Quantifying the organic carbon content of soil over large areas is essential for characterising the soil and the effects of its management. However, analytical methods can be laborious and costly. Reflectance spectroscopy is a well-established and widespread method for estimating the chemical-element content of soils. The aim of this study was to estimate the soil organic carbon (SOC) content using hyperspectral remote sensing. The data were from soils from two localities in the semi-arid region of Brazil. The spectral reflectance factors of the collected soil samples were recorded at wavelengths ranging from 350–2500 nm. Pre-processing techniques were employed, including normalisation, Savitzky–Golay smoothing and first-order derivative analysis. The data (n = 65) were examined both jointly and by soil class, and subdivided into calibration and validation to independently assess the performance of the linear methods. Two multivariate models were calibrated using the SOC content estimated in the laboratory by principal component regression (PCR) and partial least squares regression (PLSR). The study showed significant success in predicting the SOC with transformed and untransformed data, yielding acceptable-to-excellent predictions (with the performance-to-deviation ratio ranging from 1.40–3.38). In general, the spectral reflectance factors of the soils decreased with the increasing levels of SOC. PLSR was considered more robust than PCR, whose wavelengths from 354 to 380 nm, 1685, 1718, 1757, 1840, 1876, 1880, 2018, 2037, 2042, and 2057 nm showed outstanding absorption characteristics between the predicted models. The results found here are of significant practical value for estimating SOC in Neosols and Cambisols in the semi-arid region of Brazil using VIS-NIR-SWIR spectroscopy.

Understanding organic carbon and predominant humic fractions in the soil allows contributes to soil quality management. Conventional fractionation techniques require time, excessive sampling, and high maintenance costs. In this study, predictive models for organic carbon in humic substances (HS) were evaluated using hyperspectral data as an alternative to chemical fractionation and quantification by wet digestion. Twenty-nine samples of Neossolos Flúvicos (Fluvents) - A1, and 36 samples of Cambissolos (Inceptisols) - A2 were used. The samples were also analyzed jointly, creating a third sample group - A1&A2. Untransformed spectral reflectance factors were obtained using the FieldSpec Pro FR 3 hyperspectral sensor (350–2500 nm). Pre-processing techniques were employed, including Savitzky–Golay smoothing and first- and second-order derivative analysis. After selecting variables using the Backward method, which removes spectral variables that are not statistically significant for the regression. Estimation models were built by Principal Components Regression (PCR) and Partial Least Squares Regression (PLSR). The spectral data were evaluated individually for soil classes A1 and A2, and jointly for A1&A2. The PLSR was more efficient than PCR, especially for the estimation models that used the first derivative of reflectance employing the three sample groups. For samples of A1, the best estimate was seen for humic acid (RPD = 6.09) and humin (RPD = 2.38); for A2, the best models estimated the OC in fulvic acid (RPD = 2.35) and humin (RPD = 2.51); and for the joint spectral data (A1&A2), the prediction was robust for humin only (RPD = 2.01). The most representative wavelengths were observed using the first derivative with PLSR and PCR, centred on the region between 1600 and 1800 nm. The first-derivative of reflectance calculated more-robust predictive models using PLSR than PCR. The best predictions occurred for organic carbon associated with humic acid in Neossolos Flúvicos, with fulvic acid in Cambissolos, and with humin in both soil classes.

This study presents the MSPAT (Multispectral Soil Plant Analysis Tool), a device designed for assessing leaf nitrogen concentrations in maize crops under field conditions. The MSPAT includes the AS7265x sensor, which has 18 bands and covers the spectrum from 410 to 940 nm. This device was designed to be portable, using the ESP32 microcontroller and incorporating such functionalities as data storage on a MicroSD card, communication with a smartphone via Wi-Fi, and geolocation of acquired data. The MSPAT was evaluated in an experiment conducted at the Federal University of Ceará (UFC), where maize was subjected to different doses of nitrogen fertiliser (0, 60, 90, 120, 150, and 180 kg·ha⁻¹ N). Spectral readings were taken at three phenological stages (V5, V10, and R2) using the MSPAT, an SPAD-502 chlorophyll meter, and a FieldSpec PRO FR3 spectroradiometer. After the optical measurements were taken, the nitrogen concentrations in the leaves were determined in a laboratory by using the Kjeldahl method. The data analysis included the calculation of normalised ratio indices (NRIs) using linear regression and the application of multivariate statistical methods (PLSR and PCR) for predicting leaf nitrogen concentrations (LNCs). The best performance for the MSPAT index (NRI) was obtained using the 900 nm and the 560 nm bands (R² = 0.64) at stage V10. In the validation analysis, the MSPAT presented an R² of 0.79, showing performance superior to that of SPAD-502, which achieved an R² of 0.70. This confirms the greater potential of the MSPAT compared to commercial equipment and makes it possible to obtain results similar to those obtained using the reference spectroradiometer. The PLSR model with data from the FieldSpec 3 provided important validation metrics when using reflectance data with first-derivative transformation (R² = 0.88, RMSE = 1.94 and MAE = 1.28). When using the MSPAT, PLSR (R² = 0.75, RMSE = 2.77 and MAE = 2.26) exhibited values of metrics similar to those for PCR (R² = 0.75, RMSE = 2.78 and MAE = 2.26). This study validates the use of MSPAT as an effective tool for monitoring the nutritional status of maize to optimize the use of nitrogen fertilisers.