Microbial fuel cell (MFC) technology offers an alternative means for producing energy from waste products. In this review, several characteristics of MFC technology that make it revolutionary will be highlighted. First, a brief history presents how bioelectrochemical systems have advanced, ultimately describing the development of microbial fuel cells. Second, the focus is shifted to the attributes that enable MFCs to work efficiently. Next, follows the design of various MFC systems in use including their components and how they are assembled, along with an explanation of how they work. Finally, microbial fuel cell designs and types of main configurations used are presented along with the scalability of the technology for proper application. The present review shows importance of design and elements to reduce energy loss for scaling up the MFC system including the type of electrode, shape of the single reactor, electrical connection method, stack direction, and modulation. These aspects precede making economically applicable large-scale MFCs (over 1 m³ scale) a reality.

Oxygen intrusion into the anode chamber through proton exchange membrane can result in positive redox conditions in fed-batch, two chamber MFCs at the end of a cycle when the substrate is depleted. A slight increase in dissolved oxygen to 0.3 mg/L during MFC operation was not found to adversely affect power generation over subsequent cycles if sufficient substrate (acetate) was provided. Purging the anode chamber with air or pure oxygen for up to 10 days and 10 hrs also did not affect power generation, as power rapidly returned to previous levels when the chamber was sparged with nitrogen gas. When MFCs are connected in series, voltage reversal can occur resulting in a positive voltage applied to the anode biofilm. To investigate if this adversely affected the bacteria, voltages of 1, 2, 3, 4, and 9 V, were applied for 1 hr to the MFC before reconnecting it back to a fixed external load (1,000 Ω). A voltage of <2 V did not affect power generation. However, applying 3 V resulted in a 15 h lag phase before recovery, and 9 V produced a 60 h lag phase suggesting substantial damage to the bacteria that required re-growth of bacteria in the biofilm. These results indicate that charge reversal will be a more serious problem than oxygen intrusion into the anode chamber for sustained performance of MFCs.

Microbial fuel cells (MFCs) have been shown to be capable of clean energy production through the oxidation of biodegradable organic waste using various bacterial species as biocatalysts. In this study we found Saccharomyces cerevisiae, previously known electrochemcially inactive or less active species, can be acclimated with an electron mediator thionine for electrogenic biofilm formation in MFC, and electricity production is improved with facilitation of electron transfer. Power generation of MFC was also significantly increased by thionine with both aerated and non-aerated cathode. With electrochemically active biofilm enriched with swine wastewater, MFC power increased more significantly by addition of thionine. The optimum mediator concentration was 500 mM of thionine with S. cerevisae in MFC with the maximum voltage and current generation in the microbial fuel cell were 420 mV and 700 mA/m², respectively. Cyclic voltametry shows that thionine improves oxidizing and reducing capability in both pure culture and acclimated biofilm as compared to non-mediated cell. The results obtained indicated that thionine has great potential to enhance power generation from unmediated yeast or electrochemically active biofilm in MFC.

Biological treatment systems face many challenges in winter to reduce the level of nitrogen due to low temperatures. The present work aimed to study an electrochemical treatment to investigate the effect of applying an electric voltage to wastewater to reduce the ammonium nitrogen and COD (chemical oxygen demand) in domestic wastewater. This was done by using an electrochemical process in which a platinum-coated titanium material was used as an anode and stainless steel was used as a cathode (25 cm² electrode area/500 mL). Our results indicated that the removal of ammonium nitrogen (NH₄⁺–N) and the lowering of COD was directly proportional to the amount of electric voltage applied between the electrodes. Our seven hour experiment showed that 97.6% of NH₄⁺–N was removed at an electric voltage of 5 V, whereas only 68% was removed with 3 V, 20% with 1.2 V, and 10% with 0.6 V. Similarly, at 5 V, the removal of COD was around 97.5%. Over the seven hours of the experiment, the pH of wastewater increased from pH 7.12 to pH 8.15 when 5 V was applied to the wastewater. Therefore, electric voltage is effective in the oxidation of ammonium nitrogen and the reduction in COD in wastewater.