The negative impacts on the ecosystem of antibiotic residues in the environment have become a global concern. However, little is known about the transformation mechanism of antibiotics by manganese peroxidase (MnP) from microorganisms. This work investigated the transformation characteristics, the antibacterial activity of byproducts, and the degradation mechanism of tetracycline (TC) by purified MnP from Phanerochaete chrysosporium. The results show that nitrogen-limited and high level of Mn²⁺ medium could obtain favorable MnP activity and inhibit the expression of lignin peroxidase by Phanerochaete chrysosporium. The purified MnP could transform 80% tetracycline in 3 h, and the threshold of reaction activator (H₂O₂) was about 0.045 mmol L⁻¹. After the 3rd cyclic run, the transformation rate was almost identical at the low initial concentration of TC (77.05–88.47%), while it decreased when the initial concentration was higher (49.36–60.00%). The antimicrobial potency of the TC transformation products by MnP decreased throughout reaction time. We identified seven possible degradation products and then proposed a potential TC transformation pathway, which included demethylation, oxidation of the dimethyl amino, decarbonylation, hydroxylation, and oxidative dehydrogenation. These findings provide a novel comprehension of the role of MnP on the fate of antibiotics in nature and may develop a potential technology for tetracycline removal.

Antibiotics are commonly used to prevent and control aquaculture diseases. However, long-term overuse of antibiotics not only leaves residues but also leads to changes in the nitrogen cycle in water, which threatens the survival of aquaculture organisms. The current results showed that sulfamethoxazole had no significant effect on the nitrogen cycle process in the actual aquaculture concentration. The inhibitory effect of 1.05 mg/L norfloxacin on ammonia-oxidizing bacteria was significantly greater than that on ammonia-oxidizing archaea, and the gene abundance of AOB amoA on the 14th day increased by 2.48 times compared with the 7th day. Under the influence of 3.9 mg/L oxytetracycline, the gene abundance of AOB amoA decreased significantly, while the number of AOA amoA genes increased, suggesting that there may be functional redundancy between AOA and AOB. At the genus level in the norfloxacin group, the relative abundance of Sva0485 increased by 14.0% on the 7th day compared with the control group but decreased 12.77% in the addition group. The relative abundance of Firmicutes, another dominant species in the oxytetracycline group, was 25.9%. This study shows that the addition of antibiotics may have a negative effect on the nitrogen-cycling microorganisms in aquaculture water.

Heavy metal pollution poses a significant threat to aquatic ecosystems and drinking water sources, necessitating comprehensive environmental assessments. This study investigated the spatial distribution, potential ecological risks, and sources of heavy metals in the surface sediments of Wanfeng Lake to inform effective pollution management strategies. Twelve sediment samples were collected and analyzed for eight heavy metals (Hg, As, Cu, Pb, Cd, Cr, Ni, Zn) using inductively coupled plasma mass spectrometry. The geo-accumulation index (I_geo) and potential ecological risk index (RI) were applied to assess contamination levels and ecological risks. Cluster analysis and Kriging interpolation were used to identify potential pollution sources and spatial patterns. Results revealed that heavy metal concentrations decreased in the order Zn > Pb > Cu > Cr > Ni > As > Cd > Hg, with Hg concentrations exceeding the national average for Chinese lake sediments. Ecological risk assessments identified Hg (0.06 μg/g) and Cd (0.10 μg/g) as the priority pollutants. The Hg posed a moderate ecological risk, particularly in upstream areas, while Cd pollution was concentrated downstream. Atmospheric deposition was identified as the primary source of Hg, whereas agricultural activities were determined to be the main driver of Cd contamination. These findings provide a scientific basis for developing targeted pollution control measures in Wanfeng Lake.