(1) Background: biological way is one of the most ecofriendly and safe strategies for nanomaterials synthesis. So, biosynthesis-green method was used for the preparation of Zn(II) complex (in the Nano scale) from the reaction of the schiff base ligand 2,2′-((1E,1′E)-(1,2-phenylenebis (azanylylidene)), bis(methanylylidene))bis(4-bromophenol), and Zn(II)sulphate. The biogenic ZnNP-T was characterized by different methods. Our purpose was to evaluate the ability of biosynthesis-green method for the preparation of Zn(II) complex as an antifungal agent against diseases from fungal species. (2) Methods: in this work, isolates of Pythium aphanidermatum and Trichderma longibrachiatum were obtained, and Trichderma longibrachiatum was used to prepare nano metal complex. We tested the pathogenicity of nano metal complex against seedling and germination of seeds, and we evaluated the effectiveness of ZnNP-T for growth promotion of Vicia feba in early stage and inhibitory activity against Pythium aphanidermatum. (3) Results: antagonistic activity of ZnNP-T was tested in vitro against Pythium aphanidermatum, and then the growth rates of Vicia faba were determined. The obtained data revealed that mycelial growth of pathogenic fungus was inhibited about 73.8% at 20 ppm. In addition, improved the total biomass of Vicia faba in the presence of P. aphanidermatum. All concentration of ZnNP-T positively affected root weight of Vicia faba seedlings, and positively affected shoot weight. Root and shoot lengths were affected by using 20 ppm of ZnNP-T with up to 180 and 96.5 mm of shoot and root length compared to that of the control, while germination percentage was significantly enhanced with up to 100% increase after 72 h of germination. (4) Conclusion: one of the modern challenges in vegetable or fruit production is to enhance seed germination and to grow healthy plants with strong root system. In future, there should be a focus on using of biogenic Zinc nano-complex as plant growth promoter agents.

The nano-metal complexes are found to be more impregnable than their parent drugs, therefore this study was aimed to synthesize Ag-nanocomplex of paracetamol (Para) by novel biosynthesis method. Paracetamol Ag-nanocomplex with the formula [Ag(Para)₂(NO₃)₂] has been characterized and synthesized on the basis of elemental analysis, thermal (TGA), conductivity, ¹H, ¹³C NMR and electronic spectral studies. Verticillium wilt, caused by Verticillium dahliae is considered one of the most serious diseases in olives. The use of Ag-nanocomplex as antifungal with different concentration (25, 50, 100 and 150 mg/ml) led to significant reductions of forming colonies of Verticillium wilt pathogenic fungi, with efficiencies reaching to 79.4, 82.9 and 81.8%, respectively at (100 mg/ml) against “V. dahliae, Fusarium acuminatum and Alternaria brassicae” LD₅₀ was also evaluated, while no changes in physical activity were detected, so this research opens outlooks for more study of fungal biogenic nanoparticles, indicating their use for controlling an agricultural pests

In this study, a convenient synthesis of a series of pyrimido[4,5-d]pyrimidine, pyrimidine acetohydrazide, and thieno[3,2-d]pyrimidine-7-carbohydrazide derivatives, via the reactions of versatile, readily accessible electrophilic and nucleophilic reagents with 2-thioxodihydropyrimidine-4,6(1H,5H)-dione 1, is described. Different biologically active moieties such as thiophene and pyridine are introduced in order to investigate their anticancer activity in vitro. In addition, a Schiff base ligand derived from the reaction of 5-bromosalicylaldehyde with 6-aminothieno[3,2-d]pyrimidine carbohydrazide 8 and its La(III) and Gd(III) complexes (in the bulk and at the nanoscale) were prepared and characterized by elemental analyses, IR spectra, magnetic measurement, and electronic absorption data. Chemical shifts of the different types of protons in the NMR spectra of the prepared Schiff base and its metal complexes are also reported. The nanocomplexes were characterized with a scanning electron microscope. The cytotoxicity of all newly synthesized compounds was demonstrated and they were tested for their activity against common pathogenic organisms and anticancer activity against human epithelial colorectal adenocarcinoma cells (Caco2). Some of the newly synthesized compounds had the best recorded anticancer activity against Caco2 cells. In addition, the three active compounds were tested for antibacterial activity. The three structures succeeded in affecting and stopping the growth of Klebsiella pneumoniae strain and the results revealed promising activity.