Pine wilt disease (PWD), caused by Bursaphelenchus xylophilus, is one of the most severe forest diseases worldwide. PWD causes devastating disasters to Chinese pine trees, seriously threatening forestry production and the forest ecological environment, and causes economic losses of over ten billion yuan per year to China. Previous studies have shown that the spread of PWD is closely related to climate factors. Today, PWD is spreading rapidly owing to abnormal climate changes. In order to provide a reference for controlling the spread of PWD in China, in this study, we accurately assessed the risk of the continued spread of PWD in Northeast China; a correlative species distribution model (MaxEnt) (RM = 1, AUC = 0.9904) was used to evaluate China’s climate suitability for PWD. The effects of climate factors on the spread of PWD in Northeast China were studied using Liaoning Province as an example by analyzing the relationship between the changes in average precipitation, average temperature, average relative humidity, average vapor pressure deficit, average wind speed, average sunshine duration and the area of the PWD epidemic over the past five years. These results suggest that with the change in climate, the areas suitable for PWD have expanded, and certain previously unsuitable areas for its distribution have become suitable. Temperature and precipitation were found to play key roles in the occurrence and damage of PWD, and hot and arid conditions favored the spread of PWD. It is recommended that for areas within the suitable range of PWD but not yet epidemic areas, quarantine should be strengthened to prevent the further spread of PWD. In addition, special attention should be paid to epidemic areas with high temperatures and arid while the monitoring of PWD should be strengthened to achieve the early detection and timely treatment of infected epidemic trees. Our results indicate that PWD undoubtedly poses a major threat to Northeast Chinese pine species if climate change proceeds as projected. In the future, more attention should be paid to monitoring the northward spread of PWD, and further studies should consider meteorological data forecasts, which could facilitate timely control measures.

Spotted-wing drosophila, Drosophila suzukii (Matsumura), is a worldwide quarantine pest that is currently undergoing a rapid range expansion in the Americas, Europe, and parts of Africa. It feeds and breeds on soft-skinned fruits such as raspberries, blueberries, and cherries, and can cause significant economic losses to fruit production. This study investigated the occurrence of D. suzukii and its wild host fruits and parasitoids in Liaoning, Northeast China for the first time. Sentinel traps were used to monitor D. suzukii adults, and suspected fruits were collected weekly in four different locations (Wafangdian, Faku, Fengcheng, and Shenyang). The results showed that D. suzukii were distributed in the sweet soft-skinned fruit-production areas of Liaoning, and raspberry was the most infested fruit. During the field survey, four species of wild berries from non-crop habitats were found infested by D. suzukii, and two species of parasitoids (Leptopilina japonica and Asobara japonica) were collected. D. suzukii adult-population dynamics throughout the survey period (June to October) were similar in different survey locations; adult fly populations increased and peaked in August, and then declined until the fly was no longer detectable in October.

Monochamus saltuarius is a vector of Bursaphelenchus xylophilus in Japan, South Korea, and the middle temperate zone of China. However, there are only a few reports on this species in China, and its biological characteristics are still unclear. In this study, we aimed to elucidate the larval development and adult flight period of M. saltuarius to provide a theoretical basis for the effective control of pine wilt disease in the middle temperate zone of China. Seven morphological variables of larvae were measured to determine the number of larval instars, and the adult specimens of M. saltuarius were collected from traps in forests to study the flight period of adults in Fushun, Liaoning, the epidemic center of pine wilt disease in the middle temperate zone of China. The results revealed that the full larval period of M. saltuarius was 279.6 d, and the larvae had five instar stages, with an average duration of 7.4, 14.3, 49.8, 83.6, and 124.5 d, respectively. Additionally, 78.4% of the overwintering larvae were fourth instar, and 21.6% were fifth instar larvae. We also found that the adults began to emerge from early May to late June, and the period was from early May to mid-August in the forest. During the investigations period from 2018 to 2020, the total number of adults captured was minimal at 744, and precipitation was highest at 291.54 mm in 2019. We characterized the larval instars and adult flight period of M. saltuarius, which is a prerequisite for developing appropriate management strategies. The results of this study will provide an important reference for the formulation of strategies to control M. saltuarius and pine wilt disease.

Monochamus saltuarius is a vector of Bursaphelenchus xylophilus and has caused massive mortality in pine trees in Japan, South Korea, and the middle temperate zone of China. In this study, the feeding preferences of M. saltuarius to four common host pine species in the middle temperate zone of China were investigated in a laboratory. The volatiles of the four host pine species were sampled and analyzed by gas chromatography and mass spectrometry. The responses of M. saltuarius to host pine tree volatiles were measured using a four-arm olfactometer. The results showed that: (1) the preference of M. saltuarius for Pine tabulaeformis was significantly higher than the other ones. (2) The composition and content of the volatiles of the four tested host pine twigs were different, and the relative content of α-pinene was the highest. (3) M. saltuarius was highly sensitive to α-pinene, β-pinene, limonene, and 3-carene, and the attracting effect was enhanced by the mixture of volatile components. This study provides a reference for the development and application of attractants for M. saltuarius. Our results would be helpful in controlling M. saltuarius and pine wilt disease.

This research aimed to investigate the oviposition preference of Monochamus saltuarius for different host trees and to determine whether it is capable of completing its life cycle in Larix kaempferi and Larix gmelinii subsp. olgensis. Male and female adults were placed in insect cages with the logs of Pinus tabuliformis, P. koraiensis, L. kaempferi, and L. gmelinii subsp. olgensis positioned in the four corners to determine the female beetles’ oviposition preference and the development of their offspring on the different host tree logs. First-instar larvae were also collected and introduced into logs of L. kaempferi and L. gmelinii subsp. olgensis to observe and measure their development. In addition, the phloem thickness, sapwood thickness, heartwood thickness, xylem density, and moisture content of these four host tree species were measured. The results showed that M. saltuarius larvae can develop into adults on L. kaempferi and L. gmelinii subsp. olgensis, but the lifespan and fecundity of adult M. saltuarius were lower than those observed for P. tabuliformis. In the choice test, females preferred P. tabuliformis to oviposit (75.44%), followed by P. koraiensis (12.86%), which showed less preference for L. kaempferi (5.99%) or L. gmelinii subsp. olgensis (5.71%). Phloem thickness and xylem density were significantly greater in the logs of L. kaempferi and L. gmelinii subsp. olgensis than in those of P. tabuliformis and P. koraiensis. M. saltuarius may feed on Larix species when food sources are scarce in forests. However, the likelihood of the beetle feeding on Larix species may decrease in the presence of favorable host species.