Literature about the occurrence of microplastic in biological tissues has increased over the last few years. This review aims to synthesis the evidence on the preparation of biological tissues, chemical identification of microplastic and accumulation in tissues. Several microplastic's extraction approaches from biological tissues emerged (i.e., alkaline, acids, oxidizing and enzymatic). However, criteria used for the selection of the extraction method have yet to be clarified. Similarly, analytical methodologies for chemical identification often does not align with the size of particles. Furthermore, sizes of microplastics found in biological tissues are likely to be biologically implausible, due to the size of the biological barriers. From this review, it emerged that further assessment are required to determine whether microplastic particles were truly internalized, were in the vasculature serving these organs, or were an artefact of the methodological process. The importance of a standardisation of quality control/quality assurance emerged. Findings arose from this review could have a broad implication, and could be used as a basis for further investigations, to reduce artifact results and clearly assess the fate of microplastics in biological tissues.

The honeybee (Apis mellifera L.) has been used in several studies for monitoring the environmental health status in terms of pollution, due to its wide-ranging foraging flights. Based on this consideration, this study aimed to analyze heavy metal pollution in Molise Region (Italy), by investigating five sites characterized by different levels of contamination. Furthermore, the authors carried out a sampling activity for a long period, in order to obtain a complete dataset. In this way, detailed information about the status of the environments was able to be obtained. The main purpose of this work was to assess the health status of Molise Region and to confirm the suitability of honeybees as environmental bioindicators of heavy metal pollution, by analyzing their variability over time and space. Furthermore, the study compared the health status associated with contamination in terms of heavy metals with that in two different areas of Italy, using hierarchical cluster analysis and principal component analysis, to evaluate the correlation existing among the three different areas of Italy. Following the findings, the authors suggest the use of honeybees as a bioindicator for heavy metal pollution in air quality studies.

Over the years, anthropogenic sources have increasingly affected food quality. One of the most sensitive and nutritional matrices affected by chemical contamination is honey, due to the use of acaricides. Recently, the attention has moved to the presence of phthalates (PAEs) and bisphenol A (BP-A), molecules present in plastic materials used both in the production phase and in the conservation of honey. In this study, an analytical method for the simultaneous determination of PAEs (dimethyl phthalate DMP, diethyl phthalate DEP, diisobutyl phthalate DiBP, dibutyl phthalate DBP, bis(2-ethylhexyl) phthalate DEHP, and di-n-octyl-phthalate DnOP) and BP-A was developed. The extraction technique is the ultrasound-vortex-assisted dispersive liquid–liquid microextraction (UVA-DLLME), using 150 µL of toluene as an extraction solvent, followed by the gas chromatography coupled with ion trap mass spectrometry analysis (GC–IT/MS). The developed method is sensitive, reliable, and reproducible: it shows high correlation coefficients (R > 0.999); limits of detection (LODs) less than 11 ng·g⁻¹; limits of quantification (LOQs) less than 16 ng·g⁻¹; repeatability below 3.6%, except BP-A (11.6%); and accuracy below 4.8%, except BP-A (17.6%). The method was applied to 47 nectar honey samples for evidencing similarities among them. The chemometric approach based on Hierarchical Cluster Analysis and Principal Component Analysis evidenced some similitudes about sample origin as well as marked differences between PAE and BP-A sources.

The "Trojan horse effect" of microplastics for organic and inorganic contaminants is an interesting topic. So far, the scientific community has focused on microplastics strictly as contaminants, but their role as vectors is still undefined. Adsorption of pollutants follows the Freundlich model by physisorption mechanism. Furthermore, ages and types of microplastics influence the adsorption of pollutants onto microplastics. Moreover, natural particles, like algae particles, present in the environment can interfere in the adsorption mechanisms. Due to their chemical composition of mainly O and N, it has been suggested that natural particles have a stronger adsorption affinity for some pollutants. Furthermore, microplastic's role as vector of pollutants into organisms is controversial. In fact, it has been suggested that the release is species-specific. In T. japonicus, accumulation of Hg loaded onto microplastics increased by 2.5 times, whereas a reduction of bioavailability of Hg was observed in R. lens.

In recent years, human populations’ exposure to microplastics via foods is becoming a topic of concern. Although microplastics have been defined as “emerging contaminants”, their occurrence in the environment and food is quite dated. This systematic review aims to investigate the discrepancies which are characterizing the research in the microplastics field in foods, with particular regard to sample preparations, microplastics’ concentrations and their effect on humans. For the selection of papers, the PRISMA methodology was followed. Discrepancies in the methodological approaches emerged and in the expression of the results as well, underlying the urgency in the harmonization of the methodological approaches. Uncertainties are still present regarding the adverse effects of microplastics on the human body. The scientific evidence obtained thus far is, in fact, not sufficient to demonstrate a concrete negative effect. This review has clearly underlined the need to standardise laboratory approaches to obtain useful results for better food safety management.

In this paper, an analytical protocol was developed for the simultaneous determination of phthalates (di-methyl phthalate DMP, di-ethyl phthalate DEP, di-isobutyl phthalate DiBP, di-n-butyl phthalate DBP, bis-(2-ethylhexyl) phthalate DEHP, di-n-octyl phthalate DNOP) and bisphenol A (BPA). The extraction technique used was the ultrasound vortex assisted dispersive liquid–liquid microextraction (UVA-DLLME). The method involves analyte extraction using 75 µL of benzene and subsequent analysis by gas chromatography combined with ion trap mass spectrometry (GC-IT/MS). The method is sensitive, reliable, and reproducible with a limit of detection (LOD) below 13 ng g⁻¹ and limit of quantification (LOQ) below 22 ng g⁻¹ and the intra- and inter-day errors below 7.2 and 9.3, respectively. The method developed and validated was applied to six honey samples (i.e., four single-use commercial ones and two home-made ones. Some phthalates were found in the samples at concentrations below the specific migration limits (SMLs). Furthermore, the commercial samples were subjected to two different thermal stresses (24 h and 48 h at 40 °C) for evidence of the release of plastic from the containers. An increase in the phthalate concentrations was observed, especially during the first phase of the shock, but the levels were still within the limits of the regulations.