Cobalt is a transition metal located in the fourth row of the periodic table and is a neighbor of iron and nickel. It has been considered an essential element for prokaryotes, human beings, and other mammals, but its essentiality for plants remains obscure. In this article, we proposed that cobalt (Co) is a potentially essential micronutrient of plants. Co is essential for the growth of many lower plants, such as marine algal species including diatoms, chrysophytes, and dinoflagellates, as well as for higher plants in the familyFabaceaeorLeguminosae. The essentiality to leguminous plants is attributed to its role in nitrogen (N) fixation by symbiotic microbes, primarily rhizobia. Co is an integral component of cobalamin or vitamin B₁₂, which is required by several enzymes involved in N₂fixation. In addition to symbiosis, a group of N₂fixing bacteria known as diazotrophs is able to situate in plant tissue as endophytes or closely associated with roots of plants including economically important crops, such as barley, corn, rice, sugarcane, and wheat. Their action in N₂fixation provides crops with the macronutrient of N. Co is a component of several enzymes and proteins, participating in plant metabolism. Plants may exhibit Co deficiency if there is a severe limitation in Co supply. Conversely, Co is toxic to plants at higher concentrations. High levels of Co result in pale-colored leaves, discolored veins, and the loss of leaves and can also cause iron deficiency in plants. It is anticipated that with the advance of omics, Co as a constitute of enzymes and proteins and its specific role in plant metabolism will be exclusively revealed. The confirmation of Co as an essential micronutrient will enrich our understanding of plant mineral nutrition and improve our practice in crop production.

Phytophthora species are notorious pathogens of several economically important crop plants. Several general elicitors, commonly referred to as Pathogen-Associated Molecular Patterns (PAMPs), from Phytophthora spp. have been identified that are recognized by the plant receptors to trigger induced defense responses in a process termed PAMP-triggered Immunity (PTI). Adapted Phytophthora pathogens have evolved multiple strategies to evade PTI. They can either modify or suppress their elicitors to avoid recognition by host and modulate host defense responses by deploying hundreds of effectors, which suppress host defense and physiological processes by modulating components involved in calcium and MAPK signaling, alternative splicing, RNA interference, vesicle trafficking, cell-to-cell trafficking, proteolysis and phytohormone signaling pathways. In incompatible interactions, resistant host plants perceive effector-induced modulations through resistance proteins and activate downstream components of defense responses in a quicker and more robust manner called effector-triggered-immunity (ETI). When pathogens overcome PTI—usually through effectors in the absence of R proteins—effectors-triggered susceptibility (ETS) ensues. Qualitatively, many of the downstream defense responses overlap between PTI and ETI. In general, these multiple phases of Phytophthora-plant interactions follow the PTI-ETS-ETI paradigm, initially proposed in the zigzag model of plant immunity. However, based on several examples, in Phytophthora-plant interactions, boundaries between these phases are not distinct but are rather blended pointing to a PTI-ETI continuum.

The present study aimed to witness the plant–microbe interaction associated with salt tolerance in crops. We isolated the endophytic microbe from the root zone of halophytic grass. Later, the salt tolerance of the endophyte was tested in the saline medium and was identified using nucleotide sequencing (GenBank under the accession numbers: SUB9030920 AH1_AHK_ITS1 MW570850: SUB9030920 AH1_AHK_ITS4 MW570851). Rice and maize seeds were coated with identified endophyte Aspergillus terreus and were sown in separate plastic pots. Later 21-day-old seedlings were subjected to three NaCl concentrations, including 50, 100, and 150 mM salt stress. Under saline conditions, A. terreus showed a substantial increase in growth, biomass, relative water content, oxidative balance, and photochemical efficiency of rice and maize plants. The data reflected that the stimulation of gibberellic acid (GA) in treated leaves may be the main reason for the upregulation of photosynthesis and the antioxidant defense cascade. The data also depict the downregulation of oxidative damage markers malondialdehyde, hydrogen peroxide in rice and maize plants. Conclusively, salt-tolerant endophytic fungus A. terreus explicitly displayed the positive plant–microbe interaction by developing salt tolerance in rice and maize plants. Salt tolerance by endophytic fungus coincides with the enhanced GA concentration, which illustrated the stimulated physiological mechanism and gene in response to the extreme environmental crisis, resulting in improved crop productivity.

Introduction: Endophytic microorganisms are bacteria or fungi that inhabit plant internal tissues contributing to various biological processes of plants. Some endophytic microbes can promote plant growth, which are known as plant growth-promoting endophytes (PGPEs). There has been an increasing interest in isolation and identification of PGPEs for sustainable production of crops. This study was undertaken to isolate PGPEs from roots of a halophytic species Sesuvium portulacastrum L. and elucidate potential mechanisms underlying the plant growth promoting effect.Methods: Surface-disinfected seeds of S. portulacastrum were germinated on an in vitro culture medium, and roots of some germinated seedlings were contaminated by bacteria and fungi. From the contamination, an endophytic fungus called BF-F (a fungal strain isolated from bacterial and fungal contamination) was isolated and identified. The genome of BF-F strain was sequenced, its genome structure and function were analyzed using various bioinformatics software. Additionally, the effect of BF-F on plant growth promotion were investigated by gene cluster analyses.Results: Based on the sequence homology (99%) and phylogenetic analysis, BF-F is likely a new Cladosporium angulosum strain or possibly a new Cladosporium species that is most homologous to C. angulosum. The BF-F significantly promoted the growth of dicot S. portulacastrum and Arabidopsis as well as monocot rice. Whole genome analysis revealed that the BF-F genome has 29,444,740 bp in size with 6,426 annotated genes, including gene clusters associated with the tryptophan synthesis and metabolism pathway, sterol synthesis pathway, and nitrogen metabolism pathway. BF-F produced indole-3-acetic acid (IAA) and also induced the expression of plant N uptake related genes.Discussion: Our results suggest that BF-F is a novel strain of Cladosporium and has potential to be a microbial fertilizer for sustainable production of crop plants. The resulting genomic information will facilitate further investigation of its genetic evolution and its function, particularly mechanisms underlying plant growth promotion.

Abiotic stress is one of the major constraints which restrain plant growth and productivity by disrupting physiological processes and stifling defense mechanisms. Hence, the present work aimed to evaluate the sustainability of bio-priming salt tolerant endophytes for improving plant salt tolerance. Paecilomyces lilacinus KUCC-244 and Trichoderma hamatum Th-16 were obtained and cultured on PDA medium containing different concentrations of NaCl. The highest salt (500 mM) tolerant fungal colonies were selected and purified. Paecilomyces at 61.3 × 10^-6 conidia/ml and Trichoderma at about 64.9 × 10^-3 conidia/ml of colony forming unit (CFU) were used for priming wheat and mung bean seeds. Twenty- days-old primed and unprimed seedlings of wheat and mung bean were subjected to NaCl treatments at 100 and 200 mM. Results indicate that both endophytes sustain salt resistance in crops, however T. hamatum significantly increased the growth (141 to 209%) and chlorophyll content (81 to 189%), over unprimed control under extreme salinity. Moreover, the reduced levels (22 to 58%) of oxidative stress markers (H₂O₂ and MDA) corresponded with the increased antioxidant enzymes like superoxide dismutase (SOD) and catalase (CAT) activities (141 and 110%). Photochemical attributes like quantum yield (F_V/F_M) (14 to 32%) and performance index (PI) (73 to 94%) were also enhanced in bio-primed plants in comparison to control under stress. In addition, the energy loss (DI_O/RC) was considerably less (31 to 46%), corresponding with lower damage at PS II level in primed plants. Also, the increase in I and P steps of OJIP curve in T. hamatum and P. lilacinus primed plants showed the availability of more active reaction centers (RC) at PS II under salt stress in comparison to unprimed control plants. Infrared thermographic images also showed that bio-primed plants were resistant to salt stress. Hence, it is concluded that the use of bio-priming with salt tolerant endophytes specifically T. hamatum can be an effective approach to mitigate the salt stress cosnequences and develop a potential salt resistance in crop plants.

Soil salinity is an important environmental problem that seriously affects plant growth and crop productivity. Phytoremediation is a cost-effective solution for reducing soil salinity and potentially converting the soils for crop production. Sesuvium portulacastrum is a typical halophyte which can grow at high salt concentrations. In order to explore the salt tolerance mechanism of S. portulacastrum, rooted cuttings were grown in a hydroponic culture containing ½ Hoagland solution with or without addition of 400 mM Na for 21 days. Root and leaf samples were taken 1 h and 21 days after Na treatment, and RNA-Seq was used to analyze transcript differences in roots and leaves of the Na-treated and control plants. A large number of differentially expressed genes (DEGs) were identified in the roots and leaves of plants grown under salt stress. Several key pathways related to salt tolerance were identified through KEGG analysis. Combined with physiological data and expression analysis, it appeared that cyclic nucleotide gated channels (CNGCs) were implicated in Na uptake and Na⁺/H⁺ exchangers (NHXs) were responsible for the extrusion and sequestration of Na, which facilitated a balance between Na⁺ and K⁺ in S. portulacastrum under salt stress. Soluble sugar and proline were identified as important osmoprotectant in salt-stressed S. portulacastrum plants. Glutathione metabolism played an important role in scavenging reactive oxygen species. Results from this study show that S. portulacastrum as a halophytic species possesses a suite of mechanisms for accumulating and tolerating a high level of Na; thus, it could be a valuable plant species used for phytoremediation of saline soils.