera syriaca, a honeybee from the Middle East region. Using high-performance liquid chromatography-tandem mass spectrometry, we evidence the venom contains phospholipase A2, hyaluronidase, mast cell-degranulating peptide, adolapin, apamin, and melittin. The latter was purified by solid phase extraction method (SPE) and tested in parallel with crude venom for biological activities. Precisely, crude venom-but not melittin-exhibited antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa strains. Alongside, hemolytic activity was observed in human blood subjected to the venom at high doses. A. mellifera syriaca venom displayed antioxidant activities, and not surprisingly, PLA2 catalytic activity. Eventually, the venom proved to exert antiproliferative effects against MCF-7 and 3T3 cancer cells lines. This first report of a new bee venom opens new avenues for therapeutic uses of bee venoms.

ACS Style

Jacinthe Frangieh; Yahya Salma; Katia Haddad; Cesar Mattei; Christian Legros; Ziad Fajloun; Dany El Obeid. First Characterization of The Venom from Apis mellifera syriaca, A Honeybee from The Middle East Region. Toxins 2019, 11, 191 .

AMA Style

Jacinthe Frangieh, Yahya Salma, Katia Haddad, Cesar Mattei, Christian Legros, Ziad Fajloun, Dany El Obeid. First Characterization of The Venom from Apis mellifera syriaca, A Honeybee from The Middle East Region. Toxins. 2019; 11 (4):191.

Chicago/Turabian Style

Jacinthe Frangieh; Yahya Salma; Katia Haddad; Cesar Mattei; Christian Legros; Ziad Fajloun; Dany El Obeid. 2019. "First Characterization of The Venom from Apis mellifera syriaca, A Honeybee from The Middle East Region." Toxins 11, no. 4: 191.

Review
Published: 22 October 2018 in Molecules
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Snake venom serves as a tool of defense against threat and helps in prey digestion. It consists of a mixture of enzymes, such as phospholipase A2, metalloproteases, and l-amino acid oxidase, and toxins, including neurotoxins and cytotoxins. Beside their toxicity, venom components possess many pharmacological effects and have been used to design drugs and as biomarkers of diseases. Viperidae is one family of venomous snakes that is found nearly worldwide. However, three main vipers exist in the Middle Eastern region: Montivipera bornmuelleri, Macrovipera lebetina, and Vipera (Daboia) palaestinae. The venoms of these vipers have been the subject of many studies and are considered as a promising source of bioactive molecules. In this review, we present an overview of these three vipers, with a special focus on their venom composition as well as their biological activities, and we discuss further frameworks for the exploration of each venom.

ACS Style

Mohamad Rima; Seyedeh Maryam Alavi Naini; Marc Karam; Riyad Sadek; Jean-Marc Sabatier; Ziad Fajloun. Vipers of the Middle East: A Rich Source of Bioactive Molecules. Molecules 2018, 23, 2721 .

AMA Style

Mohamad Rima, Seyedeh Maryam Alavi Naini, Marc Karam, Riyad Sadek, Jean-Marc Sabatier, Ziad Fajloun. Vipers of the Middle East: A Rich Source of Bioactive Molecules. Molecules. 2018; 23 (10):2721.

Chicago/Turabian Style

Mohamad Rima; Seyedeh Maryam Alavi Naini; Marc Karam; Riyad Sadek; Jean-Marc Sabatier; Ziad Fajloun. 2018. "Vipers of the Middle East: A Rich Source of Bioactive Molecules." Molecules 23, no. 10: 2721.

Journal article
Published: 19 June 2018 in Stem Cell Research & Therapy
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Human adipose-derived mesenchymal stem cells (hADSCs) are promising cells that may promote hepatocyte differentiation (Hep-Dif) and improve liver function, but the involvement of Cdc42, a key small RhoGTPase which plays a crucial role in aging, is still not well established. We hypothesized that the inhibition of Cdc42 may rescue the hepatogenic potential of hADSCs derived from aged donors. hADSCs isolated from 61 women of different ages were cultured for evaluation of the proliferation of cells, adherence, apoptosis, immunomodulation, immunophenotyping, multipotency, gene expression, and cell function during Hep-Dif. Inhibition of Cdc42 by ML141 was realized during two phases: initiation (days –2 to 14 (D–2/14)) from undifferentiated to hepatoblast-like cells, or maturation (days 14 to 28 (D14/28)) from undifferentiated to hepatocyte-like cells. Mechanistic insights of the Wnt(s)/MAPK/PI3K/miR-122 pathways were studied. Cdc42 activity in undifferentiated hADSCs showed an age-dependent significant increase in Cdc42-GTP correlated to a decrease in Cdc42GAP; the low potentials of cell proliferation, doubling, adherence, and immunomodulatory ability (proinflammatory over anti-inflammatory) contrary to the apoptotic index of the aged group were significantly reversed by ML141. Aged donor cells showed a decreased potential for Hep-Dif which was rescued by ML141 treatment, giving rise to mature and functional hepatocyte-like cells as assessed by hepatic gene expression, cytochrome activity, urea and albumin production, low-density lipoprotein (LDL) uptake, and glycogen storage. ML141-induced Hep-Dif showed an improvement in mesenchymal-epithelial transition, a switch from Wtn-3a/β-catenin to Wnt5a signaling, involvement of PI3K/PKB but not the MAPK (ERK/JNK/p38) pathway, induction of miR-122 expression, reinforcing the exosomes release and the production of albumin, and epigenetic changes. Inhibition of PI3K and miR-122 abolished completely the effects of ML141 indicating that inhibition of Cdc42 promotes the Hep-Dif through a Wnt5a/PI3K/miR-122/HNF4α/albumin/E-cadherin-positive action. The ML141(D–2/14) protocol had more pronounced effects when compared with ML141(D14/28); inhibition of DNA methylation in combination with ML141(D–2/14) showed more efficacy in rescuing the Hep-Dif of aged hADSCs. In addition to Hep-Dif, the multipotency of aged hADSC-treated ML141 was observed by rescuing the adipocyte and neural differentiation by inducing PPARγ/FABP4 and NeuN/O4 but inhibiting Pref-1 and GFAP, respectively. ML141 has the potential to reverse the age-related aberrations in aged stem cells and promotes their hepatogenic differentiation. Selective inhibition of Cdc42 could be a potential target of drug therapy for aging and may give new insights on the improvement of Hep-Dif.

ACS Style

Diana Chaker; Charbel Mouawad; Albert Azar; Didier Quilliot; Ibrahim Achkar; Ziad Fajloun; Nehman Makdissy. Inhibition of the RhoGTPase Cdc42 by ML141 enhances hepatocyte differentiation from human adipose-derived mesenchymal stem cells via the Wnt5a/PI3K/miR-122 pathway: impact of the age of the donor. Stem Cell Research & Therapy 2018, 9, 1 -27.

AMA Style

Diana Chaker, Charbel Mouawad, Albert Azar, Didier Quilliot, Ibrahim Achkar, Ziad Fajloun, Nehman Makdissy. Inhibition of the RhoGTPase Cdc42 by ML141 enhances hepatocyte differentiation from human adipose-derived mesenchymal stem cells via the Wnt5a/PI3K/miR-122 pathway: impact of the age of the donor. Stem Cell Research & Therapy. 2018; 9 (1):1-27.

Chicago/Turabian Style

Diana Chaker; Charbel Mouawad; Albert Azar; Didier Quilliot; Ibrahim Achkar; Ziad Fajloun; Nehman Makdissy. 2018. "Inhibition of the RhoGTPase Cdc42 by ML141 enhances hepatocyte differentiation from human adipose-derived mesenchymal stem cells via the Wnt5a/PI3K/miR-122 pathway: impact of the age of the donor." Stem Cell Research & Therapy 9, no. 1: 1-27.

Journal article
Published: 18 October 2017 in Journal of Venom Research
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Because snake venoms are complex mixtures of bioactive molecules, snake bites produce a large panel of symptoms which cannot be totally prevented by current antivenoms. Thus investigating plant extracts for antivenomics therapy approaches seemed relevant. Here, we evaluated the potency of the aqueous Buds extract of Eucalyptus (ABEE) to counteract the main enzymatic activities of Montivipera bornmuelleri venom. We showed that ABEE efficiently counteracts the proteolytic, Phospholipases A2 (PLA2), and L-aminoacid oxidase activities (LAAO) of M. bornmuelleri venom. ABEE was found to inhibit Acetylcholine esterase (AChE) and to exhibit a potent antioxidant activity. In addition, M. bornmuelleri venom displays antibacterial properties against Staphylococcus aureus, which were not inhibited by ABEE. We also showed that of M. bornmuelleri venom lacks AChE, either anti-AChE activities. ABEE represents a promising natural source of antivenomics compounds against the deleterious effects of M. bornmuelleri or other Vipera species bites.

ACS Style

Joseph Khoury; Ranin Dabbousy; Riyad Sadek; Sayed Antoun; Walid Hleihel; Christian Legros; Ziad Fajloun. Evidence for in vitro antiophidian properties of aqueous buds extract of Eucalyptus against Montivipera bornmuelleri venom. Journal of Venom Research 2017, 8, 25 -30.

AMA Style

Joseph Khoury, Ranin Dabbousy, Riyad Sadek, Sayed Antoun, Walid Hleihel, Christian Legros, Ziad Fajloun. Evidence for in vitro antiophidian properties of aqueous buds extract of Eucalyptus against Montivipera bornmuelleri venom. Journal of Venom Research. 2017; 8 ():25-30.

Chicago/Turabian Style

Joseph Khoury; Ranin Dabbousy; Riyad Sadek; Sayed Antoun; Walid Hleihel; Christian Legros; Ziad Fajloun. 2017. "Evidence for in vitro antiophidian properties of aqueous buds extract of Eucalyptus against Montivipera bornmuelleri venom." Journal of Venom Research 8, no. : 25-30.

Full paper
Published: 30 August 2017 in European Journal of Organic Chemistry
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Various aromatic ketones were first functionalized next to the carbonyl function by deprotolithiation in the presence of a zinc salt followed by iodolysis. The outcome of the reactions was analyzed, and in particular their regioselectivity in the light of the calculated pKa values. Various halogenated ketones were next involved in copper‐catalyzed twofold C–N bond formation to obtain fused systems based on 2‐aminopyrimidines. Besides a potential antibacterial effect, 2‐aminobenzothiopyrano[4,3,2‐de]quinazoline was shown to inhibit PIM1 (IC50: 0.61 µm) and CDK2/cyclin A (IC50: 2.0 µm) kinases.

ACS Style

Madani Hedidi; Julien Maillard; William Erb; Frederic Lassagne; Yury S. Halauko; Oleg A. Ivashkevich; Vadim E. Matulis; Thierry Roisnel; Vincent Dorcet; Monzer Hamzé; Ziad Fajloun; Blandine Baratte; Sandrine Ruchaud; Stéphane Bach; Ghenia Bentabed-Ababsa; Florence Mongin. Fused Systems Based on 2-Aminopyrimidines: Synthesis Combining Deprotolithiation-in situ Zincation withN-Arylation Reactions and Biological Properties. European Journal of Organic Chemistry 2017, 2017, 5903 -5915.

AMA Style

Madani Hedidi, Julien Maillard, William Erb, Frederic Lassagne, Yury S. Halauko, Oleg A. Ivashkevich, Vadim E. Matulis, Thierry Roisnel, Vincent Dorcet, Monzer Hamzé, Ziad Fajloun, Blandine Baratte, Sandrine Ruchaud, Stéphane Bach, Ghenia Bentabed-Ababsa, Florence Mongin. Fused Systems Based on 2-Aminopyrimidines: Synthesis Combining Deprotolithiation-in situ Zincation withN-Arylation Reactions and Biological Properties. European Journal of Organic Chemistry. 2017; 2017 (39):5903-5915.

Chicago/Turabian Style

Madani Hedidi; Julien Maillard; William Erb; Frederic Lassagne; Yury S. Halauko; Oleg A. Ivashkevich; Vadim E. Matulis; Thierry Roisnel; Vincent Dorcet; Monzer Hamzé; Ziad Fajloun; Blandine Baratte; Sandrine Ruchaud; Stéphane Bach; Ghenia Bentabed-Ababsa; Florence Mongin. 2017. "Fused Systems Based on 2-Aminopyrimidines: Synthesis Combining Deprotolithiation-in situ Zincation withN-Arylation Reactions and Biological Properties." European Journal of Organic Chemistry 2017, no. 39: 5903-5915.