This review includes results of scientific and clinical use of laser Doppler flowmetry (LDF) in patients with diabetes mellitus. LDF is a non-invasive method for the quantitative evaluation of microcirculation, which can assess microcirculatory rhythms and conduct functional tests with various impacts, allowing the exploration of regulatory mechanisms of microcirculation. LDF reveals specific diabetes changes in the regulatory function of microcirculation. Microcirculation disturbances, which are traditionally associated with the pathogenesis of complications, also occur in patients with early disorders of carbohydrate metabolism and may precede the manifestation of diabetes. However, this method is still not applied in clinical practice. In this review, we analysed factors limiting the implementation of LDF in practical medicine and suggest ways to improve its clinical significance.

In vivo laser-induced fluorescence spectroscopy (LIFS) is primarily used in oncology for the diagnosis of malignant tumors. This paper provides background for and describes experiments modeling nonmalignant local hypoxia and inflammation. LIFS techniques were used to assess the dynamics of induced fluorescence from endogenous porphyrins in the first case and the Photosens exogenous photosensitizer in the second case. In both cases, the fluorescence intensity was observed to be higher in the pathological area than in an intact area. This provides a strong impetus for taking a second look at the use of LIFS in oncology and also provides the foundation for a promising in vivo diagnosis method for ischemic hypoxia and inflammatory processes in areas other than oncology.

BACKGROUND: Laser Doppler flowmetry is widely used in scientific studies of blood microcirculation but constrained in clinical use due to the low diagnostic significance. OBJECTIVE: This research is aimed at creation of approaches to prognostic value

There are currently no effective measures to combat fibrosis in modern medical practice. One of the reasons for that is the late diagnosis associated with the lack of available clinical biomarkers and effective methods of non-invasive detection of the process. Fibrosis of the skin is characterized by fibrosis of the dermis, underlying tissues and is represented by a wide range of nosologies. Scleroderma and scars are of the greatest interest for the study. Skin changes in the development of bleomycin-induced fibrosis was studied in the experimental model using laser fluorescence spectroscopy and optical tissue oximetry. A significant increase in the rates of endogenous fluorescence of porphyrins, caused by inflammation and hypoxia, was detected at 7 and 21 days. An increased intensity of endogenous collagen fluorescence and a decreased specific oxygen uptake due to excess accumulation of the extracellular matrix were recorded on the 21st day after bleomycin treatment. Synchronous measurements of the collagen fluorescence and the specific oxygen uptake allowed to correlate the obtained data and the phases of the fibrogenic response described morphologically. The results allow to judge the severity of inflammation and hypoxia in the process of the fibrosis development. The objective and quantitative nature of the recorded parameters makes it possible to develop criteria for diagnosing the phases of fibrosis development.

Увеличивается ли тонус сосудов системы микроциркуляции при артериальной гипертонии?