Vineyard-living microbiota affect grapevine health and adaptation to changing environments and determine the biological quality of soils that strongly influence wine quality. However, their abundance and interactions may be affected by vineyard management. The present study was conducted to assess whether the vineyard soil microbiome was altered by the use of biostimulants (arbuscular mycorrhizal fungi (AMF) inoculation vs. non-inoculated) and/or irrigation management (fully irrigated vs. half irrigated). Bacterial and fungal communities in vineyard soils were shaped by both time course and soil management (i.e., the use of biostimulants and irrigation). Regarding alpha diversity, fungal communities were more responsive to treatments, whereas changes in beta diversity were mainly recorded in the bacterial communities. Edaphic factors rarely influence bacterial and fungal communities. Microbial network analyses suggested that the bacterial associations were weaker than the fungal ones under half irrigation and that the inoculation with AMF led to the increase in positive associations between vineyard-soil-living microbes. Altogether, the results highlight the need for more studies on the effect of management practices, especially the addition of AMF on cropping systems, to fully understand the factors that drive their variability, strengthen beneficial microbial networks, and achieve better soil quality, which will improve crop performance.

Proximal sensing is being integrated into vineyard management as it provides rapid assessments of spatial variability of soils’ and plants’ features. The electromagnetic induction (EMI) technology is used to measure soil apparent electrical conductivity (EC_a) with proximal sensing and enables to appraise soil characteristics and their possible effects on plant physiological responses. This study was conducted in a micro irrigated Cabernet Sauvignon (Vitis viniferaL.) vineyard to investigate the technical feasibility of appraising plant water status and its spatial variability using soil EC_aand must carbon isotope ratio analysis (δ¹³C). Soil temperature and soil water content were monitoredin-situusing time domain reflectometry (TDR) sensors. Soil EC_awas measured with EMI at two depths [0–1.5 m (deep EC_a) and 0–0.75 m (shallow EC_a)] over the course of the crop season to capture the temporal dynamics and changes. At the study site, the main physical and chemical soil characteristics, i.e. soil texture, gravel, pore water electrical conductivity (EC_e), organic carbon, and soil water content at field capacity, were determined from samples collected auguring the soil at equidistant points that were identified using a regular grid. Midday stem water potential (Ψ_stem) and leaf gas exchange, including stomatal conductance (g_s), net carbon assimilation (A_n), and intrinsic water use efficiency (WUE_i) were measured periodically in the vineyard. The δ¹³C of produced musts was measured at harvest. The results indicated that soil water content (relative importance = 24%) and texture (silt: relative importance = 22.4% and clay: relative importance = 18.2%) were contributing the most towards soil EC_a. Deep soil EC_awas directly related to Ψ_stem(r² = 0.7214) andg_s(r² = 0.5007). Likewise, δ¹³C of must was directly related to Ψ_stem(r² = 0.9127),g_s(r² = 0.6985), andA_n(r² = 0.5693). Results from this work provided relevant information on the possibility of using spatial soil EC_asensing and δ¹³C analysis to infer plant water status and leaf gas exchange in micro irrigated vineyards.

Grapevine productivity, and berry and wine flavonoid concentration, depend on the interactions of cultivar, environment, and applied cultural practices. We characterized the effects that mechanical leaf removal and irrigation treatments had on the flavonoid concentration of ‘Merlot’ (Vitis vinifera, L.) grape berries and wines in a hot climate over two growing seasons with contrasting precipitation patterns. Leaves were removed by machine, either at prebloom (PBLR), or at post-fruit-set (PFLR), or not removed (control) and irrigation was either applied as sustained deficit irrigation (SDI) at 0.8 of crop evapotranspiration (ET_c) from budbreak to fruit set, or regulated deficit irrigation (RDI) at 0.8 ET_c from bud break to fruit set, 0.5 ET_c from fruit set to veraison, and 0.8 ET_c from veraison to harvest, of ET_c In 2014, PFLR reduced the leaf area index (LAI) compared to control. The RDI decreased season-long leaf water potential (Ψ_Int) compared to SDI. However, in 2015, none of the treatments affected LAI or Ψ_Int. In 2014, berry flavonoid concentrations were reduced by PBLR as well as SDI. SDI increased the flavonoid concentrations in wine, and PFLR increased some wine flavonols in one season. No factor affected the concentrations of wine proanthocyanidins or mean degree of polymerization. Thus, mechanical PFLR and RDI may increase berry flavonoid accumulation without yield reduction, in red wine grapes cultivars grown in hot climates when precipitation after bud break is lacking. However, spring precipitation may influence the effectiveness of these practices as evidenced by this work in a changing climate.

The spatial variability in vineyard soil might negatively affect wine composition, leading to inhomogeneous flavonoid composition and aromatic profiles. In this study, we investigated the spatial variability in wine chemical composition in a Cabernet Sauvignon (Vitis vinifera L.) vineyard in 2019 and 2020. Because of the tight relationships with soil profiles, mid-day stem water potential integrals (Ψ_stem Int) were used to delineate the vineyard into two zones, including Zone 1 with relatively higher water stress and Zone 2 with relatively lower water stress. Wine from Zone 2 generally had more anthocyanins in 2019. In 2020, Zone 1 had more anthocyanins and flavonols. Zone 2 had more proanthocyanidin extension and terminal subunits as well as total proanthocyanidins in 2020. According to the Principal Component Analyses (PCA) for berry and wine chemical composition, the two zones were significantly different in the studied wine aromatic compounds. In conclusion, this study provides evidence of the possibility of managing the spatial variability of both wine flavonoid composition and aromatic profiles through connecting vineyard soil variability to grapevine season-long water status.