Immunotherapy of bladder cancer is known to have favorable effects, although it is difficult to determine which patients will show a good response because of the different tumor microenvironments (TME). Here, we developed a bladder cancer-on-a-chip (BCOC) to mimic the TME using three-dimensional (3D) bioprinting and microfluidic technology. We fabricated a T24 and a 5637-cell line-based BCOC that also incorporated MRC-5, HUVEC, and THP-1 cells. We evaluated the effects of TME and assessed the immunologic reactions in response to different concentrations of Bacillus Calmette–Guérin (BCG) via live/dead assay and THP-1 monocytic migration, and concentrations of growth factors and cytokines. The results show that cell viability was maintained at 15% filling density in circle-shaped cell constructs at 20 μL/min microfluidic flow rate. A 3D co-culture increased the proliferation of BCOCs. We found that the appropriate time to evaluate the viability of BCOC, concentration of cytokines, and migration of monocytes was 6 h, 24 h, and three days after BGC treatment. Lastly, the immunotherapeutic effects of BCOC increased according to BCG dosage. To predict effects of immunotherapeutic agent in bladder cancer, we constructed a 3D bioprinted BCOC model. The BCOC was validated with BCG, which has been proven to be effective in the immunotherapy of bladder cancer.

In vivo cells express their characteristics in three-dimensional (3D) microenvironments via cell-cell interactions through autocrine, contact-dependent, paracrine, and synaptic signaling, often between heterologous cell types. Various in vitro 3D microwell-based culture methods have been proposed to further identify cellular characteristics by recreating cellular environments, typically in the form of spheroids and organoids, thereby realizing contact-based cell-cell interactions. However, in vivo cells generally exhibit multiple cellular interaction modes that have not been completely evaluated using existing microwell-based methods. This has led to a demand for more advanced and comprehensive methods. This study introduces a novel apparatus, the membrane-bottomed microwell (MBM) for non-contact co-cultures and 3D cell cultures. The MBM is a combination of a Transwell and a microwell array; these have previously been utilized to facilitate heterologous cell co-culturing and spheroid 3D cell culturing, respectively. In the Transwell insert, the lower part of the MBM is immersed in the culture media in which the cells are being two-dimensionally (2D) cultured, and the spheroids of the MBM are affected by the 2D cultured cells via the membrane at the bottom of the microwell. Here, we describe the methods for manufacturing the MBM in detail and elucidate the results of simulations of diffusion through the bottom of the membrane. We validate the proposed MBM for the spheroid culture of spermatogonial stem cells (SSCs), which had previously been 2D co-cultured with Sandos inbred mouse (SIM)-derived 6-thioguanine- and ouabain-resistant (STO; a mouse embryonic feeder cell line) feeder cells. The proposed system is shown to facilitate successful SSC spheroid culturing with paracrine signaling of STOs through an apparatus that simplifies both the loading and the evaluation processes; therefore, we believe that our findings will enable a more comprehensive understanding of SSCs and associated phenomena and that our system can be applied to various in vitro cell and tissue experiments.

Over the past few decades, research on life in space has increased. Owing to the expensive nature of and the challenges associated with conducting experiments in real space, clinostats, which continuously randomize the gravity vector by using motors, have been used to generate simulated microgravity (SMG) on Earth. Herein, by using a 3D printing method, we develop a customized small-sized clinostat (CS clinostat) that is easy to manufacture, inexpensive, and robust. Moreover, we develop and fabricate a gas-permeable polydimethylsiloxane culture dish that fits inside the CS clinostat. To validate SMG generation, ovarian cancer cells (OV- 90, TOV-21G, and Caov-3) were applied to demonstrate a significant reduction in caveolin-1 expression, a biomarker of SMG, indicating SMG generation. The proposed CS clinostat system has good accessibility for SMG research, which makes it useful as a tool for biologists, who are unfamiliar with conventional clinostat equipment, to conduct preliminary studies in the space environment.

Solar updraft towers (SUTs) are used for renewable power generation, taking advantage of the thermal updraft air flow caused by solar energy. Aerodynamic devices have been applied to SUTs to improve their performance and the baffle is one such device. Here, we investigate the effect of baffle installation on the thermo-fluid dynamic phenomena in the collector of an SUT and how it enhances the overall SUT performance using computational fluid dynamics analysis. Two geometric parameters (height and width of baffle) and two thermal boundary conditions of the baffle (adiabatic condition and heat flux condition) were tested through simulations with 10 different models. The vortex generated by the baffle has a positive effect on the delivery of heat energy from the ground to the main flow; however, one disadvantage is that the baffle inherently increases the resistance of the main flow. Over 3% higher kinetic power was achieved with some of the simulated baffle models. Therefore, an optimum design for baffle installation can be achieved by considering the positive and negative thermo-fluid dynamics of baffles.

The solar updraft tower (SUT) is a renewable power generation system that uses the natural convection phenomenon of the ground’s air heated by solar radiation. The baffle is a thermo-fluid dynamic structure that improves the heat exchange efficiency and has been recently reported to be a useful tool to increase the output of the SUT. However, one of the less well-known issues is the relationship between the thermo-fluid dynamic characteristics of the flow in the collector of the SUT and the installation location of the baffle and how this affects the power output and SUT efficiency. In this study, the positive and negative thermo-fluid dynamic effects of the baffle, which vary depending on the installation location, are quantitatively analyzed, and the best location is predicted where the overall kinetic power generated by the SUT is maximized. The target SUT model consists of a chimney (12 m height and 0.25 m diameter) and a collector (1 m height and 10 m diameter), and a total of eight model cases are calculated. The results confirm that the kinetic power is lower or higher than that of the control model having no baffle, depending on the baffle installation location. When the position of the baffle is 4.5 m from the center, the increase in kinetic power is maximized by 8.43%. Two important conclusions are that the baffle should interfere minimally with the progress of the main flow into the chimney, generating kinetic power, and at the same time, the baffle should isolate the inner recirculating flow in order to accumulate the heat in the collector so that the natural convection strength is maximized. The perspective gained from the resulting data is useful for SUT design and for pursuing a higher efficiency in the future.