This study aimed to investigate the potential adverse effects of the practical application of copper sulfate on yellow catfish (Pelteobagrus fulvidraco) and to provide insights into the gill toxicity induced by copper sulphate. Yellow catfish were exposed to a conventional anthelmintic concentration of copper sulphate (0.7 mg/L) for seven days. Oxidative stress biomarkers, transcriptome, and external microbiota of gills were examined using enzymatic assays, RNA-sequencing, and 16S rDNA analysis, respectively. Copper sulphate exposure led to oxidative stress and immunosuppression in the gills, with increased levels of oxidative stress biomarkers and altered expression of immune-related differentially expressed genes (DEGs), such as IL-1β, IL4Rα, and CCL24. Key pathways involved in the response included cytokine–cytokine receptor interaction, NOD-like receptor signaling pathway, and Toll-like receptor signaling pathway. The 16S rDNA analysis revealed copper sulphate altered the diversity and composition of gill microbiota, as evidenced by a significant decrease in the abundance of Bacteroidotas and Bdellovibrionota and a significant increase in the abundance of Proteobacteria. Notably, a substantial 8.5-fold increase in the abundance of Plesiomonas was also observed at the genus level. Our findings demonstrated that copper sulphate induced oxidative stress, immunosuppression, and gill microflora dysbiosis in yellow catfish. These findings highlight the need for sustainable management practices and alternative therapeutic strategies in the aquaculture industry to mitigate the adverse effects of copper sulphate on fish and other aquatic organisms.

Aeromonas hydrophila is an opportunistic pathogen responsible for a number of diseases in freshwater farming. Moreover, the bacterium has been identified as a zoonotic pathogen that threatens human health. Antibiotics are widely used for treatments of infectious diseases in aquaculture. However, the abuse of antibiotics has led to the emergence of antimicrobial resistant strains. Thus, novel strategies are required against resistant A. hydrophila strains. The quorum sensing (QS) system, involved in virulence factor production and biofilm formation, is a promising target in identifying novel drugs against A. hydrophila infections. In this study, we found that thymol, at sub-inhibitory concentrations, could significantly reduce the production of aerolysin and biofilm formation by inhibiting the transcription of genes aerA, ahyI, and ahyR. These results indicate that thymol inhibits the quorum sensing system. The protective effects of thymol against A. hydrophila mediated cell injury were determined by live/dead assay and lactate dehydrogenase (LDH) release assay. Moreover, the in vivo study showed that thymol could significantly decrease the mortality of channel catfish infected with A. hydrophila. Taken together, these findings demonstrate that thymol could be chosen as a phytotherapeutic candidate for inhibiting quorum sensing system-mediated aerolysin production and biofilm formation in A. hydrophila.

We developed a method for determination of imidacloprid and its metabolites 5-hydroxy imidacloprid, olefin imidacloprid, imidacloprid urea and 6-chloronicotinic acid in Procambarus clarkii (crayfish) tissues using quick, easy, cheap, effective, rugged, and safe (QuEChERS) and high-performance liquid chromatography-triple quadrupole mass spectrometry. Samples (plasma, cephalothorax, hepatopancrea, gill, intestine, and muscle) were extracted with acetonitrile containing 0.1% acetic acid and cleaned up using a neutral alumina column containing a primary secondary amine. The prepared samples were separated using reverse phase chromatography and scanned in the positive and negative ion multiple reaction-monitoring modes. Under the optimum experimental conditions, spiked recoveries for these compounds in P. clarkii samples ranged from 80.6 to 112.7% with relative standard deviations of 4.2 to 12.6%. The limits of detection were 0.02–0.5 μg·L⁻¹, the limits of quantification were 0.05–2.0 μg·L⁻¹ and the method of quantification was 0.05–2.0 μg·kg⁻¹. The method is rapid, simple, sensitive and suitable for rapid determination and analysis of imidacloprid and its metabolites in P. clarkii tissues.

Monogeneans are a serious threat to the development of aquaculture due to the severe economic losses they cause. The prevention and treatment of this disease are increasingly difficult because of the environmental and health concerns caused by the use of chemical anthelmintics and the emergence of drug resistance. It is thus necessary to search for effective alternatives for the treatment of monogenean infections. In the current study, anthelmintic efficacy of 16 selected essential oils (EOs) was investigated using the goldfish (Carassius auratus)–Gyrodactylus kobayashii model. The screening experiment indicated that palmarosa oil and curcuma oil had satisfactory anthelmintic activity against G. kobayashii with EC₁₀₀ values of 10 and 12 mg/L after 24-h exposure, respectively. The in vivo and in vitro assays indicated anthelmintic efficacy of palmarosa oil against G. kobayashii was in a time and dose-dependent manner. Interestingly, curcuma oil showed an anesthetic effect on G. kobayashii, and its anthelmintic activity was dose-dependent rather than time-dependent in the concentration range tested in this study. Additionally, the 24-h LC₅₀ (50% lethal concentration) against goldfish of these two EOs was 8.19-fold and 5.54-fold higher than their corresponding EC₅₀ (50% effective concentration) against G. kobayashii, respectively. Moreover, exposure to these two EOs at 100% effective concentration against G. kobayashii had no serious physiological and histopathological influence on goldfish. These results demonstrated a high safety for goldfish of these two EOs. Overall, palmarosa oil and curcuma oil could be potential candidates for the treatment of G. kobayashii infections in aquaculture.

Aeromonas hydrophila, an opportunistic aquatic pathogen widely spread in aquatic environments, is responsible for a number of infectious diseases in freshwater aquaculture. In addition, A. hydrophila can transmit from diseased fish to humans and results in health problems. The occurrence of antibiotic-resistant bacterial strains restricts the application of antibiotics and is responsible for failure of the treatment. Moreover, residues of antibiotics in aquatic products often threaten the quality and safety. Therefore, alternative strategies are called to deal with infections caused by antibiotic-resistant bacteria. Aerolysin, one of the most important virulence factors of A. hydrophila, is adopted as a unique anti-virulence target on the basis of the anti-virulence strategy to battling infections caused by A. hydrophila. Palmatine, an isoquinoline alkaloid from a variety of herbal medicines that showed no anti-A. hydrophila activity, could reduce hemolysis of the bacterium by decreasing aerolysin production. The results of the qPCR assay demonstrated that the transcription of the aerA gene was suppressed. Moreover, cell viability and in vivo study showed that palmatine treatment could decrease the pathogenicity of A. hydrophila both in vitro and in vivo. In summary, palmatine is a leading compound against A. hydrophila-associated infection in aquaculture by inhibiting the expression of aerolysin.

Channel catfish (Ictalurus punctatus) is a widely consumed freshwater fish known for its nutritional value but is highly prone to spoilage. This study investigated the quality changes of catfish muscle tissue under refrigeration and freezing through physicochemical, metabolomic, and microbial analyses. Results revealed that sensory scores decreased significantly during storage, with frozen samples maintaining similar scores to refrigerated ones after extended periods. Protein degradation and lipid oxidation, indicated by TVB-N and TBARS levels, were more pronounced during prolonged freezing. Metabolomic profiling identified 261 differential metabolites under long-term freezing, including elevated phosphatidylcholines, sphingomyelins, and disrupted amino acid pathways. Shifts in spoilage-associated microbial genera, such as Pseudomonas, and the correlations between microbial genera and specific metabolites, such as Methylobacterium with methylmalonic acid, highlighted microbial-driven spoilage processes. These findings provided a comprehensive understanding of quality deterioration during storage, guiding the development of enhanced preservation strategies for aquatic products.