Potato is an economically important vegetable crop in Egypt. Weed infestation, especially broad-leafed, during the vegetative growth stage substantially affects both crop yield and tuber quality. In the current study, the impact of new ready-mix pre-emergent herbicides on broadleaf weeds, tuber yield, and quality was evaluated. The two-year field experiment comprised the following treatments: (1) Un-weeded control, (2) Hand hoeing, (3) Sencor, (4) Ecopart, (5) Zeus, (6) Kroki, and (7) Flomex. The results showed that weed control treatments significantly reduced the weed density compared to un-weeded control and the herbicides efficacy reached over 90%. The herbicidal treatments also significantly increased the activity of antioxidant enzymes peroxidases (POX) and catalase (CAT) and improved the non-enzymatic antioxidant (carotenoids) compared to un-weeded control. Conversely, the higher content of malondialdehyde (MDA) in potato leaves was obtained for un-weeded control. Moreover, weed control treatments caused significant enhancement in plant growth parameters, yield, and its components in addition to tuber quality of potato. Compared to the un-weeded control, maximum tuber yield was observed in Flomex followed by Ecopart, Kroki, Zeus, and Sencor, respectively. The higher number of tubers and total yield were recorded in plants treated with Flomex plus compared to all the other treatments. Higher content of total soluble sugar, total soluble protein, and total starch content was observed in weed control treatments compared with un-weeded control. Based on Pearson’s correlation and heatmap analysis, the changes in agro-physiological parameters data are linked to the herbicidal treatments. The results indicate that the applied herbicides could be alternative products for Sencor and an option for controlling broadleaved weeds. However, further studies are needed to ensure their efficacy and safety under other conditions.

Biochar application is a promising sustainable strategy for enhancing soil properties thus crop production. However, biochar application to soil certainly alters its biological and physical properties, and could require extra costs. Therefore, biochar suitability to agroecosystems must be proactively estimated. The advantage of biochar addition to poor fertile or weathered soils has been well studies, however, its feasibility to fertile soil under low quality (saline) irrigation water was not frequently studied. Consequently, this work investigates the hypothesis of whether the application of biochar at a rate of 4.8 tons/ha to fertile soil (Nile Valley, Giza, Egypt) would ameliorate the negative effects of saline irrigation regime (3000 ppm) on tomato crop and soil. The results of two seasons experiments showed that saline irrigation significantly reduced tomato crop yield by an average reduction ratio of 51%, and biochar addition could not compensate such reduction. Furthermore, biochar did not reduce accumulated Na⁺ in fruits or roots. Tomato fruits produced from biochar-added soil were lower in TSS levels (41.7% reduction ratio) yet larger in diameter by approximately 1.5-fold increase. Interestingly, biochar addition into soil greatly promotes the length of stem-borne lateral roots and elevates the expression of LeNR (encodes nitrate reductase enzyme) in leaves yet under fresh irrigation regime. For soil properties, biochar application enhanced the soil properties under either saline or fresh water irrigation conditions. Collectively, it is assumed that biochar application to fertile soil in Nile Valley of Egypt could not alleviate tomato fruits yield reduction affected by applied saline irrigation regime.

The isolation of high-quality RNA from strawberry leaves and fruits is notoriously cumbersome. Both tissues are extremely rich in active secondary metabolites, as phenolics and pigments that inevitably perturb the isolation of RNA. Many protocols have been developed to address this problem. However, they are either costly, or time-consuming, in particular for high number of many plant samples. We describe here a new method with an easy-to-handle approach to obtain high-quality RNA from strawberry leaves and fruits. The method, referred to as double lysis, uses a novel combination of CTAB and Trizol protocols. Compared to conventional Trizol-dependent protocols, either used individually, or in a commercial spin-column kit, the new method yields RNA at lower costs, but of significantly improved quality. The RNA obtained by double lysis was very pure as indicated by 260/280 ratios of 2.06 (leaves) and 2.07 (fruits), while 260/230 ratio was 2.07 and 1.75, respectively. Also visually, RNA sediments from double lysis showed a white color, indicative of efficient elimination of polyphenolics and pigments. In contrast, traditional Trizol method produced reddish precipitates. The purity of RNA isolated by double lysis enabled successful removal of genomic DNA and, thus, allowed for more efficient cDNA synthesis and RT-PCR. In addition, the suggested method is able to yield RNA with fully preserved integrity from strawberry leaves, fruits in addition to petals and roots. Double lysis is a new RNA isolation protocol developed through the integrative coupling of CTAB and Trizol reagents. The method is cost-effective, robust, time-saving, and can cope even with recalcitrant plant tissues with high contents of phenolics and pigments, such as strawberry leaves and fruits.

The severity of Botrytis cinerea growth could be significantly inhibited in mature strawberry fruits by priming fungal resistance in harvested berries after the proactive application of UV-B light (312 nm). Harvested fruits were irradiated by accumulative UV-B light amounts (0, 0.33, 0.65, and 1.3 mJoule/cm2) before being artificially infected with B. cinerea. PCR assay could estimate a reduction ratio of 50 % in fungal DNA in fruits proactively exposed to UV-B light (1.3 mJoule/cm2). These fruits showed a minimum fungal severity level compared to non-UV-B treated fruits (0 mJoule/cm2), which were harshly rotted with velvety gray mold growth. To reveal a side of possible molecular mechanisms behind UV-B induced immunity in ripe berries against B. cinerea, the expression profiles of some resistance-related genes were quantified using real-time PCR on day 1 and day 2 after infection. UV-B significantly sharpens the expression of strawberry defense gene FaBG2-1 which encodes the fungal cell wall degrading enzyme β-1-3-glucanase. Furthermore, jasmonic acid (JA) biosynthesis key gene FaAOS was strongly up-regulated after exposure to UV-B in infected or non-infected fruits on day 2. Equally important, the expression of FaNES1, volatile terpenoids linalool/nerolidol synthase gene, was moderately elevated. ABA signaling gene FaPYR1 was found to be more responsive on day 2 after UV-B irradiation. Collectively, we hypothesize that UV-B proactive irradiation can encourage the enhancement of the molecular immune system in mature strawberry fruits against possible B. cinerea infection postharvest. Further studies are needed to reveal UV-B potential as a sustainable priming agent against plant diseases.