To ensure food security on sustainable basis, reducing weeds interference and boosting land use efficiency are critical. A field study was conducted at research farm of University of Agriculture Faisalabad, Pakistan, to sort out the most productive maize-gram intercropping system under semi-arid environment. Treatments included sole maize in single row (60 cm apart) (T₁) and double rows (90 cm apart) (T₂) strips, sole black (T₃) and green gram (T₄) crops, six single rows (60 cm apart) of maize with twelve double rows (20 cm) of black (T₅) and green gram (T₆), three double rows (90 cm apart) of maize with three sets of quadratic rows (20 cm apart) of black (T₇) and green gram (T₈). The experiment was executed in regular arrangement of randomized complete block design with three replications. The results revealed that T₁ produced the highest grain yield (6.97 t ha⁻¹) of maize and significantly lower weeds infestation compared to wider row spacing (T₂). Among intercropping systems, T₈ significantly decreased weeds density (16.33 plants m⁻²) and their fresh (20.93 g m⁻²) and dry weights (5.63 g m⁻²), while the maximum land use efficiency as indicated by unmatched land equivalent ratio and intercropping advantage were recorded by T₇ and T₈. Interestingly, green gram in intercropping recorded over 58% higher productivity than black gram. We conclude that maize-green gram intercropping hold potential to impart sustainability to maize production by reducing weeds infestation (431% lower than sole maize) and could be a viable option for smallholder farmers in semi-arid environment.

Sugarcane crop constitutes one of the most vital sources of sugar and bioenergy globally; however, higher level of polyploidy makes its genome editing an intricate task. Recently, genome editing has become easier with CRISPR/Cas9 system that uses Cas9 to target sequence-specific regions and introduce double-strand breaks into the target region. This technique has been successfully employed to develop new varieties of sugarcane having desired phenotypic and physiological traits. Several genes can be fused with the CRISPR/Cas9 system leading to successful metabolic engineering and biological improvement for ensuring sustainable enhancement in sugarcane production. This simple RNA-guided genome editing technique has become a revolutionary tool and innovative application in biology that might be effectively employed for inducing specified genomic modifications in plant tissues. This review attempts to synthesize fundamentals of genome editing techniques with an ultimate aim to find out opportunities and challenges of sugarcane genome editing. It also tends to summarize the advances and achievements of gene editing via CRISPR-based genome editing technique for numerous field crops. Lastly, the enormous potential of CRISPR/Cas9 for gene editing in sugarcane, major challenges and future perspectives have been objectively elaborated.

Organic manures are more preferred and environmentally friendly than chemical fertilizers for minimally contaminating soil, water and environmental resources, but the determination of right source of organic manures continues to remain an unexplored aspect. Considering the important issue, a multi-year field trial was carried out to determine the response of forage soybean to four sources of nutrients such as chemical fertilizers (IF), poultry litter (PL), bovine’s farm yard slurry (BFYS) and sewage sludge (SS) and their seven binary combinations (PL+BFYS, PL+SS, PL+IF, BFYS+SS, BFYS+IF, SS+IF and PL+BFYS+SS). Supplementation of organic manures with mineral fertilizers remained superior to their sole application, particularly BFYS + IF was found significantly (p≤0.05) superior for yielding the highest fresh biomass (23.9, 26.4 and 25.7 t ha^–1) with improved nutritional quality. The same combination of integrated fertilizer management also recorded higher sustainability as per sustainable forage yield index along with the highest net income and the benefit-cost ratio. PL and SS applied in conjunction with IF performed better than sole or binary application of organic manures. Therefore, BFYS + IF may be recommended for adoption to produce comparable forage yield and nutritional quality of soybean along with reducing dependency on chemical fertilizers.

Greenhouse tomato production is a growing business worldwide, and it is strongly promoted by bumblebee pollination. Although there are over 250 bumblebee species worldwide, very few species have been reared successfully for greenhouse tomato pollination. Those successfully managed species, especially Bombus terrestris, are shipped around the world for commercial use. However, managed bumblebees are known to escape greenhouse facilities, have established local populations, spread disease to local bumblebees, and are blamed for the declines of some indigenous bee species. An alternative to shipping exotic bumblebees around the world is to develop local species for greenhouse pollination. Such an approach has the dual benefits of creating a new industry of insect rearing while reducing threats to local bee communities. In this study, we successfully reared Bombus haemorrhoidalis, which is the most common bumblebee species in Northern Pakistan, in a laboratory and compared its effectiveness as a tomato pollinator with that of commercial B. terrestris in a greenhouse. We found that the effectiveness of B. haemorrhoidalis in tomato pollination in a greenhouse is very similar to that of B. terrestris when it comes to the fruit size, number of seeds, and fruit weight. Our study provides an example of how to rear a native bumblebee species to pollinate local crops, which is a method that could potentially substitute the importation of non-ingenious bumblebees.

Brassica is a genus of plants in the mustard family that includes cauliflower, sprouts, broccoli and cabbage. Plants of the brassica family are rich sources of biologically active substance. The beneficial effects of brassica vegetables on human health have been somewhat linked to phytochemicals. They prevent oxidative stress, induce detoxification enzymes, stimulate immune system and decrease the risk of cancers. Crucifers are the important winter crops grown widely in tropical and temperate regions of the world, giving yield of 50.7 million tons. It is cultivated around the year over an area of 8263 hectares in Karnataka with production of 23.63 tons per hectare. Cauliflower and cabbage are the most common crops throughout the world. Diamondback moth (DBM) caused losses of about 16 million dollars by causing a 2.5% damage annually. There are many insect pests that attacked these crops and most common are diamondback moth, tobacco cutworm, aphid, jassid, cabbage worm and many others. The most important of these insect pests is the diamondback moth Plutella xylostella also called cabbage moth that belongs to Plutellidae. There are many controlled strategies including chemical control, biological control, physical control and many other methods. This study contributes to the literature offering understandings about the insect pests of brassica and their best management techniques.

Aquatic insect fauna remains an important tool for bio indication of environmental disturbance, while maintaining a healthy aquatic system. The purpose of the study was to document and to identify the diversity and distribution patterns of aquatic insect, a highly ignored aspect from the Qatar. Following the standard procedures, the samples were collected from aquatic habitats during the period October 2015 to May 2017 on monthly basis. A total of 11,287 individuals, belonging to 6 orders were captured. Dipterans were the abundant with the percentages of 71.01 (n=8,015), while the lowest percentage was observed for Coleoptera 0.04 (n=05). Twelve insects families were identified, among these five were reported under Diptera, followed by Hemiptera (03), while Coleoptera, Tricoptera, Odonata, and Ephemeroptera were represented by single families. Among the selected localities, Dipterans were collected from 10 stations, followed by Hemiptera (9), Coleoptera (4), Odonota (4), Ephemeroptera (3) and Trichoptera (1) respectively. Among the water bodies samples, streams were the most preferred habitats (n=2,767), while drinking water pools were the least (27). Moreover, the highest Simpson diversity index of 1.48 and lowest of 0.47 was recorded for flooded sewage pool and plastic containers respectively, while the low evenness values were observed for ponds, and less than 1 Margalef’s diversity values were seen for all habitats. This study documents the patterns of the diversity and distribution of aquatic insects, and provides a baseline for the future studies from Qatar.