Manganese (Mn) is an essential element that intervenes in several plant metabolic processes. The olive tree, and its fruits and leaves, are known as a source of nutraceuticals since they are rich in biophenols. However, there is still a serious lack of data about biophenolic distribution in olive stems and roots under Mn fertilisation. In this context, our study aimed to examine the effects of Mn fertilisation on the biophenolic profile in the leaves, stems, and roots of the ‘Istarska bjelica’ olive cultivar. The experiment was set up in a greenhouse, during a period of five months, as a random block design consisting of three treatments with varying Mn concentrations in full-strength Hoagland’s nutrient solution (0.2 µM Mn, 12 µM Mn, and 24 µM Mn). The obtained results indicate that the amount of Mn in the examined olive plant tissues was significantly higher under 12 µM Mn and 24 µM Mn treatments compared to that of the 0.2 µM Mn treatment. While the concentration of biophenols varied in roots depending on the compound in question, a strong positive impact of the increased Mn concentration in nutrient solution (12 µM Mn and 24 µM Mn) on the concentrations of the main biophenolic compounds was observed in stems. The concentration of oleuropein in leaves almost doubled at 24 µM Mn, with the highest Mn concentration, as compared to the 0.2 µM Mn treatment. The obtained results led to the conclusion that the supply of Mn could enhance the concentration of some biologically active compounds in olives grown hydroponically, implying a critical need for further investigation of Mn fertilisation practices in the conventional olive farming system.

Nitrate leaching through soil layers to groundwater may cause significant degradation of natural resources. The aims of this study were: (i) to estimate soil hydraulic properties (SHPs) of the similar soil type with same management on various locations; (ii) to determine annual water dynamics; and (iii) to estimate the impact of subsoil horizon properties on nitrate leaching. The final goal was to compare the influence of different SHPs and layering on water dynamics and nitrate leaching. The study was conducted in central Croatia (Zagreb), at four locations on Calcaric Phaeozem, Calcaric Regosol, and Calcaric Fluvic Phaeozem soil types. Soil hydraulic parameters were estimated using the HYPROP system and HYPROP-FIT software. Water dynamics and nitrate leaching were evaluated using HYDRUS 2D/3D during a period of 365 days. The amount of water in the soil under saturated conditions varied from 0.422 to 0.535 cm³ cm⁻³ while the hydraulic conductivity varied from 3 cm day⁻¹ to 990.9 cm day⁻¹. Even though all locations have the same land use and climatic conditions with similar physical properties, hydraulic parameters varied substantially. The amount and velocity of transported nitrate (HYDRUS 2D/3D) were affected by reduced hydraulic conductivity of the subsoil as nitrates are primarily transported via advective flux. Despite the large differences in SHPs of the topsoil layers, the deeper soil layers, having similar SHPs, imposed a buffering effect preventing faster nitrate downward transport. This contributed to a very similar distribution of nitrates through the soil profile at the end of simulation period. This case study indicated the importance of carefully selecting relevant parameters in multilayered soil systems when evaluating groundwater pollution risk.

Erosion has been reported as one of the top degradation processes that negatively affect agricultural soils. The study objective was to identify hydropedological factors controlling soil water dynamics in erosion-affected hillslope vineyard soils. The hydropedological study was conducted at identically-managed Jastrebarsko (location I), and Jazbina (II) and (III) sites with Stagnosol soils. Soil Hydraulic Properties (SHP) were estimated on intact soil cores using Evaporation and WP4C methods; soil hydraulic functions were fitted using HYPROP-FIT software. For Apg and Bg/Btg horizons, uni- and bimodal soil hydraulic models could be well fitted to data; although, the bimodal model performed better in particular cases where data indicated non-uniform pore size distribution. With these SHP estimations, a one-year (2020) water flow scenario was simulated using HYDRUS-1D to compare water balance results obtained with uni- and bimodal hydraulic functions. Simulation results revealed relatively similar flux distribution at each hillslope position between the water balance components infiltration, surface runoff, and drainage. However, at the bottom profile at Jastrebarsko, bimodality of the hydraulic functions led to increased drainage. Soil water storage was reduced, and the vertical movement increased due to modified soil water retention curve shapes. Adequate parameterization of SHP is required to capture the hydropedological response of heterogenous erosion-affected soil systems.

Echinochloa crus-galli (L.) P. Beauv. is the most common monocotyledonous weed in maize crops in Croatia. Crop–weed interference is influenced by weed emergence patterns, and knowledge of the timing of weed emergence is crucial for the development of an efficient integrated weed-management program. Therefore, two-year field experiments were conducted in a maize crop sown in early May in continental Croatia to determine the emergence pattern of E. crus-galli from natural seedbank. In laboratory studies, the estimated base temperature and base water potential for the Croatian ecotype of E. crus-galli were 10.8 °C and −0.97 MPa, respectively. Then, the estimated germination parameters were compared with the values embedded in the AlertInf model from Italy (Veneto) to calibrate this hydrothermal model. The estimated hydrothermal units were around 28 for the onset (10%) and 93 for the middle (50%) emergence of E. crus-galli. Our findings showed that the AlertInf model satisfactorily simulated the emergence of E. crus-galli in maize crop in Croatia (EF = 0.97 in 2019 and 0.98 in 2020), indicating its potential use in other geographical areas

Soil bulk density (BD) is often used to assess soil properties related to soil structure and general soil quality, or to convert weight units to volume/area units. Because collecting undisturbed soil samples (cores) and directly measuring BD is laborious and time-consuming, data on BD are often lacking. Pedotransfer functions (PTFs) can be used to predict BD from more readily available (easily measured) properties. However, specific PTFs for specific range of soils should be developed. In this study, soil organic matter (SOM) and clay contents of 90 forest Pseudogleys horizons, distributed across 11 locations in continental Croatia, were used to develop a multiple linear regression equation that predicts BD. The results have shown that the increase in BD due to the unit increase in clay content is lower than the decrease in BD due to the unit decrease in SOM content. PTF performance was relatively high (R2adj=77.5%, RMSE=0.10 g cm-3, MAE=0.08 g cm-3), showing that missing data on soil BD of Croatian Pseudogleys (or other similar soils, formed on similar parent materials) can be estimated reasonably well by using it. However, care should be taken when dealing with arable soils, which have different properties, compared with the natural soils considered herein.

Soil water regime, as one of the key components of soil fertility, refers to the quantity, retention, and movement of soil water. Rather than through expensive and/or time-consuming measurements, it can be assessed from the field-observable morphological properties in the soil profile. Excessively wetted soils have a specific morphology, and are therefore often referred to as hydromorphic. Their morphology is caused by various soil redoximorphic features (RMFs), resulting from the reduction, translocation, and oxidation of iron and manganese oxides. Hydromorphic soils largely comprise Gleysols and Stagnosols (along with Gleyic Fluvisols) that are excessively wetted by groundwater, precipitation and/or flooded water. Their morphology is often described/analyzed with different terms/criteria in line with their global distribution. This complicates the comparison and classification of such soils and thus their use or reclamation. This review paper describes and compares common RMFs and explains their formation. It then proposes the revised Croatian terms for these features, which are in line with the terms used in the international soil classification systems of WRB and/or Soil Taxonomy. Furthermore, the criteria/rules used for diagnosing RMFs when classifying hydromorphic soils are critically reviewed. Finally, it is shown that a methodologically sound RMFs description can provide a quick insight into the crucial soil water regime parameters, such as location and duration of soil saturation, the origin of the excess soil water, recentness of excessive soil wetting, etc. However, depending on the research objectives and/or actual soil conditions, field soil description cannot always fully replace continuous field monitoring of the soil water regime and/or laboratory and micromorphological soil analyses.