Polydimethylsiloxane (PDMS) membranes are used in various applications, such as microvalves, micropumps, microlenses, and cell culture substrates, with various thicknesses from microscale to nanoscale. In this study, we propose a simple fabrication method for PDMS membranes on a water surface, referred to as the floating-on-water (FoW) method. FoW can be used to easily fabricate PDMS membranes with thicknesses of a few micrometers (minimum 3 μm) without special equipment. In addition, as the membrane is fabricated on the water surface, it can be easily handled without damage. In addition, alternative membrane structures were demonstrated, such as membrane-on-pins and droplet-shaped membranes. FoW can be widely used in various applications that require PDMS membranes with microscale thicknesses.

Over the past few decades, research on life in space has increased. Owing to the expensive nature of and the challenges associated with conducting experiments in real space, clinostats, which continuously randomize the gravity vector by using motors, have been used to generate simulated microgravity (SMG) on Earth. Herein, by using a 3D printing method, we develop a customized small-sized clinostat (CS clinostat) that is easy to manufacture, inexpensive, and robust. Moreover, we develop and fabricate a gas-permeable polydimethylsiloxane culture dish that fits inside the CS clinostat. To validate SMG generation, ovarian cancer cells (OV- 90, TOV-21G, and Caov-3) were applied to demonstrate a significant reduction in caveolin-1 expression, a biomarker of SMG, indicating SMG generation. The proposed CS clinostat system has good accessibility for SMG research, which makes it useful as a tool for biologists, who are unfamiliar with conventional clinostat equipment, to conduct preliminary studies in the space environment.

Microwells are used in studies to mimic the in vivo environment through an in vitro environment by generating three-dimensional cell spheroids. These microwells have been fabricated in various shapes using different methods according to the research purpose. However, because all microwells up to now have an open top, it has been difficult to culture spheroids of floating cells due to their low density, such as human adipose-derived stem cells (hASCs) that differentiate into adipocytes. Therefore, the labor-intensive hanging droplet method has been mainly used for the study of adipocytes. Here, we introduce a sigma-well, which is a microwell in the shape of the Greek letter sigma (σ) with a roof. Because of its unique shape, the sigma-well is advantageous for the culture of floating cells by reducing cell loss and external interference. The sigma-well was fabricated using the principle of surface tension of polydimethylsiloxane as well as air trapping and thermal expansion. Unlike conventional microwells, because the center of the bottom surface and the inlet of the sigma-well are not located on the same line and have a difference of approximately 218 μm, the spheroids are cultured more stably and may not escape the cavity. In this study, hASC and adipocyte spheroids differentiated using these sigma-wells were successfully cultured. In addition, through cytokine diffusion simulation, it was confirmed that the diffusion and mass transfer in the sigma-well was lower than that in the conventional microwell. It is expected that the morphological features of the sigma-well, which cannot be easily obtained by other methods, can be beneficial for the study of buoyant cell types such as adipocytes.

Microfluidic devices have emerged as powerful tools for cell-based experiments, offering a controlled microenvironment that mimic the conditions within the body. Numerous cell experiment studies have successfully utilized microfluidic channels to achieve various new scientific discoveries. However, it has been often overlooked that undesired and unnoticed propagation of cellular molecules in such bio-microfluidic channel systems can have a negative impact on the experimental results. Thus, more careful designing is required to minimize such unwanted issues through deeper understanding and careful control of chemically and physically predominant factors at the microscopic scale. In this paper, we introduce a new approach to improve microfluidic channel design, specifically targeting the mitigation of the aforementioned challenges. To minimize the occurrence of undesired cell positioning upstream from the main test section where a concentration gradient field locates, an additional narrow port structure was devised between the microfluidic upstream channel and each inlet reservoir. This port also functioned as a passive lock that hold the flow at rest via fluid-air surface tension, which facilitated manual movement of the device even when cell attachment was not achieved completely. To demonstrate the practicability of the system, we conducted experiments and diffusion simulations on the effect of endocrine disruptors on germ cells. To this end, a bisphenol-A (BPA) concentration gradient was generated in the main channel of the system at BPA concentrations ranging from 120.8 μM to 79.3 μM, and the proliferation of GC-1 cells in the BPA gradient environment was quantitatively evaluated. The features and concepts of the introduced design is to minimize unexpected and ignored error sources, which will be one of the issues to be considered in the development of microfluidic systems to explore extremely delicate cellular phenomena.

The rim seal of the gas turbine is intended to protect the material of the turbine disk from hot combustion gases. The study of the rim seal structure is important to minimise the coolant flow and maximise the sealing effect. In this paper, a wave-shaped rim seal for stator disks is proposed and its effect is confirmed by numerical analysis. To characterise the flow phenomena near the wave-shaped rim seal, a simplified model of the wave-shaped rim seal (Type 1 model), which excludes the rotor blade and stator vane, is analysed and compared with the conventional rim seal. Then, through analysis of the wave-shaped rim seal geometry (Type 2 model), which includes the rotor blade and stator vane, a reduction in egress and ingress flow was observed owing to the wave-shaped rim seal, and the sealing effectiveness on the stator disk of turbine was increased by up to 3.8%. Implementation of the wave-shape geometry in the radial seal is a novel choice for turbine designers to consider in future for better-performing and more-efficient turbines.

In Korea, as power facilities are aging like overseas nations, maintenance and replacement costs of power facilities are expected to increase rapidly. In order to operate power facilities with a limited budget efficiently, it is necessary to introduce Asset Management System (AMS) in maintenance of the power facility in line with the global trend. To this end, it is essential for power companies to predict the remaining lifetime of power facilities and develop Probability of Failure (PoF) in their own way depending on the type and operating conditions of power facilities. Existing studies only calculated the statistical life expectancy of the power facility, but in this study, both of the statistical life expectancy and the condition remaining lifetime are presented in order to assess the PoF of power facility, in which the statistical life expectancy is calculated by using Weibull distributions. In this study, we present the criteria for determining the condition remaining lifetime using Health Index, inspection and diagnosis data of the pad mounted transformer operated by Korea Electric Power Corporation (KEPCO) in the field. In addition, the final remaining lifetime of the pad mounted transformer was derived through the folding function and was converted into PoF using Hazard rate distribution based on the apparent age. PoF derived in this study is expected to be used in the risk matrix of the AMS and useful in establishing the systematic maintenance plan of the pad mounted transformer.