Adenanthera pavonina a perennial species of family Leguminosae is commonly known as red-bead tree. It is a medicinal plant traditionally used to treat various health conditions. The plant is reported to have a wide range of biological activities including astringent, anti-hemorrhagic, anti-diarrheal, anti-hematuria, anti-inflammatory, anti-rheumatic, anti-gout and anti-oxidant activities. Seeds are anticephalgic and also used for the treatment of paralysis and blood pressure. The Gas Chromatography Mass Spectroscopic (GCMS) and Gas Chromatographic Flame Ionization Detector (GCFID) spectra of plant oil indicated the presence of valuable constituents. The compounds identified by GCMS include 2-amylfuran, Pentanoic acid, Hexanoic acid, Decane, 2,4,Decadienal, C10 H18 O, Palmitic Acid, Octadec-9-enoic acid, and Stigmasta 3,5-diene-7-one.The unsaturated fatty acid content was found to be 44.628 % while unsaturated fatty acid were identified to be 2.53 % including other fatty acid as 52.502%. Main fatty acid identified were palmitic acid (60.999%), Linolenic acid (26.507%) Stearic acid (24.634%), Behenic acid (28.203%) and Decosahexonic acid (15.031%). The anti-oxidant activity and physicochemical analysis of oil including iodine value sap value was also performed to assess the bioactivity and stability characteristics of fixed oil. Iodine value was found to be 53.444 indicating low degree of saturation while Sap value was found out 283. The antioxidant potential of oil was found to be 77.187 %. Hence the current findings justified the traditional uses of this significant medicinal plant species.